Bhi03G000339 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000339
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionEpoxide hydrolase-like
Locationchr3: 8781751 .. 8784363 (-)
RNA-Seq ExpressionBhi03G000339
SyntenyBhi03G000339
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTGATGAAAGCTTATCTTTGGACTGGACAAACACAAAAAAACAAACTGTACTGTACTGGATGTGATTGTATTCAAAAGGGCTTGATTATTGTTGCTTCAATTGAAAGAATTCTCACCTTCTTTTATTTCTTTCACCCCTTTTGTGAATCAAGAAGCTCTGGATGGATAAAATTCAGCACGTATTCGTCGAAGTTCAAGGAGGATTGGAAATCCATGTAGCGGAGATCGGAACCGGTGATCAATTTCTCCGCCGGAGCGGAACTTTCTTTTCCTTTTACAACAAAAACATTTTCTCAGTTCCGCCGATGTTTGTTTGTGTCTTTCAGGTTCAAATGTCGTGGTTTTTCTTCACGGTTTTCCGGAGATTTGGTACTCATGGCGCCACCAGATGATTGCTGTGGCTGACGCCGGATTCCGAGCAATTGCTGTCGATTACAGAGGATATGGACTCTCTGATCCGCCGCACGAGCCCTCCAAGGCTACTTACAGCGATCTCATTTCCGACCTCCTTGAAATCCTCGACACCCTCGATATCCCCAAGGTACTAACCCTTCATTCAAATTCAAACCCATGTCCTGTAATTTTAACCAGAGCTGTAGAAATCCGTTAGAATGTTAGATGGAAGTTGAGACTTGAAACGTGAGATTTTTGTGTGGATAATCTAAATTTAGTTTTGCTAATGTCGAATGGCTCGAATTCTAGAAATAATGATATGTAACATTTTGCTTATGCCACAAAGTGTGTCAACGATAAATTGTCCATCCGCAATCGCAGGTCTCATCCATCGTGAATTGAAGAGACCTTGAACGTGATTTCACATTTGAGAAAAATCTAAGATCGCAAAATTTTATGAATATTGCCTTTCGGGTCTTGCGATGGATGCGAACTCGCGATCCCAAATGGACTTCAAAGAGAATTTAGTGGTGGTTGGTGTGTCGTAAGTAAAAGTTTAGTTTTCCTTGTTTTTTCGAATCTAGGTCATTTGACATTAATGCAACCAAATTTAAGTCATCTGTTGTGTAGTGATGACCTTGAAAGAAAATTGTGACCGTTACACCGTTTTAGGACGCCACTACATCATTCTGGCTTATGTTTTTGTGGAATCTGGTTTAAAAATAAGAATGTTCTAATAGTATTAGTGGGAAATTGGGAATAGGTGTTTCTTGTGGGGAAAGATTTTGGAGCAATGCCAGCTTATTACTTTGCTATCAAACACCCAGAGAGGGCATTAGGAGTTGTGACATTGGGAGTGCCATTTATGCCTCCTAAAAGACCTAACTTCCAAGACCACCTCCCTGAAGGCTTCTACATTTCCAGATGGCAGGTATTAAACTCAAACATGTACAAATCTTTACTATGATCATTTTGGTTGTTTTAAAGTTCCTTTTGTATGGTTTTCTTGTATAGGAACCTGGACGAGCCGAGGCTGATTTTAGTCGTTTCGATGCAAAAACCGTTGTTAGGAATGTCTATATCCTTTTCTCTAGAAGTGAAATCCCAATAGCACAAGAAAATCAGGAGATAATGGACTTGGTGGATTCATCTACTCCTCTTCCCCCTTGGTTTACTGAGGAAGATCTTGCAGCTTATGGAGAGCTCTATGAGAAATCTGGATTTCACACTGCATTAAAAGTTCCTTACAGGTAATGTAATGTCTTTGGTTGAAATACCTTGAATTTGTTATCTGCCTTGAAACCTAAATTGTATTCGTGTTTATGTTCTTTACATTATTTACCTTCAGGTTTAAAGTTCGCTTTATAAACTCTCTAACTCTACTAACATCTTTAAATCTAAAATATTATATTCTCTCTTTATGTTCGTGGATGTAGCTAATACACAAGTGAACCACGTAAATTTCTGTGTTGATTCTCTATTGTTTATCTTTATTTGTCGATTTCATAACACCCTTGAATCTTGTTTATGGAGTTTTATTCTAGCTTCACTGAGTTTCTGAACTGGGATTGATGAATTATACATTATAGGTCAATGAATGAAGATTGGGGAGTAAAAGATCCAAAAGTGGAAATTCCAGCTCTTCTTGTAATGGGTGAAAAAGATTATGTTCTAAAATTTCCAGGAATTGAGGAGTATGTAAGAAGTGAAAAAGTGAAAGATTATGTTCCAAAATTGGAGATCATCTTTCTACCAGAAGGATCTCATTTTGTTCAAGAGCAATCTCCAGAAGAAGTTAATCACCTGCTGCTCAGTTTCCTTGCCAAGCATACATGATTTGGTTTGGACATCCGAATACAGAGGTTAAATCTAAGTAAACCTTTTACTTATTGAGTACTTTTGTTGTTTTTGAATTCCAAAACCATGGACAGCATATGTATTATTGTATTTGATAAGTTAGATGTGGGTATAATCCATAGATGTCAATGCCTTGTACTAATGAAGTGCTCAACAGGGCAATTTCCTTGATAAAAACATTTCAAATGTTGAGAGCCAAAAACATATCTAACTACCATTTTAGAAATGGACTTTCATTTCAAATTTTTTGGGCAGAGAGTGCTTAACTTTCCTTCTGATACTTTTTCACCAAGAGCAAATGACTCATTTGCATTGTCTAATAATCTAACGTTGAATTCACCAACAGATTCATCGTCATGCG

mRNA sequence

CTTTGATGAAAGCTTATCTTTGGACTGGACAAACACAAAAAAACAAACTGTACTGTACTGGATGTGATTGTATTCAAAAGGGCTTGATTATTGTTGCTTCAATTGAAAGAATTCTCACCTTCTTTTATTTCTTTCACCCCTTTTGTGAATCAAGAAGCTCTGGATGGATAAAATTCAGCACGTATTCGTCGAAGTTCAAGGAGGATTGGAAATCCATGTAGCGGAGATCGGAACCGGTTCAAATGTCGTGGTTTTTCTTCACGGTTTTCCGGAGATTTGGTACTCATGGCGCCACCAGATGATTGCTGTGGCTGACGCCGGATTCCGAGCAATTGCTGTCGATTACAGAGGATATGGACTCTCTGATCCGCCGCACGAGCCCTCCAAGGCTACTTACAGCGATCTCATTTCCGACCTCCTTGAAATCCTCGACACCCTCGATATCCCCAAGGTGTTTCTTGTGGGGAAAGATTTTGGAGCAATGCCAGCTTATTACTTTGCTATCAAACACCCAGAGAGGGCATTAGGAGTTGTGACATTGGGAGTGCCATTTATGCCTCCTAAAAGACCTAACTTCCAAGACCACCTCCCTGAAGGCTTCTACATTTCCAGATGGCAGGAACCTGGACGAGCCGAGGCTGATTTTAGTCGTTTCGATGCAAAAACCGTTGTTAGGAATGTCTATATCCTTTTCTCTAGAAGTGAAATCCCAATAGCACAAGAAAATCAGGAGATAATGGACTTGGTGGATTCATCTACTCCTCTTCCCCCTTGGTTTACTGAGGAAGATCTTGCAGCTTATGGAGAGCTCTATGAGAAATCTGGATTTCACACTGCATTAAAAGTTCCTTACAGGTCAATGAATGAAGATTGGGGAGTAAAAGATCCAAAAGTGGAAATTCCAGCTCTTCTTGTAATGGGTGAAAAAGATTATGTTCTAAAATTTCCAGGAATTGAGGAGTATGTAAGAAGTGAAAAAGTGAAAGATTATGTTCCAAAATTGGAGATCATCTTTCTACCAGAAGGATCTCATTTTGTTCAAGAGCAATCTCCAGAAGAAGTTAATCACCTGCTGCTCAGTTTCCTTGCCAAGCATACATGATTTGGTTTGGACATCCGAATACAGAGGTTAAATCTAAGTAAACCTTTTACTTATTGAGTACTTTTGTTGTTTTTGAATTCCAAAACCATGGACAGCATATGTATTATTGTATTTGATAAGTTAGATGTGGGTATAATCCATAGATGTCAATGCCTTGTACTAATGAAGTGCTCAACAGGGCAATTTCCTTGATAAAAACATTTCAAATGTTGAGAGCCAAAAACATATCTAACTACCATTTTAGAAATGGACTTTCATTTCAAATTTTTTGGGCAGAGAGTGCTTAACTTTCCTTCTGATACTTTTTCACCAAGAGCAAATGACTCATTTGCATTGTCTAATAATCTAACGTTGAATTCACCAACAGATTCATCGTCATGCG

Coding sequence (CDS)

ATGGATAAAATTCAGCACGTATTCGTCGAAGTTCAAGGAGGATTGGAAATCCATGTAGCGGAGATCGGAACCGGTTCAAATGTCGTGGTTTTTCTTCACGGTTTTCCGGAGATTTGGTACTCATGGCGCCACCAGATGATTGCTGTGGCTGACGCCGGATTCCGAGCAATTGCTGTCGATTACAGAGGATATGGACTCTCTGATCCGCCGCACGAGCCCTCCAAGGCTACTTACAGCGATCTCATTTCCGACCTCCTTGAAATCCTCGACACCCTCGATATCCCCAAGGTGTTTCTTGTGGGGAAAGATTTTGGAGCAATGCCAGCTTATTACTTTGCTATCAAACACCCAGAGAGGGCATTAGGAGTTGTGACATTGGGAGTGCCATTTATGCCTCCTAAAAGACCTAACTTCCAAGACCACCTCCCTGAAGGCTTCTACATTTCCAGATGGCAGGAACCTGGACGAGCCGAGGCTGATTTTAGTCGTTTCGATGCAAAAACCGTTGTTAGGAATGTCTATATCCTTTTCTCTAGAAGTGAAATCCCAATAGCACAAGAAAATCAGGAGATAATGGACTTGGTGGATTCATCTACTCCTCTTCCCCCTTGGTTTACTGAGGAAGATCTTGCAGCTTATGGAGAGCTCTATGAGAAATCTGGATTTCACACTGCATTAAAAGTTCCTTACAGGTCAATGAATGAAGATTGGGGAGTAAAAGATCCAAAAGTGGAAATTCCAGCTCTTCTTGTAATGGGTGAAAAAGATTATGTTCTAAAATTTCCAGGAATTGAGGAGTATGTAAGAAGTGAAAAAGTGAAAGATTATGTTCCAAAATTGGAGATCATCTTTCTACCAGAAGGATCTCATTTTGTTCAAGAGCAATCTCCAGAAGAAGTTAATCACCTGCTGCTCAGTTTCCTTGCCAAGCATACATGA

Protein sequence

MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERALGVVTLGVPFMPPKRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRSEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGVKDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEEVNHLLLSFLAKHT
Homology
BLAST of Bhi03G000339 vs. TAIR 10
Match: AT3G51000.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 216.5 bits (550), Expect = 3.1e-56
Identity = 115/309 (37.22%), Postives = 186/309 (60.19%), Query Frame = 0

Query: 14  GLEIHVAEIGTGSN-VVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRGYGLSD--PP 73
           G+ ++VAE G     +V+ LHGFPE WYSWRHQ+  ++  G+  +A D RGYG SD  P 
Sbjct: 14  GIWLNVAEKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVVAPDLRGYGDSDSLPS 73

Query: 74  HEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERALGVVTLGVPF 133
           HE    T S L++D++ +LD     + F+ G D+GA+  +   +  P+R  G ++L VP+
Sbjct: 74  HE--SYTVSHLVADVIGLLDHYGTTQAFVAGHDWGAIIGWCLCLFRPDRVKGFISLSVPY 133

Query: 134 MPP----KRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRSEIPIAQ 193
            P     K  +F     +G YI+++Q+PGRAEA F++ D  +V++  ++L +R++  +A 
Sbjct: 134 FPRDPKLKPSDFFKIFGDGLYITQFQKPGRAEAAFAKHDCLSVMKK-FLLITRTDYLVAP 193

Query: 194 ENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGV----KDP 253
            + EI+D ++  + +P W TEE++  Y E +++SGF   L   YRSM+ +W +    +D 
Sbjct: 194 PDTEIIDHLEIPSTIPDWITEEEIQVYAEKFQRSGFTGPLNY-YRSMDMNWEILAPWQDS 253

Query: 254 KVEIPALLVMGEKDYVLKFP-GIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEEVN 311
           K+ +P   + G+KD   + P G  EYV+ E  K  VP LEI+ +  G HF+Q++  E+V+
Sbjct: 254 KIVVPTKFIAGDKDIGYEGPNGTMEYVKGEVFKIVVPNLEIVVIEGGHHFIQQEKSEQVS 313

BLAST of Bhi03G000339 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 214.9 bits (546), Expect = 9.0e-56
Identity = 112/321 (34.89%), Postives = 181/321 (56.39%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           M+KI+H  +    G+ +HVA IG+G  V++F+HGFP++WYSWRHQ+++ A  G+RAIA D
Sbjct: 1   MEKIEHTTIST-NGINMHVASIGSGP-VILFVHGFPDLWYSWRHQLVSFAALGYRAIAPD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
            RGYG SD P      T   ++ DL+ +LD+L + +VFLVG D+GA+ A++  +  P+R 
Sbjct: 61  LRGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRV 120

Query: 121 LGVVTLGVPFMPPKRPN------FQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVY 180
             +V   V F  P+ P+      F+    + +YI R+QEPG  E DF++ D K ++   +
Sbjct: 121 NALVNTSVVF-NPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFF 180

Query: 181 ILFSRSEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMN 240
              SR+  P           +     LP W TE+D+  YG+ + + GF   L   YR++N
Sbjct: 181 T--SRNPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNY-YRALN 240

Query: 241 EDWGVKDP----KVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEG-S 300
             W +  P    ++++P   ++G+ D     PG +EY+    +K +VP L+ + + EG  
Sbjct: 241 LSWELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVG 300

Query: 301 HFVQEQSPEEVNHLLLSFLAK 311
           HF+ ++ P+EV   +  F  K
Sbjct: 301 HFLHQEKPDEVTDHIYGFFKK 315

BLAST of Bhi03G000339 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 199.9 bits (507), Expect = 3.0e-51
Identity = 112/314 (35.67%), Postives = 179/314 (57.01%), Query Frame = 0

Query: 14  GLEIHVAEIGTGSN-VVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRGYGLSDPPHE 73
           G++IHVA  G     +V+ LHGFPE+WYSWRHQ+  +A  G+RA+A D RGYG SD P E
Sbjct: 10  GIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRGYGDSDAPAE 69

Query: 74  PSKATYSDLISDLLEILDTL---DIPKVFLVGKDFGAMPAYYFAIKHPERALGVVTLGVP 133
            S  T  +++ DL+ ++  L   +  KVF+VG D+GA+ A+Y  +  P+R   +V L VP
Sbjct: 70  ISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRVKALVNLSVP 129

Query: 134 FM-----PPKRP--NFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRSEI 193
           F      P  +P    +    + +YI R+QE G  EA+ +    + V++   +L  R+  
Sbjct: 130 FSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKR--LLTYRTPG 189

Query: 194 P-IAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGVKD 253
           P I  +++        + PLP W TEED+A +   +E+ GF   +   YR+ N +  +  
Sbjct: 190 PVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNY-YRNFNRNNELLG 249

Query: 254 P----KVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEG-SHFVQEQS 311
           P    K+++P   V+GE D V   PG++EY+   + K+ VP LE   + EG +HF+ ++ 
Sbjct: 250 PWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAHFINQEK 309

BLAST of Bhi03G000339 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 1.9e-50
Identity = 112/325 (34.46%), Postives = 183/325 (56.31%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSN-VVVFLHGFPEIWYSWRHQMIAVADAGFRAIAV 60
           M+ I H  V V  G+ +H+AE G     VV+ LHGFP++WY+WRHQ+  ++  G+RA+A 
Sbjct: 1   MEGIDHRMVSV-NGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAP 60

Query: 61  DYRGYGLSDPPHEPSKATYSDLISDLLEILDTL--DIPKVFLVGKDFGAMPAYYFAIKHP 120
           D RGYG SD P   S+ T  +++ DL+ +LD++  +  KVFLVG D+GA+  ++  +  P
Sbjct: 61  DLRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRP 120

Query: 121 ERALGVVTLGVPFM---PPKRP--NFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRN 180
           E+  G V L VP+    P  +P   F+    + +YI R+QEPG+ E + +  D +  +RN
Sbjct: 121 EKINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRN 180

Query: 181 VYILFSRSEIPIAQENQ--EIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPY 240
           ++   +     + ++N   E  +    +  LP WF+++DL  Y   +EK+GF   L   Y
Sbjct: 181 LFTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNY-Y 240

Query: 241 RSMNEDWGVKDP----KVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKL-EIIFL 300
           R+M+ +W +  P    K+++P   + G+ D V   PG++EY+        VP L EI+ +
Sbjct: 241 RAMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVI 300

Query: 301 PEGSHFVQEQSPEEVNHLLLSFLAK 311
            +  HFV ++ P+EV   +  F  K
Sbjct: 301 EDAGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Bhi03G000339 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 8.2e-49
Identity = 108/312 (34.62%), Postives = 179/312 (57.37%), Query Frame = 0

Query: 14  GLEIHVAEIG-TGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRGYGLSDPPHE 73
           G++IHVA  G +   +V+ LHGFPE+WYSWRHQ+  +A  G+RA+A D RGYG SD P E
Sbjct: 10  GIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRGYGDSDAPAE 69

Query: 74  PSKATYSDLISDLLEILDTL--DIPKVFLVGKDFGAMPAYYFAIKHPERALGVVTLGVPF 133
            S  T  +++ DL+ ++ TL  +  KVF+VG D+GA+ A+Y  +  P++   +V L VP 
Sbjct: 70  ISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVKALVNLSVPL 129

Query: 134 M-----PPKRPNFQDHLPEG--FYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRSEIP 193
                 P  +P  +     G  +Y+ R+QE G  EA+ +    + V++   +L  R+  P
Sbjct: 130 SFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKR--LLTYRTPGP 189

Query: 194 -IAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGVKDP 253
            I  +++        + PLP W TEED+A +   +++ GF   +   YR+ N +  +  P
Sbjct: 190 LIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNY-YRNFNRNNELLGP 249

Query: 254 ----KVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEG-SHFVQEQSP 310
               K+++P   V+GE D V   PG++EY+   + K+ VP +E   + EG +HF+ ++ P
Sbjct: 250 WVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHFLNQEKP 309

BLAST of Bhi03G000339 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 7.5e-39
Identity = 108/319 (33.86%), Postives = 166/319 (52.04%), Query Frame = 0

Query: 4   IQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRG 63
           + H +V V+  + +H  E+G+G   V   HGFPE WYSWR+Q+ A+A AG+R +A+D +G
Sbjct: 237 MSHGYVTVKPRVRLHFVELGSGP-AVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKG 296

Query: 64  YGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERALGV 123
           YG S  P E  +     L  +++  LD L + +   +G D+G M  +Y A+ +PER   V
Sbjct: 297 YGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAV 356

Query: 124 VTLGVPFMPPKRPNFQ-----DHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFS 183
            +L  PF+ P  PN          P   Y   +QEPG AEA+      + + R    LF 
Sbjct: 357 ASLNTPFI-PANPNMSPLESIKANPVFDYQLYFQEPGVAEAELE----QNLSRTFKSLFR 416

Query: 184 RSEIPIAQENQ--EIMDLVDSSTPLPP---WFTEEDLAAYGELYEKSGFHTALKVPYRSM 243
            S+  +   ++  E   L  +S   P      TEE++  Y + ++KSGF   L   YR+M
Sbjct: 417 ASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNW-YRNM 476

Query: 244 --NEDWGVKD--PKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGS 303
             N  W  K    K+ IPAL+V  EKD+VL  P +     S+ ++D++P L+   + +  
Sbjct: 477 ERNWKWACKSLGRKILIPALMVTAEKDFVL-VPQM-----SQHMEDWIPHLKRGHIEDCG 536

Query: 304 HFVQEQSPEEVNHLLLSFL 309
           H+ Q   P EVN +L+ +L
Sbjct: 537 HWTQMDKPTEVNQILIKWL 542

BLAST of Bhi03G000339 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 1.4e-37
Identity = 102/320 (31.87%), Postives = 163/320 (50.94%), Query Frame = 0

Query: 4   IQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRG 63
           + H +V V+ G+ +H  E+G+G   +   HGFPE W+SWR+Q+ A+A AGFR +A+D +G
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGP-ALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKG 294

Query: 64  YGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERALGV 123
           YG S  P E  +     L  +++  LD L IP+   +G D+  +  +  A+ +PER   V
Sbjct: 295 YGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAV 354

Query: 124 VTLGVPFMPPKRPNFQD-----HLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFS 183
            +L  PFMPP  P+         +P   Y   +QEPG AEA+      K + R     F 
Sbjct: 355 ASLNTPFMPPD-PDVSPMKVIRSIPVFNYQLYFQEPGVAEAELE----KNMSRTFKSFFR 414

Query: 184 RSE------IPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRS 243
            S+      +  A E   I+        L    TEE++  Y + ++K+GF   L   YR+
Sbjct: 415 ASDETGFIAVHKATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNW-YRN 474

Query: 244 MNEDWGVK----DPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEG 303
              +W         K+ +PAL+V  EKD VL+ P +     S+ ++ ++P L+   + + 
Sbjct: 475 TERNWKWSCKGLGRKILVPALMVTAEKDIVLR-PEM-----SKNMEKWIPFLKRGHIEDC 534

Query: 304 SHFVQEQSPEEVNHLLLSFL 309
            H+ Q + P EVN +L+ +L
Sbjct: 535 GHWTQIEKPTEVNQILIKWL 541

BLAST of Bhi03G000339 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.8e-37
Identity = 101/320 (31.56%), Postives = 162/320 (50.62%), Query Frame = 0

Query: 4   IQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRG 63
           + H +V V+ G+ +H  E+G+G   +   HGFPE W+SWR+Q+ A+A AGFR +A+D +G
Sbjct: 235 VSHGYVTVKPGIRLHFVEMGSGP-AICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKG 294

Query: 64  YGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERALGV 123
           YG S  P E  +     L  +++  L+ L IP+   +G D+  +  +  A+ HPER   V
Sbjct: 295 YGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAV 354

Query: 124 VTLGVPFMPPKRPNFQD-----HLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFS 183
            +L  P MPP  P          +P   Y   +QEPG AEA+      K + R     F 
Sbjct: 355 ASLNTPLMPP-NPEVSPMEVIRSIPVFNYQLYFQEPGVAEAELE----KNMSRTFKSFFR 414

Query: 184 RSE------IPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRS 243
            S+      +  A E   I+        +    TEE++  Y + ++KSGF   L   YR+
Sbjct: 415 TSDDMGLLTVNKATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNW-YRN 474

Query: 244 MNEDW----GVKDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEG 303
              +W         K+ +PAL+V  EKD VL+ P +     S+ +++++P L+   + + 
Sbjct: 475 TERNWKWSCKALGRKILVPALMVTAEKDIVLR-PEM-----SKNMENWIPFLKRGHIEDC 534

Query: 304 SHFVQEQSPEEVNHLLLSFL 309
            H+ Q + P EVN +L+ +L
Sbjct: 535 GHWTQIEKPAEVNQILIKWL 541

BLAST of Bhi03G000339 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.4e-37
Identity = 105/320 (32.81%), Postives = 163/320 (50.94%), Query Frame = 0

Query: 4   IQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRG 63
           + H +V ++ G+ +H  E+G+G   V   HGFPE W+SWR+Q+ A+A AGFR +AVD +G
Sbjct: 237 LSHGYVLIKPGVRLHFVEMGSGP-AVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKG 296

Query: 64  YGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERALGV 123
           YG S  P E  + +   L  D++  L+ L + +   +G D+G +  +  A+ +PER   V
Sbjct: 297 YGESSAPPEIEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAV 356

Query: 124 VTLGVPFMPPKRPNFQ-----DHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFS 183
            +L  PFM P  PN          P   Y   +QEPG AEA+  +   +T     +  F 
Sbjct: 357 ASLNTPFM-PSNPNVSPMEIIKANPVFDYQLYFQEPGVAEAELEQNLDRT-----FKNFF 416

Query: 184 RSEIPIAQENQEIMDL--VDSSTPLPP----WFTEEDLAAYGELYEKSGFHTALKVPYRS 243
           R+          + +L  +   TP  P      TEED+  Y + ++KSGF   L   YR+
Sbjct: 417 RAHDETFLTTNRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNW-YRN 476

Query: 244 M--NEDWGVKDP--KVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEG 303
           M  N  WG K    K+ IPAL+V  E D VL          S+ +++++P L+   + + 
Sbjct: 477 MERNWQWGCKGSGRKILIPALMVTAENDLVL------HPKMSKHMENWIPHLKRGHIKDC 536

Query: 304 SHFVQEQSPEEVNHLLLSFL 309
            H+ Q   P E+N +L+ +L
Sbjct: 537 GHWTQIDKPAELNRILIEWL 542

BLAST of Bhi03G000339 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.9e-34
Identity = 98/312 (31.41%), Postives = 156/312 (50.00%), Query Frame = 0

Query: 14  GLEIHVAEIG-TGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVDYRGYGLSDPPHE 73
           G+ + V E G  G+ VV+  HGFPE+ YSWRHQ+ A+ADAG+  +A D RGYG S  P  
Sbjct: 13  GVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSSRPEA 72

Query: 74  PSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERALGVVTLGVPFMP 133
                   L +DL+ +LD +   +   VG D+GA+  +   + H +R   V  L VP +P
Sbjct: 73  IEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSVPALP 132

Query: 134 ----PKRPNFQDHLPEG-FYISRWQEPGRAEADFSRFDAKTVVRNVYIL-----FSRSEI 193
               P    F+    E  FYI  +QEPG A+A+ +   A+T+ R +  L      S +  
Sbjct: 133 RAQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQSAAMR 192

Query: 194 PIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDW----G 253
            +A      +D +     LP W ++E+L  Y   + ++GF   L   YR+ + +W     
Sbjct: 193 MLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNW-YRNFDRNWETTAD 252

Query: 254 VKDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYV--PKLEIIFLPEGSHFVQEQS 309
           +    + +P+L + G  D VL F       R+++  + +  P  E++ +    H++Q++ 
Sbjct: 253 LAGKTISVPSLFIAGTADPVLTF------TRTDRAAEVISGPYREVL-IDGAGHWLQQER 312

BLAST of Bhi03G000339 vs. NCBI nr
Match: XP_038880906.1 (bifunctional epoxide hydrolase 2-like [Benincasa hispida])

HSP 1 Score: 647.5 bits (1669), Expect = 5.8e-182
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD
Sbjct: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA
Sbjct: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120

Query: 121 LGVVTLGVPFMPPKRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRS 180
           LGVVTLGVPFMPPKRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRS
Sbjct: 121 LGVVTLGVPFMPPKRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRS 180

Query: 181 EIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGVK 240
           EIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGVK
Sbjct: 181 EIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGVK 240

Query: 241 DPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEEV 300
           DPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEEV
Sbjct: 241 DPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEEV 300

Query: 301 NHLLLSFLAKHT 313
           NHLLLSFLAKHT
Sbjct: 301 NHLLLSFLAKHT 312

BLAST of Bhi03G000339 vs. NCBI nr
Match: XP_004134721.1 (bifunctional epoxide hydrolase 2 [Cucumis sativus] >KGN49209.1 hypothetical protein Csa_003299 [Cucumis sativus])

HSP 1 Score: 594.0 bits (1530), Expect = 7.7e-166
Identity = 285/313 (91.05%), Postives = 300/313 (95.85%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MDK +H FVEV+GGL+IHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVA+AGFRAI +D
Sbjct: 1   MDKFEHKFVEVKGGLKIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIGLD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSDPP +PSKATYSDLI+DLLE+LD+LDI KVFLVGKDFGAMPAYYFA+KHPERA
Sbjct: 61  YRGYGLSDPPADPSKATYSDLITDLLEVLDSLDISKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGVVTLGVPFMPPKRP-NFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSR 180
           LGVVTLGVPFMPP RP NF DHLPEGFYISRWQ+PGRAEADFSRFDAKTVVRNVYILFSR
Sbjct: 121 LGVVTLGVPFMPPARPINFIDHLPEGFYISRWQKPGRAEADFSRFDAKTVVRNVYILFSR 180

Query: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGV 240
           SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGF TALKVPYRS+ EDWGV
Sbjct: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFQTALKVPYRSLGEDWGV 240

Query: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEE 300
           KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSE VK YVPKLE+IFLPEGSHFVQEQSPEE
Sbjct: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEMVKYYVPKLEVIFLPEGSHFVQEQSPEE 300

Query: 301 VNHLLLSFLAKHT 313
           +N LLL+FLAKHT
Sbjct: 301 INQLLLNFLAKHT 313

BLAST of Bhi03G000339 vs. NCBI nr
Match: XP_008439886.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0052704.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK13120.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 583.9 bits (1504), Expect = 7.9e-163
Identity = 280/313 (89.46%), Postives = 300/313 (95.85%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MDK +H FVEV+GGL+IHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVA+AGFRAI +D
Sbjct: 1   MDKFEHKFVEVKGGLKIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIGLD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSDPP +PSKATYSDLI+DLLE+LD+LDI KVFLVGKDFGAMPAYYFA+KHPERA
Sbjct: 61  YRGYGLSDPPADPSKATYSDLITDLLEVLDSLDISKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGVVTLGVPFMPP-KRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSR 180
           LGVVTLGVPFMPP +R NF D+LPEGFYISRWQ+PGRAEADFSRFDAKTVVRNVYILFSR
Sbjct: 121 LGVVTLGVPFMPPGRRINFIDYLPEGFYISRWQKPGRAEADFSRFDAKTVVRNVYILFSR 180

Query: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGV 240
           SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGF TAL+VPYRS++EDWGV
Sbjct: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFQTALQVPYRSLSEDWGV 240

Query: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEE 300
           +DPKVEIPAL VMGEKDYVLKFPG+EEYVRSE VK YVPKLE+IFLPEGSHFVQEQSPEE
Sbjct: 241 EDPKVEIPALFVMGEKDYVLKFPGMEEYVRSEMVKYYVPKLEVIFLPEGSHFVQEQSPEE 300

Query: 301 VNHLLLSFLAKHT 313
           VN LLL+FLAKHT
Sbjct: 301 VNQLLLNFLAKHT 313

BLAST of Bhi03G000339 vs. NCBI nr
Match: XP_022142376.1 (bifunctional epoxide hydrolase 2-like [Momordica charantia])

HSP 1 Score: 581.6 bits (1498), Expect = 3.9e-162
Identity = 273/312 (87.50%), Postives = 297/312 (95.19%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MD+IQHVFV+VQG L+IHVA+IGTGSNVV+FLHGFPEIWYSWRHQMIA+A+AGFRAIA D
Sbjct: 1   MDQIQHVFVQVQGALQIHVAQIGTGSNVVLFLHGFPEIWYSWRHQMIALANAGFRAIAPD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSD P EPSKATY+DLI DLL+ILDTL IPKVFLVGKDFGAMPAYYFA+KHPERA
Sbjct: 61  YRGYGLSDAPAEPSKATYADLIGDLLQILDTLHIPKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGVVTLGVPFMPPKRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRS 180
           LGVVTLGVPFMPP+   FQ HLPEGFYISRWQEPGRAE+DF RFDAKTVVRN+YILFSRS
Sbjct: 121 LGVVTLGVPFMPPEPIKFQSHLPEGFYISRWQEPGRAESDFGRFDAKTVVRNIYILFSRS 180

Query: 181 EIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGVK 240
           E+PIA+ENQEIMDLVDSSTPLPPWFTEEDLAAYG+LY+KSGF TALKVPYRS+ EDWG+K
Sbjct: 181 EVPIAEENQEIMDLVDSSTPLPPWFTEEDLAAYGDLYKKSGFDTALKVPYRSLGEDWGIK 240

Query: 241 DPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEEV 300
           DPKVEIPALL+MGEKDYVLKFPGIEEYVRSEKVKDYVPKLEII+L EGSHFVQEQSPEE+
Sbjct: 241 DPKVEIPALLIMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIYLAEGSHFVQEQSPEEI 300

Query: 301 NHLLLSFLAKHT 313
           N LLL+FL+KHT
Sbjct: 301 NQLLLNFLSKHT 312

BLAST of Bhi03G000339 vs. NCBI nr
Match: XP_022950358.1 (bifunctional epoxide hydrolase 2-like [Cucurbita moschata])

HSP 1 Score: 569.3 bits (1466), Expect = 2.0e-158
Identity = 272/313 (86.90%), Postives = 296/313 (94.57%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MDKI+H+FVEV GGL+IHVA +GTGSNVVVFLHGFPEIWYSWRHQMIAVA+AGFRAIA+D
Sbjct: 1   MDKIEHIFVEVHGGLKIHVAAVGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIALD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSD P EPSK TY+DLI+DLL+ILDTLDIPKVFLVGKDFGAMPAYYFAIK+P+RA
Sbjct: 61  YRGYGLSDSPAEPSKTTYNDLITDLLQILDTLDIPKVFLVGKDFGAMPAYYFAIKNPDRA 120

Query: 121 LGVVTLGVPFMPPKRPNFQD-HLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSR 180
           LGVVTLGVPF+P K P F++ HLPEGFYISRWQEPGRAEADF RFD KTVV+NVY+LFSR
Sbjct: 121 LGVVTLGVPFLPTK-PKFEEGHLPEGFYISRWQEPGRAEADFGRFDPKTVVKNVYVLFSR 180

Query: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGV 240
           SEIPIAQE+QE+MDLVDSSTPLPPWF+EEDLA YGELYEKSGF TALKVPYRS++ED GV
Sbjct: 181 SEIPIAQEHQEVMDLVDSSTPLPPWFSEEDLAVYGELYEKSGFETALKVPYRSIDEDLGV 240

Query: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEE 300
           KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEI+FLPEGSHFVQEQ PE+
Sbjct: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIVFLPEGSHFVQEQCPEQ 300

Query: 301 VNHLLLSFLAKHT 313
           VN LLL+FL KHT
Sbjct: 301 VNQLLLTFLVKHT 312

BLAST of Bhi03G000339 vs. ExPASy TrEMBL
Match: A0A0A0KK45 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517170 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 3.7e-166
Identity = 285/313 (91.05%), Postives = 300/313 (95.85%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MDK +H FVEV+GGL+IHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVA+AGFRAI +D
Sbjct: 1   MDKFEHKFVEVKGGLKIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIGLD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSDPP +PSKATYSDLI+DLLE+LD+LDI KVFLVGKDFGAMPAYYFA+KHPERA
Sbjct: 61  YRGYGLSDPPADPSKATYSDLITDLLEVLDSLDISKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGVVTLGVPFMPPKRP-NFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSR 180
           LGVVTLGVPFMPP RP NF DHLPEGFYISRWQ+PGRAEADFSRFDAKTVVRNVYILFSR
Sbjct: 121 LGVVTLGVPFMPPARPINFIDHLPEGFYISRWQKPGRAEADFSRFDAKTVVRNVYILFSR 180

Query: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGV 240
           SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGF TALKVPYRS+ EDWGV
Sbjct: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFQTALKVPYRSLGEDWGV 240

Query: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEE 300
           KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSE VK YVPKLE+IFLPEGSHFVQEQSPEE
Sbjct: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEMVKYYVPKLEVIFLPEGSHFVQEQSPEE 300

Query: 301 VNHLLLSFLAKHT 313
           +N LLL+FLAKHT
Sbjct: 301 INQLLLNFLAKHT 313

BLAST of Bhi03G000339 vs. ExPASy TrEMBL
Match: A0A5D3CNW3 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007300 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 3.8e-163
Identity = 280/313 (89.46%), Postives = 300/313 (95.85%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MDK +H FVEV+GGL+IHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVA+AGFRAI +D
Sbjct: 1   MDKFEHKFVEVKGGLKIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIGLD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSDPP +PSKATYSDLI+DLLE+LD+LDI KVFLVGKDFGAMPAYYFA+KHPERA
Sbjct: 61  YRGYGLSDPPADPSKATYSDLITDLLEVLDSLDISKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGVVTLGVPFMPP-KRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSR 180
           LGVVTLGVPFMPP +R NF D+LPEGFYISRWQ+PGRAEADFSRFDAKTVVRNVYILFSR
Sbjct: 121 LGVVTLGVPFMPPGRRINFIDYLPEGFYISRWQKPGRAEADFSRFDAKTVVRNVYILFSR 180

Query: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGV 240
           SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGF TAL+VPYRS++EDWGV
Sbjct: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFQTALQVPYRSLSEDWGV 240

Query: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEE 300
           +DPKVEIPAL VMGEKDYVLKFPG+EEYVRSE VK YVPKLE+IFLPEGSHFVQEQSPEE
Sbjct: 241 EDPKVEIPALFVMGEKDYVLKFPGMEEYVRSEMVKYYVPKLEVIFLPEGSHFVQEQSPEE 300

Query: 301 VNHLLLSFLAKHT 313
           VN LLL+FLAKHT
Sbjct: 301 VNQLLLNFLAKHT 313

BLAST of Bhi03G000339 vs. ExPASy TrEMBL
Match: A0A1S3B0J7 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103484540 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 3.8e-163
Identity = 280/313 (89.46%), Postives = 300/313 (95.85%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MDK +H FVEV+GGL+IHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVA+AGFRAI +D
Sbjct: 1   MDKFEHKFVEVKGGLKIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIGLD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSDPP +PSKATYSDLI+DLLE+LD+LDI KVFLVGKDFGAMPAYYFA+KHPERA
Sbjct: 61  YRGYGLSDPPADPSKATYSDLITDLLEVLDSLDISKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGVVTLGVPFMPP-KRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSR 180
           LGVVTLGVPFMPP +R NF D+LPEGFYISRWQ+PGRAEADFSRFDAKTVVRNVYILFSR
Sbjct: 121 LGVVTLGVPFMPPGRRINFIDYLPEGFYISRWQKPGRAEADFSRFDAKTVVRNVYILFSR 180

Query: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGV 240
           SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGF TAL+VPYRS++EDWGV
Sbjct: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFQTALQVPYRSLSEDWGV 240

Query: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEE 300
           +DPKVEIPAL VMGEKDYVLKFPG+EEYVRSE VK YVPKLE+IFLPEGSHFVQEQSPEE
Sbjct: 241 EDPKVEIPALFVMGEKDYVLKFPGMEEYVRSEMVKYYVPKLEVIFLPEGSHFVQEQSPEE 300

Query: 301 VNHLLLSFLAKHT 313
           VN LLL+FLAKHT
Sbjct: 301 VNQLLLNFLAKHT 313

BLAST of Bhi03G000339 vs. ExPASy TrEMBL
Match: A0A6J1CM01 (bifunctional epoxide hydrolase 2-like OS=Momordica charantia OX=3673 GN=LOC111012513 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.9e-162
Identity = 273/312 (87.50%), Postives = 297/312 (95.19%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MD+IQHVFV+VQG L+IHVA+IGTGSNVV+FLHGFPEIWYSWRHQMIA+A+AGFRAIA D
Sbjct: 1   MDQIQHVFVQVQGALQIHVAQIGTGSNVVLFLHGFPEIWYSWRHQMIALANAGFRAIAPD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSD P EPSKATY+DLI DLL+ILDTL IPKVFLVGKDFGAMPAYYFA+KHPERA
Sbjct: 61  YRGYGLSDAPAEPSKATYADLIGDLLQILDTLHIPKVFLVGKDFGAMPAYYFALKHPERA 120

Query: 121 LGVVTLGVPFMPPKRPNFQDHLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSRS 180
           LGVVTLGVPFMPP+   FQ HLPEGFYISRWQEPGRAE+DF RFDAKTVVRN+YILFSRS
Sbjct: 121 LGVVTLGVPFMPPEPIKFQSHLPEGFYISRWQEPGRAESDFGRFDAKTVVRNIYILFSRS 180

Query: 181 EIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGVK 240
           E+PIA+ENQEIMDLVDSSTPLPPWFTEEDLAAYG+LY+KSGF TALKVPYRS+ EDWG+K
Sbjct: 181 EVPIAEENQEIMDLVDSSTPLPPWFTEEDLAAYGDLYKKSGFDTALKVPYRSLGEDWGIK 240

Query: 241 DPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEEV 300
           DPKVEIPALL+MGEKDYVLKFPGIEEYVRSEKVKDYVPKLEII+L EGSHFVQEQSPEE+
Sbjct: 241 DPKVEIPALLIMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIYLAEGSHFVQEQSPEEI 300

Query: 301 NHLLLSFLAKHT 313
           N LLL+FL+KHT
Sbjct: 301 NQLLLNFLSKHT 312

BLAST of Bhi03G000339 vs. ExPASy TrEMBL
Match: A0A6J1GEN9 (bifunctional epoxide hydrolase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453470 PE=4 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.8e-159
Identity = 272/313 (86.90%), Postives = 296/313 (94.57%), Query Frame = 0

Query: 1   MDKIQHVFVEVQGGLEIHVAEIGTGSNVVVFLHGFPEIWYSWRHQMIAVADAGFRAIAVD 60
           MDKI+H+FVEV GGL+IHVA +GTGSNVVVFLHGFPEIWYSWRHQMIAVA+AGFRAIA+D
Sbjct: 1   MDKIEHIFVEVHGGLKIHVAAVGTGSNVVVFLHGFPEIWYSWRHQMIAVANAGFRAIALD 60

Query: 61  YRGYGLSDPPHEPSKATYSDLISDLLEILDTLDIPKVFLVGKDFGAMPAYYFAIKHPERA 120
           YRGYGLSD P EPSK TY+DLI+DLL+ILDTLDIPKVFLVGKDFGAMPAYYFAIK+P+RA
Sbjct: 61  YRGYGLSDSPAEPSKTTYNDLITDLLQILDTLDIPKVFLVGKDFGAMPAYYFAIKNPDRA 120

Query: 121 LGVVTLGVPFMPPKRPNFQD-HLPEGFYISRWQEPGRAEADFSRFDAKTVVRNVYILFSR 180
           LGVVTLGVPF+P K P F++ HLPEGFYISRWQEPGRAEADF RFD KTVV+NVY+LFSR
Sbjct: 121 LGVVTLGVPFLPTK-PKFEEGHLPEGFYISRWQEPGRAEADFGRFDPKTVVKNVYVLFSR 180

Query: 181 SEIPIAQENQEIMDLVDSSTPLPPWFTEEDLAAYGELYEKSGFHTALKVPYRSMNEDWGV 240
           SEIPIAQE+QE+MDLVDSSTPLPPWF+EEDLA YGELYEKSGF TALKVPYRS++ED GV
Sbjct: 181 SEIPIAQEHQEVMDLVDSSTPLPPWFSEEDLAVYGELYEKSGFETALKVPYRSIDEDLGV 240

Query: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIIFLPEGSHFVQEQSPEE 300
           KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEI+FLPEGSHFVQEQ PE+
Sbjct: 241 KDPKVEIPALLVMGEKDYVLKFPGIEEYVRSEKVKDYVPKLEIVFLPEGSHFVQEQCPEQ 300

Query: 301 VNHLLLSFLAKHT 313
           VN LLL+FL KHT
Sbjct: 301 VNQLLLTFLVKHT 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G51000.13.1e-5637.22alpha/beta-Hydrolases superfamily protein [more]
AT4G02340.19.0e-5634.89alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.13.0e-5135.67soluble epoxide hydrolase [more]
AT3G05600.11.9e-5034.46alpha/beta-Hydrolases superfamily protein [more]
AT2G26750.18.2e-4934.62alpha/beta-Hydrolases superfamily protein [more]
Match NameE-valueIdentityDescription
P349137.5e-3933.86Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P349141.4e-3731.88Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
P802991.8e-3731.56Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Q6Q2C25.4e-3732.81Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
I6YGS01.9e-3431.41Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Match NameE-valueIdentityDescription
XP_038880906.15.8e-182100.00bifunctional epoxide hydrolase 2-like [Benincasa hispida][more]
XP_004134721.17.7e-16691.05bifunctional epoxide hydrolase 2 [Cucumis sativus] >KGN49209.1 hypothetical prot... [more]
XP_008439886.17.9e-16389.46PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0052704.1 bi... [more]
XP_022142376.13.9e-16287.50bifunctional epoxide hydrolase 2-like [Momordica charantia][more]
XP_022950358.12.0e-15886.90bifunctional epoxide hydrolase 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KK453.7e-16691.05AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517... [more]
A0A5D3CNW33.8e-16389.46Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3B0J73.8e-16389.46bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103484540 PE... [more]
A0A6J1CM011.9e-16287.50bifunctional epoxide hydrolase 2-like OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1GEN99.8e-15986.90bifunctional epoxide hydrolase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 99..112
score: 33.0
coord: 113..126
score: 33.25
coord: 53..68
score: 42.24
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 27..133
e-value: 6.8E-21
score: 75.1
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 113..126
score: 26.95
coord: 246..262
score: 28.88
coord: 99..112
score: 32.14
coord: 53..68
score: 51.7
coord: 33..51
score: 52.15
coord: 285..307
score: 27.67
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..312
e-value: 3.6E-87
score: 295.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..311
NoneNo IPR availablePANTHERPTHR43329:SF106BIFUNCTIONAL EPOXIDE HYDROLASE 2-LIKE ISOFORM X1coord: 2..311
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 2..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000339Bhi03M000339mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity