Bhi03G000614 (gene) Wax gourd (B227) v1

BLAST of Bhi03G000614 vs. TAIR 10
Match: AT3G51325.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 2.7e-12
Identity = 31/78 (39.74%), Postives = 48/78 (61.54%), Query Frame = 0

Query: 55  GAAMETEWCSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDED 114
           G   E E CSVCL ++  ++  + LPC HE H +CV+ WF +  ++  CP+CRF      
Sbjct: 18  GEGREEECCSVCLMRMEAKDVIKSLPCSHEFHSLCVDTWF-NVSRKICCPLCRF------ 77

Query: 115 VQTTEVLTEEMVIWFTCF 133
             TT +LT+E+++WF+ F
Sbjct: 78  SPTTILLTDELLLWFSSF 88

BLAST of Bhi03G000614 vs. TAIR 10
Match: AT1G15100.1 (RING-H2 finger A2A )

HSP 1 Score: 55.8 bits (133), Expect = 3.1e-08
Identity = 26/52 (50.00%), Postives = 32/52 (61.54%), Query Frame = 0

Query: 63  CSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDED 115
           C VCLSK+ E EE R L C+H  HK C+E W H  Q   TCP+CR  +  +D
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLH--QFNFTCPLCRSALVSDD 135

BLAST of Bhi03G000614 vs. TAIR 10
Match: AT2G42360.1 (RING/U-box superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.2e-07
Identity = 27/62 (43.55%), Postives = 35/62 (56.45%), Query Frame = 0

Query: 47  TSTHPTLDGAAMETEWCSVCLSKVMEREEARVLP-CQHEVHKICVERWFHDCQKQKTCPI 106
           T T    DG A     C+VCLS + E+++AR LP C+H  H  CV+ W   C    TCP+
Sbjct: 92  TFTVGATDGVAASATECAVCLSVLKEQDKARELPNCKHIFHVDCVDTWLTTC---STCPV 150

Query: 107 CR 108
           CR
Sbjct: 152 CR 150

BLAST of Bhi03G000614 vs. TAIR 10
Match: AT3G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 1.5e-07
Identity = 30/87 (34.48%), Postives = 43/87 (49.43%), Query Frame = 0

Query: 40  AKEKGCETSTHPTLDGA-----AMETE-WCSVCLSKVMEREEARVLPCQHEVHKICVERW 99
           AK+    +S    LD A       E E  CSVCL +V   E  R LPC H+ H  C++ W
Sbjct: 182 AKQASTSSSAEKMLDSANESKKGTEDELTCSVCLEQVTVGEIVRTLPCLHQFHAGCIDPW 241

Query: 100 FHDCQKQKTCPICRF-----WMRDEDV 116
               ++Q TCP+C+F     W   +++
Sbjct: 242 L---RQQGTCPVCKFRAHSGWQEQDEI 265

BLAST of Bhi03G000614 vs. TAIR 10
Match: AT2G47560.1 (RING/U-box superfamily protein )

HSP 1 Score: 53.1 bits (126), Expect = 2.0e-07
Identity = 24/50 (48.00%), Postives = 32/50 (64.00%), Query Frame = 0

Query: 59  ETEWCSVCLSKVMEREEARVLP-CQHEVHKICVERWFHDCQKQKTCPICR 108
           E E CSVCLS+  E +E R+LP C H  H  C++ WF   + + TCP+CR
Sbjct: 104 EKEECSVCLSEFEEEDEGRLLPKCGHSFHVDCIDTWF---RSRSTCPLCR 150

BLAST of Bhi03G000614 vs. ExPASy Swiss-Prot
Match: Q9ZT50 (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 4.3e-07
Identity = 26/52 (50.00%), Postives = 32/52 (61.54%), Query Frame = 0

Query: 63  CSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDED 115
           C VCLSK+ E EE R L C+H  HK C+E W H  Q   TCP+CR  +  +D
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLH--QFNFTCPLCRSALVSDD 135

BLAST of Bhi03G000614 vs. ExPASy Swiss-Prot
Match: Q5SPX3 (RING finger protein 215 OS=Mus musculus OX=10090 GN=Rnf215 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 4.3e-07
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = 0

Query: 60  TEWCSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRF 109
           TE C+VCL     ++  RVLPC+HE H+ CV+ W      Q+TCP+C+F
Sbjct: 324 TETCAVCLDYFCNKQWLRVLPCKHEFHRDCVDPWL---MLQQTCPLCKF 369

BLAST of Bhi03G000614 vs. ExPASy Swiss-Prot
Match: Q68DV7 (E3 ubiquitin-protein ligase RNF43 OS=Homo sapiens OX=9606 GN=RNF43 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 9.6e-07
Identity = 28/84 (33.33%), Postives = 43/84 (51.19%), Query Frame = 0

Query: 33  ASCFMQRAK--EKGCETSTHPTLDGAAMETEWCSVCLSKVMEREEARVLPCQHEVHKICV 92
           ASC   R +  + G   S+ P           C++CL +  E +E RV+ C HE H+ CV
Sbjct: 250 ASCRQARGEWPDSGSSCSSAPV----------CAICLEEFSEGQELRVISCLHEFHRNCV 309

Query: 93  ERWFHDCQKQKTCPICRFWMRDED 115
           + W H   + +TCP+C F + + D
Sbjct: 310 DPWLH---QHRTCPLCMFNITEGD 320

BLAST of Bhi03G000614 vs. ExPASy Swiss-Prot
Match: A5WWA0 (E3 ubiquitin-protein ligase znrf3 OS=Danio rerio OX=7955 GN=znrf3 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 9.6e-07
Identity = 30/90 (33.33%), Postives = 49/90 (54.44%), Query Frame = 0

Query: 25  ALLCVDSIASCFMQRAKEKGCETSTHPTLDG-AAMETEWCSVCLSKVMEREEARVLPCQH 84
           A+  ++ + +C   +AK KG   ++    D  ++  T  C++CL K ++ EE RV+PC H
Sbjct: 229 AIQALEKMETCKF-KAKFKGQREASCGASDSVSSSSTSDCAICLEKYIDGEELRVIPCAH 288

Query: 85  EVHKICVERWFHDCQKQKTCPICRFWMRDE 114
             HK CV+ W     +  TCP CR  + D+
Sbjct: 289 RFHKKCVDPWL---LQHHTCPHCRHNIIDQ 314

BLAST of Bhi03G000614 vs. ExPASy Swiss-Prot
Match: Q5NCP0 (E3 ubiquitin-protein ligase RNF43 OS=Mus musculus OX=10090 GN=Rnf43 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.3e-06
Identity = 24/72 (33.33%), Postives = 38/72 (52.78%), Query Frame = 0

Query: 39  RAKEKGCE--TSTHPTLDGAAMETEWCSVCLSKVMEREEARVLPCQHEVHKICVERWFHD 98
           R  + GC    +  P    +   T  C++CL +  E +E RV+ C HE H+ CV+ W + 
Sbjct: 246 RRYQAGCRRARAEWPDSGSSCSSTPVCAICLEEFSEGQELRVISCLHEFHRTCVDPWLY- 305

Query: 99  CQKQKTCPICRF 109
             + +TCP+C F
Sbjct: 306 --QHRTCPLCMF 314

BLAST of Bhi03G000614 vs. NCBI nr
Match: XP_038883680.1 (E3 ubiquitin-protein ligase znrf3-like [Benincasa hispida])

HSP 1 Score: 283.1 bits (723), Expect = 1.3e-72
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0

Query: 2   VCRKIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMETE 61
           + +KIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMETE
Sbjct: 40  ILQKIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMETE 99

Query: 62  WCSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDEDVQTTEVL 121
           WCSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDEDVQTTEVL
Sbjct: 100 WCSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDEDVQTTEVL 159

Query: 122 TEEMVIWFTCFQVI 136
           TEEMVIWFTCFQVI
Sbjct: 160 TEEMVIWFTCFQVI 173

BLAST of Bhi03G000614 vs. NCBI nr
Match: KAG6603894.1 (hypothetical protein SDJN03_04503, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 195.7 bits (496), Expect = 2.7e-46
Identity = 89/117 (76.07%), Postives = 97/117 (82.91%), Query Frame = 0

Query: 21  GIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMETEWCSVCLSKVMEREEARVLP 80
           GIK+AL C D I SCFM+RAKE+GCETS HPTLD AAME EWCSVCLS VMER+ A++LP
Sbjct: 76  GIKDALSCGDVIDSCFMKRAKEEGCETSAHPTLDDAAMEREWCSVCLSNVMERKVAQILP 135

Query: 81  CQHEVHKICVERWFHDCQKQKTCPICRFWMRDEDVQTTEVLTEEMVIWFTCFQVIGY 138
           CQHE HKICVERWFH+CQ QKTC ICRFW+ DED Q TEVLTEEMVIW T   VI Y
Sbjct: 136 CQHEFHKICVERWFHECQNQKTCSICRFWVSDEDCQPTEVLTEEMVIWSTSLHVIDY 192

BLAST of Bhi03G000614 vs. NCBI nr
Match: XP_022132660.1 (E3 ubiquitin-protein ligase RNF181 homolog [Momordica charantia])

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-44
Identity = 94/139 (67.63%), Postives = 109/139 (78.42%), Query Frame = 0

Query: 1   MVCRKIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMET 60
           MV RKIISS +NF+ L+  PGIK+AL C D I S   QRAK++GCE   HPTL   A+  
Sbjct: 1   MVYRKIISSFYNFVVLKLYPGIKDALFCGDVIESHVKQRAKKEGCEALAHPTLYD-AVGR 60

Query: 61  EWCSVCLSKVMEREEARV--LPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDEDVQTT 120
           EWCSVCLSKVME EE+RV  LPC HE HKIC++RWFH+C  QKTCPIC+FW+RD+D QTT
Sbjct: 61  EWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHEC--QKTCPICQFWLRDDDGQTT 120

Query: 121 EVLTEEMVIWFTCFQVIGY 138
           EVLTEEMVIWFT F V+GY
Sbjct: 121 EVLTEEMVIWFTSFHVVGY 136

BLAST of Bhi03G000614 vs. NCBI nr
Match: XP_022977421.1 (uncharacterized protein LOC111477769 isoform X1 [Cucurbita maxima])

HSP 1 Score: 181.4 bits (459), Expect = 5.2e-42
Identity = 81/108 (75.00%), Postives = 90/108 (83.33%), Query Frame = 0

Query: 30  DSIASCFMQRAKEKGCETSTHPTLDGAAMETEWCSVCLSKVMEREEARVLPCQHEVHKIC 89
           D I S FM+RAKE+GCETS HPTLD AA+E EWCSVC S VMER+ A++LPCQHE HKIC
Sbjct: 62  DVIDSYFMKRAKEEGCETSAHPTLDDAAIEREWCSVCFSNVMERKVAQILPCQHEFHKIC 121

Query: 90  VERWFHDCQKQKTCPICRFWMRDEDVQTTEVLTEEMVIWFTCFQVIGY 138
           VERWFH+CQ QKTCPIC+FW+RDED Q TEVLTEEMVIW T   VI Y
Sbjct: 122 VERWFHECQNQKTCPICQFWVRDEDCQPTEVLTEEMVIWSTSLHVIDY 169

BLAST of Bhi03G000614 vs. NCBI nr
Match: XP_031276738.1 (E3 ubiquitin-protein ligase At1g12760 [Pistacia vera])

HSP 1 Score: 109.4 bits (272), Expect = 2.5e-20
Identity = 67/142 (47.18%), Postives = 87/142 (61.27%), Query Frame = 0

Query: 1   MVCRKIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAM-- 60
           MV +K+ISS +N I  R  P  K A    + IAS   ++A E G  +S+  +L GA++  
Sbjct: 1   MVLKKLISSIYNLIIPRGVPRSK-AAPSYEVIASGVREKA-EDGASSSS--SLAGASLAE 60

Query: 61  --ETEWCSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWMR-DEDV 120
             E E C VCLS + E E+ RVLPC HE H+ C+ RWF+ C   KTCPICRF M+ DE+ 
Sbjct: 61  DSECELCCVCLSGLKEGEDCRVLPCLHEFHRACINRWFNAC--SKTCPICRFSMKEDENF 120

Query: 121 QTTEVLTEEMVIWFTCFQVIGY 138
              E  TEEMVIWF+ F V G+
Sbjct: 121 HFREEFTEEMVIWFSSFHVAGF 136

BLAST of Bhi03G000614 vs. ExPASy TrEMBL
Match: A0A6J1BT35 (E3 ubiquitin-protein ligase RNF181 homolog OS=Momordica charantia OX=3673 GN=LOC111005465 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.4e-45
Identity = 94/139 (67.63%), Postives = 109/139 (78.42%), Query Frame = 0

Query: 1   MVCRKIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMET 60
           MV RKIISS +NF+ L+  PGIK+AL C D I S   QRAK++GCE   HPTL   A+  
Sbjct: 1   MVYRKIISSFYNFVVLKLYPGIKDALFCGDVIESHVKQRAKKEGCEALAHPTLYD-AVGR 60

Query: 61  EWCSVCLSKVMEREEARV--LPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDEDVQTT 120
           EWCSVCLSKVME EE+RV  LPC HE HKIC++RWFH+C  QKTCPIC+FW+RD+D QTT
Sbjct: 61  EWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHEC--QKTCPICQFWLRDDDGQTT 120

Query: 121 EVLTEEMVIWFTCFQVIGY 138
           EVLTEEMVIWFT F V+GY
Sbjct: 121 EVLTEEMVIWFTSFHVVGY 136

BLAST of Bhi03G000614 vs. ExPASy TrEMBL
Match: A0A6J1IJV3 (uncharacterized protein LOC111477769 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477769 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.5e-42
Identity = 81/108 (75.00%), Postives = 90/108 (83.33%), Query Frame = 0

Query: 30  DSIASCFMQRAKEKGCETSTHPTLDGAAMETEWCSVCLSKVMEREEARVLPCQHEVHKIC 89
           D I S FM+RAKE+GCETS HPTLD AA+E EWCSVC S VMER+ A++LPCQHE HKIC
Sbjct: 62  DVIDSYFMKRAKEEGCETSAHPTLDDAAIEREWCSVCFSNVMERKVAQILPCQHEFHKIC 121

Query: 90  VERWFHDCQKQKTCPICRFWMRDEDVQTTEVLTEEMVIWFTCFQVIGY 138
           VERWFH+CQ QKTCPIC+FW+RDED Q TEVLTEEMVIW T   VI Y
Sbjct: 122 VERWFHECQNQKTCPICQFWVRDEDCQPTEVLTEEMVIWSTSLHVIDY 169

BLAST of Bhi03G000614 vs. ExPASy TrEMBL
Match: A0A5B6YSP6 (Putative E3 ubiquitin-protein ligase RHA2A (Fragment) OS=Davidia involucrata OX=16924 GN=Din_004126 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.0e-19
Identity = 62/139 (44.60%), Postives = 85/139 (61.15%), Query Frame = 0

Query: 1   MVCRKIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMET 60
           MV +K++S   +FI+LR    IK++    + I S    + KE G +      +   A+E 
Sbjct: 10  MVIKKLVSFICSFISLRGRRRIKDS-SSYEVIESNVADKVKE-GPDEPPGRLVRSEAIEV 69

Query: 61  EWCSVCLSKVME-REEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDED-VQTT 120
           E C VCLS++ E  E+ RVLPC HE H++CV+RWF+ C   KTCP+CRF M DE+  Q  
Sbjct: 70  ELCCVCLSRLKEGGEDTRVLPCLHEFHRVCVDRWFNVC--SKTCPVCRFLMEDEEKFQKR 129

Query: 121 EVLTEEMVIWFTCFQVIGY 138
           E LTEEMVIWF+ F   G+
Sbjct: 130 EDLTEEMVIWFSSFHAAGF 144

BLAST of Bhi03G000614 vs. ExPASy TrEMBL
Match: A0A5C7HSR0 (RING-type domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_014412 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.3e-19
Identity = 62/141 (43.97%), Postives = 83/141 (58.87%), Query Frame = 0

Query: 1   MVCRKIISSCFNFIALRSCP-GIKNALLCVDSIASCFMQRAKEKGCETSTHP--TLDGAA 60
           MV ++++ S +NFI L   P G K ALL  D  AS        +G   S  P  +   A 
Sbjct: 1   MVFKRLVFSLYNFITLGGQPLGTKAALLSYDVTASSI-----NEGNSNSISPGGSFVDAN 60

Query: 61  METEWCSVCLSKVMEREEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWM-RDEDVQ 120
            E+E C VCLS++ + E+ RVLPC H+ HK+CV+ WF+ C   KTCP+CRF M  +E   
Sbjct: 61  FESESCCVCLSRLEDGEDMRVLPCLHKFHKLCVDMWFNAC--TKTCPLCRFSMVEEEKFL 120

Query: 121 TTEVLTEEMVIWFTCFQVIGY 138
             E LTEEM+IWF+ F V G+
Sbjct: 121 RKEELTEEMIIWFSSFHVAGF 134

BLAST of Bhi03G000614 vs. ExPASy TrEMBL
Match: F6H5I9 (RING-type domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_00s0349g00040 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.9e-19
Identity = 58/140 (41.43%), Postives = 83/140 (59.29%), Query Frame = 0

Query: 1   MVCRKIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMET 60
           ++ +K+++   N I LRS   IK A     ++     ++AK    E      L+G   E 
Sbjct: 2   VMVKKLLAFILNLIGLRSRQSIKEASTYEVAVGLGSQEKAKGGSAEVK----LEGEGDEC 61

Query: 61  EWCSVCLSKVME--REEARVLPCQHEVHKICVERWFHDCQKQKTCPICRFWM-RDEDVQT 120
           E CS+CLS+  E   + ARVLPC H+ HK C+++WF+ C  +KTCP+CRF M  +E+ Q 
Sbjct: 62  ELCSICLSRYREGIGQGARVLPCMHKFHKACIDKWFNMC--RKTCPVCRFSMGAEENFQK 121

Query: 121 TEVLTEEMVIWFTCFQVIGY 138
            E LTEEMVIWF+ F V G+
Sbjct: 122 REELTEEMVIWFSSFHVAGF 135