Bhi03G000203 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000203
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPhenylalanyl-tRNA synthetase
Locationchr3: 5237672 .. 5243777 (+)
RNA-Seq ExpressionBhi03G000203
SyntenyBhi03G000203
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCGACTCTGTCACTGACTCAAGCTCTTCTCTTCACCAATGCGTCTAATCTTCTACGCAGAAACTGCCTCAGAACCTTTGTCTTCTCTGCATCTTTCTCCTCCTCCTCAGCTCTTTCCTCCGACAACGTTCACCGGAAGAAATGGAGACAGCCGGTCTCTTCACTTCTTGAGCTCGGCGGAATCGCAATTGCCAAAGATGGTAAATTATTCTTTTCTGTTTTATTTTACCCGCCTATGCCGTTTTCTTGTTTTGCTAATCCGCGTCTGTATTCGGTGTCATGGATTCATTCCTATTCGTGAGATTTTCTGGTGGAACTTCCACGATTTTGTTGAATTTTGTTTAGCGATGTGAATATAGTTACTATCTTGGATAATTGTAATTTTTCATGTGATAATTTTGCTGCTTCGTCTTTTGGACTTTAGAAACAACACCGAACTTTCTCTGATTCTTCGCTTAGTCTACGATATGTTTTAACATTGCGACTGAGCACCAGGGAATGGTAAGGGGCTCTGTTTCACGGACCTCTGAGAACTTTTTCTGAAAAAAATCCGTCAGTGTACTTTTTCCATATTGTATTCTTCTATTTTCTAATTTAGAAAGCTGTTTGAAGAGTGAGTTTTCCCCTCCTCGTCTTTATTAATTCTCTTGTTGATGTCAACTACAACATATGTAGATCACTCTATACTAAGGAGAACGTTAAACAGCTGGGTACAGCATCTGTGCTTATTTCTAATTTCTAATGAAGAAAATAACTTTTAAGTTCAATTTCTAAACATCCTCAAAGTGTTTGGTTCTGCCGTTCTGAGCCATAATTAATGATCTTTCTAGATTCCTGTTTTATATTGGCGTTTATGTAAGATAACTACTTCCAAAAATGGTTATGCGGTTGCTATTTGTTGCTTCATTTTCCCCCTGATTTGAAGTTAAATAATTATTCATTGCAGTTCATATTTCTCTTTTGTTAATTCAACGAAACTATTTTATGGATTTAATTTTCTAGGTGCTTGTTGAACTGTTCTGCCAATTAACCATATTTTTATAAAATTTATATCGCCTGAAGTTAAATTTTAATGTGCACTAGTTACTAATATTATTGGTGGCTTGCAGATGTTGTGAGGGATGATCCTACAAACAATGTCCCAGATAATATTTTCTCAAAACTTGGTATGCAACTCCACCGAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAAATATTTTGATATCAATTACTCAAATAAGTTCAATAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGGTAAGCCTTTTAAACTCATCAATTGGTTTTGAGGCTGAAAGGAAGCCCATTCGATGTCTATCTCTATCCCGAGGAAATTTTAGTTTCCAGAAAATTGAGTAGTCCAGTTGCCAATTCTCTGGAAAAATGAGTTTTACGCTGCTTTAGAACCTTTGTATGTGAATCTTTGATCTAGAAAATTGCAAGTATTTCAACCTTTCTGCATGAAAAGGACTAATGACAGAAAACATATAACTGCTCGATTTCAGCAACTTGTATTGCTTCTGTTGTTCAGTATATTGTGCGACCAGACTTAGTTTGAAGATTTATCCACGCCTCAAATCTTTTGCCATGTTTTTTATTTTTTTTTGGGGGGTGGGGGGGGGGGGCGGGGGCGGGCACCAAAGGAAATCTGCTCCTACGCAGAATTAAATGCAGGTGCTGGTTTAGCTGCAACTGCAAGTACTTGCTGACTTATAGGGGATGGAATTTTATAACCACCCTTCTTACGCTTGGAAGTGTTGGTTTTCTTAGTAAATGGTGATTTAGTTAAATCTGAGTTTTGCTCACATTCTTCTTTTATAACTCTGCTAGGACACTGACAACATTCCTACACCCTTTGACCCTTTGCATTCTTTAAGAGTTCCAGAAGAATCTGTATATCTTAGTTTTCTTTCTCTCCTCCTTTTACACCTATAGTTATGCAGTATGAAAGCATGTAAAACTTTCATTTAGGTGTTAATTGCATCAGCTATCTTTTGTAGTTGAAACTTTTTCTTAATATAGTGCACAGAACAAATCATCATAACTTGGCACTTCGGTGGAAAGGACAAGCCAAAACTCATAGTGTAAAGAGGGTTACAGGTTCAGGTCTTGATTACTACTTATTTAGGATATTAATAATATCCCTTGAATATCCTGACTCCACTATCATCGAACCAAGTGGTTGTCTTATCATGTATCAGCTATAAATGTGCATTGCACGTGTAACCAAAAATGCTTTTGGTTCCTAGAGTTTGAACTTGGTTTTTATTTGGTCATCATTCCATCAAGTCAACTCAGGGACAAATTAAAAATCAAATTCAAACCTTGGGGTCCAAACATGTTGCCTATGAAACCTTGGAATTAAATTGATACTAAATTCAAACTTAAGACCAAAAGCTAATACAAGCCTTAGGGACCGAACATCTTATGGACCAAATGGAAACTTAATTCAAATTGACCAAAGTGTTCGGACATATTTGGATTGAAAAAGAAAAGTACTAAATATTTTTTGGAAGAAGGCAGGATTAGTTTCGTAGTTGTCAACTTTCCTGGCAACTGGTTAAGTTTTTTTTCTCCTTCATTCCAATAGCTTATGTGTTATTTATTTATATATAATAGTTATTATTTTTAAACATACAATGTCTCTTATCGAATCCTATGATTATGGCTGCAGAATTTTGATGATGTATTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCATCAAGCGGAGCTGTTGAGAAGAGGATATACTCAGTTTCTTGTGACAGGAGACGTTTACCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGGTAGTCTAAATGAATGCAGTAATCAATTCATGACTTTTGCTGAATTTACCAAAATTTTCATTCAATGAATTTCGTTGCCACTTCCCACAGATGGAAGCTGTCCGTGTTTTTTCTCTAAATGATTGGGAGGCATCTGGCACAGATGGAACTTCTTATGCTGCTGATGACTTGAAGAAATGCTTGGAAGGTTTGGCAAGGCATTTATTTGGTAAGTTTGTATTAATCAAGGCACCCATGATTCTTCCGTTTCTGCCTTAGGTTTGCGGTTGGAATTCCAATGCAAATGATTTTGGGGAAAAAACAATAACACTTGCAGGATAAGCTGTTAATCATAAAAACTATAGGAGTAACAAATATAATGATCAGATAAGTATTAATGAGTATTATTTTGCCTATGATCATTAAGGGGGCTTTTGGGCTTTCGAGCGACTCTTTCAACTGCCACCTAATCTCCCAACAAGTTTCTGGGCACTGAGTTGAATTATTAAGTCTAGAGTTGTTTAGTTTGAAGAGTTAGTCCATGTTTGGGGTGTAAAGTTGTTTAATATGGATTCTGAAGTCTATATGCTGACTTTTTTTTTTTTTGCTACTTTTGATTTAATATGCAATTAAAGCTATATTTCAATCCTTACAAAATTGATTTTAAAATTCATTACATTATTTGTTCTACCCCACTCAAATTTGATATGGGATTGAGTATGAAGGCAACAAATTTTCTTAATGTGAGATCAACTCTAGATGGCTTGAATTTGGATTTTACATTTTCTAAAAAAAAAAAAAAACATTTGGGATAAAGTTTGGACTATGTCCAAATTTAATTGAACCTAATTGTTAGGTTCTATCCAAATTTTGGTTTTGATTTGGAGTAGAATTCTAAGATGGATTTTGTGGAATTTGATTTGGGATGAAAATTTGATTAAATTGAATCAACATTAATCTTTTGTTTGATTTAAATTTGACCAATTTGTGGATTATAATTTGAATCAAAATTTCTAATTTGATAAAGATTCAACATTGATATGTTTTTTTTAAAAAAAAAAAATTTAATCAACACTTTTCTTAAAAAAAATTGTGGGGGTGGAGTTATTGTAATTATATTGGATGGATAGAGTGTAGGAAAATAGAGTTCTTCAATAAATGTGCAAACACTATTTGAAGTCAGTAAGTGTAGTTATTTGCGAAGTATGTGGGCCAAACAAAGAGTTTGATTATTTTTAATCAAATCCACCCAACTCAAGTTGGTGGTCCAAATGCCCCTAAAAAGCTTCTTCCAAACGTGGTTACATTTTTAAGATAACAATGAACCCAACTTACGTAATTATGTTTTGGTTTTTAAAACCAAATTCTTTAATTTTGCAGTGGTTGTACACATGATGGAGCTATGTTTTCAATAACTAATCTCTTAAAACTTCCAGGTGCTGTGGAAATGCGCTGGGTTGATGCATATTTCCCATTTACAAACCCATCATTCGAACTCGAGATTTTCTTTCAGGTATGTCAGATGATCATGAAGTCAACTTGCTCTTCAGAATTCAATACTTTGATCATGGTGCACTGCATTGCTTCTTTTATAAATAGCTTTCATTGTCACCAATATGCATTCAAGTTTATCTATGTCAAGTTTTACACAGCATTAGGTTTATCATAAGAACCATACAACTTTTTAACTTTTGATAAGTTAGACAAGGGAACCACAAAGTGTGAATGTGTTAGGGAGGGGACGTAGATGCTCTCTCTCTCTCTCTCTCTCTTTTTAAATAAAAAAAAATATATTATTACCATTATTAATATTTTTGTGTGTGTGTGTTGGGGTGGGGGGGATTTAGTAATCAACCAGACAAAGGCAAGAGGGCAACAAAAAGTTCCTCTATTTGGGATTGGCCACAACCATTTCTCAGCTATTATTTCCATCCATGAATCATATAAAATAATGAATAGTTAGTGCTTGAATTATGAGATGAGATTTCCTTGACAACTTATTTATGTTGCATTTGCTGTTGGTTCCTTCTTGAATGAAATTTATTCTTTGTTCTCTCTTTCTTTTTCAAACAAAAATATAATAAAAATTAATCCTGTATCTGAGGGTTATTTGATGCGCTACTTATGCAGGAGAAATGGCTGGAAGTATTGGGATGTGGGGTGATGGAGCAAGAAATATTGCAAAAATGTGGCAAAGAGAATCATGTTGCATGGGCTTTTGGTCTTGGATTGGAAAGGCTAGCCATGGTTCTTTTTGATATACCTGATGTCCGATTATTTTGGTCAGCCGATGAGCGATTTACCTCGCAGGTAGCTAATTACATCATGCAAACTGTACTCTTCCGTTTTTTCCACTATTTTCTCTTGGCATTCTCAAAAATAAATTATCCAATATCACGTTTGGGCTTTTTCTTTGCAGTTCTCAAAGGGCAAGCTAGGTGTTAAATTTAAACCATTTTCTAAGGTACTATTACCAAACATTTTAGGCATCGAGGGATAGCAATATTTGATTTCCTGAAAACGCAATTTTTTTTATTTTATTTCTTTTAGTAACATCTAACTGGTTTCAGTATGACTTCTAAGAAATTATATCCTCTAAATTATTTTAAAAAGCTTTAGTAATTGTATCTGCTGCAGTTTCCACCTTGCTACAAGGATATTAGTTTTTGGATCAATGATTCTTTCACCGAGAATAATTTGTGTGAAGTCGTGAGAGGAATTGCCGGGGACCTTGTTGAAGAGGTATATTATTAACTGTTTTCAGGGCAAGAGTTCCTTAAATAATTTTCGTGAAGTAAATGCTTATGGTAGGAGCATGAGAAAATTTTTCTTTTTGAAGTACATGCTTAAGATAATCTGGTCTGTTCAGTTATCTGACAGTTGCCCCTCGTTTAGTGAAATAATCTCATTCTTCACAAATGAATGCTGGCAGGTGCAACTGATAGACAGTTTTACCAACGAGAAGAAAGGGATGACAAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGGTATTTATCAACTTTAAGTTGTATCCATATTGTATATTAATTTGTTTAAAAAAGAAGTTTGGATACAGGCTTCATATATTTTGTGGTTGCAAATGCAGAGGAACGTGAGAGAACAGGTTCAGAGCAGGTTGAATGTTGTCTTAAGATGA

mRNA sequence

ATGGCATCGACTCTGTCACTGACTCAAGCTCTTCTCTTCACCAATGCGTCTAATCTTCTACGCAGAAACTGCCTCAGAACCTTTGTCTTCTCTGCATCTTTCTCCTCCTCCTCAGCTCTTTCCTCCGACAACGTTCACCGGAAGAAATGGAGACAGCCGGTCTCTTCACTTCTTGAGCTCGGCGGAATCGCAATTGCCAAAGATGATGTTGTGAGGGATGATCCTACAAACAATGTCCCAGATAATATTTTCTCAAAACTTGGTATGCAACTCCACCGAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAAATATTTTGATATCAATTACTCAAATAAGTTCAATAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGAATTTTGATGATGTATTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCATCAAGCGGAGCTGTTGAGAAGAGGATATACTCAGTTTCTTGTGACAGGAGACGTTTACCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGATGGAAGCTGTCCGTGTTTTTTCTCTAAATGATTGGGAGGCATCTGGCACAGATGGAACTTCTTATGCTGCTGATGACTTGAAGAAATGCTTGGAAGGTTTGGCAAGGCATTTATTTGGTGCTGTGGAAATGCGCTGGGTTGATGCATATTTCCCATTTACAAACCCATCATTCGAACTCGAGATTTTCTTTCAGGAGAAATGGCTGGAAGTATTGGGATGTGGGGTGATGGAGCAAGAAATATTGCAAAAATGTGGCAAAGAGAATCATGTTGCATGGGCTTTTGGTCTTGGATTGGAAAGGCTAGCCATGGTTCTTTTTGATATACCTGATGTCCGATTATTTTGGTCAGCCGATGAGCGATTTACCTCGCAGTTCTCAAAGGGCAAGCTAGGTGTTAAATTTAAACCATTTTCTAAGTTTCCACCTTGCTACAAGGATATTAGTTTTTGGATCAATGATTCTTTCACCGAGAATAATTTGTGTGAAGTCGTGAGAGGAATTGCCGGGGACCTTGTTGAAGAGGTGCAACTGATAGACAGTTTTACCAACGAGAAGAAAGGGATGACAAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGAGGAACGTGAGAGAACAGGTTCAGAGCAGGTTGAATGTTGTCTTAAGATGA

Coding sequence (CDS)

ATGGCATCGACTCTGTCACTGACTCAAGCTCTTCTCTTCACCAATGCGTCTAATCTTCTACGCAGAAACTGCCTCAGAACCTTTGTCTTCTCTGCATCTTTCTCCTCCTCCTCAGCTCTTTCCTCCGACAACGTTCACCGGAAGAAATGGAGACAGCCGGTCTCTTCACTTCTTGAGCTCGGCGGAATCGCAATTGCCAAAGATGATGTTGTGAGGGATGATCCTACAAACAATGTCCCAGATAATATTTTCTCAAAACTTGGTATGCAACTCCACCGAAGAGATAACCATCCACTTGGCATTCTGAAGAATGAGATATACAAATATTTTGATATCAATTACTCAAATAAGTTCAATAAGTTTGATGACCTCAGTCCAATCGTTTCTGTGAAGGAGAATTTTGATGATGTATTGGTTCCAGAAGATCATGTAAGCCGAAGTTATAATGATACATACTACATTGACTCGCAAACTGTTCTGAGATGTCATACAAGTGCTCATCAAGCGGAGCTGTTGAGAAGAGGATATACTCAGTTTCTTGTGACAGGAGACGTTTACCGTCGGGATTCCATTGATTCAACTCATTACCCTGTATTTCATCAGATGGAAGCTGTCCGTGTTTTTTCTCTAAATGATTGGGAGGCATCTGGCACAGATGGAACTTCTTATGCTGCTGATGACTTGAAGAAATGCTTGGAAGGTTTGGCAAGGCATTTATTTGGTGCTGTGGAAATGCGCTGGGTTGATGCATATTTCCCATTTACAAACCCATCATTCGAACTCGAGATTTTCTTTCAGGAGAAATGGCTGGAAGTATTGGGATGTGGGGTGATGGAGCAAGAAATATTGCAAAAATGTGGCAAAGAGAATCATGTTGCATGGGCTTTTGGTCTTGGATTGGAAAGGCTAGCCATGGTTCTTTTTGATATACCTGATGTCCGATTATTTTGGTCAGCCGATGAGCGATTTACCTCGCAGTTCTCAAAGGGCAAGCTAGGTGTTAAATTTAAACCATTTTCTAAGTTTCCACCTTGCTACAAGGATATTAGTTTTTGGATCAATGATTCTTTCACCGAGAATAATTTGTGTGAAGTCGTGAGAGGAATTGCCGGGGACCTTGTTGAAGAGGTGCAACTGATAGACAGTTTTACCAACGAGAAGAAAGGGATGACAAGTCACTGTTACAGGATTGCTTACCGATCTATGGAACGGTCACTCACAGATGAAGAAATCAATGATTTGCAGAGGAACGTGAGAGAACAGGTTCAGAGCAGGTTGAATGTTGTCTTAAGATGA

Protein sequence

MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLELGGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNKFDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLFGAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGLERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTENNLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVREQVQSRLNVVLR
Homology
BLAST of Bhi03G000203 vs. TAIR 10
Match: AT3G58140.1 (phenylalanyl-tRNA synthetase class IIc family protein )

HSP 1 Score: 668.3 bits (1723), Expect = 4.1e-192
Identity = 324/430 (75.35%), Postives = 371/430 (86.28%), Query Frame = 0

Query: 4   TLSLTQALLFTNAS-NLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLELGG 63
           T+   Q+ +F+ AS  LL  N  + F F +SFSSS+A S   +  +K R P+ S +++GG
Sbjct: 2   TVFSVQSTIFSRASVALLSSNGFKRFSFVSSFSSSAAYSPPKM--RKRRYPIVSAVDIGG 61

Query: 64  IAIAKDDVVR-DDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNKF 123
           +AIA++DVVR DDPTNNVPD+IFSKLGMQLHRRD HP+GILKN IY YFD NYSNKF KF
Sbjct: 62  VAIARNDVVREDDPTNNVPDSIFSKLGMQLHRRDKHPIGILKNAIYDYFDSNYSNKFEKF 121

Query: 124 DDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLV 183
           +DLSPIV+ K+NFDDVLVP DHVSRS NDTYY+DSQTVLRCHTSAHQAELLR+G+++FLV
Sbjct: 122 EDLSPIVTTKQNFDDVLVPADHVSRSLNDTYYVDSQTVLRCHTSAHQAELLRKGHSRFLV 181

Query: 184 TGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLFG 243
           TGDVYRRDSIDSTHYPVFHQME   VFS  DW  SG D T YAA+DLKKCLEGLARHLFG
Sbjct: 182 TGDVYRRDSIDSTHYPVFHQMEGFCVFSPEDWNGSGKDSTLYAAEDLKKCLEGLARHLFG 241

Query: 244 AVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGLE 303
           +VEMRWVD YFPFTNPSFELEI+F+E WLEVLGCGV EQ IL++ G EN+VAWAFGLGLE
Sbjct: 242 SVEMRWVDTYFPFTNPSFELEIYFKEDWLEVLGCGVTEQVILKQSGLENNVAWAFGLGLE 301

Query: 304 RLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTENN 363
           RLAMVLFDIPD+R FWS+DERFTSQF KG+LGVKFKP+SK+PPCYKDISFWI+D FTENN
Sbjct: 302 RLAMVLFDIPDIRFFWSSDERFTSQFGKGELGVKFKPYSKYPPCYKDISFWISDLFTENN 361

Query: 364 LCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVREQ 423
            CEVVRGIAGDLVEEV+LID FTN+KKG+TSHCYRI +RSMERSLTDEE+NDLQ  VR++
Sbjct: 362 FCEVVRGIAGDLVEEVKLIDQFTNKKKGLTSHCYRIVFRSMERSLTDEEVNDLQSKVRDE 421

Query: 424 VQSRLNVVLR 432
           VQ +LNV LR
Sbjct: 422 VQKKLNVELR 429

BLAST of Bhi03G000203 vs. TAIR 10
Match: AT4G39280.1 (phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative )

HSP 1 Score: 66.2 bits (160), Expect = 7.2e-11
Identity = 55/200 (27.50%), Postives = 92/200 (46.00%), Query Frame = 0

Query: 139 VPEDHV-------------SRSYNDTYYID--SQTVLRCHTSAHQAELL----RRGYT-- 198
           +PED+V             SR YN  +  +  ++ +LR HT+A  + +L    ++ +   
Sbjct: 277 LPEDYVERVKRVHESGGYGSRGYNYDWKREEANKNLLRTHTTAVSSRMLYALAQKPFVPK 336

Query: 199 QFLVTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLAR 258
           ++     V+R +++D TH   FHQ+E +                     DL   LE    
Sbjct: 337 KYFSIDRVFRNEAVDRTHLAEFHQIEGL------------ICDRGLTLGDLIGVLEDFFS 396

Query: 259 HLFGAVEMRWVDAYFPFTNPSFELEIFFQ--EKWLEVLGCGVMEQEILQKCGKENHV-AW 315
            L G  ++R+  AY P+T PS E+  + +   KW+E+   G+   E+L   G    V   
Sbjct: 397 RL-GMSKLRFKPAYNPYTEPSMEIFSYHEGLGKWVEIGNSGMFRPEMLLPMGLPEDVRVI 456

BLAST of Bhi03G000203 vs. TAIR 10
Match: AT4G39280.2 (phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative )

HSP 1 Score: 66.2 bits (160), Expect = 7.2e-11
Identity = 55/200 (27.50%), Postives = 92/200 (46.00%), Query Frame = 0

Query: 139 VPEDHV-------------SRSYNDTYYID--SQTVLRCHTSAHQAELL----RRGYT-- 198
           +PED+V             SR YN  +  +  ++ +LR HT+A  + +L    ++ +   
Sbjct: 277 LPEDYVERVKRVHESGGYGSRGYNYDWKREEANKNLLRTHTTAVSSRMLYALAQKPFVPK 336

Query: 199 QFLVTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLAR 258
           ++     V+R +++D TH   FHQ+E +                     DL   LE    
Sbjct: 337 KYFSIDRVFRNEAVDRTHLAEFHQIEGL------------ICDRGLTLGDLIGVLEDFFS 396

Query: 259 HLFGAVEMRWVDAYFPFTNPSFELEIFFQ--EKWLEVLGCGVMEQEILQKCGKENHV-AW 315
            L G  ++R+  AY P+T PS E+  + +   KW+E+   G+   E+L   G    V   
Sbjct: 397 RL-GMSKLRFKPAYNPYTEPSMEIFSYHEGLGKWVEIGNSGMFRPEMLLPMGLPEDVRVI 456

BLAST of Bhi03G000203 vs. ExPASy Swiss-Prot
Match: Q94K73 (Phenylalanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g58140 PE=1 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 5.8e-191
Identity = 324/430 (75.35%), Postives = 371/430 (86.28%), Query Frame = 0

Query: 4   TLSLTQALLFTNAS-NLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLELGG 63
           T+   Q+ +F+ AS  LL  N  + F F +SFSSS+A S   +  +K R P+ S +++GG
Sbjct: 2   TVFSVQSTIFSRASVALLSSNGFKRFSFVSSFSSSAAYSPPKM--RKRRYPIVSAVDIGG 61

Query: 64  IAIAKDDVVR-DDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNKF 123
           +AIA++DVVR DDPTNNVPD+IFSKLGMQLHRRD HP+GILKN IY YFD NYSNKF KF
Sbjct: 62  VAIARNDVVREDDPTNNVPDSIFSKLGMQLHRRDKHPIGILKNAIYDYFDSNYSNKFEKF 121

Query: 124 DDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLV 183
           +DLSPIV+ K+NFDDVLVP DHVSRS NDTYY+DSQTVLRCHTSAHQAELLR+G+++FLV
Sbjct: 122 EDLSPIVTTKQNFDDVLVPADHVSRSLNDTYYVDSQTVLRCHTSAHQAELLRKGHSRFLV 181

Query: 184 TGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLFG 243
           TGDVYRRDSIDSTHYPVFHQME   VFS  DW  SG D T YAA+DLKKCLEGLARHLFG
Sbjct: 182 TGDVYRRDSIDSTHYPVFHQMEGFCVFSPEDWNGSGKDSTLYAAEDLKKCLEGLARHLFG 241

Query: 244 AVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGLE 303
           +VEMRWVD YFPFTNPSFELEI+F+E WLEVLGCGV EQ IL++ G EN+VAWAFGLGLE
Sbjct: 242 SVEMRWVDTYFPFTNPSFELEIYFKEDWLEVLGCGVTEQVILKQSGLENNVAWAFGLGLE 301

Query: 304 RLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTENN 363
           RLAMVLFDIPD+R FWS+DERFTSQF KG+LGVKFKP+SK+PPCYKDISFWI+D FTENN
Sbjct: 302 RLAMVLFDIPDIRFFWSSDERFTSQFGKGELGVKFKPYSKYPPCYKDISFWISDLFTENN 361

Query: 364 LCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVREQ 423
            CEVVRGIAGDLVEEV+LID FTN+KKG+TSHCYRI +RSMERSLTDEE+NDLQ  VR++
Sbjct: 362 FCEVVRGIAGDLVEEVKLIDQFTNKKKGLTSHCYRIVFRSMERSLTDEEVNDLQSKVRDE 421

Query: 424 VQSRLNVVLR 432
           VQ +LNV LR
Sbjct: 422 VQKKLNVELR 429

BLAST of Bhi03G000203 vs. ExPASy Swiss-Prot
Match: Q86A68 (Phenylalanine--tRNA ligase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=mpheS PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 6.4e-105
Identity = 204/416 (49.04%), Postives = 266/416 (63.94%), Query Frame = 0

Query: 67  KDDVVRDDPTN-NVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYS---NKFNKFD 126
           K D  +  P N N+  +I  K+G  LH + +HPL I+K +I  +F    S   +KF  FD
Sbjct: 39  KIDYTKLIPENGNISKSITEKIGKNLHCKRDHPLNIIKKKIQYHFQNKLSDEEHKFQFFD 98

Query: 127 DLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFLVT 186
              P VSVKENFD++L P DHV RS NDTYY     +LR HTSAHQ++LLR     FLVT
Sbjct: 99  SFEPKVSVKENFDELLFPVDHVGRSPNDTYYFSKDQLLRTHTSAHQSQLLREQEKAFLVT 158

Query: 187 GDVYRRDSIDSTHYPVFHQMEAVRVF---------------SLNDWEASGTDGTSYAAD- 246
           GDVYRRD+ID+ HYPVFHQME V+VF               +++ +E +         D 
Sbjct: 159 GDVYRRDTIDAVHYPVFHQMEGVKVFKDKVNLAIDGKPFDETIDYYEDNNYKQLQSVKDV 218

Query: 247 --DLKKCLEGLARHLFGA-VEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEIL 306
             DLK+ LE + R + G  +++RW+DAYFPFT+PSFE+EI+FQ +WLEVLGCGV+   I+
Sbjct: 219 EKDLKQSLESMIRSVIGQDLQVRWIDAYFPFTSPSFEMEIYFQGQWLEVLGCGVVHPSIM 278

Query: 307 QKCGKENHVAWAFGLGLERLAMVLFDIPDVRLFWSADERFTSQF---------------- 366
             CG  N  AWAFG+GLERLAM+LF+IPD+RLFW+ D RF +QF                
Sbjct: 279 NNCGLSNDRAWAFGIGLERLAMILFNIPDIRLFWTEDNRFHNQFKGVDKSISTSSSSSSS 338

Query: 367 ----SKGKL------GVKFKPFSKFPPCYKDISFWIND--SFTENNLCEVVRGIAGDLVE 426
               S   L      GV+F+ FSK+P C+KD+SFW+ D  +F EN   E VR   GDLVE
Sbjct: 339 SSSSSSSTLSDIDIKGVQFQQFSKYPSCFKDVSFWLEDEENFHENKFYEFVRESCGDLVE 398

Query: 427 EVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVREQVQSRLNVVLR 432
            V L+D+FTN+K   TSHCYRI YRSM+R+LT+EEI+ LQ N+RE++++ L+V LR
Sbjct: 399 RVDLVDNFTNKKLNKTSHCYRIYYRSMDRNLTNEEIDILQFNLREKLENHLSVKLR 454

BLAST of Bhi03G000203 vs. ExPASy Swiss-Prot
Match: Q99M01 (Phenylalanine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Fars2 PE=1 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.6e-100
Identity = 189/405 (46.67%), Postives = 254/405 (62.72%), Query Frame = 0

Query: 56  SLLELGGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYS 115
           S+LE+ G +  +DD        N+   + SK+G  LH +  HPL ++K  + ++F   Y 
Sbjct: 49  SVLEILGKSYPQDD------HTNLTQKVLSKVGRNLHNQKFHPLWLIKERVKEHFYQQYM 108

Query: 116 NK-----FNKFDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAE 175
            +     F+ +D L P+V+  +NFD +L+P DH SR   D YY++   +LR HTSAHQ +
Sbjct: 109 VRSRTPLFSVYDQLPPVVTTWQNFDSLLIPADHPSRKKGDNYYLNRAHMLRAHTSAHQWD 168

Query: 176 LLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTS-------- 235
           LL  G   FLV GDVYRRD ID  HYPVFHQ+E VR+FS ++  A   DG S        
Sbjct: 169 LLHAGLNAFLVVGDVYRRDQIDCQHYPVFHQLEGVRLFSKHELFAGVKDGESLQLFEEGS 228

Query: 236 ---------------YAADDLKKCLEGLARHLFG-AVEMRWVDAYFPFTNPSFELEIFFQ 295
                              DLK+ L  L  HLFG  +E+RWVD YFPFT+PSFE+EI F+
Sbjct: 229 RSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEINFR 288

Query: 296 EKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGLERLAMVLFDIPDVRLFWSADERFTSQ 355
            +WLEVLGCGVMEQ+++   G ++ + WAFGLGLERLAMVL+DIPD+RLFWS DERF  Q
Sbjct: 289 GEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMVLYDIPDIRLFWSEDERFLKQ 348

Query: 356 F--SKGKLGVKFKPFSKFPPCYKDISFWI-NDSFTENNLCEVVRGIAGDLVEEVQLIDSF 415
           F  S     VKF+P SK+P  + DISFW+ ++++TEN+  ++VR + GDLVE+V LID F
Sbjct: 349 FLLSDINQSVKFQPLSKYPAVFNDISFWLPSENYTENDFYDIVRTVGGDLVEKVDLIDKF 408

Query: 416 TNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVREQVQSRLNV 429
            + K   TSHCYRI YR MER+L+  E+ ++ + V+E     L V
Sbjct: 409 EHPKTHRTSHCYRITYRHMERTLSQREVGNVHQAVQEAAVQLLGV 447

BLAST of Bhi03G000203 vs. ExPASy Swiss-Prot
Match: O95363 (Phenylalanine--tRNA ligase, mitochondrial OS=Homo sapiens OX=9606 GN=FARS2 PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 4.0e-99
Identity = 185/405 (45.68%), Postives = 255/405 (62.96%), Query Frame = 0

Query: 56  SLLELGGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYS 115
           S++EL G +  +DD       +N+   + +++G  LH + +HPL ++K  + ++F   Y 
Sbjct: 49  SVVELLGKSYPQDD------HSNLTRKVLTRVGRNLHNQQHHPLWLIKERVKEHFYKQYV 108

Query: 116 NK-----FNKFDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAE 175
            +     F+ +D+LSP+V+  +NFD +L+P DH SR   D YY++   +LR HTSAHQ +
Sbjct: 109 GRFGTPLFSVYDNLSPVVTTWQNFDSLLIPADHPSRKKGDNYYLNRTHMLRAHTSAHQWD 168

Query: 176 LLRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTS-------- 235
           LL  G   FLV GDVYRRD IDS HYP+FHQ+EAVR+FS ++  A   DG S        
Sbjct: 169 LLHAGLDAFLVVGDVYRRDQIDSQHYPIFHQLEAVRLFSKHELFAGIKDGESLQLFEQSS 228

Query: 236 ---------------YAADDLKKCLEGLARHLFG-AVEMRWVDAYFPFTNPSFELEIFFQ 295
                              DLK+ L  L  HLFG  +E+RWVD YFPFT+PSFE+EI F 
Sbjct: 229 RSAHKQETHTMEAVKLVEFDLKQTLTRLMAHLFGDELEIRWVDCYFPFTHPSFEMEINFH 288

Query: 296 EKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGLERLAMVLFDIPDVRLFWSADERFTSQ 355
            +WLEVLGCGVMEQ+++   G ++ + WAFGLGLERLAM+L+DIPD+RLFW  DERF  Q
Sbjct: 289 GEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQ 348

Query: 356 FSKGKLG--VKFKPFSKFPPCYKDISFWI-NDSFTENNLCEVVRGIAGDLVEEVQLIDSF 415
           F    +   VKF+P SK+P    DISFW+ ++++ EN+  ++VR I GDLVE+V LID F
Sbjct: 349 FCVSNINQKVKFQPLSKYPAVINDISFWLPSENYAENDFYDLVRTIGGDLVEKVDLIDKF 408

Query: 416 TNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVREQVQSRLNV 429
            + K   TSHCYRI YR MER+L+  E+  + + ++E     L V
Sbjct: 409 VHPKTHKTSHCYRITYRHMERTLSQREVRHIHQALQEAAVQLLGV 447

BLAST of Bhi03G000203 vs. ExPASy Swiss-Prot
Match: Q6AYQ3 (Phenylalanine--tRNA ligase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fars2 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 6.8e-99
Identity = 190/425 (44.71%), Postives = 256/425 (60.24%), Query Frame = 0

Query: 57  LLELGGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSN 116
           +LEL G +  +DD        N+   + SK+G  LH +  HPL ++K  + ++F   Y  
Sbjct: 50  VLELLGKSYPQDD------HTNLTQKVLSKVGRNLHNQKFHPLWLIKERVKEHFYQQYMG 109

Query: 117 K-----FNKFDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAEL 176
           +     F+ +D LSP+V+  +NFD +L+P DH SR   D YY++   +LR HTSAHQ +L
Sbjct: 110 RSRTPLFSVYDQLSPVVTTWQNFDSLLIPADHPSRKKGDNYYLNRGHMLRAHTSAHQWDL 169

Query: 177 LRRGYTQFLVTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTS--------- 236
           L  G   FLV GDVYRRD IDS HYPVFHQ+E VR+FS ++  A   DG S         
Sbjct: 170 LHAGLNAFLVVGDVYRRDQIDSQHYPVFHQLEGVRLFSKHELFAGVKDGESLQLFEESSR 229

Query: 237 --------------YAADDLKKCLEGLARHLFG-AVEMRWVDAYFPFTNPSFELEIFFQE 296
                             DLK+ L  L  HLFG  +E+RWVD YFPFT+PSFE+EI F+ 
Sbjct: 230 SAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEINFRG 289

Query: 297 KWLEVLGCGVMEQEILQKCGKENHVAWAFGLGLERLAMVLFDIPDVRLFWSADERFTSQF 356
           +WLEVLGCGVMEQ+++   G ++ + WAFGLGLERLAMVL+DIPD+RLFWS DERF  QF
Sbjct: 290 EWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMVLYDIPDIRLFWSEDERFLKQF 349

Query: 357 S-----------------------KGKLGVKFKPFSKFPPCYKDISFWI-NDSFTENNLC 416
                                   + K+G +  PFSK+P  + DISFW+ ++++TEN+  
Sbjct: 350 RLSDINQSVKFQRWFFQEERATGIQRKMGRQLCPFSKYPAVFNDISFWLPSENYTENDFY 409

Query: 417 EVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVREQVQ 429
           ++VR + GDLVE+V LID F + K   TSHCYRI YR MER+L+  E++ + + V+E   
Sbjct: 410 DIVRTVGGDLVEKVDLIDKFEHPKTHRTSHCYRITYRHMERTLSQREVSSVHQAVQEAAV 468

BLAST of Bhi03G000203 vs. NCBI nr
Match: XP_038883400.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida] >XP_038883401.1 phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida] >XP_038883402.1 phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 877.9 bits (2267), Expect = 3.7e-251
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL
Sbjct: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK
Sbjct: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL
Sbjct: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN
Sbjct: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQSRLNVVLR
Sbjct: 421 QVQSRLNVVLR 431

BLAST of Bhi03G000203 vs. NCBI nr
Match: XP_008439478.1 (PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo] >ADN34214.1 phenylalanyl-tRNA synthetase [Cucumis melo subsp. melo] >KAA0052439.1 phenylalanine--tRNA ligase [Cucumis melo var. makuwa] >TYK13383.1 phenylalanine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 836.3 bits (2159), Expect = 1.2e-238
Identity = 412/431 (95.59%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTLSLTQALLFT ASNLLRRN LRT VFSASFSSSSALSSD++HRKKW QPVSSLLEL
Sbjct: 1   MASTLSLTQALLFTTASNLLRRNSLRTTVFSASFSSSSALSSDSLHRKKWAQPVSSLLEL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+AIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIY YFD NYSNKF K
Sbjct: 61  GGVAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFIK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLND E SGTDGTSYAADDLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDLEVSGTDGTSYAADDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVDAYFPFT+PSFELEIFFQEKWLEVLGCGVMEQ+IL+KCGKENHVAWAFGLGL
Sbjct: 241 GAVEMRWVDAYFPFTDPSFELEIFFQEKWLEVLGCGVMEQKILKKCGKENHVAWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLF IPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWIN+SFTEN
Sbjct: 301 ERLAMVLFGIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQS+L VVLR
Sbjct: 421 QVQSKLKVVLR 431

BLAST of Bhi03G000203 vs. NCBI nr
Match: XP_004134536.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >XP_011658342.1 phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >XP_011658343.1 phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN49513.1 hypothetical protein Csa_004017 [Cucumis sativus])

HSP 1 Score: 827.8 bits (2137), Expect = 4.4e-236
Identity = 406/431 (94.20%), Postives = 417/431 (96.75%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTLSLTQALLFT ASNLLRRNCLRT V  ASFSSSSA+SSD++HRKKW QPVSSLLEL
Sbjct: 1   MASTLSLTQALLFTTASNLLRRNCLRTSVLYASFSSSSAISSDSLHRKKWTQPVSSLLEL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+AI KDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIY YFD NYSNKF K
Sbjct: 61  GGVAITKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFIK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQMEAVRVFSL+D E SGTDGTSYAADDLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLDDLEVSGTDGTSYAADDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVDAYFPFT+PSFELEIFFQEKWLEVLGCGVMEQ+IL+KCGKENHV+WAFGLGL
Sbjct: 241 GAVEMRWVDAYFPFTDPSFELEIFFQEKWLEVLGCGVMEQKILKKCGKENHVSWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLF IPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWIN+SFTEN
Sbjct: 301 ERLAMVLFGIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLIDSFTNEKKG TSHCYRIAYRSMERSLTDEEINDLQ NVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGTTSHCYRIAYRSMERSLTDEEINDLQTNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQS+LNVVLR
Sbjct: 421 QVQSKLNVVLR 431

BLAST of Bhi03G000203 vs. NCBI nr
Match: XP_022146633.1 (phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] >XP_022146634.1 phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 807.4 bits (2084), Expect = 6.1e-230
Identity = 395/431 (91.65%), Postives = 410/431 (95.13%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTL LT+A+LFTNAS LLRRNCLR F FSASFS     SSD VHRKKWRQPVSSLL+L
Sbjct: 1   MASTLPLTRAILFTNASILLRRNCLRNFAFSASFS-----SSDTVHRKKWRQPVSSLLQL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+ IAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIY YFD NYSNKF+K
Sbjct: 61  GGVVIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFDK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVP DHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRG TQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGNTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQME VRV S++DWEASGTDGTSYAA+DLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEGVRVVSVDDWEASGTDGTSYAANDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVD YFPFTNPSFELEIFFQEKWLEVLGCGVMEQEIL + GK N+VAWAFGLGL
Sbjct: 241 GAVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILTRSGKTNNVAWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLFDIPD+RLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWIN+SFTEN
Sbjct: 301 ERLAMVLFDIPDIRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLID+FTN+KKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDNFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQS+LNVVLR
Sbjct: 421 QVQSKLNVVLR 426

BLAST of Bhi03G000203 vs. NCBI nr
Match: KAG6581513.1 (Phenylalanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 806.2 bits (2081), Expect = 1.4e-229
Identity = 394/431 (91.42%), Postives = 409/431 (94.90%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           M STLSLT+  LFTNASNLLRRNCL TF FSASF      SSD++H+KKWRQPVSSLLEL
Sbjct: 40  MTSTLSLTRPFLFTNASNLLRRNCLATFSFSASF------SSDSLHQKKWRQPVSSLLEL 99

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+AI K DVVRDDPTNNVPD+IFSKLGMQLH+RDNHPLGILKNEIY YFD NYSNKF K
Sbjct: 100 GGVAIGKHDVVRDDPTNNVPDSIFSKLGMQLHQRDNHPLGILKNEIYNYFDTNYSNKFTK 159

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 160 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 219

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQME VRVFSLNDWEASGTDGTS+AADDLKKCLEGLARHLF
Sbjct: 220 VTGDVYRRDSIDSTHYPVFHQMEGVRVFSLNDWEASGTDGTSFAADDLKKCLEGLARHLF 279

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVD YFPFTNPSFELEIFFQEKWLEVLGCGVMEQEIL++  KEN+VAWAFGLGL
Sbjct: 280 GAVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILKRSDKENNVAWAFGLGL 339

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLFDIPD+RLFWSADERFTSQFSKGKLGVKFK FSKFPPCYKDISFWINDSFTEN
Sbjct: 340 ERLAMVLFDIPDIRLFWSADERFTSQFSKGKLGVKFKSFSKFPPCYKDISFWINDSFTEN 399

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRG+AGDLVEEVQLIDSFTN+KKGMTSHCYRIAYRSMERSLTDEEINDLQ NVRE
Sbjct: 400 NLCEVVRGVAGDLVEEVQLIDSFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQWNVRE 459

Query: 421 QVQSRLNVVLR 432
           QVQS+LNVVLR
Sbjct: 460 QVQSKLNVVLR 464

BLAST of Bhi03G000203 vs. ExPASy TrEMBL
Match: A0A5D3CN83 (Phenylalanyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G0010110 PE=3 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 5.9e-239
Identity = 412/431 (95.59%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTLSLTQALLFT ASNLLRRN LRT VFSASFSSSSALSSD++HRKKW QPVSSLLEL
Sbjct: 1   MASTLSLTQALLFTTASNLLRRNSLRTTVFSASFSSSSALSSDSLHRKKWAQPVSSLLEL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+AIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIY YFD NYSNKF K
Sbjct: 61  GGVAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFIK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLND E SGTDGTSYAADDLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDLEVSGTDGTSYAADDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVDAYFPFT+PSFELEIFFQEKWLEVLGCGVMEQ+IL+KCGKENHVAWAFGLGL
Sbjct: 241 GAVEMRWVDAYFPFTDPSFELEIFFQEKWLEVLGCGVMEQKILKKCGKENHVAWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLF IPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWIN+SFTEN
Sbjct: 301 ERLAMVLFGIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQS+L VVLR
Sbjct: 421 QVQSKLKVVLR 431

BLAST of Bhi03G000203 vs. ExPASy TrEMBL
Match: E5GCL5 (Phenylalanyl-tRNA synthetase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 5.9e-239
Identity = 412/431 (95.59%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTLSLTQALLFT ASNLLRRN LRT VFSASFSSSSALSSD++HRKKW QPVSSLLEL
Sbjct: 1   MASTLSLTQALLFTTASNLLRRNSLRTTVFSASFSSSSALSSDSLHRKKWAQPVSSLLEL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+AIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIY YFD NYSNKF K
Sbjct: 61  GGVAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFIK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLND E SGTDGTSYAADDLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDLEVSGTDGTSYAADDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVDAYFPFT+PSFELEIFFQEKWLEVLGCGVMEQ+IL+KCGKENHVAWAFGLGL
Sbjct: 241 GAVEMRWVDAYFPFTDPSFELEIFFQEKWLEVLGCGVMEQKILKKCGKENHVAWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLF IPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWIN+SFTEN
Sbjct: 301 ERLAMVLFGIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQS+L VVLR
Sbjct: 421 QVQSKLKVVLR 431

BLAST of Bhi03G000203 vs. ExPASy TrEMBL
Match: A0A1S3AYV5 (Phenylalanyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103484266 PE=3 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 5.9e-239
Identity = 412/431 (95.59%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTLSLTQALLFT ASNLLRRN LRT VFSASFSSSSALSSD++HRKKW QPVSSLLEL
Sbjct: 1   MASTLSLTQALLFTTASNLLRRNSLRTTVFSASFSSSSALSSDSLHRKKWAQPVSSLLEL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+AIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIY YFD NYSNKF K
Sbjct: 61  GGVAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFIK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLND E SGTDGTSYAADDLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDLEVSGTDGTSYAADDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVDAYFPFT+PSFELEIFFQEKWLEVLGCGVMEQ+IL+KCGKENHVAWAFGLGL
Sbjct: 241 GAVEMRWVDAYFPFTDPSFELEIFFQEKWLEVLGCGVMEQKILKKCGKENHVAWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLF IPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWIN+SFTEN
Sbjct: 301 ERLAMVLFGIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQS+L VVLR
Sbjct: 421 QVQSKLKVVLR 431

BLAST of Bhi03G000203 vs. ExPASy TrEMBL
Match: A0A0A0KJ29 (Phenylalanyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_6G526470 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.1e-236
Identity = 406/431 (94.20%), Postives = 417/431 (96.75%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTLSLTQALLFT ASNLLRRNCLRT V  ASFSSSSA+SSD++HRKKW QPVSSLLEL
Sbjct: 1   MASTLSLTQALLFTTASNLLRRNCLRTSVLYASFSSSSAISSDSLHRKKWTQPVSSLLEL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+AI KDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIY YFD NYSNKF K
Sbjct: 61  GGVAITKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFIK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQMEAVRVFSL+D E SGTDGTSYAADDLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLDDLEVSGTDGTSYAADDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVDAYFPFT+PSFELEIFFQEKWLEVLGCGVMEQ+IL+KCGKENHV+WAFGLGL
Sbjct: 241 GAVEMRWVDAYFPFTDPSFELEIFFQEKWLEVLGCGVMEQKILKKCGKENHVSWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLF IPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWIN+SFTEN
Sbjct: 301 ERLAMVLFGIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLIDSFTNEKKG TSHCYRIAYRSMERSLTDEEINDLQ NVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGTTSHCYRIAYRSMERSLTDEEINDLQTNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQS+LNVVLR
Sbjct: 421 QVQSKLNVVLR 431

BLAST of Bhi03G000203 vs. ExPASy TrEMBL
Match: A0A6J1CZ26 (Phenylalanyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015788 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 3.0e-230
Identity = 395/431 (91.65%), Postives = 410/431 (95.13%), Query Frame = 0

Query: 1   MASTLSLTQALLFTNASNLLRRNCLRTFVFSASFSSSSALSSDNVHRKKWRQPVSSLLEL 60
           MASTL LT+A+LFTNAS LLRRNCLR F FSASFS     SSD VHRKKWRQPVSSLL+L
Sbjct: 1   MASTLPLTRAILFTNASILLRRNCLRNFAFSASFS-----SSDTVHRKKWRQPVSSLLQL 60

Query: 61  GGIAIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYKYFDINYSNKFNK 120
           GG+ IAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIY YFD NYSNKF+K
Sbjct: 61  GGVVIAKDDVVRDDPTNNVPDNIFSKLGMQLHRRDNHPLGILKNEIYNYFDTNYSNKFDK 120

Query: 121 FDDLSPIVSVKENFDDVLVPEDHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGYTQFL 180
           FDDLSPIVSVKENFDDVLVP DHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRG TQFL
Sbjct: 121 FDDLSPIVSVKENFDDVLVPADHVSRSYNDTYYIDSQTVLRCHTSAHQAELLRRGNTQFL 180

Query: 181 VTGDVYRRDSIDSTHYPVFHQMEAVRVFSLNDWEASGTDGTSYAADDLKKCLEGLARHLF 240
           VTGDVYRRDSIDSTHYPVFHQME VRV S++DWEASGTDGTSYAA+DLKKCLEGLARHLF
Sbjct: 181 VTGDVYRRDSIDSTHYPVFHQMEGVRVVSVDDWEASGTDGTSYAANDLKKCLEGLARHLF 240

Query: 241 GAVEMRWVDAYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILQKCGKENHVAWAFGLGL 300
           GAVEMRWVD YFPFTNPSFELEIFFQEKWLEVLGCGVMEQEIL + GK N+VAWAFGLGL
Sbjct: 241 GAVEMRWVDTYFPFTNPSFELEIFFQEKWLEVLGCGVMEQEILTRSGKTNNVAWAFGLGL 300

Query: 301 ERLAMVLFDIPDVRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINDSFTEN 360
           ERLAMVLFDIPD+RLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWIN+SFTEN
Sbjct: 301 ERLAMVLFDIPDIRLFWSADERFTSQFSKGKLGVKFKPFSKFPPCYKDISFWINESFTEN 360

Query: 361 NLCEVVRGIAGDLVEEVQLIDSFTNEKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420
           NLCEVVRGIAGDLVEEVQLID+FTN+KKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE
Sbjct: 361 NLCEVVRGIAGDLVEEVQLIDNFTNKKKGMTSHCYRIAYRSMERSLTDEEINDLQRNVRE 420

Query: 421 QVQSRLNVVLR 432
           QVQS+LNVVLR
Sbjct: 421 QVQSKLNVVLR 426

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G58140.14.1e-19275.35phenylalanyl-tRNA synthetase class IIc family protein [more]
AT4G39280.17.2e-1127.50phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative [more]
AT4G39280.27.2e-1127.50phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative [more]
Match NameE-valueIdentityDescription
Q94K735.8e-19175.35Phenylalanine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana ... [more]
Q86A686.4e-10549.04Phenylalanine--tRNA ligase, mitochondrial OS=Dictyostelium discoideum OX=44689 G... [more]
Q99M013.6e-10046.67Phenylalanine--tRNA ligase, mitochondrial OS=Mus musculus OX=10090 GN=Fars2 PE=1... [more]
O953634.0e-9945.68Phenylalanine--tRNA ligase, mitochondrial OS=Homo sapiens OX=9606 GN=FARS2 PE=1 ... [more]
Q6AYQ36.8e-9944.71Phenylalanine--tRNA ligase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fars2... [more]
Match NameE-valueIdentityDescription
XP_038883400.13.7e-251100.00phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida] >XP_... [more]
XP_008439478.11.2e-23895.59PREDICTED: phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo... [more]
XP_004134536.14.4e-23694.20phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >XP_01... [more]
XP_022146633.16.1e-23091.65phenylalanine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] >X... [more]
KAG6581513.11.4e-22991.42Phenylalanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argy... [more]
Match NameE-valueIdentityDescription
A0A5D3CN835.9e-23995.59Phenylalanyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
E5GCL55.9e-23995.59Phenylalanyl-tRNA synthetase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3AYV55.9e-23995.59Phenylalanyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103484266 PE=3 SV=1[more]
A0A0A0KJ292.1e-23694.20Phenylalanyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_6G526470 PE=3 SV=... [more]
A0A6J1CZ263.0e-23091.65Phenylalanyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015788 PE=3... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005121Ferrodoxin-fold anticodon-binding domainSMARTSM00896FDX_ACB_2coord: 340..431
e-value: 5.7E-34
score: 128.9
IPR005121Ferrodoxin-fold anticodon-binding domainPFAMPF03147FDX-ACBcoord: 340..431
e-value: 1.2E-26
score: 92.9
IPR005121Ferrodoxin-fold anticodon-binding domainPROSITEPS51447FDX_ACBcoord: 340..431
score: 26.03619
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 48..330
e-value: 9.7E-97
score: 326.0
NoneNo IPR availablePANTHERPTHR11538PHENYLALANYL-TRNA SYNTHETASEcoord: 27..426
NoneNo IPR availableCDDcd00496PheRS_alpha_corecoord: 97..322
e-value: 1.79313E-93
score: 279.045
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 87..327
IPR036690Ferrodoxin-fold anticodon-binding domain superfamilyGENE3D3.30.70.380coord: 336..430
e-value: 8.1E-35
score: 120.7
IPR036690Ferrodoxin-fold anticodon-binding domain superfamilySUPERFAMILY54991Anticodon-binding domain of PheRScoord: 335..431
IPR002319Phenylalanyl-tRNA synthetasePFAMPF01409tRNA-synt_2dcoord: 93..327
e-value: 3.2E-50
score: 170.9
IPR004530Phenylalanyl-tRNA synthetase, class IIc, mitochondrialTIGRFAMTIGR00469TIGR00469coord: 242..428
e-value: 1.2E-79
score: 266.1
IPR004530Phenylalanyl-tRNA synthetase, class IIc, mitochondrialPANTHERPTHR11538:SF41PHENYLALANINE--TRNA LIGASE, MITOCHONDRIALcoord: 27..426
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 183..338
score: 12.18001

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000203Bhi03M000203mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006432 phenylalanyl-tRNA aminoacylation
biological_process GO:0043039 tRNA aminoacylation
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004826 phenylalanine-tRNA ligase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding