Bhi03G000364 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000364
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionAspergillus nuclease S(1)
Locationchr3: 9530387 .. 9535029 (+)
RNA-Seq ExpressionBhi03G000364
SyntenyBhi03G000364
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCTAACGTTCGTAACCTCTGATTGGGTGAGAGGCAAGCGTCGAAAAACGAGAAGCGAAGCTGGTTTAATTTTGCAGACAAGCTTCAGAGCCGGCGGTCAAGTAGGTGAAGTCGGAGCTGCTATCGCTGCGAAGAGTTGATTTCTAGTTCCATTGTGTTCACTATCTTATTTTGTTGTTAGGAGCAGACATGGGCCAGTCTCAACTTTGCTGGACTGCGAATGCTTTCGTTTTTCTGCTACTTTTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGTAATTTGCAAGATAGCGGAGGTAAGCATATTCGAGTCATTTTAAGCATCAATTGCGTTTAATTTTGTCCAGCTTTTCGGTGTAATGAAAGGTTAATACGGAAGGATTATAATTGATGGGATTTGGATCTTGAGTTCTTGAAAGTGTTCCTCTCGTAGTTGTGATACCATAGGCCAGGATTCAATTTTAAACAAACATGGCAAAGGAGAGAGGGGTCCTGTGAGATTTGGTTTGCTATTAGAAAAACATTTGGACCCATTTGGATTTTTTGCATAAAAACTGAAAAAATTGAGCATCTCAGTAAGCTAGAACTTTCTTGCGGTCAGTTATTTTGCAGCTGATTATGACATATCACTCTATGATTATGATTTCAACCATTATATCCTGTACAAATTTTGTTGAAGCCAAAATCTGATATCACTTCCTTTGGTTTAGGGATATTTCACTGAAGATACTCTATCCACGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTTGCGGCTGTATGCTCCTGGCCTGATGAAATTCAACGAAAAGCTCATTATCGCTGGAGTAGCGCCTTACACTACGTTGACACGCCCGATTTCTTTTGTAATTATAATTGCTCGAGTAAGCTTAAGCTTTATGGTATTTTCAATTTCTCTTGTAGTCTATCATGCCGTATAACTCTTTGATGTAATGCTTGTTGTCATTGTGTTCATGAGCTTTAAAATCAAATTCATTGTTGAACCAGAAGAACACGTCTGAGACTGAGAAATTTCATAGTTCATGTCCTCTAATGTTATATGGTTAATGTCACCATGATCTATCTGATTTGGATATATAGTACTGTAAAATCAATGTCTTCCTACTCTAAGATTTTGACTGAAACAAACTCATTCTGCAATTCGGTTGAGTTTAATGAAAGTGTGTCATTTTGGATATGTCATATATGACTGTTATTAAATTCAACTGGGTTTTGATTCCATTCGTTCAACAAGGTACCAATATTTAGCTCTAATAACTAATTTTCAATACTTGAAACTTGAAAGTTATATTTTGATGATGTTTGCTTGTGTAACAGTATTCTCTGCTTCTTGATAGGAGACTGTCATGACACTCATGGACATAAGGGTAGATGTGTGACTGCAGCAATTTACAACTATACTATGCAACTCGAATCAGCTTACAAAGAGATGACTTCAGAAATTAGATGTAAGCATCTTAGATGACTAATCTTGTGTCTATACACTTTTTGAACAAATGTCATTTTTATTCACATGATATTTTCAGATAACTTAACAGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGATGTCCATCAGGTTTGCTTCTACAGATTTACACTAAAAGGCAGAGATAAATGAAAAATGTGAAGTGCTAGATTCATGAAATTGGTGTAACTGCAAAATTTAGTGCTCTGAATGTTGTTGAAAGTTCATTTTCATCAATGGGCTGTTCATGCCCCTCATCCTTTTTAAATAAGATTTCTTAAGCAGGTTTGTGTATTGATTACATGCATACATTATAGCCTCCATTAATGGACTTTCACCTTTTCCTTTTACAGCACTAAAATTCCATAATATAATGCTTTTTGAAATTCTTCAATGGTGCTGGTTGCTTGTGCCTGGATATTGTTCTTTGCACCCTTAAATAGATGCAAAATTTACCCCTAACCACATATTACTAATAATAATGTCTCCTTTATTTTATTTTTCGAAAAAGAGTGGGATAATCAATGATTTTATCTCTTTGCATCTGACTTTACCGTTATTCAATGGCAGCCTCTGCATGTCGGTTTTGTTGGAGATCTAGGAGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTCCATCATGTAAGTGTCACCTAGAGATTTCAAATCCCTCCCACACCACGAGTACCCTGCCAAGCTTGGAAATCCCATGACATTCTGCAGAATGTTGTGGTTTTTTATTCATCTGGTATTGGAATAGTTTTGTCTATAAAAAATAAATAGTTTTGCTTTAGGTCTGGGATAACATGATCATCGAGTCTGCCTTGAAGACATTCTACCATTCAAACCTTACGCTAATGATTCAAGCGATTCAAAACAACATTTCGGTATTATATGCCTCCTCTTTCTACTTTTATGTCCTTGTGTACCATTTACGATAGGAGTGGAACAGCTTGTTTTTTGTAGTTTTTACTTGTAGTTATTTTTCCTTATGGCATCATCTTTTTAAATTTCTGAAGGACGAATGGTATAATGATGTCTCAGCTTGGAGAAATTGCACACTGAACCACACAGCTTGTCCTAACCCGTGAGTGTTTATATTCTTCTCCCTTATCCATGTCTGGTGAGATGCTAATGTTAAATCATATATTTGAATTGTTCCTCTGCTATGATTCGAACATCTCTGATCAGTATACGAAAGTGAGATGATGCTTGTGATAAAGATGGACTTGCCTTGTTATGTCTTCAAGACTCCAAGCAGTGGAGTATAACATGTTGCTCAAGACTTATGATGTCCTTTTCTGAATTTGGAAGGGGAGAAAAACATTTAGAGGATGAATAGTAACCTTCATTTGCCTACCATAAAGTTCTAGGCATAACAAAAATTTATTGTGGTGCCTCTTAATTTTCAGGTATGCTTCTGAAAGTATTAGCTTGGCATGTAAATATGCATACAAGAACGCCACTCCAGGAAGCACACTAGAAGGTATGGTCACAATCACGTTAACTTTTCAATGCTGTGGTAATAAGTAGGTGGGATCTGATCTTTTTAATGGGGATTGGTGGAGGTGGGTATGAGCATAATGCAGAATTTGAGACCTTGATATCCTGGTGATAAGAAAGAAAGAAAAGGGTGTAGCCCTATGGGAATTGGTTCTATATGTCAAAATCGGTTAGGTGGTACTTGGTTTGTTGTTGCACTTGCAATCCTTCCTTGCCATAAAAAGATACACATTTGCCTGTCATAAAGATCAGATGAGTTGATTGTGTTAAATCTTCCAGTTTCTATATTCTGCTCGAGAAATTTTAAAACCCATCTCTCTATCCGTAGGAATGTCTCCAATACAAGTACATTCGTAAATCTTCCTTTCTGTTCTTTCAATATGATGCAAAAATCTGAACTCTTAATCTGTTAATAGTTGCTCCTGCTCAAAAGACATTCCAGAAATTCAACTCTTCAGTTTTTTCTTCATTGTCGAGGCATATGAACATGCTAGAATAGTTCGTTTTTTTCTCTGTGCTCTTTACACCTTGGATCTATAGAATTTAGAGTCAGTGGTGCACAAATAAGCAAAAAAATTCCTGTTTGTTGCAGACAGTTATTTTCTTAGCCGGTTGCCAGTCGTCGAGAAGAGGTTAGCACAAAGTGGCATAAGATTGGCTTCTACCCTCAACCGTATCTTTGCTTCTGAAGTGAAAGTGGCTGAAGTTTGAAGACAATGAGCGGGAGAAATCAGATCAACTTCAATAAGATATTAAAATGGTTGAAGAACATGAATTGATTTATGGTATTTGCTATTACTCCAAAAGAACCCTAAATGAGGAGAGTCAGTTGTAACTAGAGCAGTCACTGTAAAAGAACATGATATTCAGGAATGATATGATTGTTGCTTTACTGCTTTTAGAGATCAAAAAGCTCAAGATTGCAATTAAGGCAAATTTCTCATATGTTAAGAATCTGTCCTTAAATTTTAAACAATATCTGTCTACAGGCCAAGTAGAGCATCGTTGAGTTCAACAATATGAAATTGTTTGAGGCCGATACAACTTGACTACTTCAAAAGCATCTTGAATTTGTATCTTACATTGAATAGTGCAAAATTGTTAACCACATTGTTTCCTCGTTATTTCTTGTCGGGTGCTCATTTACTTTTGTGCATTGCAGCTTCTACTTAAAACAAGAGTGCAAGTATGGTTCATTTTGAAGCTCTAAGAGTGTTGTCACTTCAAGACAAACCCATACCATCACACATTCAATCTAGAAACCCAGTTTTATGAGAGATTCATAGGAGATAACAAAGTGTCAAACTCAAAGATCTGAGTCTAACATCCTGG

mRNA sequence

ATTCTAACGTTCGTAACCTCTGATTGGGTGAGAGGCAAGCGTCGAAAAACGAGAAGCGAAGCTGGTTTAATTTTGCAGACAAGCTTCAGAGCCGGCGGTCAAGAGCAGACATGGGCCAGTCTCAACTTTGCTGGACTGCGAATGCTTTCGTTTTTCTGCTACTTTTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGTAATTTGCAAGATAGCGGAGGGATATTTCACTGAAGATACTCTATCCACGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTTGCGGCTGTATGCTCCTGGCCTGATGAAATTCAACGAAAAGCTCATTATCGCTGGAGTAGCGCCTTACACTACGTTGACACGCCCGATTTCTTTTGTAATTATAATTGCTCGAGAGACTGTCATGACACTCATGGACATAAGGGTAGATGTGTGACTGCAGCAATTTACAACTATACTATGCAACTCGAATCAGCTTACAAAGAGATGACTTCAGAAATTAGATATAACTTAACAGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGATGTCCATCAGCCTCTGCATGTCGGTTTTGTTGGAGATCTAGGAGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTCCATCATGTCTGGGATAACATGATCATCGAGTCTGCCTTGAAGACATTCTACCATTCAAACCTTACGCTAATGATTCAAGCGATTCAAAACAACATTTCGGACGAATGGTATAATGATGTCTCAGCTTGGAGAAATTGCACACTGAACCACACAGCTTGTCCTAACCCGTATGCTTCTGAAAGTATTAGCTTGGCATGTAAATATGCATACAAGAACGCCACTCCAGGAAGCACACTAGAAGACAGTTATTTTCTTAGCCGGTTGCCAGTCGTCGAGAAGAGGTTAGCACAAAGTGGCATAAGATTGGCTTCTACCCTCAACCGTATCTTTGCTTCTGAAGTGAAAGTGGCTGAAGTTTGAAGACAATGAGCGGGAGAAATCAGATCAACTTCAATAAGATATTAAAATGGTTGAAGAACATGAATTGATTTATGGTATTTGCTATTACTCCAAAAGAACCCTAAATGAGGAGAGTCAGTTGTAACTAGAGCAGTCACTGTAAAAGAACATGATATTCAGGAATGATATGATTGTTGCTTTACTGCTTTTAGAGATCAAAAAGCTCAAGATTGCAATTAAGCTTCTACTTAAAACAAGAGTGCAAGTATGGTTCATTTTGAAGCTCTAAGAGTGTTGTCACTTCAAGACAAACCCATACCATCACACATTCAATCTAGAAACCCAGTTTTATGAGAGATTCATAGGAGATAACAAAGTGTCAAACTCAAAGATCTGAGTCTAACATCCTGG

Coding sequence (CDS)

ATGGGCCAGTCTCAACTTTGCTGGACTGCGAATGCTTTCGTTTTTCTGCTACTTTTACCTGGAATCCTCGGCTGGGGAAAGGAAGGTCACTATGTAATTTGCAAGATAGCGGAGGGATATTTCACTGAAGATACTCTATCCACGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTTGCGGCTGTATGCTCCTGGCCTGATGAAATTCAACGAAAAGCTCATTATCGCTGGAGTAGCGCCTTACACTACGTTGACACGCCCGATTTCTTTTGTAATTATAATTGCTCGAGAGACTGTCATGACACTCATGGACATAAGGGTAGATGTGTGACTGCAGCAATTTACAACTATACTATGCAACTCGAATCAGCTTACAAAGAGATGACTTCAGAAATTAGATATAACTTAACAGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGATGTCCATCAGCCTCTGCATGTCGGTTTTGTTGGAGATCTAGGAGGTAATTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTCCATCATGTCTGGGATAACATGATCATCGAGTCTGCCTTGAAGACATTCTACCATTCAAACCTTACGCTAATGATTCAAGCGATTCAAAACAACATTTCGGACGAATGGTATAATGATGTCTCAGCTTGGAGAAATTGCACACTGAACCACACAGCTTGTCCTAACCCGTATGCTTCTGAAAGTATTAGCTTGGCATGTAAATATGCATACAAGAACGCCACTCCAGGAAGCACACTAGAAGACAGTTATTTTCTTAGCCGGTTGCCAGTCGTCGAGAAGAGGTTAGCACAAAGTGGCATAAGATTGGCTTCTACCCTCAACCGTATCTTTGCTTCTGAAGTGAAAGTGGCTGAAGTTTGA

Protein sequence

MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGDLAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASESISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV
Homology
BLAST of Bhi03G000364 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 445.3 bits (1144), Expect = 3.9e-125
Identity = 205/297 (69.02%), Postives = 243/297 (81.82%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           M  S   W A   V   L+ G L WGKEGHY +CKIAE YF E+T++ VK+LLP SA+GD
Sbjct: 1   MSSSLRQWFARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LA+VCSWPDEI+    +RW+S LHYVDTPD+ CNY   RDCHDTH ++ RCVT AI+NYT
Sbjct: 61  LASVCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
           MQL SA +   + + YNLTEALMFLSHFIGD+HQPLHVGF+GD GGN+ITVRWYRRKTNL
Sbjct: 121 MQLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKT+Y+ +L LMI+A+Q N++++W NDV  W +C LN TACPNPYASE
Sbjct: 181 HHVWDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVA 298
           SI+LACKYAY+NATPG+TL D YFLSRLP+VEKRLAQ GIRLA+TLNRIF+S+ K A
Sbjct: 241 SINLACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSSKPKHA 297

BLAST of Bhi03G000364 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 399.1 bits (1024), Expect = 3.2e-111
Identity = 181/291 (62.20%), Postives = 230/291 (79.04%), Query Frame = 0

Query: 8   WTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAE-GDLAAVCS 67
           W  +  V   L+ G L WGK+GHY +CK+AEG+F +DT++ VK+LLP S + G LA  CS
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCS 63

Query: 68  WPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYTMQLESA 127
           WPDEI++ + ++W+S LHYV+TP++ CNY   RDCHDTH HK  CVT AI+NYT QL SA
Sbjct: 64  WPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSA 123

Query: 128 YKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNLHHVWDN 187
            +   + + YNLTEAL+FLSH++GDVHQPLH GF+GDLGGN+I V WY  K+NLHHVWDN
Sbjct: 124 SENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWDN 183

Query: 188 MIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASESISLAC 247
           MII+SAL+T+Y+S+L  MIQA+Q  + + W NDV +W++C  +  ACPN YASESI LAC
Sbjct: 184 MIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDLAC 243

Query: 248 KYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVA 298
           KYAY+NATPG+TL D YFLSRLPVVEKRLAQ GIRLA+TLNRIF+++ K+A
Sbjct: 244 KYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSAKPKLA 294

BLAST of Bhi03G000364 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 390.6 bits (1002), Expect = 1.2e-108
Identity = 184/297 (61.95%), Postives = 223/297 (75.08%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MG S   W  +  V   L+ G L WG  GHY +CKIA+ YF EDT+  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI++   +RW+SALH+ DTPD+ CNY  SRDC      K  CVT AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
            QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GDLGGN I V WY ++TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           H VWD+MIIESAL+T+Y+S+L  MI  +Q  + + W NDV +W +C LN TACPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVA 298
           SI LACKYAY+NAT G+TL D YF+SRLPVVEKRLAQ GIRLA TLNRIF+++ K+A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLA 292

BLAST of Bhi03G000364 vs. TAIR 10
Match: AT4G21590.2 (endonuclease 3 )

HSP 1 Score: 390.6 bits (1002), Expect = 1.2e-108
Identity = 184/297 (61.95%), Postives = 223/297 (75.08%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MG S   W  +  V   L+ G L WG  GHY +CKIA+ YF EDT+  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI++   +RW+SALH+ DTPD+ CNY  SRDC      K  CVT AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
            QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GDLGGN I V WY ++TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           H VWD+MIIESAL+T+Y+S+L  MI  +Q  + + W NDV +W +C LN TACPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVA 298
           SI LACKYAY+NAT G+TL D YF+SRLPVVEKRLAQ GIRLA TLNRIF+++ K+A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLA 292

BLAST of Bhi03G000364 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 327.4 bits (838), Expect = 1.2e-89
Identity = 157/275 (57.09%), Postives = 191/275 (69.45%), Query Frame = 0

Query: 16  LLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGDLAAVCSWPDEIQRKA 75
           L   P I GWGKEGH +ICKIA+    E     VKELLP SAEGDL+++C W D +  K 
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRV--KF 78

Query: 76  HYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYTMQLESAYKEMTSEIR 135
            Y WSS LHY++TPD  C+Y  +RDC D  G KGRCV  AIYNYT QL S     +S+ +
Sbjct: 79  RYHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQ 138

Query: 136 YNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKT 195
           YNLTEAL+F+SHF+GD+HQPLHV +  D GGN+I V WY RK NLHH+WD+ IIE+A   
Sbjct: 139 YNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNIIETAEAD 198

Query: 196 FYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASESISLACKYAYKNATP 255
            Y+S L  M+ A++ NI+ EW + V  W  CT   TACP+ YASE I  AC +AYK  T 
Sbjct: 199 LYNSALEGMVDALKKNITTEWADQVKRWETCT-KKTACPDIYASEGIQAACDWAYKGVTE 258

Query: 256 GSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIF 291
           G TLED YF SRLP+V +RLAQ G+RLA+TLNRIF
Sbjct: 259 GDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

BLAST of Bhi03G000364 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 5.5e-124
Identity = 205/297 (69.02%), Postives = 243/297 (81.82%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           M  S   W A   V   L+ G L WGKEGHY +CKIAE YF E+T++ VK+LLP SA+GD
Sbjct: 1   MSSSLRQWFARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LA+VCSWPDEI+    +RW+S LHYVDTPD+ CNY   RDCHDTH ++ RCVT AI+NYT
Sbjct: 61  LASVCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
           MQL SA +   + + YNLTEALMFLSHFIGD+HQPLHVGF+GD GGN+ITVRWYRRKTNL
Sbjct: 121 MQLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKT+Y+ +L LMI+A+Q N++++W NDV  W +C LN TACPNPYASE
Sbjct: 181 HHVWDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVA 298
           SI+LACKYAY+NATPG+TL D YFLSRLP+VEKRLAQ GIRLA+TLNRIF+S+ K A
Sbjct: 241 SINLACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSSKPKHA 297

BLAST of Bhi03G000364 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 4.6e-110
Identity = 181/291 (62.20%), Postives = 230/291 (79.04%), Query Frame = 0

Query: 8   WTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAE-GDLAAVCS 67
           W  +  V   L+ G L WGK+GHY +CK+AEG+F +DT++ VK+LLP S + G LA  CS
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCS 63

Query: 68  WPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYTMQLESA 127
           WPDEI++ + ++W+S LHYV+TP++ CNY   RDCHDTH HK  CVT AI+NYT QL SA
Sbjct: 64  WPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSA 123

Query: 128 YKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNLHHVWDN 187
            +   + + YNLTEAL+FLSH++GDVHQPLH GF+GDLGGN+I V WY  K+NLHHVWDN
Sbjct: 124 SENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWDN 183

Query: 188 MIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASESISLAC 247
           MII+SAL+T+Y+S+L  MIQA+Q  + + W NDV +W++C  +  ACPN YASESI LAC
Sbjct: 184 MIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDLAC 243

Query: 248 KYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVA 298
           KYAY+NATPG+TL D YFLSRLPVVEKRLAQ GIRLA+TLNRIF+++ K+A
Sbjct: 244 KYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSAKPKLA 294

BLAST of Bhi03G000364 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 1.6e-107
Identity = 184/297 (61.95%), Postives = 223/297 (75.08%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MG S   W  +  V   L+ G L WG  GHY +CKIA+ YF EDT+  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI++   +RW+SALH+ DTPD+ CNY  SRDC      K  CVT AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
            QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GDLGGN I V WY ++TNL
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           H VWD+MIIESAL+T+Y+S+L  MI  +Q  + + W NDV +W +C LN TACPNPYASE
Sbjct: 181 HRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVA 298
           SI LACKYAY+NAT G+TL D YF+SRLPVVEKRLAQ GIRLA TLNRIF+++ K+A
Sbjct: 241 SIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKRKLA 292

