Bhi03G000165 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000165
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionFAD-binding Berberine family protein
Locationchr3: 4290100 .. 4292171 (-)
RNA-Seq ExpressionBhi03G000165
SyntenyBhi03G000165
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTATCTATAAATGAGAAAGGACAGATATTACTTAAGCTTCAAGTATGAAGTGCCAGCCTCCAATGGTCAATGGAAATGCCTGGTTGTTTCTTTTGTTTTCGTCATCTTCCTCCCAACGCCATTTCAATGGGGATTCCAAATTCATTATTAATCCCAGTTCTCACAACCTTTCTTCTCATTCTCCCTTCAATGGCGGTTTCTGATCAAATCCCATCTCATCAAACCCTTCTCAAGTGTCTTTCCGATCATTCTGCGCCATCATCTTCTCCAATCTCTGAAGTTATCTTCTTCCCCGATAACCCATCTTACTCCCCTGTCTTAAACTCTTACATCAGAAATCTCAGGTTCGCCTCACCCACTACCCCCAAACCCTTGTTCATCGTCGCCCCAACCCATGTCTCTCACATTCAAGCCACGATTCTCTGTTGCAGAATCCACGCCCTAGAAATCAGAATCCGAAGTGGTGGCCATGATTACGACGGTCTCTCTTATGTCTCCGATTCCACATTTGTGATCCTCGATATGTTCAATCTTCGATCTGTAGCTGTCGACATTGAAGACGAGAGCGCGTGGGTGGATTCGGGAGCAACCCTTGGCGAAGTTTACTTCAAAATTGCAGAGAAGAGCAAGATCCATGGATTCCCAGCTGGGGTTTGCCCCACGGTTGGAGTTGGAGGACATCTCAGCGGCGCCGGATACGGTAATTTGATGAGAAAATTTGGGGTTTCTGTGGACAATGTAGTCGATGCTTTAATTGTTGATGTAAATGGTAGGGTTTTGGATAGAGCATCAATGGGAGAAGATTTATTTTGGGCAATTAGAGGAGGAGGTGGAGCTAGTTTTGGTGTCATTGTTTCGTGGAAGTTTAAACTTGTTCCTTTACCTGAAACTGTCACTGTTTTTAGAACGGAGAAAACCATGGAGGAAGGTGTTGTAGATATCTTGTACAAATGGCAAGAAATTGCTGATAAAATTGATGAAAATCTATTCATAAGAGTGGTGATTCTTCCTGTAAATAAAAAAGCCCTAAAGACAGCAAAAGCCAAGTTCATTTCCTTGTTCCTTGGAAATGCACAGAAGCTGTTTGCGTTAATGTCTAAAAAGTTGCCGGAATTGGGTATTAAGGTTGAGGATTGTATAGAAATGAGCTGGATTGACTCTGTTCTGTTCTGGTCAAACTACCCAATTGGAACTCCACTCAATGTCTTGCTAGAAAGGCAACCAAAATCTGAAAAGTTCTTGAAGAAGAAATCAGATTATGTTCAAGAACCAATCTCAAAGGCAGACCTTGAAGGAATGATGAGGAAAATGATTGAACTGAAAAGACCAGCGCTGACTTTCAATCCATATGGTGGGAAAATGAGTCAAATTCCAGAGACGGAGACTCCATTTCCACATAGAGCAGGGAACAAATACAAAATACAATACTCAGTGACATGGAAAGAAGAAGGAGATGAGGCTGCAGACAAGAATGTGGAGCTGATCAGAGAGATGTATGAGTACATGACACCGTACGTTTCGAAATCGCCTAGAAGTGCTTATTTGAACTACAGAGATGTGGATTTGGGAGTGAATGGGAATGGGCATGCAAGCAGTTGGGGAAGAAAGTACTTCAAAGGAAACTTTGATAGATTGGTGAAGGTGAAGAGCATGGTGGATCCAGATAACTTTTTCAGATATGAACAGAGCATCCCATGTGTTGAAGCATTTGATTCTAATGGCAGAGCTGAGAGCCTTTGAAGTTTGAAGTGTGCATTGCTTGGTTGAAAATTGAAATTCATTCCCATATTATTTCTGGGATTTGTGTAATGAAAAGCTTAATTATCCCACTGATTAATGTTGTATTTGTAATTTGTTAATTGTTTTACCTATAAAATAATAATAACAATTAGAAAGAGAAATGTTAAAGGTTGCATTTTTCTTTCATGAATGGGCCATACCTTTGGACTTTTGGACTGATCTTGTAAGAGCCCAAATCTATCTTTTGTAAGAAAAATGGATTGGTTTATTACAATTGCACCCAATAAATTTTAATTTAATCAATGACAGCTCTAAATTATATGATTTGCT

mRNA sequence

CTTTATCTATAAATGAGAAAGGACAGATATTACTTAAGCTTCAAGTATGAAGTGCCAGCCTCCAATGGTCAATGGAAATGCCTGGTTGTTTCTTTTGTTTTCGTCATCTTCCTCCCAACGCCATTTCAATGGGGATTCCAAATTCATTATTAATCCCAGTTCTCACAACCTTTCTTCTCATTCTCCCTTCAATGGCGGTTTCTGATCAAATCCCATCTCATCAAACCCTTCTCAAGTGTCTTTCCGATCATTCTGCGCCATCATCTTCTCCAATCTCTGAAGTTATCTTCTTCCCCGATAACCCATCTTACTCCCCTGTCTTAAACTCTTACATCAGAAATCTCAGGTTCGCCTCACCCACTACCCCCAAACCCTTGTTCATCGTCGCCCCAACCCATGTCTCTCACATTCAAGCCACGATTCTCTGTTGCAGAATCCACGCCCTAGAAATCAGAATCCGAAGTGGTGGCCATGATTACGACGGTCTCTCTTATGTCTCCGATTCCACATTTGTGATCCTCGATATGTTCAATCTTCGATCTGTAGCTGTCGACATTGAAGACGAGAGCGCGTGGGTGGATTCGGGAGCAACCCTTGGCGAAGTTTACTTCAAAATTGCAGAGAAGAGCAAGATCCATGGATTCCCAGCTGGGGTTTGCCCCACGGTTGGAGTTGGAGGACATCTCAGCGGCGCCGGATACGGTAATTTGATGAGAAAATTTGGGGTTTCTGTGGACAATGTAGTCGATGCTTTAATTGTTGATGTAAATGGTAGGGTTTTGGATAGAGCATCAATGGGAGAAGATTTATTTTGGGCAATTAGAGGAGGAGGTGGAGCTAGTTTTGGTGTCATTGTTTCGTGGAAGTTTAAACTTGTTCCTTTACCTGAAACTGTCACTGTTTTTAGAACGGAGAAAACCATGGAGGAAGGTGTTGTAGATATCTTGTACAAATGGCAAGAAATTGCTGATAAAATTGATGAAAATCTATTCATAAGAGTGGTGATTCTTCCTGTAAATAAAAAAGCCCTAAAGACAGCAAAAGCCAAGTTCATTTCCTTGTTCCTTGGAAATGCACAGAAGCTGTTTGCGTTAATGTCTAAAAAGTTGCCGGAATTGGGTATTAAGGTTGAGGATTGTATAGAAATGAGCTGGATTGACTCTGTTCTGTTCTGGTCAAACTACCCAATTGGAACTCCACTCAATGTCTTGCTAGAAAGGCAACCAAAATCTGAAAAGTTCTTGAAGAAGAAATCAGATTATGTTCAAGAACCAATCTCAAAGGCAGACCTTGAAGGAATGATGAGGAAAATGATTGAACTGAAAAGACCAGCGCTGACTTTCAATCCATATGGTGGGAAAATGAGTCAAATTCCAGAGACGGAGACTCCATTTCCACATAGAGCAGGGAACAAATACAAAATACAATACTCAGTGACATGGAAAGAAGAAGGAGATGAGGCTGCAGACAAGAATGTGGAGCTGATCAGAGAGATGTATGAGTACATGACACCGTACGTTTCGAAATCGCCTAGAAGTGCTTATTTGAACTACAGAGATGTGGATTTGGGAGTGAATGGGAATGGGCATGCAAGCAGTTGGGGAAGAAAGTACTTCAAAGGAAACTTTGATAGATTGGTGAAGGTGAAGAGCATGGTGGATCCAGATAACTTTTTCAGATATGAACAGAGCATCCCATGTGTTGAAGCATTTGATTCTAATGGCAGAGCTGAGAGCCTTTGAAGTTTGAAGTGTGCATTGCTTGGTTGAAAATTGAAATTCATTCCCATATTATTTCTGGGATTTGTGTAATGAAAAGCTTAATTATCCCACTGATTAATGTTGTATTTGTAATTTGTTAATTGTTTTACCTATAAAATAATAATAACAATTAGAAAGAGAAATGTTAAAGGTTGCATTTTTCTTTCATGAATGGGCCATACCTTTGGACTTTTGGACTGATCTTGTAAGAGCCCAAATCTATCTTTTGTAAGAAAAATGGATTGGTTTATTACAATTGCACCCAATAAATTTTAATTTAATCAATGACAGCTCTAAATTATATGATTTGCT

Coding sequence (CDS)

ATGGAAATGCCTGGTTGTTTCTTTTGTTTTCGTCATCTTCCTCCCAACGCCATTTCAATGGGGATTCCAAATTCATTATTAATCCCAGTTCTCACAACCTTTCTTCTCATTCTCCCTTCAATGGCGGTTTCTGATCAAATCCCATCTCATCAAACCCTTCTCAAGTGTCTTTCCGATCATTCTGCGCCATCATCTTCTCCAATCTCTGAAGTTATCTTCTTCCCCGATAACCCATCTTACTCCCCTGTCTTAAACTCTTACATCAGAAATCTCAGGTTCGCCTCACCCACTACCCCCAAACCCTTGTTCATCGTCGCCCCAACCCATGTCTCTCACATTCAAGCCACGATTCTCTGTTGCAGAATCCACGCCCTAGAAATCAGAATCCGAAGTGGTGGCCATGATTACGACGGTCTCTCTTATGTCTCCGATTCCACATTTGTGATCCTCGATATGTTCAATCTTCGATCTGTAGCTGTCGACATTGAAGACGAGAGCGCGTGGGTGGATTCGGGAGCAACCCTTGGCGAAGTTTACTTCAAAATTGCAGAGAAGAGCAAGATCCATGGATTCCCAGCTGGGGTTTGCCCCACGGTTGGAGTTGGAGGACATCTCAGCGGCGCCGGATACGGTAATTTGATGAGAAAATTTGGGGTTTCTGTGGACAATGTAGTCGATGCTTTAATTGTTGATGTAAATGGTAGGGTTTTGGATAGAGCATCAATGGGAGAAGATTTATTTTGGGCAATTAGAGGAGGAGGTGGAGCTAGTTTTGGTGTCATTGTTTCGTGGAAGTTTAAACTTGTTCCTTTACCTGAAACTGTCACTGTTTTTAGAACGGAGAAAACCATGGAGGAAGGTGTTGTAGATATCTTGTACAAATGGCAAGAAATTGCTGATAAAATTGATGAAAATCTATTCATAAGAGTGGTGATTCTTCCTGTAAATAAAAAAGCCCTAAAGACAGCAAAAGCCAAGTTCATTTCCTTGTTCCTTGGAAATGCACAGAAGCTGTTTGCGTTAATGTCTAAAAAGTTGCCGGAATTGGGTATTAAGGTTGAGGATTGTATAGAAATGAGCTGGATTGACTCTGTTCTGTTCTGGTCAAACTACCCAATTGGAACTCCACTCAATGTCTTGCTAGAAAGGCAACCAAAATCTGAAAAGTTCTTGAAGAAGAAATCAGATTATGTTCAAGAACCAATCTCAAAGGCAGACCTTGAAGGAATGATGAGGAAAATGATTGAACTGAAAAGACCAGCGCTGACTTTCAATCCATATGGTGGGAAAATGAGTCAAATTCCAGAGACGGAGACTCCATTTCCACATAGAGCAGGGAACAAATACAAAATACAATACTCAGTGACATGGAAAGAAGAAGGAGATGAGGCTGCAGACAAGAATGTGGAGCTGATCAGAGAGATGTATGAGTACATGACACCGTACGTTTCGAAATCGCCTAGAAGTGCTTATTTGAACTACAGAGATGTGGATTTGGGAGTGAATGGGAATGGGCATGCAAGCAGTTGGGGAAGAAAGTACTTCAAAGGAAACTTTGATAGATTGGTGAAGGTGAAGAGCATGGTGGATCCAGATAACTTTTTCAGATATGAACAGAGCATCCCATGTGTTGAAGCATTTGATTCTAATGGCAGAGCTGAGAGCCTTTGA

Protein sequence

MEMPGCFFCFRHLPPNAISMGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGHASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFDSNGRAESL
Homology
BLAST of Bhi03G000165 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 568.5 bits (1464), Expect = 5.7e-162
Identity = 282/521 (54.13%), Postives = 370/521 (71.02%), Query Frame = 0

Query: 34  FLLILP---SMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRN 93
           FL+ LP   S A       +++ ++C SD +    + I++ +F   NPS+S VL +YIRN
Sbjct: 16  FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRN 75

Query: 94  LRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVIL 153
            RF + +TPKP  IV P    H+ A + C +     ++IRSGGHDY+GLSY+SD  F IL
Sbjct: 76  GRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFIL 135

Query: 154 DMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGY 213
           DM NLR V+VDI D+SAW+ +GATLGEVY++I EKSK+HGFPAGVCPTVGVGGH+SG GY
Sbjct: 136 DMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGY 195

Query: 214 GNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVP 273
           GN++RKFG+SVDN++DA IVDVNG++LDR SMGEDLFWAI GGGGASFGV++ +K KLVP
Sbjct: 196 GNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVP 255

Query: 274 LPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISL 333
           +PETVTVFR EK M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A  ++L
Sbjct: 256 VPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVAL 315

Query: 334 FLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLERQPKSE 393
           FLG A+++ AL+ K+ PEL +K E+C EM+W  S L+W N    T ++  V L+R     
Sbjct: 316 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 375

Query: 394 KFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNK 453
            F K+KSDYV   I +  +E + +KM EL +  L FNPYGGKM+++    TPFPHR+   
Sbjct: 376 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 435

Query: 454 YKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNG--- 513
           +KIQYSVTW+E   E     +     +Y +MT +VSK+PR+AYLNYRDVD+GVN +G   
Sbjct: 436 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 495

Query: 514 --HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCV 545
                 +GRKYF  NFDRLVKVK+  DPDNFFR EQSIP V
Sbjct: 496 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of Bhi03G000165 vs. TAIR 10
Match: AT1G34575.1 (FAD-binding Berberine family protein )

HSP 1 Score: 563.9 bits (1452), Expect = 1.4e-160
Identity = 281/521 (53.93%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 30  VLTTFLLILP-SMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYI 89
           + T FLL++P S A   ++    T  +C++      S PI    +   NP++  +LN+Y+
Sbjct: 10  IFTVFLLLIPTSFAAPPKLKDSFT--QCVTVFK--PSVPIQNFTYTQQNPNFLTILNNYV 69

Query: 90  RNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFV 149
           RNLR+ + TT KP+ IVA  H +HIQATI C +   L++RIRSGGHDYDG+SY+S   FV
Sbjct: 70  RNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLSTVDFV 129

Query: 150 ILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKS-KIHGFPAGVCPTVGVGGHLSG 209
           +LDMFNLR++ +D + ++AWV SGATLGE+Y+ +A KS  + GFPAG+CP +G GGH SG
Sbjct: 130 VLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGHFSG 189

Query: 210 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 269
            GYGN+MRK+G+S+DN++DA IVD N RVLDR+SMGEDLFWA+RGGG ASF V+++WK K
Sbjct: 190 GGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIK 249

Query: 270 LVPLPETVTVFRTEKTMEEGVV--DILYKWQEIADKIDENLFIRVVILPVNKKALKTAKA 329
           LVP+PE VTVF  E     GV+  D+  KWQEIADKID +LFIR+ +   N    KT KA
Sbjct: 250 LVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN----KTVKA 309

Query: 330 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGT-PLNVLLERQ 389
            F+ ++LGN++KL  +M+ K PELG+   +CIEM WI+SVLFW + P GT P +V+L R 
Sbjct: 310 SFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRI 369

Query: 390 PKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHR 449
           P+ + +LK+KSDYVQ+PISK  LE + + + E +  ++ +NPYGG+MS+IP TET FPHR
Sbjct: 370 PQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFPHR 429

Query: 450 AGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGN 509
           AGN +KIQYS  W   G+EAA   +     ++E M+PYVSK+PR A+LNYRD+D+G N N
Sbjct: 430 AGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDIGKNLN 489

Query: 510 G---HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIP 543
                   +G KYFK NF+RLV+VK+ VDPDN FRYEQSIP
Sbjct: 490 STYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of Bhi03G000165 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 563.5 bits (1451), Expect = 1.8e-160
Identity = 280/524 (53.44%), Postives = 379/524 (72.33%), Query Frame = 0

Query: 30  VLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIR 89
           +L  F ++    + S    S +T  +CL+ +S P   PIS  IFF  N SYS VL + IR
Sbjct: 6   ILVLFFVVFIWQSSSSSANS-ETFTQCLTSNSDP-KHPISPAIFFSGNGSYSSVLQANIR 65

Query: 90  NLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYV--SDSTF 149
           NLRF + +TPKP  I+A TH SH+QA I C + H L+++IRSGGHDYDGLSYV  S   F
Sbjct: 66  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 125

Query: 150 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSG 209
            +LDMFNLRSV VD+  ++AWV +GA LGEVY+ I EKSK   +PAG+CPTVGVGGH+SG
Sbjct: 126 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 185

Query: 210 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 269
            GYGN+MRK+G++VDN +DA +VDVNG++LDR  MGEDL+WAI GGGG S+GV++++K  
Sbjct: 186 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 245

Query: 270 LVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVN--KKALKTAKA 329
           LV +PE VTVFR  +T+E+   DI+++WQ++A K+ + LFIR VI  VN    + KT + 
Sbjct: 246 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 305

Query: 330 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQP 389
            FI++FLG+   L ++++++ PELG+   DC E SWI SVLFW+N  +G+   +LL+R  
Sbjct: 306 TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN- 365

Query: 390 KSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRA 449
           +   +LK+KSDYV+EPIS+  LE + +KMIEL+ P + FNPYGG+M +I  T TPFP+RA
Sbjct: 366 QPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRA 425

Query: 450 GNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNG-N 509
           GN +KIQY   W++E     D+ +EL R++Y++MTP+VSK+PR ++ NYRDVDLG+N  N
Sbjct: 426 GNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHN 485

Query: 510 GHASS------WGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIP 543
           G  SS      +G+KYF GNF+RLVK+K+ VD  NFFR EQSIP
Sbjct: 486 GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Bhi03G000165 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 560.1 bits (1442), Expect = 2.0e-159
Identity = 279/528 (52.84%), Postives = 366/528 (69.32%), Query Frame = 0

Query: 28  IPVLTTFLLI--LPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLN 87
           +P    FLL   LP  + S     + + LKC SD +    S I++ +F   NP++S VL 
Sbjct: 12  VPFFFFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLR 71

Query: 88  SYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDS 147
           +YIRN RF + +T KP  I+ P   SH+ A + C +     ++IRSGGHDYDGLSY+SD 
Sbjct: 72  AYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK 131

Query: 148 TFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHL 207
            F ILDM N+R V+VDI   SAW+ +GATLGEVY++I EKS++HGFPAGVCPTVGVGGHL
Sbjct: 132 PFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHL 191

Query: 208 SGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWK 267
           SG GYGN++RKFG+SVD V DA IVDVNGRVLDR +MGEDLFWAI GGGG S+GV++ +K
Sbjct: 192 SGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYK 251

Query: 268 FKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKA 327
            KLVP+P  VTVFR E+ M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A
Sbjct: 252 VKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRA 311

Query: 328 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLER 387
             ++LFLG A ++ AL+SK+ PELG+K E+C EM+W  S L+W N    T ++  V L+R
Sbjct: 312 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 371

Query: 388 QPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPH 447
              +  F K+KSDYV   I K  +E + +KMIEL +  L FNPYGGKM+++     PFPH
Sbjct: 372 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 431

Query: 448 RAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNG 507
           R    +KIQYSV WKE   E     +   + +Y +MT +VSK+PRS+Y NYRDVD+GVN 
Sbjct: 432 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 491

Query: 508 NG-----HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEA 547
           +G         +GRKYF  NFDRLVK+K+ VDP NFFR EQSIP +++
Sbjct: 492 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTLKS 538

BLAST of Bhi03G000165 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 559.7 bits (1441), Expect = 2.7e-159
Identity = 279/527 (52.94%), Postives = 365/527 (69.26%), Query Frame = 0

Query: 28  IPVLTTFLLI--LPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLN 87
           +P    FLL   LP  + S     + + LKC SD +    S I++ +F   NP++S VL 
Sbjct: 12  VPFFFFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLR 71

Query: 88  SYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDS 147
           +YIRN RF + +T KP  I+ P   SH+ A + C +     ++IRSGGHDYDGLSY+SD 
Sbjct: 72  AYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK 131

Query: 148 TFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHL 207
            F ILDM N+R V+VDI   SAW+ +GATLGEVY++I EKS++HGFPAGVCPTVGVGGHL
Sbjct: 132 PFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHL 191

Query: 208 SGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWK 267
           SG GYGN++RKFG+SVD V DA IVDVNGRVLDR +MGEDLFWAI GGGG S+GV++ +K
Sbjct: 192 SGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYK 251

Query: 268 FKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKA 327
            KLVP+P  VTVFR E+ M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A
Sbjct: 252 VKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRA 311

Query: 328 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLER 387
             ++LFLG A ++ AL+SK+ PELG+K E+C EM+W  S L+W N    T ++  V L+R
Sbjct: 312 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 371

Query: 388 QPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPH 447
              +  F K+KSDYV   I K  +E + +KMIEL +  L FNPYGGKM+++     PFPH
Sbjct: 372 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 431

Query: 448 RAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNG 507
           R    +KIQYSV WKE   E     +   + +Y +MT +VSK+PRS+Y NYRDVD+GVN 
Sbjct: 432 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 491

Query: 508 NG-----HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVE 546
           +G         +GRKYF  NFDRLVK+K+ VDP NFFR EQSIP ++
Sbjct: 492 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTLK 537

BLAST of Bhi03G000165 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 8.1e-161
Identity = 282/521 (54.13%), Postives = 370/521 (71.02%), Query Frame = 0

Query: 34  FLLILP---SMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIRN 93
           FL+ LP   S A       +++ ++C SD +    + I++ +F   NPS+S VL +YIRN
Sbjct: 16  FLVSLPFFSSAAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRN 75

Query: 94  LRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVIL 153
            RF + +TPKP  IV P    H+ A + C +     ++IRSGGHDY+GLSY+SD  F IL
Sbjct: 76  GRFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFIL 135

Query: 154 DMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGY 213
           DM NLR V+VDI D+SAW+ +GATLGEVY++I EKSK+HGFPAGVCPTVGVGGH+SG GY
Sbjct: 136 DMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGY 195

Query: 214 GNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVP 273
           GN++RKFG+SVDN++DA IVDVNG++LDR SMGEDLFWAI GGGGASFGV++ +K KLVP
Sbjct: 196 GNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVP 255

Query: 274 LPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISL 333
           +PETVTVFR EK M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A  ++L
Sbjct: 256 VPETVTVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVAL 315

Query: 334 FLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLERQPKSE 393
           FLG A+++ AL+ K+ PEL +K E+C EM+W  S L+W N    T ++  V L+R     
Sbjct: 316 FLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRA 375

Query: 394 KFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNK 453
            F K+KSDYV   I +  +E + +KM EL +  L FNPYGGKM+++    TPFPHR+   
Sbjct: 376 NFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS-KL 435

Query: 454 YKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNG--- 513
           +KIQYSVTW+E   E     +     +Y +MT +VSK+PR+AYLNYRDVD+GVN +G   
Sbjct: 436 FKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNS 495

Query: 514 --HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCV 545
                 +GRKYF  NFDRLVKVK+  DPDNFFR EQSIP V
Sbjct: 496 YEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTV 535

BLAST of Bhi03G000165 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 2.0e-159
Identity = 281/521 (53.93%), Postives = 375/521 (71.98%), Query Frame = 0

Query: 30  VLTTFLLILP-SMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYI 89
           + T FLL++P S A   ++    T  +C++      S PI    +   NP++  +LN+Y+
Sbjct: 10  IFTVFLLLIPTSFAAPPKLKDSFT--QCVTVFK--PSVPIQNFTYTQQNPNFLTILNNYV 69

Query: 90  RNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFV 149
           RNLR+ + TT KP+ IVA  H +HIQATI C +   L++RIRSGGHDYDG+SY+S   FV
Sbjct: 70  RNLRYFNGTTRKPVAIVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLSTVDFV 129

Query: 150 ILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKS-KIHGFPAGVCPTVGVGGHLSG 209
           +LDMFNLR++ +D + ++AWV SGATLGE+Y+ +A KS  + GFPAG+CP +G GGH SG
Sbjct: 130 VLDMFNLRAIEIDPKLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGHFSG 189

Query: 210 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 269
            GYGN+MRK+G+S+DN++DA IVD N RVLDR+SMGEDLFWA+RGGG ASF V+++WK K
Sbjct: 190 GGYGNMMRKYGLSIDNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIK 249

Query: 270 LVPLPETVTVFRTEKTMEEGVV--DILYKWQEIADKIDENLFIRVVILPVNKKALKTAKA 329
           LVP+PE VTVF  E     GV+  D+  KWQEIADKID +LFIR+ +   N    KT KA
Sbjct: 250 LVPVPEKVTVFNVETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN----KTVKA 309

Query: 330 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGT-PLNVLLERQ 389
            F+ ++LGN++KL  +M+ K PELG+   +CIEM WI+SVLFW + P GT P +V+L R 
Sbjct: 310 SFMGMYLGNSEKLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRI 369

Query: 390 PKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHR 449
           P+ + +LK+KSDYVQ+PISK  LE + + + E +  ++ +NPYGG+MS+IP TET FPHR
Sbjct: 370 PQKQIYLKRKSDYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFPHR 429

Query: 450 AGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGN 509
           AGN +KIQYS  W   G+EAA   +     ++E M+PYVSK+PR A+LNYRD+D+G N N
Sbjct: 430 AGNMFKIQYSSNWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDIGKNLN 489

Query: 510 G---HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIP 543
                   +G KYFK NF+RLV+VK+ VDPDN FRYEQSIP
Sbjct: 490 STYEEGKVYGVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP 522

BLAST of Bhi03G000165 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.6e-159
Identity = 280/524 (53.44%), Postives = 379/524 (72.33%), Query Frame = 0

Query: 30  VLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSYIR 89
           +L  F ++    + S    S +T  +CL+ +S P   PIS  IFF  N SYS VL + IR
Sbjct: 6   ILVLFFVVFIWQSSSSSANS-ETFTQCLTSNSDP-KHPISPAIFFSGNGSYSSVLQANIR 65

Query: 90  NLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYV--SDSTF 149
           NLRF + +TPKP  I+A TH SH+QA I C + H L+++IRSGGHDYDGLSYV  S   F
Sbjct: 66  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 125

Query: 150 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSG 209
            +LDMFNLRSV VD+  ++AWV +GA LGEVY+ I EKSK   +PAG+CPTVGVGGH+SG
Sbjct: 126 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 185

Query: 210 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 269
            GYGN+MRK+G++VDN +DA +VDVNG++LDR  MGEDL+WAI GGGG S+GV++++K  
Sbjct: 186 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 245

Query: 270 LVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVN--KKALKTAKA 329
           LV +PE VTVFR  +T+E+   DI+++WQ++A K+ + LFIR VI  VN    + KT + 
Sbjct: 246 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 305

Query: 330 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQP 389
            FI++FLG+   L ++++++ PELG+   DC E SWI SVLFW+N  +G+   +LL+R  
Sbjct: 306 TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN- 365

Query: 390 KSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRA 449
           +   +LK+KSDYV+EPIS+  LE + +KMIEL+ P + FNPYGG+M +I  T TPFP+RA
Sbjct: 366 QPVNYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRA 425

Query: 450 GNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNG-N 509
           GN +KIQY   W++E     D+ +EL R++Y++MTP+VSK+PR ++ NYRDVDLG+N  N
Sbjct: 426 GNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHN 485

Query: 510 GHASS------WGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIP 543
           G  SS      +G+KYF GNF+RLVK+K+ VD  NFFR EQSIP
Sbjct: 486 GKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Bhi03G000165 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 559.7 bits (1441), Expect = 3.7e-158
Identity = 279/527 (52.94%), Postives = 365/527 (69.26%), Query Frame = 0

Query: 28  IPVLTTFLLI--LPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLN 87
           +P    FLL   LP  + S     + + LKC SD +    S I++ +F   NP++S VL 
Sbjct: 12  VPFFFFFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLR 71

Query: 88  SYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDS 147
           +YIRN RF + +T KP  I+ P   SH+ A + C +     ++IRSGGHDYDGLSY+SD 
Sbjct: 72  AYIRNARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK 131

Query: 148 TFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHL 207
            F ILDM N+R V+VDI   SAW+ +GATLGEVY++I EKS++HGFPAGVCPTVGVGGHL
Sbjct: 132 PFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHL 191

Query: 208 SGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWK 267
           SG GYGN++RKFG+SVD V DA IVDVNGRVLDR +MGEDLFWAI GGGG S+GV++ +K
Sbjct: 192 SGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYK 251

Query: 268 FKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKA 327
            KLVP+P  VTVFR E+ M+ G VD+++KWQ +  K D NLF+R++I PV +K +KT +A
Sbjct: 252 VKLVPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRA 311

Query: 328 KFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLN--VLLER 387
             ++LFLG A ++ AL+SK+ PELG+K E+C EM+W  S L+W N    T ++  V L+R
Sbjct: 312 SVVALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDR 371

Query: 388 QPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPH 447
              +  F K+KSDYV   I K  +E + +KMIEL +  L FNPYGGKM+++     PFPH
Sbjct: 372 NLDTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPH 431

Query: 448 RAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNG 507
           R    +KIQYSV WKE   E     +   + +Y +MT +VSK+PRS+Y NYRDVD+GVN 
Sbjct: 432 R-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVND 491

Query: 508 NG-----HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVE 546
           +G         +GRKYF  NFDRLVK+K+ VDP NFFR EQSIP ++
Sbjct: 492 HGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTLK 537

BLAST of Bhi03G000165 vs. ExPASy Swiss-Prot
Match: Q9SA86 (Berberine bridge enzyme-like 9 OS=Arabidopsis thaliana OX=3702 GN=At1g30710 PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.3e-155
Identity = 277/527 (52.56%), Postives = 370/527 (70.21%), Query Frame = 0

Query: 30  VLTTFLLILPSMAVSDQIPS-HQTLLKCLSDHSAPS-SSPISEVIFFPDNPSYSPVLNSY 89
           ++T FLL +P+   S   PS      +C++D    +  SPI   I+   +P++  +LN+Y
Sbjct: 10  IITIFLLTIPTSFASP--PSLEDVFAQCVTDFKPSNPKSPIQNYIYTQRSPNFLTILNNY 69

Query: 90  IRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTF 149
           +RNLR+ +  T KP+ IVA   V+HIQATI C +   L++RIRSGGHDYDG+SY+S   F
Sbjct: 70  VRNLRYFNNMTRKPVAIVAAADVTHIQATITCAKKLGLQLRIRSGGHDYDGMSYLSTIDF 129

Query: 150 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSK-IHGFPAGVCPTVGVGGHLS 209
           V+LDMFNLRS+ +D + ++AWV SGATLGE+Y+ +A KS  + GFPAG+CP +G GGH S
Sbjct: 130 VVLDMFNLRSINIDPKLDTAWVQSGATLGEIYYGVANKSNDLRGFPAGICPGLGAGGHFS 189

Query: 210 GAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKF 269
           G GYGN+MRK+G+S+DN++DA IVD  GRVLDR+SMGEDLFWA+RGGG ASF V+++WK 
Sbjct: 190 GGGYGNMMRKYGLSIDNIIDAKIVDAKGRVLDRSSMGEDLFWALRGGGAASFCVVLAWKI 249

Query: 270 KLVPLPETVTVFRTEKTMEEGVV---DILYKWQEIADKIDENLFIRVVILPVNKKALKTA 329
           KLVP+P  VTVF  E     G V   +++ KWQEIADKID +LFIR+ +   N    KT 
Sbjct: 250 KLVPVPAKVTVFNIETFGNTGSVNTTELVAKWQEIADKIDNDLFIRLTLGSSN----KTV 309

Query: 330 KAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGT-PLNVLLE 389
           KA F+ ++LGN+  L  +M+ K PELG+   +CIEM WI+SVLFW   P GT P   +L 
Sbjct: 310 KASFMGMYLGNSSNLLEIMNAKFPELGLIKRECIEMKWIESVLFWLGIPPGTAPTTSMLN 369

Query: 390 RQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFP 449
           R P+ + +LK+KSDYVQ+PIS+  LE + + M E +   + FNPYGG+MS+IP TET FP
Sbjct: 370 RIPQKQIYLKRKSDYVQKPISRTGLESIFKIMTENENVTMAFNPYGGRMSEIPSTETAFP 429

Query: 450 HRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVN 509
           HRAGN +KIQY+  W   G+  A   +     ++E M+PYVSK+PR A+LNYRDVD+G +
Sbjct: 430 HRAGNMFKIQYAANWFVPGEAVAKDCLSQTERLFEAMSPYVSKNPREAFLNYRDVDIGKS 489

Query: 510 GNG---HASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEA 547
            N        +G KYFK NF++LVK+KS VDPDNFFRYEQSIP + +
Sbjct: 490 LNSTYEEGKVYGFKYFKDNFEKLVKIKSRVDPDNFFRYEQSIPVLSS 530

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_038883923.1 (berberine bridge enzyme-like 14 [Benincasa hispida])

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MEMPGCFFCFRHLPPNAISMGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDH 60
           MEMPGCFFCFRHLPPNAISMGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDH
Sbjct: 1   MEMPGCFFCFRHLPPNAISMGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDH 60

Query: 61  SAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCC 120
           SAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCC
Sbjct: 61  SAPSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCC 120

Query: 121 RIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYF 180
           RIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYF
Sbjct: 121 RIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYF 180

Query: 181 KIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRA 240
           KIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRA
Sbjct: 181 KIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRA 240

Query: 241 SMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIAD 300
           SMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIAD
Sbjct: 241 SMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIAD 300

Query: 301 KIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMS 360
           KIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMS
Sbjct: 301 KIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMS 360

Query: 361 WIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRP 420
           WIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRP
Sbjct: 361 WIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRP 420

Query: 421 ALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMT 480
           ALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMT
Sbjct: 421 ALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMT 480

Query: 481 PYVSKSPRSAYLNYRDVDLGVNGNGHASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQS 540
           PYVSKSPRSAYLNYRDVDLGVNGNGHASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQS
Sbjct: 481 PYVSKSPRSAYLNYRDVDLGVNGNGHASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQS 540

Query: 541 IPCVEAFDSNGRAESL 557
           IPCVEAFDSNGRAESL
Sbjct: 541 IPCVEAFDSNGRAESL 556

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_008439526.1 (PREDICTED: reticuline oxidase-like protein [Cucumis melo])

HSP 1 Score: 1006.5 bits (2601), Expect = 8.8e-290
Identity = 508/566 (89.75%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MEMPGCFFCFRHLPPNAISMG--IPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLS 60
           M++P CFF F HLPP AISMG  IP+S+LIP LTT LLIL SMA+S Q PSHQTLL CLS
Sbjct: 1   MQIPCCFFSFHHLPPKAISMGIPIPDSVLIPTLTTLLLILSSMALSAQTPSHQTLLDCLS 60

Query: 61  DHS-APSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI 120
           DHS +PSSSPIS+V FFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI
Sbjct: 61  DHSNSPSSSPISQVTFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI 120

Query: 121 LCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGE 180
           LCCRIHALEIRIRSGGHDYDGLSYVSDS FVILDMFNLRSVAVDIEDESAWVDSGATLGE
Sbjct: 121 LCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGE 180

Query: 181 VYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL 240
           VY+KIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL
Sbjct: 181 VYYKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL 240

Query: 241 DRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQE 300
           DR +MGEDLFWAIRGGGGASFGVIVSWKFKLV LPETVTVFRTEKT+EEGVVDIL+KWQE
Sbjct: 241 DRETMGEDLFWAIRGGGGASFGVIVSWKFKLVLLPETVTVFRTEKTIEEGVVDILHKWQE 300

Query: 301 IADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCI 360
           IAD IDENLFIRVVILPVNKK  KTAKAKF+SLFLGNAQKLFALMS++ PELGIK EDC 
Sbjct: 301 IADTIDENLFIRVVILPVNKKTQKTAKAKFVSLFLGNAQKLFALMSERFPELGIKGEDCK 360

Query: 361 EMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIEL 420
           EMSWIDS+LFWSNYPIGTPL+VLLERQP SEKFLKKKSDYVQEPISKA LEGMMRKMIEL
Sbjct: 361 EMSWIDSILFWSNYPIGTPLDVLLERQPNSEKFLKKKSDYVQEPISKAGLEGMMRKMIEL 420

Query: 421 KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYE 480
           KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAA K++E+IRE+Y+
Sbjct: 421 KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKHLEMIRELYK 480

Query: 481 YMTPYVSKSPRSAYLNYRDVDLGVNGNGHA-----SSWGRKYFKGNFDRLVKVKSMVDPD 540
           YMTPYVSKSPRSAYLNYRDVDLGVNG G+A     S WGRKYFKGNFDRLVKVKSMVDPD
Sbjct: 481 YMTPYVSKSPRSAYLNYRDVDLGVNGIGNASYWEGSIWGRKYFKGNFDRLVKVKSMVDPD 540

Query: 541 NFFRYEQSIPCV--EAFDSNGRAESL 557
           NFF+YEQSIPCV  EAFDSNGRAESL
Sbjct: 541 NFFKYEQSIPCVELEAFDSNGRAESL 566

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_004134895.1 (berberine bridge enzyme-like 21 [Cucumis sativus] >KAE8647769.1 hypothetical protein Csa_002773 [Cucumis sativus])

HSP 1 Score: 968.8 bits (2503), Expect = 2.0e-278
Identity = 485/536 (90.49%), Postives = 504/536 (94.03%), Query Frame = 0

Query: 28  IPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPSYSPVLNSY 87
           IP+LTT LLIL SMA+S Q PSHQTLL CL DHS+PSSSPISEV FFPDNPSYSPVLNSY
Sbjct: 3   IPILTTLLLILSSMALSAQFPSHQTLLHCLFDHSSPSSSPISEVTFFPDNPSYSPVLNSY 62

Query: 88  IRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTF 147
           IRNLRF SPTTPKPLFIV PTHVSH+QAT+LCCRIHALEIRIRSGGHDYDGLSYVS S F
Sbjct: 63  IRNLRFTSPTTPKPLFIVTPTHVSHVQATVLCCRIHALEIRIRSGGHDYDGLSYVSASPF 122

Query: 148 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSG 207
           VILDMFNLRSVAVDIEDESAWVDSGATLGEVY+KIAEKSKIHGFPAGVCPTVGVGGHLSG
Sbjct: 123 VILDMFNLRSVAVDIEDESAWVDSGATLGEVYYKIAEKSKIHGFPAGVCPTVGVGGHLSG 182

Query: 208 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFK 267
           AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDR +MGEDLFWAIRGGGGASFGVIVSWKFK
Sbjct: 183 AGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRETMGEDLFWAIRGGGGASFGVIVSWKFK 242

Query: 268 LVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKF 327
           LV LPETVTVFRTEKT+EEGVVDIL+KWQEIAD IDENLFIRVVILPVNKK  KTAKAKF
Sbjct: 243 LVLLPETVTVFRTEKTIEEGVVDILHKWQEIADTIDENLFIRVVILPVNKKTQKTAKAKF 302

Query: 328 ISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQPKS 387
           +SLFLGNAQKLFALMS++ PELGIK EDC EMSWI+S+LFWSNYPIGTPLNVLLERQP S
Sbjct: 303 VSLFLGNAQKLFALMSERFPELGIKDEDCKEMSWIESILFWSNYPIGTPLNVLLERQPNS 362

Query: 388 EKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGN 447
           EKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGN
Sbjct: 363 EKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGN 422

Query: 448 KYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGHA 507
           KYKIQYSVTWKEEGDEAA KN++ IRE+Y+YMTPYVSKSPRSAYLNYRDVDLGVNG G+A
Sbjct: 423 KYKIQYSVTWKEEGDEAAAKNLKKIRELYKYMTPYVSKSPRSAYLNYRDVDLGVNGIGNA 482

Query: 508 -----SSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPC--VEAFDSNGRAESL 557
                S WGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPC  VEA DSNGRAESL
Sbjct: 483 SYWVGSIWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEVEALDSNGRAESL 538

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_022925684.1 (berberine bridge enzyme-like 8 isoform X1 [Cucurbita moschata])

HSP 1 Score: 967.6 bits (2500), Expect = 4.5e-278
Identity = 480/542 (88.56%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79
           M IP SLLIP+LTT LLILPSMA +    SH TLL CLSDHS+PS+SPISEV FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139
           YSPVLNSYIRNLRFASPTTPKPLFIVAPTH+SHIQA+ILCCRIHALE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199
           SYVSDS FVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259
           GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVD NGR LDR SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRALDRESMGEDLFWAIRGGGGASFG 240

Query: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319
           VIVSWKF+LVPLPETV+VFR EKTMEEG VD+LYKWQE+ADKIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFRLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379
            KTAKAKFISLFLG+AQKLF+LMS+ LPELG+K EDC+EMSWI+SVLFWSNYPIGTPLNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439
           LLERQP SEKFLKKKSDYVQEPISKADLEGM+RKM+ELKRPALTFNPYGGKMS+I E ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAERET 420

Query: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499
           PFPHRAGNKYKIQYSVTWKEEG+EAADKNVELIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 500 GVNGNGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFDSNGRAE 557
           GVNGNG+     AS WGRKYFKGN DRLV+VK+MVDP NFFRYEQSIP   A+DSNGRAE
Sbjct: 481 GVNGNGNASYWEASHWGRKYFKGNLDRLVEVKTMVDPGNFFRYEQSIPSAAAYDSNGRAE 538

BLAST of Bhi03G000165 vs. NCBI nr
Match: XP_022977474.1 (berberine bridge enzyme-like 8 isoform X1 [Cucurbita maxima])

HSP 1 Score: 965.3 bits (2494), Expect = 2.3e-277
Identity = 478/542 (88.19%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79
           M IP SLLIP+LTT LLILPSMA +    SH TLL CLSDHS+PS+SPISEV FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139
           YSPVLNSYIRNLRFASPTTPKPLFIVAPTH+SHIQA+ILCCRIHALE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199
           SYVSDS FVILDMFNLRSV VDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVDVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259
           GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVD NGRVLDR SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASFG 240

Query: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319
           VIVSWKFKLVPLPETV+VFR EKTMEEG VD+LYKWQE+ADKIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFKLVPLPETVSVFRIEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379
            KTAKAKFISLFLG+AQKL++L+S+ LPELG+K EDC+EMSWI+SVLFWSNYPIGTPLNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLYSLISETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439
           LLERQP SEKFLKKKSDYVQEPISKADLEGM+RKM+ELKRPALTFNPYGGKMS+I E+ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAESET 420

Query: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499
           PFPHRAGNKYKIQYSVTWKEEG+EAADKNVELIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 500 GVNGNGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFDSNGRAE 557
           GVNGNG+     AS WGRKYFKGNFDRLV+VK+MVDP NFFRYEQSIP   A+D NGR E
Sbjct: 481 GVNGNGNASYWEASQWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAYDCNGRGE 538

BLAST of Bhi03G000165 vs. ExPASy TrEMBL
Match: A0A1S3AZM6 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103484295 PE=3 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.3e-290
Identity = 508/566 (89.75%), Postives = 531/566 (93.82%), Query Frame = 0

Query: 1   MEMPGCFFCFRHLPPNAISMG--IPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLS 60
           M++P CFF F HLPP AISMG  IP+S+LIP LTT LLIL SMA+S Q PSHQTLL CLS
Sbjct: 1   MQIPCCFFSFHHLPPKAISMGIPIPDSVLIPTLTTLLLILSSMALSAQTPSHQTLLDCLS 60

Query: 61  DHS-APSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI 120
           DHS +PSSSPIS+V FFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI
Sbjct: 61  DHSNSPSSSPISQVTFFPDNPSYSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATI 120

Query: 121 LCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGE 180
           LCCRIHALEIRIRSGGHDYDGLSYVSDS FVILDMFNLRSVAVDIEDESAWVDSGATLGE
Sbjct: 121 LCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGE 180

Query: 181 VYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL 240
           VY+KIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL
Sbjct: 181 VYYKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVL 240

Query: 241 DRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQE 300
           DR +MGEDLFWAIRGGGGASFGVIVSWKFKLV LPETVTVFRTEKT+EEGVVDIL+KWQE
Sbjct: 241 DRETMGEDLFWAIRGGGGASFGVIVSWKFKLVLLPETVTVFRTEKTIEEGVVDILHKWQE 300

Query: 301 IADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCI 360
           IAD IDENLFIRVVILPVNKK  KTAKAKF+SLFLGNAQKLFALMS++ PELGIK EDC 
Sbjct: 301 IADTIDENLFIRVVILPVNKKTQKTAKAKFVSLFLGNAQKLFALMSERFPELGIKGEDCK 360

Query: 361 EMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIEL 420
           EMSWIDS+LFWSNYPIGTPL+VLLERQP SEKFLKKKSDYVQEPISKA LEGMMRKMIEL
Sbjct: 361 EMSWIDSILFWSNYPIGTPLDVLLERQPNSEKFLKKKSDYVQEPISKAGLEGMMRKMIEL 420

Query: 421 KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYE 480
           KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAA K++E+IRE+Y+
Sbjct: 421 KRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKEEGDEAAAKHLEMIRELYK 480

Query: 481 YMTPYVSKSPRSAYLNYRDVDLGVNGNGHA-----SSWGRKYFKGNFDRLVKVKSMVDPD 540
           YMTPYVSKSPRSAYLNYRDVDLGVNG G+A     S WGRKYFKGNFDRLVKVKSMVDPD
Sbjct: 481 YMTPYVSKSPRSAYLNYRDVDLGVNGIGNASYWEGSIWGRKYFKGNFDRLVKVKSMVDPD 540

Query: 541 NFFRYEQSIPCV--EAFDSNGRAESL 557
           NFF+YEQSIPCV  EAFDSNGRAESL
Sbjct: 541 NFFKYEQSIPCVELEAFDSNGRAESL 566

BLAST of Bhi03G000165 vs. ExPASy TrEMBL
Match: A0A6J1ECX0 (berberine bridge enzyme-like 8 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433031 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.2e-278
Identity = 480/542 (88.56%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79
           M IP SLLIP+LTT LLILPSMA +    SH TLL CLSDHS+PS+SPISEV FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139
           YSPVLNSYIRNLRFASPTTPKPLFIVAPTH+SHIQA+ILCCRIHALE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199
           SYVSDS FVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259
           GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVD NGR LDR SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRALDRESMGEDLFWAIRGGGGASFG 240

Query: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319
           VIVSWKF+LVPLPETV+VFR EKTMEEG VD+LYKWQE+ADKIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFRLVPLPETVSVFRMEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379
            KTAKAKFISLFLG+AQKLF+LMS+ LPELG+K EDC+EMSWI+SVLFWSNYPIGTPLNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLFSLMSETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439
           LLERQP SEKFLKKKSDYVQEPISKADLEGM+RKM+ELKRPALTFNPYGGKMS+I E ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAERET 420

Query: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499
           PFPHRAGNKYKIQYSVTWKEEG+EAADKNVELIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 500 GVNGNGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFDSNGRAE 557
           GVNGNG+     AS WGRKYFKGN DRLV+VK+MVDP NFFRYEQSIP   A+DSNGRAE
Sbjct: 481 GVNGNGNASYWEASHWGRKYFKGNLDRLVEVKTMVDPGNFFRYEQSIPSAAAYDSNGRAE 538

BLAST of Bhi03G000165 vs. ExPASy TrEMBL
Match: A0A6J1IRG6 (berberine bridge enzyme-like 8 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477807 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 478/542 (88.19%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79
           M IP SLLIP+LTT LLILPSMA +    SH TLL CLSDHS+PS+SPISEV FFPDNPS
Sbjct: 1   MAIPTSLLIPILTTLLLILPSMAAA----SHHTLLSCLSDHSSPSASPISEVTFFPDNPS 60

Query: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139
           YSPVLNSYIRNLRFASPTTPKPLFIVAPTH+SHIQA+ILCCRIHALE+RIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHISHIQASILCCRIHALELRIRSGGHDYDGL 120

Query: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199
           SYVSDS FVILDMFNLRSV VDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV
Sbjct: 121 SYVSDSPFVILDMFNLRSVDVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 180

Query: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259
           GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVD NGRVLDR SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDSNGRVLDRESMGEDLFWAIRGGGGASFG 240

Query: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319
           VIVSWKFKLVPLPETV+VFR EKTMEEG VD+LYKWQE+ADKIDENLFIRVVILPVNKK 
Sbjct: 241 VIVSWKFKLVPLPETVSVFRIEKTMEEGAVDMLYKWQEVADKIDENLFIRVVILPVNKKT 300

Query: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379
            KTAKAKFISLFLG+AQKL++L+S+ LPELG+K EDC+EMSWI+SVLFWSNYPIGTPLNV
Sbjct: 301 QKTAKAKFISLFLGSAQKLYSLISETLPELGVKAEDCLEMSWIESVLFWSNYPIGTPLNV 360

Query: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439
           LLERQP SEKFLKKKSDYVQEPISKADLEGM+RKM+ELKRPALTFNPYGGKMS+I E+ET
Sbjct: 361 LLERQPNSEKFLKKKSDYVQEPISKADLEGMLRKMMELKRPALTFNPYGGKMSEIAESET 420

Query: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499
           PFPHRAGNKYKIQYSVTWKEEG+EAADKNVELIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNKYKIQYSVTWKEEGEEAADKNVELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 500 GVNGNGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAFDSNGRAE 557
           GVNGNG+     AS WGRKYFKGNFDRLV+VK+MVDP NFFRYEQSIP   A+D NGR E
Sbjct: 481 GVNGNGNASYWEASQWGRKYFKGNFDRLVEVKTMVDPGNFFRYEQSIPSAAAYDCNGRGE 538

BLAST of Bhi03G000165 vs. ExPASy TrEMBL
Match: A0A5D3CQQ4 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009800 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.5e-274
Identity = 478/524 (91.22%), Postives = 499/524 (95.23%), Query Frame = 0

Query: 41  MAVSDQIPSHQTLLKCLSDHS-APSSSPISEVIFFPDNPSYSPVLNSYIRNLRFASPTTP 100
           MA+S Q PSHQTLL CLSDHS +PSSSPIS+V FFPDNPSYSPVLNSYIRNLRFASPTTP
Sbjct: 1   MALSAQTPSHQTLLDCLSDHSNSPSSSPISQVTFFPDNPSYSPVLNSYIRNLRFASPTTP 60

Query: 101 KPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSTFVILDMFNLRSVA 160
           KPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDS FVILDMFNLRSVA
Sbjct: 61  KPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGLSYVSDSPFVILDMFNLRSVA 120

Query: 161 VDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGV 220
           VDI+DESAWVDSGATLGEVY+KIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGV
Sbjct: 121 VDIKDESAWVDSGATLGEVYYKIAEKSKIHGFPAGVCPTVGVGGHLSGAGYGNLMRKFGV 180

Query: 221 SVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFGVIVSWKFKLVPLPETVTVFR 280
           SVDNVVDALIVDVNGRVLDR +MGEDLFWAIRGGGGASFGVIVSWKFKLV LPETVTVFR
Sbjct: 181 SVDNVVDALIVDVNGRVLDRETMGEDLFWAIRGGGGASFGVIVSWKFKLVLLPETVTVFR 240

Query: 281 TEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKALKTAKAKFISLFLGNAQKLF 340
           TEKT+EEGVVDIL+KWQEIAD IDENLFIRVVILPVNKK  KTAKAKF+SLFLGNAQKLF
Sbjct: 241 TEKTIEEGVVDILHKWQEIADTIDENLFIRVVILPVNKKTQKTAKAKFVSLFLGNAQKLF 300

Query: 341 ALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNVLLERQPKSEKFLKKKSDYVQ 400
           ALMS++ PELGIK EDC EMSWIDS+LFWSNYPIGTPL+VLLERQP SEKFLKKKSDYVQ
Sbjct: 301 ALMSERFPELGIKGEDCKEMSWIDSILFWSNYPIGTPLDVLLERQPNSEKFLKKKSDYVQ 360

Query: 401 EPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKE 460
           EPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKE
Sbjct: 361 EPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETETPFPHRAGNKYKIQYSVTWKE 420

Query: 461 EGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDLGVNGNGHA-----SSWGRKY 520
           EGDEAA K++E+IRE+Y+YMTPYVSKSPRSAYLNYRDVDLGVNG G+A     S WGRKY
Sbjct: 421 EGDEAAAKHLEMIRELYKYMTPYVSKSPRSAYLNYRDVDLGVNGIGNASYWEGSIWGRKY 480

Query: 521 FKGNFDRLVKVKSMVDPDNFFRYEQSIPCV--EAFDSNGRAESL 557
           FKGNFDRLVKVKSMVDPDNFF+YEQSIPCV  EAFDSNGRA+SL
Sbjct: 481 FKGNFDRLVKVKSMVDPDNFFKYEQSIPCVELEAFDSNGRAKSL 524

BLAST of Bhi03G000165 vs. ExPASy TrEMBL
Match: A0A6J1D0D6 (berberine bridge enzyme-like 21 OS=Momordica charantia OX=3673 GN=LOC111015859 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 7.3e-266
Identity = 464/548 (84.67%), Postives = 500/548 (91.24%), Query Frame = 0

Query: 20  MGIPNSLLIPVLTTFLLILPSMAVSDQIPSHQTLLKCLSDHSAPSSSPISEVIFFPDNPS 79
           M IPNS +IP+   FL +L SMA S  IPS Q LL+CLSDHS+PSSSPIS+++FFPDNPS
Sbjct: 1   MAIPNSPIIPI---FLTLLASMAASHPIPSSQILLQCLSDHSSPSSSPISQLVFFPDNPS 60

Query: 80  YSPVLNSYIRNLRFASPTTPKPLFIVAPTHVSHIQATILCCRIHALEIRIRSGGHDYDGL 139
           YSPVLNSYIRNLRFASPTTP PLFIVAPTHVSHIQA+I+CCRIH LEIRIRSGGHDYDGL
Sbjct: 61  YSPVLNSYIRNLRFASPTTPNPLFIVAPTHVSHIQASIICCRIHGLEIRIRSGGHDYDGL 120

Query: 140 SYVSDSTFVILDMFNLRSVAVDIEDESAWVDSGATLGEVYFKIAEKSKIHGFPAGVCPTV 199
           SYVSDS FVI+DMFNLRSVAVDIEDESAWV+SGATLGEVYFKIAE+SKI+GFPAGVCPTV
Sbjct: 121 SYVSDSPFVIVDMFNLRSVAVDIEDESAWVESGATLGEVYFKIAERSKIYGFPAGVCPTV 180

Query: 200 GVGGHLSGAGYGNLMRKFGVSVDNVVDALIVDVNGRVLDRASMGEDLFWAIRGGGGASFG 259
           GVGGHLSGAGYGNLMRKFGVSVD VVDALIVD NGRVLDR SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHLSGAGYGNLMRKFGVSVDYVVDALIVDANGRVLDRESMGEDLFWAIRGGGGASFG 240

Query: 260 VIVSWKFKLVPLPETVTVFRTEKTMEEGVVDILYKWQEIADKIDENLFIRVVILPVNKKA 319
           +IVSWKFKLVPLPETVTVFR EKTMEEG VDILYKWQ+IA KIDENLFIRVVILPVNKKA
Sbjct: 241 IIVSWKFKLVPLPETVTVFRMEKTMEEGAVDILYKWQQIAHKIDENLFIRVVILPVNKKA 300

Query: 320 LKTAKAKFISLFLGNAQKLFALMSKKLPELGIKVEDCIEMSWIDSVLFWSNYPIGTPLNV 379
             TAKAKF+SLFLGNA+KLFALMS+  PELG+K EDC EMSWI+SVLFWSNYP GT LNV
Sbjct: 301 KSTAKAKFVSLFLGNAEKLFALMSESFPELGVKAEDCKEMSWIESVLFWSNYPNGTSLNV 360

Query: 380 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMRKMIELKRPALTFNPYGGKMSQIPETET 439
           LLERQPKSEKFLKKKSDYVQEPISKADLEGMM+KM+ELKRPALTFNPYGGKMSQIPE+ET
Sbjct: 361 LLERQPKSEKFLKKKSDYVQEPISKADLEGMMKKMMELKRPALTFNPYGGKMSQIPESET 420

Query: 440 PFPHRAGNKYKIQYSVTWKEEGDEAADKNVELIREMYEYMTPYVSKSPRSAYLNYRDVDL 499
           PFPHRAGN YKIQYSVTWKEEGDEAA++N+ELIRE+YEYMTP+VSKSPRSAYLNYRDVDL
Sbjct: 421 PFPHRAGNMYKIQYSVTWKEEGDEAAEQNLELIRELYEYMTPHVSKSPRSAYLNYRDVDL 480

Query: 500 GVNG-NGH-----ASSWGRKYFKGNFDRLVKVKSMVDPDNFFRYEQSIPCVEAF-----D 557
           GVNG NG+     AS+WGRKYFKGN +RLV+VK++ DPDNFFRYEQSIP V  F     D
Sbjct: 481 GVNGRNGNGSYEEASTWGRKYFKGNLERLVEVKTVADPDNFFRYEQSIPSVAFFKNQQSD 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G20840.15.7e-16254.13FAD-binding Berberine family protein [more]
AT1G34575.11.4e-16053.93FAD-binding Berberine family protein [more]
AT1G30700.11.8e-16053.44FAD-binding Berberine family protein [more]
AT4G20830.22.0e-15952.84FAD-binding Berberine family protein [more]
AT4G20830.12.7e-15952.94FAD-binding Berberine family protein [more]
Match NameE-valueIdentityDescription
Q9SVG38.1e-16154.13Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=... [more]
F4HV092.0e-15953.93Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=... [more]
Q9SA852.6e-15953.44Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2... [more]
Q9SVG43.7e-15852.94Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=... [more]
Q9SA861.3e-15552.56Berberine bridge enzyme-like 9 OS=Arabidopsis thaliana OX=3702 GN=At1g30710 PE=2... [more]
Match NameE-valueIdentityDescription
XP_038883923.10.0e+00100.00berberine bridge enzyme-like 14 [Benincasa hispida][more]
XP_008439526.18.8e-29089.75PREDICTED: reticuline oxidase-like protein [Cucumis melo][more]
XP_004134895.12.0e-27890.49berberine bridge enzyme-like 21 [Cucumis sativus] >KAE8647769.1 hypothetical pro... [more]
XP_022925684.14.5e-27888.56berberine bridge enzyme-like 8 isoform X1 [Cucurbita moschata][more]
XP_022977474.12.3e-27788.19berberine bridge enzyme-like 8 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A1S3AZM64.3e-29089.75reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103484295 PE=3 SV=... [more]
A0A6J1ECX02.2e-27888.56berberine bridge enzyme-like 8 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IRG61.1e-27788.19berberine bridge enzyme-like 8 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A5D3CQQ41.5e-27491.22Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1D0D67.3e-26684.67berberine bridge enzyme-like 21 OS=Momordica charantia OX=3673 GN=LOC111015859 P... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 101..237
e-value: 3.5E-23
score: 81.9
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 160..542
e-value: 1.3E-161
score: 540.2
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 48..155
e-value: 5.2E-41
score: 140.8
NoneNo IPR availableGENE3D3.40.462.20coord: 274..486
e-value: 1.3E-161
score: 540.2
NoneNo IPR availablePANTHERPTHR32448:SF12RETICULINE OXIDASE-LIKE PROTEINcoord: 29..547
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 29..547
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 490..542
e-value: 3.1E-22
score: 78.4
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 97..271
score: 17.250959
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 73..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000165Bhi03M000165mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding