Bhi03G001224 (gene) Wax gourd (B227) v1

BLAST of Bhi03G001224 vs. TAIR 10
Match: AT3G53260.1 (phenylalanine ammonia-lyase 2 )

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 580/692 (83.82%), Postives = 640/692 (92.49%), Query Frame = 0

Query: 22  DPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVELSE 81
           DPLNWG+AAD +KGSHLDEVK M+EEYRRP+V +GGE+LTI QVAAI+T    V VEL+E
Sbjct: 26  DPLNWGLAADQMKGSHLDEVKKMVEEYRRPVVNLGGETLTIGQVAAISTVGGSVKVELAE 85

Query: 82  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 141
           ++RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK G ALQ ELIRFLNAGIFGN 
Sbjct: 86  TSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNT 145

Query: 142 AESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITASG 201
            E+ HTLP SATRAAMLVR+NTLLQGYSGIRFEILEAIT LLNHNI+P LPLRGTITASG
Sbjct: 146 KETCHTLPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLRGTITASG 205

Query: 202 DLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVNGTAV 261
           DLVPLSYIAGLLTGRPNSKA GP+GE + AKEAF++AGI +GFF+LQPKEGLALVNGTAV
Sbjct: 206 DLVPLSYIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPKEGLALVNGTAV 265

Query: 262 GSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI 321
           GSG+AS+VLFEAN+ AVL+E+LSAIFAEVM GKPEFTDHLTH+LKHHPGQIEAAAIMEHI
Sbjct: 266 GSGMASMVLFEANVQAVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHI 325

Query: 322 LDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDN 381
           LDGSSYMK A+K+HE+DPLQKPKQDRYALRTSPQWLGP IEVIR +TKSIEREINSVNDN
Sbjct: 326 LDGSSYMKLAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDN 385

Query: 382 PLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLS 441
           PLIDVSRNKA+HGGNFQGTPIGVSMDNTRLAIA+IGKLMFAQFSELVNDFYNNGLPSNL+
Sbjct: 386 PLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLT 445

Query: 442 ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEA 501
           AS NPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKT+EA
Sbjct: 446 ASSNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEA 505

Query: 502 IDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGALHPSRFCEK 561
           +DILKLMS+TFLV +CQA+DLRHLEENL+ TVK+TVSQVAKKVLTT  NG LHPSRFCEK
Sbjct: 506 VDILKLMSTTFLVGICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELHPSRFCEK 565

Query: 562 DLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIFQKIVLFEE 621
           DLLKVVDRE  F Y+DDPC+ATYPL Q+LRQV+V+HAL+NG+ EKNA TSIFQKI  FEE
Sbjct: 566 DLLKVVDREQVFTYVDDPCSATYPLMQRLRQVIVDHALSNGETEKNAVTSIFQKIGAFEE 625

Query: 622 ELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGEKVISPGEE 681
           ELKA+LPKEVE AR AY  G + I N+IK+CRSYP+YRFVREEL TK+LTGEKV+SPGEE
Sbjct: 626 ELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVSPGEE 685

Query: 682 CEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
            +KVF A+C+GK+ID +++CLKEWNGAPIPIC
Sbjct: 686 FDKVFTAMCEGKLIDPLMDCLKEWNGAPIPIC 717

BLAST of Bhi03G001224 vs. TAIR 10
Match: AT2G37040.1 (PHE ammonia lyase 1 )

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 580/700 (82.86%), Postives = 642/700 (91.71%), Query Frame = 0

Query: 14  ESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDS 73
           +++ I+  DPLNWG AA+ +KGSHLDEVK M+ E+R+P+V +GGE+LTI QVAAI+T  +
Sbjct: 26  KNMVINAEDPLNWGAAAEQMKGSHLDEVKRMVAEFRKPVVNLGGETLTIGQVAAISTIGN 85

Query: 74  DVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 133
            V VELSE+ARAGV ASSDWVMESMNKGTDSYGVTTGFGATSHRRTK G ALQKELIRFL
Sbjct: 86  SVKVELSETARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFL 145

Query: 134 NAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 193
           NAGIFG+  E+ HTLPHSATRAAMLVRINTLLQG+SGIRFEILEAIT  LN+NITP LPL
Sbjct: 146 NAGIFGSTKETSHTLPHSATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPL 205

Query: 194 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGL 253
           RGTITASGDLVPLSYIAGLLTGRPNSKA GPNGE + A+EAF+ AGI SGFF+LQPKEGL
Sbjct: 206 RGTITASGDLVPLSYIAGLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPKEGL 265

Query: 254 ALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 313
           ALVNGTAVGSG+AS+VLFE N+L+VL+EILSA+FAEVM GKPEFTDHLTH+LKHHPGQIE
Sbjct: 266 ALVNGTAVGSGMASMVLFETNVLSVLAEILSAVFAEVMSGKPEFTDHLTHRLKHHPGQIE 325

Query: 314 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 373
           AAAIMEHILDGSSYMK A+KLHE+DPLQKPKQDRYALRTSPQWLGP IEVIR++TKSIER
Sbjct: 326 AAAIMEHILDGSSYMKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRYATKSIER 385

Query: 374 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 433
           EINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLAIA+IGKLMFAQFSELVNDFYN
Sbjct: 386 EINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYN 445

Query: 434 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 493
           NGLPSNL+ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 446 NGLPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 505

Query: 494 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGAL 553
           SSRKT+EA+DILKLMS+TFLVA+CQA+DLRHLEENL+ TVK+TVSQVAKKVLTT  NG L
Sbjct: 506 SSRKTSEAVDILKLMSTTFLVAICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGEL 565

Query: 554 HPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIF 613
           HPSRFCEKDLLKVVDRE  + Y DDPC+ATYPL QKLRQV+V+HAL NG+ EKNA TSIF
Sbjct: 566 HPSRFCEKDLLKVVDREQVYTYADDPCSATYPLIQKLRQVIVDHALINGESEKNAVTSIF 625

Query: 614 QKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGE 673
            KI  FEEELKA+LPKEVE AR AY+ G S I N+IK+CRSYP+YRFVREEL T++LTGE
Sbjct: 626 HKIGAFEEELKAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELLTGE 685

Query: 674 KVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           KV SPGEE +KVF A+C+GK+ID ++ECL EWNGAPIPIC
Sbjct: 686 KVTSPGEEFDKVFTAICEGKIIDPMMECLNEWNGAPIPIC 725

BLAST of Bhi03G001224 vs. TAIR 10
Match: AT3G10340.1 (phenylalanine ammonia-lyase 4 )

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 559/693 (80.66%), Postives = 620/693 (89.47%), Query Frame = 0

Query: 22  DPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVELSE 81
           DPLNW   A++LKGSHLDEVK M++EYR+  VK+GGE+LTI QVAA+A       VEL+E
Sbjct: 16  DPLNWNATAEALKGSHLDEVKRMVKEYRKEAVKLGGETLTIGQVAAVARGGGGSTVELAE 75

Query: 82  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 141
            ARAGVKASS+WVMESMN+GTDSYGVTTGFGATSHRRTKQGGALQ ELIRFLNAGIFG G
Sbjct: 76  EARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQNELIRFLNAGIFGPG 135

Query: 142 A-ESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITAS 201
           A ++ HTLP   TRAAMLVR+NTLLQGYSGIRFEILEAITKLLNH ITPCLPLRGTITAS
Sbjct: 136 AGDTSHTLPKPTTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEITPCLPLRGTITAS 195

Query: 202 GDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVNGTA 261
           GDLVPLSYIAGLLTGRPNSKA+GP+GE + A EAF+ AG+ S FFELQPKEGLALVNGTA
Sbjct: 196 GDLVPLSYIAGLLTGRPNSKAVGPSGETLTASEAFKLAGV-SSFFELQPKEGLALVNGTA 255

Query: 262 VGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 321
           VGSGLAS VLF+ANILAVLSE++SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH
Sbjct: 256 VGSGLASTVLFDANILAVLSEVMSAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 315

Query: 322 ILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVND 381
           ILDGSSY+K A+ LHE+DPLQKPKQDRYALRTSPQWLGP IEVIR +TK IEREINSVND
Sbjct: 316 ILDGSSYVKEAQLLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVND 375

Query: 382 NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNL 441
           NPLIDVSRNKALHGGNFQGTPIGV+MDN+RLAIASIGKLMFAQFSELVNDFYNNGLPSNL
Sbjct: 376 NPLIDVSRNKALHGGNFQGTPIGVAMDNSRLAIASIGKLMFAQFSELVNDFYNNGLPSNL 435

Query: 442 SASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAE 501
           S  RNPSLDYGFKGAEIAMASYCSELQ+LANPVT+HVQSAEQHNQDVNSLGLISSRKTAE
Sbjct: 436 SGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAE 495

Query: 502 AIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGALHPSRFCE 561
           A+DILKLMS+T+LVALCQA+DLRHLEENLK  VKS VSQVAK+VLT  +NG LHPSRF E
Sbjct: 496 AVDILKLMSTTYLVALCQAVDLRHLEENLKKAVKSAVSQVAKRVLTVGANGELHPSRFTE 555

Query: 562 KDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIFQKIVLFE 621
           +D+L+VVDREY F+Y DDPC+ TYPL QKLR +LV+HALA+ + E N+ TS+F KI  FE
Sbjct: 556 RDVLQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHALADPEREANSATSVFHKIGAFE 615

Query: 622 EELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGEKVISPGE 681
            ELK LLPKEVE  R+ YE G S I N+IK+CRSYP+YRFVR+EL T++LTGE V SPGE
Sbjct: 616 AELKLLLPKEVERVRVEYEEGTSAIANRIKECRSYPLYRFVRDELNTELLTGENVRSPGE 675

Query: 682 ECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           E +KVF+A+  GK+ID +LECLKEWNGAP+ IC
Sbjct: 676 EFDKVFLAISDGKLIDPLLECLKEWNGAPVSIC 707

BLAST of Bhi03G001224 vs. TAIR 10
Match: AT5G04230.1 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 988.0 bits (2553), Expect = 3.9e-288
Identity = 510/696 (73.28%), Postives = 580/696 (83.33%), Query Frame = 0

Query: 21  NDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVELS 80
           +DPLNW VAA++LKGSHL+EVK M+++YR+  V++GGE+LTI QVAA+A+      VELS
Sbjct: 12  SDPLNWNVAAEALKGSHLEEVKKMVKDYRKGTVQLGGETLTIGQVAAVAS--GGPTVELS 71

Query: 81  ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGN 140
           E AR GVKASSDWVMESMN+ TD+YG+TTGFG++S RRT QG ALQKELIR+LNAGIF  
Sbjct: 72  EEARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFAT 131

Query: 141 GAESD---HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTI 200
           G E D   +TLP  ATRAAML+R+NTLLQGYSGIRFEILEAIT LLN  ITP LPLRGTI
Sbjct: 132 GNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTI 191

Query: 201 TASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVN 260
           TASGDLVPLSYIAG L GRPNS+++GP+GE + A EAF+ AG+ S FFEL+PKEGLALVN
Sbjct: 192 TASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGV-SSFFELRPKEGLALVN 251

Query: 261 GTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 320
           GTAVGS LAS VL++ANIL V SE+ SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI
Sbjct: 252 GTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 311

Query: 321 MEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINS 380
           MEHILDGSSY+K A  LH+IDPLQKPKQDRYALRTSPQWLGP IEVIR +TK IEREINS
Sbjct: 312 MEHILDGSSYVKEALHLHKIDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINS 371

Query: 381 VNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLP 440
           VNDNPLIDVSRNKA+HGGNFQGTPIGV+MDNTRLA+ASIGKLMFAQF+ELVNDFYNNGLP
Sbjct: 372 VNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYNNGLP 431

Query: 441 SNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRK 500
           SNLS  RNPSLDYG KGAE+AMASYCSELQ+LANPVT+HV+SA QHNQDVNSLGLISSR 
Sbjct: 432 SNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLISSRT 491

Query: 501 TAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGALHPSR 560
           TAEA+ ILKLMS+T+LVALCQA DLRHLEE LK  V   VS  AK VL      A+ P R
Sbjct: 492 TAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL------AIEPFR 551

Query: 561 FCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIFQKIV 620
               D+L VV+REY F+Y+DDP + T PL QKLR VL + ALA  + E +   ++F+KI 
Sbjct: 552 -KHDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKALAEPEGETD---TVFRKIG 611

Query: 621 LFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGEKVIS 680
            FE ELK LLPKEVE  R  YE G   + N+IK CRSYP+YRFVR ELET++LTGE V S
Sbjct: 612 AFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTGEDVRS 671

Query: 681 PGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           PGE+ +KVF A+ QGK+ID + ECLKEWNGAPI IC
Sbjct: 672 PGEDFDKVFRAISQGKLIDPLFECLKEWNGAPISIC 694

BLAST of Bhi03G001224 vs. TAIR 10
Match: AT5G04230.2 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 982.2 bits (2538), Expect = 2.1e-286
Identity = 510/700 (72.86%), Postives = 580/700 (82.86%), Query Frame = 0

Query: 21  NDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVELS 80
           +DPLNW VAA++LKGSHL+EVK M+++YR+  V++GGE+LTI QVAA+A+      VELS
Sbjct: 12  SDPLNWNVAAEALKGSHLEEVKKMVKDYRKGTVQLGGETLTIGQVAAVAS--GGPTVELS 71

Query: 81  ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGN 140
           E AR GVKASSDWVMESMN+ TD+YG+TTGFG++S RRT QG ALQKELIR+LNAGIF  
Sbjct: 72  EEARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFAT 131

Query: 141 GAESD---HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTI 200
           G E D   +TLP  ATRAAML+R+NTLLQGYSGIRFEILEAIT LLN  ITP LPLRGTI
Sbjct: 132 GNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTI 191

Query: 201 TASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVN 260
           TASGDLVPLSYIAG L GRPNS+++GP+GE + A EAF+ AG+ S FFEL+PKEGLALVN
Sbjct: 192 TASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGV-SSFFELRPKEGLALVN 251

Query: 261 GTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 320
           GTAVGS LAS VL++ANIL V SE+ SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI
Sbjct: 252 GTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 311

Query: 321 MEHILDGSSYMKTAKKLHEIDPLQKPKQDR----YALRTSPQWLGPLIEVIRFSTKSIER 380
           MEHILDGSSY+K A  LH+IDPLQKPKQDR    YALRTSPQWLGP IEVIR +TK IER
Sbjct: 312 MEHILDGSSYVKEALHLHKIDPLQKPKQDRYVLGYALRTSPQWLGPQIEVIRAATKMIER 371

Query: 381 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 440
           EINSVNDNPLIDVSRNKA+HGGNFQGTPIGV+MDNTRLA+ASIGKLMFAQF+ELVNDFYN
Sbjct: 372 EINSVNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYN 431

Query: 441 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 500
           NGLPSNLS  RNPSLDYG KGAE+AMASYCSELQ+LANPVT+HV+SA QHNQDVNSLGLI
Sbjct: 432 NGLPSNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLI 491

Query: 501 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGAL 560
           SSR TAEA+ ILKLMS+T+LVALCQA DLRHLEE LK  V   VS  AK VL      A+
Sbjct: 492 SSRTTAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL------AI 551

Query: 561 HPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIF 620
            P R    D+L VV+REY F+Y+DDP + T PL QKLR VL + ALA  + E +   ++F
Sbjct: 552 EPFR-KHDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKALAEPEGETD---TVF 611

Query: 621 QKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGE 680
           +KI  FE ELK LLPKEVE  R  YE G   + N+IK CRSYP+YRFVR ELET++LTGE
Sbjct: 612 RKIGAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTGE 671

Query: 681 KVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
            V SPGE+ +KVF A+ QGK+ID + ECLKEWNGAPI IC
Sbjct: 672 DVRSPGEDFDKVFRAISQGKLIDPLFECLKEWNGAPISIC 698

BLAST of Bhi03G001224 vs. ExPASy Swiss-Prot
Match: P45730 (Phenylalanine ammonia-lyase OS=Populus trichocarpa OX=3694 GN=PAL PE=2 SV=1)

HSP 1 Score: 1217.6 bits (3149), Expect = 0.0e+00
Identity = 605/706 (85.69%), Postives = 664/706 (94.05%), Query Frame = 0

Query: 9   QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAI 68
           QNGS+ESLC++Q DPL+WGVAA+++KGSHLDEVK M+ +YR+P+VK+GGE+LTI+QVA+I
Sbjct: 10  QNGSLESLCVNQRDPLSWGVAAEAMKGSHLDEVKRMVADYRKPVVKLGGETLTIAQVASI 69

Query: 69  ATRDS-DVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQK 128
           A  D+ DV VELSESAR GVKASSDWVM+SM+KGTDSYGVTTGFGATSHRRTKQGGALQK
Sbjct: 70  AGHDTGDVKVELSESARPGVKASSDWVMDSMDKGTDSYGVTTGFGATSHRRTKQGGALQK 129

Query: 129 ELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNI 188
           ELIRFLNAGIFGNG E+ HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT+LLN+NI
Sbjct: 130 ELIRFLNAGIFGNGTETCHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITRLLNNNI 189

Query: 189 TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFEL 248
           TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA GP GE +DA EAF+ AGI SGFFEL
Sbjct: 190 TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKATGPTGEVLDAAEAFKAAGIESGFFEL 249

Query: 249 QPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKH 308
           QPKEGLALVNGTAVGSGLAS+VLFE N+LAVLSE+LSAIFAEVM GKPEFTDHLTHKLKH
Sbjct: 250 QPKEGLALVNGTAVGSGLASMVLFETNVLAVLSELLSAIFAEVMNGKPEFTDHLTHKLKH 309

Query: 309 HPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFS 368
           HPGQIEAAAIMEHILDGS+YMK AKKLHE DPLQKPKQDRYALRTSPQWLGP IEVIRFS
Sbjct: 310 HPGQIEAAAIMEHILDGSAYMKAAKKLHETDPLQKPKQDRYALRTSPQWLGPQIEVIRFS 369

Query: 369 TKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSEL 428
           TKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDN RLAIASIGKL+FAQFSEL
Sbjct: 370 TKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNVRLAIASIGKLLFAQFSEL 429

Query: 429 VNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDV 488
           VNDFYNNGLPSNL+ASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDV
Sbjct: 430 VNDFYNNGLPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTTHVQSAEQHNQDV 489

Query: 489 NSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTT 548
           NSLGLISSRKTAEA+DILKLMS+TFLVALCQAIDLRHLEENLKS VK+TVSQV+K+VLTT
Sbjct: 490 NSLGLISSRKTAEAVDILKLMSTTFLVALCQAIDLRHLEENLKSAVKNTVSQVSKRVLTT 549

Query: 549 CSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKN 608
            +NG LHPSRFCEK+LLKVVDREY FAY+DDPC+ATYPL QKLRQV V+HAL NG++EKN
Sbjct: 550 GANGELHPSRFCEKELLKVVDREYVFAYVDDPCSATYPLMQKLRQVFVDHALENGENEKN 609

Query: 609 ADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELET 668
             TS+FQKI  FEEELKALLPKEVE+AR AY++GNS I+N+IK+CRSYP+Y+FVREEL T
Sbjct: 610 FSTSVFQKIEAFEEELKALLPKEVESARAAYDSGNSAIDNKIKECRSYPLYKFVREELGT 669

Query: 669 KMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
            +LTGEKV SPGEE +KVF A+CQGK+ID +LECL EWNG+P+PIC
Sbjct: 670 VLLTGEKVQSPGEEFDKVFTAMCQGKIIDPMLECLGEWNGSPLPIC 715

BLAST of Bhi03G001224 vs. ExPASy Swiss-Prot
Match: P45732 (Phenylalanine ammonia-lyase OS=Stylosanthes humilis OX=35628 GN=PAL17.1 PE=2 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 598/694 (86.17%), Postives = 651/694 (93.80%), Query Frame = 0

Query: 20  QNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVEL 79
           ++DPLNW  AA++LKGSHLDEVK M+ EYR+P+V +GG++LTISQVAAIA  D  V V+L
Sbjct: 22  RHDPLNWAAAAEALKGSHLDEVKRMVSEYRKPLVNLGGQTLTISQVAAIAANDQGVSVQL 81

Query: 80  SESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG 139
           SE++RAGVKASSDWVM+SMN GTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG
Sbjct: 82  SEASRAGVKASSDWVMDSMNNGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG 141

Query: 140 NGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITA 199
           NG E++ TLPH+ATRAAMLVRINTLLQGYSGIRFEILEAITKLLN+NITPCLPLRGTITA
Sbjct: 142 NGTETNCTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITPCLPLRGTITA 201

Query: 200 SGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVNGT 259
           SGDLVPLSYIAGLLTGRPNSKA+GPNGE ++AKEAFQ AGI S FFELQPKEGLALVNGT
Sbjct: 202 SGDLVPLSYIAGLLTGRPNSKAVGPNGETLNAKEAFQAAGIGSDFFELQPKEGLALVNGT 261

Query: 260 AVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIME 319
            VGSGLAS+VLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIME
Sbjct: 262 PVGSGLASVVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIME 321

Query: 320 HILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVN 379
           HILDGSSY+K AKKLHEIDPLQKPKQDRYALRTSPQWLGPL+EVIRFSTKSIEREINSVN
Sbjct: 322 HILDGSSYVKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLVEVIRFSTKSIEREINSVN 381

Query: 380 DNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSN 439
           DNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKLMFAQFSELVNDFYNNGLPSN
Sbjct: 382 DNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSELVNDFYNNGLPSN 441

Query: 440 LSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTA 499
           LSASRNPSLDYGFKG EIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLIS+RKT 
Sbjct: 442 LSASRNPSLDYGFKGTEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISARKTN 501

Query: 500 EAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGALHPSRFC 559
           EA++ILKLMS T+L+ALCQAIDLRHLEENLK+TVK+TVSQVAK+ LTT  NG LHPSRFC
Sbjct: 502 EAVEILKLMSPTYLIALCQAIDLRHLEENLKNTVKNTVSQVAKRTLTTGVNGELHPSRFC 561

Query: 560 EKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIFQKIVLF 619
           EKDLLK+VDREY FAYIDDPC+ATYPL QKLRQVLVEHALAN ++EKN +TSIFQKI  F
Sbjct: 562 EKDLLKIVDREYCFAYIDDPCSATYPLMQKLRQVLVEHALANAENEKNVNTSIFQKITTF 621

Query: 620 EEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGEKVISPG 679
           EEELK LLPKEVE AR+AYE G S I N+IK+CRSYP+Y+FVREEL T+MLTGEKV SPG
Sbjct: 622 EEELKTLLPKEVEGARIAYENGQSAIPNKIKECRSYPLYKFVREELGTEMLTGEKVRSPG 681

Query: 680 EECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           EEC+K+F A+CQGK+ID +LEC+ EWNGAP+P+C
Sbjct: 682 EECDKLFTAMCQGKIIDPLLECIGEWNGAPLPLC 715

BLAST of Bhi03G001224 vs. ExPASy Swiss-Prot
Match: P45734 (Phenylalanine ammonia-lyase OS=Trifolium subterraneum OX=3900 GN=PAL1 PE=3 SV=1)

HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 601/704 (85.37%), Postives = 659/704 (93.61%), Query Frame = 0

Query: 10  NGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIA 69
           + +  ++ ++  DPLNWGVAA+++KGSHLDEVK M+EEYR+P+V++GGE+LTISQVAAIA
Sbjct: 23  HANANNMKVNAADPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIA 82

Query: 70  TRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKEL 129
             D    VELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKEL
Sbjct: 83  AHDG-ATVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKEL 142

Query: 130 IRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITP 189
           IRFLNAGIFGNG ES+HTLPH+ATRAAMLVRINTLLQGYSGIRFEILEAITKLLN+NITP
Sbjct: 143 IRFLNAGIFGNGTESNHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITP 202

Query: 190 CLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQP 249
           CLPLRGTITASGDLVPLSYIAGLLTGR NSKA GP+GE ++AKEAFQ AGI + FFELQP
Sbjct: 203 CLPLRGTITASGDLVPLSYIAGLLTGRSNSKAHGPSGEMLNAKEAFQLAGINAEFFELQP 262

Query: 250 KEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHP 309
           KEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHP
Sbjct: 263 KEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHP 322

Query: 310 GQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK 369
           GQIEAAAIMEHIL GS+Y+K AKKLHE+DPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK
Sbjct: 323 GQIEAAAIMEHILHGSAYVKDAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK 382

Query: 370 SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVN 429
           SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKL+FAQFSELVN
Sbjct: 383 SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVN 442

Query: 430 DFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNS 489
           DFYNNGLPSNLSASRNPSLDYGFKG+EIAMASYCSELQYLANPVT+HVQSAEQHNQDVNS
Sbjct: 443 DFYNNGLPSNLSASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNS 502

Query: 490 LGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCS 549
           LGLISSRKT EAI+IL+LMSSTFL+ALCQAIDLRHLEENLK++VK+TVSQVAKK LT   
Sbjct: 503 LGLISSRKTKEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNTVSQVAKKTLTIGV 562

Query: 550 NGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNAD 609
           +G LHPSRFCEKDLLKVVDRE+ F+YIDDPC+ATYPL+QKLRQVLV+HAL NG+ EKN++
Sbjct: 563 SGELHPSRFCEKDLLKVVDREHVFSYIDDPCSATYPLAQKLRQVLVDHALVNGESEKNSN 622

Query: 610 TSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKM 669
           TSIFQKI  FEEELK LLPKEVE+AR AYE GNS I N+I  CRSYP+Y+FVREEL T +
Sbjct: 623 TSIFQKIATFEEELKTLLPKEVESARTAYENGNSTIANKINGCRSYPLYKFVREELGTSL 682

Query: 670 LTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           LTGE+VISPGEEC+K+F A+CQGK+ID +L+CL EWNGAP+PIC
Sbjct: 683 LTGERVISPGEECDKLFTAMCQGKIIDPLLKCLGEWNGAPLPIC 725

BLAST of Bhi03G001224 vs. ExPASy Swiss-Prot
Match: Q04593 (Phenylalanine ammonia-lyase 2 OS=Pisum sativum OX=3888 GN=PAL2 PE=2 SV=1)

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 597/703 (84.92%), Postives = 657/703 (93.46%), Query Frame = 0

Query: 10  NGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIA 69
           N    ++ +S +DPLNWGVAA+++KGSHLDEVK M++EYR+P+V++GGE+LTISQVAAIA
Sbjct: 22  NAKNNNIKVSDSDPLNWGVAAEAMKGSHLDEVKRMVDEYRKPVVRLGGETLTISQVAAIA 81

Query: 70  TRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKEL 129
             D  V VELSESARAGVKASSDWVMESMNKGTDSYGVTT  GATSHRRTKQGGALQKEL
Sbjct: 82  AHDHGVKVELSESARAGVKASSDWVMESMNKGTDSYGVTTVHGATSHRRTKQGGALQKEL 141

Query: 130 IRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITP 189
           IRFLNAGIFGNG+ES HTLPH+ATRAAMLVRINTLLQGYSGIRFEILEAITKL+N+N+TP
Sbjct: 142 IRFLNAGIFGNGSESTHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLINNNVTP 201

Query: 190 CLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQP 249
           CL LRGTITASGDLVPLSYIAGLLTGRPNSKA GP+GE ++A+EAFQ AGI  GFFELQP
Sbjct: 202 CL-LRGTITASGDLVPLSYIAGLLTGRPNSKAHGPSGEILNAREAFQSAGINDGFFELQP 261

Query: 250 KEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHP 309
           KEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHP
Sbjct: 262 KEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHP 321

Query: 310 GQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK 369
           GQIEAAAIMEHILDGS+Y+K AKKLHE+DPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK
Sbjct: 322 GQIEAAAIMEHILDGSAYVKAAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK 381

Query: 370 SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVN 429
           SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKL+FAQFSELVN
Sbjct: 382 SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVN 441

Query: 430 DFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNS 489
           DFYNNGLPSNLSASRNPSLDYGFKG+EIAMASYCSELQYLANPVT+HVQSAEQHNQDVNS
Sbjct: 442 DFYNNGLPSNLSASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNS 501

Query: 490 LGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCS 549
           LGLISSRKT EAI+IL+LMSSTFL+ALCQAIDLRHLEENLK++VK+ VS VAK+ LTT  
Sbjct: 502 LGLISSRKTYEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNMVSHVAKRTLTTGI 561

Query: 550 NGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNAD 609
           NG LHPSRFCEKDLL+VVDRE+ F+YIDDPC+ATYPL QKLRQVLV+HAL NG+ EKN +
Sbjct: 562 NGELHPSRFCEKDLLRVVDREHVFSYIDDPCSATYPLMQKLRQVLVDHALVNGESEKNLN 621

Query: 610 TSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKM 669
           TSIFQKI  FE+ELK LLPKEVE+AR AYE GN+ I N+IK+CRSYP+Y+FVREEL T +
Sbjct: 622 TSIFQKIATFEDELKTLLPKEVESARGAYENGNTTISNKIKECRSYPLYKFVREELGTSL 681

Query: 670 LTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPI 713
           LTGEKVISPGEEC+K+F A+CQGK+ID +LECL +WNGAP+PI
Sbjct: 682 LTGEKVISPGEECDKLFTAICQGKIIDPLLECLGDWNGAPLPI 723

BLAST of Bhi03G001224 vs. ExPASy Swiss-Prot
Match: O64963 (Phenylalanine ammonia-lyase 1 OS=Prunus avium OX=42229 GN=PAL1 PE=2 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 600/707 (84.87%), Postives = 655/707 (92.64%), Query Frame = 0

Query: 9   QNGSME--SLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVA 68
           +NGS+E   LCI + DPLNWGVAA++LKGSHLDEVK M+ EYR+P+VK+GGESLTISQVA
Sbjct: 12  KNGSVELPELCI-KKDPLNWGVAAETLKGSHLDEVKRMVAEYRKPVVKLGGESLTISQVA 71

Query: 69  AIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQ 128
           AIAT DS V VELSESARAGVKASSDWVM+SM+KGTDSYGVTTGFGATSHRRTKQG ALQ
Sbjct: 72  AIATHDSGVKVELSESARAGVKASSDWVMDSMSKGTDSYGVTTGFGATSHRRTKQGAALQ 131

Query: 129 KELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHN 188
           KELIRFLNAG+FG+  ES HTLPH ATRAAMLVRINTLLQGYSGIRFEILE ITK LN+N
Sbjct: 132 KELIRFLNAGVFGSTKESGHTLPHQATRAAMLVRINTLLQGYSGIRFEILEVITKFLNNN 191

Query: 189 ITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFE 248
           +TPCLPLRGTITASGDLVPLSYIAG+LTGRPNSKA+GP+G+ + A EAF+  GI SGFFE
Sbjct: 192 VTPCLPLRGTITASGDLVPLSYIAGMLTGRPNSKAVGPDGQTLSAAEAFEFVGINSGFFE 251

Query: 249 LQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLK 308
           LQPKEGLALVNGTAVGSGLAS VLF+ NILA+LSEILSAIFAEVMQGKPEFTDHLTHKLK
Sbjct: 252 LQPKEGLALVNGTAVGSGLASTVLFDTNILALLSEILSAIFAEVMQGKPEFTDHLTHKLK 311

Query: 309 HHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRF 368
           HHPGQIEAAAIMEHILDGSSY+K AKKLHE DPLQKPKQDRYALRTSPQWLGP IEVIR+
Sbjct: 312 HHPGQIEAAAIMEHILDGSSYVKAAKKLHEQDPLQKPKQDRYALRTSPQWLGPQIEVIRY 371

Query: 369 STKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSE 428
           STKSIEREI+SVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSE
Sbjct: 372 STKSIEREIDSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSE 431

Query: 429 LVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQD 488
           LVNDFYNNGLPSNLS  RNPSLDYGFKGAEIAMASYCSELQ+LANPVT+HVQSAEQHNQD
Sbjct: 432 LVNDFYNNGLPSNLSGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQD 491

Query: 489 VNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLT 548
           VNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENL++TVK+TVSQVAK+ LT
Sbjct: 492 VNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLRNTVKNTVSQVAKRTLT 551

Query: 549 TCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEK 608
           T  NG LHPSRFCEKDLLKVVDREY FAYIDDPC+ATYPL QKLRQVLVEHAL NG++EK
Sbjct: 552 TGVNGELHPSRFCEKDLLKVVDREYVFAYIDDPCSATYPLMQKLRQVLVEHALTNGENEK 611

Query: 609 NADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELE 668
           NA TSIFQKIV FEEELK LLPKEV++AR A ++G++ + N+I +CRSYP+Y+FVREEL 
Sbjct: 612 NASTSIFQKIVAFEEELKVLLPKEVDSARAALDSGSAGVPNRITECRSYPLYKFVREELG 671

Query: 669 TKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
            + LTGEKV SPGEEC+KVF A+C+GK+ID IL+CL+ WNGAP+PIC
Sbjct: 672 AEYLTGEKVRSPGEECDKVFTAICEGKIIDPILDCLEGWNGAPLPIC 717

BLAST of Bhi03G001224 vs. NCBI nr
Match: XP_038881628.1 (phenylalanine ammonia-lyase-like [Benincasa hispida])

HSP 1 Score: 1404.8 bits (3635), Expect = 0.0e+00
Identity = 713/713 (100.00%), Postives = 713/713 (100.00%), Query Frame = 0

Query: 1   MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESL 60
           MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESL
Sbjct: 1   MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESL 60

Query: 61  TISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120
           TISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK
Sbjct: 61  TISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120

Query: 121 QGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180
           QGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT
Sbjct: 121 QGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180

Query: 181 KLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGI 240
           KLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGI
Sbjct: 181 KLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGI 240

Query: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300
           PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH
Sbjct: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300

Query: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360
           LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL
Sbjct: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360

Query: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420
           IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM
Sbjct: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420

Query: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480
           FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA
Sbjct: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480

Query: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQV 540
           EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQV
Sbjct: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQV 540

Query: 541 AKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALA 600
           AKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALA
Sbjct: 541 AKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALA 600

Query: 601 NGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRF 660
           NGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRF
Sbjct: 601 NGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRF 660

Query: 661 VREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           VREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 661 VREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 713

BLAST of Bhi03G001224 vs. NCBI nr
Match: XP_004143258.1 (phenylalanine ammonia-lyase [Cucumis sativus] >KGN48163.1 hypothetical protein Csa_004044 [Cucumis sativus])

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 676/713 (94.81%), Postives = 699/713 (98.04%), Query Frame = 0

Query: 1   MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESL 60
           MA  HFQQ+NGS+ESLCI  NDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GGE+L
Sbjct: 1   MAFAHFQQKNGSVESLCIGSNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETL 60

Query: 61  TISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120
           TISQVAAIATRD+DVIVELSES+RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK
Sbjct: 61  TISQVAAIATRDTDVIVELSESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120

Query: 121 QGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180
           QGGALQKELIRFLNAGIFG+GAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT
Sbjct: 121 QGGALQKELIRFLNAGIFGSGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180

Query: 181 KLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGI 240
           KLLN+NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGER+ AKEAFQQA I
Sbjct: 181 KLLNNNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERITAKEAFQQADI 240

Query: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300
           PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH
Sbjct: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300

Query: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360
           LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL
Sbjct: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360

Query: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420
           IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM
Sbjct: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420

Query: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480
           FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA
Sbjct: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480

Query: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQV 540
           EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVK+TVSQ+
Sbjct: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKTTVSQI 540

Query: 541 AKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALA 600
           AKKVLTT SNG LHPSRFCEKDLLKVVDREYTF+YIDDPC+ATYPLSQKLRQVLVEHALA
Sbjct: 541 AKKVLTTSSNGTLHPSRFCEKDLLKVVDREYTFSYIDDPCSATYPLSQKLRQVLVEHALA 600

Query: 601 NGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRF 660
           NGD+EK  DTSIFQKI  FEEELKA+LPKEVEN+RLAYE+G+SKIENQIK+CRSYP+YRF
Sbjct: 601 NGDNEKTVDTSIFQKIASFEEELKAVLPKEVENSRLAYESGSSKIENQIKNCRSYPLYRF 660

Query: 661 VREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           VREELETKMLTGEKVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 661 VREELETKMLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 713

BLAST of Bhi03G001224 vs. NCBI nr
Match: XP_038881051.1 (phenylalanine ammonia-lyase-like [Benincasa hispida])

HSP 1 Score: 1339.7 bits (3466), Expect = 0.0e+00
Identity = 678/702 (96.58%), Postives = 692/702 (98.58%), Query Frame = 0

Query: 13  MESLCISQ-NDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATR 72
           +E++C S  +DPLNWG+AA+SLKGSHLDEVK M+EEYRRP++K+GGE+LTISQVAAIATR
Sbjct: 4   IETICKSVIHDPLNWGIAAESLKGSHLDEVKRMVEEYRRPLIKLGGETLTISQVAAIATR 63

Query: 73  DSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR 132
           +SDV VELSESARAGV ASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR
Sbjct: 64  ESDVTVELSESARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIR 123

Query: 133 FLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCL 192
           FLNAGIFGNG ES HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCL
Sbjct: 124 FLNAGIFGNGTESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCL 183

Query: 193 PLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKE 252
           PLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGE +DAKEAFQQA IPSGFFELQPKE
Sbjct: 184 PLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAKEAFQQADIPSGFFELQPKE 243

Query: 253 GLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQ 312
           GLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQ
Sbjct: 244 GLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQ 303

Query: 313 IEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSI 372
           IEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSI
Sbjct: 304 IEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSI 363

Query: 373 EREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDF 432
           EREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDF
Sbjct: 364 EREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDF 423

Query: 433 YNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLG 492
           YNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLG
Sbjct: 424 YNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLG 483

Query: 493 LISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNG 552
           LISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNG
Sbjct: 484 LISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNG 543

Query: 553 ALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTS 612
           ALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTS
Sbjct: 544 ALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTS 603

Query: 613 IFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLT 672
           IFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLT
Sbjct: 604 IFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLT 663

Query: 673 GEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           GEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 664 GEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 705

BLAST of Bhi03G001224 vs. NCBI nr
Match: APO14269.1 (phenylalanine ammonia-lyase 3 [Luffa aegyptiaca])

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 669/700 (95.57%), Postives = 690/700 (98.57%), Query Frame = 0

Query: 14  ESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDS 73
           +SLC S+NDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GGE+LTISQVAAIATRDS
Sbjct: 9   DSLCHSRNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 68

Query: 74  DVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 133
           DV VELSESARAGVKASSDWVMESMNKGT+SYGVTTGFGATSHRRTKQGGALQKELIRFL
Sbjct: 69  DVTVELSESARAGVKASSDWVMESMNKGTESYGVTTGFGATSHRRTKQGGALQKELIRFL 128

Query: 134 NAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 193
           NAGIFG+GAES+HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL
Sbjct: 129 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 188

Query: 194 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGL 253
           RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGE +DAKEAFQQAGIPSGFFELQPKEGL
Sbjct: 189 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 248

Query: 254 ALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 313
           ALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE
Sbjct: 249 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 308

Query: 314 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 373
           AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER
Sbjct: 309 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 368

Query: 374 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 433
           EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN
Sbjct: 369 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 428

Query: 434 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 493
           NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 429 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 488

Query: 494 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGAL 553
           SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQ+AKKVLTT SNGAL
Sbjct: 489 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 548

Query: 554 HPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIF 613
           HPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEHALANG+ EKN DTSIF
Sbjct: 549 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 608

Query: 614 QKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGE 673
           QKI+ FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+YRFVREEL TK+LTGE
Sbjct: 609 QKIITFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 668

Query: 674 KVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           KVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 669 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 708

BLAST of Bhi03G001224 vs. NCBI nr
Match: XP_023518835.1 (phenylalanine ammonia-lyase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 667/716 (93.16%), Postives = 691/716 (96.51%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGG 60
           MAP  FQQ   QNGS+E+ CIS NDPLNWGVAAD+LKGSHLDEVK M+EEYRRP+VK+GG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCISHNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           E+LTISQVAAIATRD+DVIVEL+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVIVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFG+GAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQ 240
           AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGE +DA EAFQQ
Sbjct: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEH 600
           SQ AKKVL T SNGALHPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLITSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPI 660
           AL NGD EK+ADTSIFQKI  FEEELKA+LPKEVENARL YE+GNSKIENQIKDCRSYP+
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLWYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           YRFVREEL T +LTGEKVISPGEECEKVF ALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLLTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Bhi03G001224 vs. ExPASy TrEMBL
Match: A0A0A0KF39 (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G445760 PE=3 SV=1)

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 676/713 (94.81%), Postives = 699/713 (98.04%), Query Frame = 0

Query: 1   MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESL 60
           MA  HFQQ+NGS+ESLCI  NDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GGE+L
Sbjct: 1   MAFAHFQQKNGSVESLCIGSNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETL 60

Query: 61  TISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120
           TISQVAAIATRD+DVIVELSES+RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK
Sbjct: 61  TISQVAAIATRDTDVIVELSESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120

Query: 121 QGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180
           QGGALQKELIRFLNAGIFG+GAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT
Sbjct: 121 QGGALQKELIRFLNAGIFGSGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180

Query: 181 KLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGI 240
           KLLN+NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGER+ AKEAFQQA I
Sbjct: 181 KLLNNNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERITAKEAFQQADI 240

Query: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300
           PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH
Sbjct: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300

Query: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360
           LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL
Sbjct: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360

Query: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420
           IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM
Sbjct: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420

Query: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480
           FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA
Sbjct: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480

Query: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQV 540
           EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVK+TVSQ+
Sbjct: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKTTVSQI 540

Query: 541 AKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALA 600
           AKKVLTT SNG LHPSRFCEKDLLKVVDREYTF+YIDDPC+ATYPLSQKLRQVLVEHALA
Sbjct: 541 AKKVLTTSSNGTLHPSRFCEKDLLKVVDREYTFSYIDDPCSATYPLSQKLRQVLVEHALA 600

Query: 601 NGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRF 660
           NGD+EK  DTSIFQKI  FEEELKA+LPKEVEN+RLAYE+G+SKIENQIK+CRSYP+YRF
Sbjct: 601 NGDNEKTVDTSIFQKIASFEEELKAVLPKEVENSRLAYESGSSKIENQIKNCRSYPLYRF 660

Query: 661 VREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           VREELETKMLTGEKVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 661 VREELETKMLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 713

BLAST of Bhi03G001224 vs. ExPASy TrEMBL
Match: A0A1L5JHV3 (Phenylalanine ammonia-lyase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 669/700 (95.57%), Postives = 690/700 (98.57%), Query Frame = 0

Query: 14  ESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDS 73
           +SLC S+NDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GGE+LTISQVAAIATRDS
Sbjct: 9   DSLCHSRNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 68

Query: 74  DVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 133
           DV VELSESARAGVKASSDWVMESMNKGT+SYGVTTGFGATSHRRTKQGGALQKELIRFL
Sbjct: 69  DVTVELSESARAGVKASSDWVMESMNKGTESYGVTTGFGATSHRRTKQGGALQKELIRFL 128

Query: 134 NAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 193
           NAGIFG+GAES+HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL
Sbjct: 129 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 188

Query: 194 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGL 253
           RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGE +DAKEAFQQAGIPSGFFELQPKEGL
Sbjct: 189 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 248

Query: 254 ALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 313
           ALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE
Sbjct: 249 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 308

Query: 314 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 373
           AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER
Sbjct: 309 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 368

Query: 374 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 433
           EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN
Sbjct: 369 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 428

Query: 434 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 493
           NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 429 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 488

Query: 494 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGAL 553
           SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQ+AKKVLTT SNGAL
Sbjct: 489 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 548

Query: 554 HPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIF 613
           HPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEHALANG+ EKN DTSIF
Sbjct: 549 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 608

Query: 614 QKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGE 673
           QKI+ FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+YRFVREEL TK+LTGE
Sbjct: 609 QKIITFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 668

Query: 674 KVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           KVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 669 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 708

BLAST of Bhi03G001224 vs. ExPASy TrEMBL
Match: A0A6J1HGG8 (Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463284 PE=3 SV=1)

HSP 1 Score: 1313.1 bits (3397), Expect = 0.0e+00
Identity = 661/716 (92.32%), Postives = 692/716 (96.65%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGG 60
           MAP  FQQ   QNGS+E+ CI++NDPLNWGVAAD+LKGSHLDEVK M+EEYRRP+VK+GG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCITRNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           E+LTISQVAAIATRD+DV+V+L+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVLVQLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFG+GAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQ 240
           AITKLLN NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGE +DA EAFQQ
Sbjct: 181 AITKLLNSNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEH 600
           SQ AKKVLTT SNGALHPSRFCEKDLLKVVDRE+TFAYIDDPC+ATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLTTSSNGALHPSRFCEKDLLKVVDREHTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPI 660
           AL NGD EK+ADTSIFQKI  FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           YRFVREEL T ++TGEKVISPGEECEKVF ALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLVTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Bhi03G001224 vs. ExPASy TrEMBL
Match: A0A6J1HG90 (Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463286 PE=3 SV=1)

HSP 1 Score: 1312.7 bits (3396), Expect = 0.0e+00
Identity = 661/716 (92.32%), Postives = 692/716 (96.65%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGG 60
           MAP  FQQ   QNGS+E+ CI++NDPLNWGVAAD+LKGSHLDEVK M+EEYRRP+VK+GG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCITRNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           E+LTISQVAAIATRD+DV+V+L+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVLVQLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFG+GAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQ 240
           AITKLLN NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGE +DA EAFQQ
Sbjct: 181 AITKLLNTNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEH 600
           SQ AKKVLTT SNGALHPSRFCEKDLLKVVDRE+TFAYIDDPC+ATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLTTSSNGALHPSRFCEKDLLKVVDREHTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPI 660
           AL NGD EK+ADTSIFQKI  FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           YRFVREEL T ++TGEKVISPGEECEKVF ALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLVTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Bhi03G001224 vs. ExPASy TrEMBL
Match: A0A6J1HEN5 (Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463285 PE=3 SV=1)

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 661/716 (92.32%), Postives = 692/716 (96.65%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGG 60
           MAP  FQQ   QNGS+E+ CI++NDPLNWGVAAD+LKGSHLDEVK M+EEYRRP+VK+GG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCITRNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           E+LTISQVAAIATRD+DV+V+L+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVLVQLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFG+GAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQ 240
           AITKLLN NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+G NGE +DAKEAFQQ
Sbjct: 181 AITKLLNSNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGQNGESLDAKEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEH 600
           SQ AKKVLTT SNGALHPSRFCEKDLLKVVDRE+TFAYIDDPC+ATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLTTSSNGALHPSRFCEKDLLKVVDREHTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPI 660
           AL NGD EK+ADTSIFQKI  FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           YRFVREEL T ++TGEKVISPGEECEKVF ALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLVTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716