BLAST of Bhi03G000364 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.7e-88
Identity = 157/275 (57.09%), Postives = 191/275 (69.45%), Query Frame = 0

Query: 16  LLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGDLAAVCSWPDEIQRKA 75
           L   P I GWGKEGH +ICKIA+    E     VKELLP SAEGDL+++C W D +  K 
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRV--KF 78

Query: 76  HYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYTMQLESAYKEMTSEIR 135
            Y WSS LHY++TPD  C+Y  +RDC D  G KGRCV  AIYNYT QL S     +S+ +
Sbjct: 79  RYHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQ 138

Query: 136 YNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKT 195
           YNLTEAL+F+SHF+GD+HQPLHV +  D GGN+I V WY RK NLHH+WD+ IIE+A   
Sbjct: 139 YNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNIIETAEAD 198

Query: 196 FYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASESISLACKYAYKNATP 255
            Y+S L  M+ A++ NI+ EW + V  W  CT   TACP+ YASE I  AC +AYK  T 
Sbjct: 199 LYNSALEGMVDALKKNITTEWADQVKRWETCT-KKTACPDIYASEGIQAACDWAYKGVTE 258

Query: 256 GSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIF 291
           G TLED YF SRLP+V +RLAQ G+RLA+TLNRIF
Sbjct: 259 GDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

BLAST of Bhi03G000364 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 9.6e-84
Identity = 146/287 (50.87%), Postives = 196/287 (68.29%), Query Frame = 0

Query: 14  VFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGDLAAVCSWPDEIQR 73
           + L  +  +  W KEGH + C+IA+          V+ LLP   +GDL+A+C WPD+I+ 
Sbjct: 18  LILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCVWPDQIRH 77

Query: 74  KAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYTMQLESAYKEMTSE 133
              YRW+S LHY+DTPD  C+Y  SRDCHD HG K  CV  AI N+T QL+  Y E TS+
Sbjct: 78  WYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQH-YGEGTSD 137

Query: 134 IRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNLHHVWDNMIIESAL 193
            RYN+TEAL+FLSHF+GD+HQP+HVGF  D GGN+I +RWY+ K+NLHHVWD  II +AL
Sbjct: 138 RRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDREIILTAL 197

Query: 194 KTFYHSNLTLMIQAIQNNISDE-WYNDVSAWRNCTLNHTACPNPYASESISLACKYAYKN 253
           K  Y  NL L+ + ++ NI++  W++D+S+W  C  +  ACP+ YASESI LACK+ YK 
Sbjct: 198 KENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECN-DLIACPHKYASESIKLACKWGYKG 257

Query: 254 ATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
              G TL + YF +RLP+V KR+ Q G+RLA  LNR+F+ +  +A V
Sbjct: 258 VKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDDHAIAGV 302

BLAST of Bhi03G000364 vs. NCBI nr
Match: XP_038883252.1 (endonuclease 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 629.4 bits (1622), Expect = 1.6e-176
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
           MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL
Sbjct: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
           SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 299

BLAST of Bhi03G000364 vs. NCBI nr
Match: XP_038883248.1 (endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883250.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883251.1 endonuclease 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 620.5 bits (1599), Expect = 7.3e-174
Identity = 298/305 (97.70%), Postives = 298/305 (97.70%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSR------DCHDTHGHKGRCVTA 120
           LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCS       DCHDTHGHKGRCVTA
Sbjct: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSSKLKLYGDCHDTHGHKGRCVTA 120

Query: 121 AIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180
           AIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY
Sbjct: 121 AIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACP 240
           RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEV 300
           NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEV
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEV 300

BLAST of Bhi03G000364 vs. NCBI nr
Match: XP_023543506.1 (endonuclease 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 593.6 bits (1529), Expect = 9.6e-166
Identity = 282/299 (94.31%), Postives = 288/299 (96.32%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQS+LC  A AF FLLL+PGILGWGKEGHY+ICKIAEGYFTEDTLS VKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI+ KAHYRWSSALHYVDTPDFFCNYN SRDCHDTHGHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
            QLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKTFYHSNLTLMIQAIQ NIS EWYNDVSAWRNC LNHTACPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
           SISLACKYAYKNATPGSTLEDSYFLSRLP+VEKRLAQSGIRLASTLNRIFASEVKVAE+
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAEI 299

BLAST of Bhi03G000364 vs. NCBI nr
Match: XP_022978904.1 (endonuclease 4-like [Cucurbita maxima])

HSP 1 Score: 590.5 bits (1521), Expect = 8.1e-165
Identity = 281/299 (93.98%), Postives = 287/299 (95.99%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQS+LC  A AF FLLL+PGILGWGKEGHY+ICKIAEGYFTEDTLS VKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI+ KAHYRWSSALHYVDTPDFFCNYN SRDCHDTH HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHEHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
            QLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKTFYHSNLTLMIQAIQ NIS EWYNDVSAWRNC LNHTACPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
           SISLACKYAYKNATPGSTLEDSYFLSRLP+VEKRLAQSGIRLASTLNRIFASEVKVAE+
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAEI 299

BLAST of Bhi03G000364 vs. NCBI nr
Match: XP_022950165.1 (endonuclease 4-like [Cucurbita moschata])

HSP 1 Score: 588.2 bits (1515), Expect = 4.0e-164
Identity = 279/299 (93.31%), Postives = 286/299 (95.65%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQ +LC  A AF FLLL+PGILGWGKEGHY+ICKIAEGYFTEDTLS VKELLPASAEGD
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI+ KAHYRWSSALHYVDTPDFFCNYN SRDCHDTHGHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
            QLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKTFYHSNLTLMIQAIQ NIS EWYNDVSAWRNC LNHTACPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
           SI LACKYAY+NATPGSTLEDSYFLSRLP+VEKRLAQSGIRLASTLNRIFASEVKVAE+
Sbjct: 241 SIRLACKYAYRNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAEI 299

BLAST of Bhi03G000364 vs. ExPASy TrEMBL
Match: A0A6J1IVA4 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 3.9e-165
Identity = 281/299 (93.98%), Postives = 287/299 (95.99%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQS+LC  A AF FLLL+PGILGWGKEGHY+ICKIAEGYFTEDTLS VKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI+ KAHYRWSSALHYVDTPDFFCNYN SRDCHDTH HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHEHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
            QLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKTFYHSNLTLMIQAIQ NIS EWYNDVSAWRNC LNHTACPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
           SISLACKYAYKNATPGSTLEDSYFLSRLP+VEKRLAQSGIRLASTLNRIFASEVKVAE+
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAEI 299

BLAST of Bhi03G000364 vs. ExPASy TrEMBL
Match: A0A6J1GEX6 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.0e-164
Identity = 279/299 (93.31%), Postives = 286/299 (95.65%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQ +LC  A AF FLLL+PGILGWGKEGHY+ICKIAEGYFTEDTLS VKELLPASAEGD
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI+ KAHYRWSSALHYVDTPDFFCNYN SRDCHDTHGHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
            QLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKTFYHSNLTLMIQAIQ NIS EWYNDVSAWRNC LNHTACPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
           SI LACKYAY+NATPGSTLEDSYFLSRLP+VEKRLAQSGIRLASTLNRIFASEVKVAE+
Sbjct: 241 SIRLACKYAYRNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEVKVAEI 299

BLAST of Bhi03G000364 vs. ExPASy TrEMBL
Match: A0A1S3CEU3 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.5e-156
Identity = 263/299 (87.96%), Postives = 278/299 (92.98%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQS+LCWTAN FVFLLLLPGILGWG+EGHY +CKIAEGY TED LS V+ELLPASAEGD
Sbjct: 21  MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 80

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSW DE++    Y WSS LH+VDTPDFFCNYNCSRDCHD+H HKGRCVTAAIYNYT
Sbjct: 81  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 140

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
           MQLESAYKE+TSE+RYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGN I V WY+R TNL
Sbjct: 141 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWYQRSTNL 200

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWD MIIESALK FYHSNL LMIQAIQ+NISDEW+N+VSAWRNCT NHTACPNPYASE
Sbjct: 201 HHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACPNPYASE 260

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
           SISLACKYAYKNATPGSTLEDSYFL+RLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV
Sbjct: 261 SISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 319

BLAST of Bhi03G000364 vs. ExPASy TrEMBL
Match: A0A1S3CF84 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.5e-156
Identity = 263/299 (87.96%), Postives = 278/299 (92.98%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MGQS+LCWTAN FVFLLLLPGILGWG+EGHY +CKIAEGY TED LS V+ELLPASAEGD
Sbjct: 1   MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSW DE++    Y WSS LH+VDTPDFFCNYNCSRDCHD+H HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
           MQLESAYKE+TSE+RYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGN I V WY+R TNL
Sbjct: 121 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWYQRSTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWD MIIESALK FYHSNL LMIQAIQ+NISDEW+N+VSAWRNCT NHTACPNPYASE
Sbjct: 181 HHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 300
           SISLACKYAYKNATPGSTLEDSYFL+RLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV
Sbjct: 241 SISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEVKVAEV 299

BLAST of Bhi03G000364 vs. ExPASy TrEMBL
Match: A0A6J1CLU4 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111012332 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 5.7e-156
Identity = 265/298 (88.93%), Postives = 279/298 (93.62%), Query Frame = 0

Query: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAEGYFTEDTLSTVKELLPASAEGD 60
           MG+ +L W A+  VFLLL+PGILGWGKEGHY ICKIAEGYFTED LS VKELLPASAEGD
Sbjct: 1   MGRFELFWRAS--VFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI+RKAHY WSS LHYVDTPDFFCNYN SRDCHDTHGH GRCV AAIYNYT
Sbjct: 61  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 120

Query: 121 MQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWYRRKTNL 180
           MQLES YKEMTS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWYRRKTNL
Sbjct: 121 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWYRRKTNL 180

Query: 181 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACPNPYASE 240
           HHVWDNMIIESALKTFY+S+L LMIQAIQN+I DEWYNDVS+WRNCT NHT CPNPYASE
Sbjct: 181 HHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCPNPYASE 240

Query: 241 SISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEVKVAE 299
           SISLACKYAYKNATPGSTL+DSYFLSRLPVVEKRLAQSGIRLA+TLNRI ASEVKVAE
Sbjct: 241 SISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEVKVAE 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G21585.13.9e-12569.02endonuclease 4 [more]
AT4G21600.13.2e-11162.20endonuclease 5 [more]
AT4G21590.11.2e-10861.95endonuclease 3 [more]
AT4G21590.21.2e-10861.95endonuclease 3 [more]
AT1G68290.11.2e-8957.09endonuclease 2 [more]
Match NameE-valueIdentityDescription
F4JJL05.5e-12469.02Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1[more]
F4JJL34.6e-11062.20Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1[more]
Q8LDW61.6e-10761.95Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1[more]
Q9C9G41.7e-8857.09Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1[more]
Q9SXA69.6e-8450.87Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038883252.11.6e-176100.00endonuclease 4-like isoform X2 [Benincasa hispida][more]
XP_038883248.17.3e-17497.70endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease ... [more]
XP_023543506.19.6e-16694.31endonuclease 4-like [Cucurbita pepo subsp. pepo][more]
XP_022978904.18.1e-16593.98endonuclease 4-like [Cucurbita maxima][more]
XP_022950165.14.0e-16493.31endonuclease 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1IVA43.9e-16593.98Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1[more]
A0A6J1GEX62.0e-16493.31Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=... [more]
A0A1S3CEU31.5e-15687.96Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1[more]
A0A1S3CF841.5e-15687.96Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1[more]
A0A6J1CLU45.7e-15688.93Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111012332 PE=3 SV... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008947Phospholipase C/P1 nuclease domain superfamilyGENE3D1.10.575.10P1 Nucleasecoord: 25..295
e-value: 1.3E-101
score: 341.9
IPR008947Phospholipase C/P1 nuclease domain superfamilySUPERFAMILY48537Phospholipase C/P1 nucleasecoord: 25..289
IPR003154S1/P1 nucleasePFAMPF02265S1-P1_nucleasecoord: 25..290
e-value: 6.8E-80
score: 268.6
IPR003154S1/P1 nucleasePANTHERPTHR33146ENDONUCLEASE 4coord: 5..297
IPR003154S1/P1 nucleaseCDDcd11010S1-P1_nucleasecoord: 25..290
e-value: 3.8881E-84
score: 251.535
NoneNo IPR availablePANTHERPTHR33146:SF23ENDONUCLEASE 5coord: 5..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000364Bhi03M000364mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006308 DNA catabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004521 endoribonuclease activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000014 single-stranded DNA endodeoxyribonuclease activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds