Bhi03G000162 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000162
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionFAD-binding Berberine family protein
Locationchr3: 4273241 .. 4274896 (-)
RNA-Seq ExpressionBhi03G000162
SyntenyBhi03G000162
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGTTTACAAATCCCAAGTACAAATCCTTTGTGGCTTCTCTGTTTCTTGTCTTTCTGCTTTCATCTTCTTCTTGGGTTGATTCAGCTTCTCTTGAGGAGAGTTTCCTTCAGTGTCTAAATGAGAATTCTCAATTTTCTGTCCCTTACTCATCTTTTTGTGCTCCAAACAATGCTACATTTAATGCTCTTTTGCAATCAACTGCTCAGAATCTGAGGTACTTGGAGCCTTCAGTGCCAAAGCCTCTGTTCATCTTCACCCCATTGTTTGATTCCCATGTCCAGTCAGCTGTTATTTGCTCAAAACAGCTCAAGATTCATCTCAGAGTTCGTAGTGGAGGCCATGACTATGAGGGCCTCTCTTATGTGTCTGAAATTGAGACACCTTTCATTATTCTTGACCTTGCCAAGCTTCGATCTGTCAAAGTGGACATTGATGGTAATAGCGCTTGGGTTCAGGCTGGGGCGACGATTGGCGAGGTTTATTACAGAATTGCTGAGAAAAGTAAGGTTCATGGCTTCCCAGCTGGTCTTTGCACAAGTTTGGGTGTTGGAGGTCATATAACAGGTGGTGCTTATGGTTCTATGATGCGAAAATACGGTCTTGGTGCTGACAATGTTGTAGATGCTCGGATTGTTGATGCAAATGGGAGAATTCTTAACAGAGAAGCCATGGGAGAGGACCTGTTTTGGGCTATTAGAGGAGGAGGAGGGGGGAGCTTTGGAATCATTCTGTGGTGGAAGCTAAATTTGGTTCCTGTTCCATCAACTGTGACTGTTTTTACAGTTGCAAAGACTTTAGAACAAGGTGCTACAAAAATCCTGTACAAATGGCAACAGGTTGCTGATAAGCTAGATGAAGATTTGTTCATCAGAGTGATTATAAATGTGGCTGCAAATGCTGATAAAAAGGGTCAGAAAACTGTAAGTACAGCTTACAATGCCCTCTTCTTGGGAGATGCAAGCAGACTCCTCAAAGTTATGGGAGAAAGCTTTCCTGAATTGGGTTTGACAAGAAAAGATTGCATAGAGACAAGCTGGATCAAATCAGTTCTTTACATTGCTGGTTACCCAAGTGGAACTCCACCAGAAGTGCTTCTCCAAGGCAAATCAACTTTCAAAAACTACTTCAAAGCAAAATCAGACTTTGTCAAAGACCCCATTCCAGAAACAGGTCTTGAAGGGCTATGGAAAAGGCTGTTTGAAGATGAAAGTCCATTGATGATATGGAATCCTTATGGTGGAATGATGGGCAAGATTTCGGAGACTGAGATTCCATTTCCTCATAGAAGAGGAGTCCTTTTCAAAATTCAGTACCTAACTACATGGCAAAAAGGAGAGGAAAATCAAGACAAACACCTACAATGGATCAAGAAGCTTTACAATTACATGACACCTTATGTTTCTCAGCTTCCAAGAGAAACATATGTGAATTACAGAGATCTTGACTTGGGAATTAACAAGAACAGCAACACAAGCTACATTGAATCAATCGGATGGGGAACTCGGTATTTCAAAGAAAACTTTGGGAGATTGCTGAGAGTTAAGACTAAAGTCGATCCTGATAACTTCTTCCGACATGAACAGAGCATTCCACCTATTCCAACTTCTGAAAGTTTAAGAAGCTTTGGCTCTTCCAAAAGGGGATTTGATTAG

mRNA sequence

ATGGTGTTTACAAATCCCAAGTACAAATCCTTTGTGGCTTCTCTGTTTCTTGTCTTTCTGCTTTCATCTTCTTCTTGGGTTGATTCAGCTTCTCTTGAGGAGAGTTTCCTTCAGTGTCTAAATGAGAATTCTCAATTTTCTGTCCCTTACTCATCTTTTTGTGCTCCAAACAATGCTACATTTAATGCTCTTTTGCAATCAACTGCTCAGAATCTGAGGTACTTGGAGCCTTCAGTGCCAAAGCCTCTGTTCATCTTCACCCCATTGTTTGATTCCCATGTCCAGTCAGCTGTTATTTGCTCAAAACAGCTCAAGATTCATCTCAGAGTTCGTAGTGGAGGCCATGACTATGAGGGCCTCTCTTATGTGTCTGAAATTGAGACACCTTTCATTATTCTTGACCTTGCCAAGCTTCGATCTGTCAAAGTGGACATTGATGGTAATAGCGCTTGGGTTCAGGCTGGGGCGACGATTGGCGAGGTTTATTACAGAATTGCTGAGAAAAGTAAGGTTCATGGCTTCCCAGCTGGTCTTTGCACAAGTTTGGGTGTTGGAGGTCATATAACAGGTGGTGCTTATGGTTCTATGATGCGAAAATACGGTCTTGGTGCTGACAATGTTGTAGATGCTCGGATTGTTGATGCAAATGGGAGAATTCTTAACAGAGAAGCCATGGGAGAGGACCTGTTTTGGGCTATTAGAGGAGGAGGAGGGGGGAGCTTTGGAATCATTCTGTGGTGGAAGCTAAATTTGGTTCCTGTTCCATCAACTGTGACTGTTTTTACAGTTGCAAAGACTTTAGAACAAGGTGCTACAAAAATCCTGTACAAATGGCAACAGGTTGCTGATAAGCTAGATGAAGATTTGTTCATCAGAGTGATTATAAATGTGGCTGCAAATGCTGATAAAAAGGGTCAGAAAACTGTAAGTACAGCTTACAATGCCCTCTTCTTGGGAGATGCAAGCAGACTCCTCAAAGTTATGGGAGAAAGCTTTCCTGAATTGGGTTTGACAAGAAAAGATTGCATAGAGACAAGCTGGATCAAATCAGTTCTTTACATTGCTGGTTACCCAAGTGGAACTCCACCAGAAGTGCTTCTCCAAGGCAAATCAACTTTCAAAAACTACTTCAAAGCAAAATCAGACTTTGTCAAAGACCCCATTCCAGAAACAGGTCTTGAAGGGCTATGGAAAAGGCTGTTTGAAGATGAAAGTCCATTGATGATATGGAATCCTTATGGTGGAATGATGGGCAAGATTTCGGAGACTGAGATTCCATTTCCTCATAGAAGAGGAGTCCTTTTCAAAATTCAGTACCTAACTACATGGCAAAAAGGAGAGGAAAATCAAGACAAACACCTACAATGGATCAAGAAGCTTTACAATTACATGACACCTTATGTTTCTCAGCTTCCAAGAGAAACATATGTGAATTACAGAGATCTTGACTTGGGAATTAACAAGAACAGCAACACAAGCTACATTGAATCAATCGGATGGGGAACTCGGTATTTCAAAGAAAACTTTGGGAGATTGCTGAGAGTTAAGACTAAAGTCGATCCTGATAACTTCTTCCGACATGAACAGAGCATTCCACCTATTCCAACTTCTGAAAGTTTAAGAAGCTTTGGCTCTTCCAAAAGGGGATTTGATTAG

Coding sequence (CDS)

ATGGTGTTTACAAATCCCAAGTACAAATCCTTTGTGGCTTCTCTGTTTCTTGTCTTTCTGCTTTCATCTTCTTCTTGGGTTGATTCAGCTTCTCTTGAGGAGAGTTTCCTTCAGTGTCTAAATGAGAATTCTCAATTTTCTGTCCCTTACTCATCTTTTTGTGCTCCAAACAATGCTACATTTAATGCTCTTTTGCAATCAACTGCTCAGAATCTGAGGTACTTGGAGCCTTCAGTGCCAAAGCCTCTGTTCATCTTCACCCCATTGTTTGATTCCCATGTCCAGTCAGCTGTTATTTGCTCAAAACAGCTCAAGATTCATCTCAGAGTTCGTAGTGGAGGCCATGACTATGAGGGCCTCTCTTATGTGTCTGAAATTGAGACACCTTTCATTATTCTTGACCTTGCCAAGCTTCGATCTGTCAAAGTGGACATTGATGGTAATAGCGCTTGGGTTCAGGCTGGGGCGACGATTGGCGAGGTTTATTACAGAATTGCTGAGAAAAGTAAGGTTCATGGCTTCCCAGCTGGTCTTTGCACAAGTTTGGGTGTTGGAGGTCATATAACAGGTGGTGCTTATGGTTCTATGATGCGAAAATACGGTCTTGGTGCTGACAATGTTGTAGATGCTCGGATTGTTGATGCAAATGGGAGAATTCTTAACAGAGAAGCCATGGGAGAGGACCTGTTTTGGGCTATTAGAGGAGGAGGAGGGGGGAGCTTTGGAATCATTCTGTGGTGGAAGCTAAATTTGGTTCCTGTTCCATCAACTGTGACTGTTTTTACAGTTGCAAAGACTTTAGAACAAGGTGCTACAAAAATCCTGTACAAATGGCAACAGGTTGCTGATAAGCTAGATGAAGATTTGTTCATCAGAGTGATTATAAATGTGGCTGCAAATGCTGATAAAAAGGGTCAGAAAACTGTAAGTACAGCTTACAATGCCCTCTTCTTGGGAGATGCAAGCAGACTCCTCAAAGTTATGGGAGAAAGCTTTCCTGAATTGGGTTTGACAAGAAAAGATTGCATAGAGACAAGCTGGATCAAATCAGTTCTTTACATTGCTGGTTACCCAAGTGGAACTCCACCAGAAGTGCTTCTCCAAGGCAAATCAACTTTCAAAAACTACTTCAAAGCAAAATCAGACTTTGTCAAAGACCCCATTCCAGAAACAGGTCTTGAAGGGCTATGGAAAAGGCTGTTTGAAGATGAAAGTCCATTGATGATATGGAATCCTTATGGTGGAATGATGGGCAAGATTTCGGAGACTGAGATTCCATTTCCTCATAGAAGAGGAGTCCTTTTCAAAATTCAGTACCTAACTACATGGCAAAAAGGAGAGGAAAATCAAGACAAACACCTACAATGGATCAAGAAGCTTTACAATTACATGACACCTTATGTTTCTCAGCTTCCAAGAGAAACATATGTGAATTACAGAGATCTTGACTTGGGAATTAACAAGAACAGCAACACAAGCTACATTGAATCAATCGGATGGGGAACTCGGTATTTCAAAGAAAACTTTGGGAGATTGCTGAGAGTTAAGACTAAAGTCGATCCTGATAACTTCTTCCGACATGAACAGAGCATTCCACCTATTCCAACTTCTGAAAGTTTAAGAAGCTTTGGCTCTTCCAAAAGGGGATTTGATTAG

Protein sequence

MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNATFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESLRSFGSSKRGFD
Homology
BLAST of Bhi03G000162 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 740.3 bits (1910), Expect = 1.1e-213
Identity = 348/523 (66.54%), Postives = 439/523 (83.94%), Query Frame = 0

Query: 13  ASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPY-SSFCAPN-NAT-FNALLQSTA 72
           A+LFLV LL  S  + S++L++ F++CL +NS  S P  +SF +P+ NAT F   L+STA
Sbjct: 10  ATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTA 69

Query: 73  QNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEIETP 132
           QNLRYL PS PKP+FIF PL+++HVQ+AV+C+K+L++HLR+RSGGHDYEGLS+V+E ETP
Sbjct: 70  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 129

Query: 133 FIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHIT 192
           F+I+DL+KLR V VD+D NSAW  AGATIGEVYYRI EKS+ HGFPAGLC+SLG+GGH+ 
Sbjct: 130 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 189

Query: 193 GGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILWWKL 252
           GGAYGSMMRK+GLGADNV+DARIVDANG+IL+R AMGED+FWAIRGGGGGSFG+IL WK+
Sbjct: 190 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 249

Query: 253 NLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTV 312
            LVPVP+TVTVFTV KTLEQ  TK+LYKW+Q+ADKLD+DLFIRVII+ A+   K G +T+
Sbjct: 250 KLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTI 309

Query: 313 STAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQG 372
           S +Y A FLGD++RLL+VM +SFPELGLT+KDC E SWIKSV+YIAG+P+   PE LL G
Sbjct: 310 SMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAG 369

Query: 373 KSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPFPH 432
           KS FKN+FKAKSDFVK+PIP  GLEGLW+R  E++SPL IWNPYGGMM +ISE+EIPFPH
Sbjct: 370 KSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPH 429

Query: 433 RRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDLGINKN 492
           R G LFKIQ+L+TWQ G+ ++++H++WI+++Y+YM  YVS+ PR+ YVNYRDLDLG N+ 
Sbjct: 430 RNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEG 489

Query: 493 SNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIP 533
                 ++  WG +Y+K NF RL+++K + DPDNFFRHEQS+P
Sbjct: 490 ET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Bhi03G000162 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 726.5 bits (1874), Expect = 1.6e-209
Identity = 345/524 (65.84%), Postives = 425/524 (81.11%), Query Frame = 0

Query: 14  SLFLVFLLSSSSWV--DSASLEESFLQCLNENSQFSVPY-SSFCAP--NNATFNALLQST 73
           +  +  LL S S++    +++++ F+ CL +NS  S P  SSF     N ++F   L+++
Sbjct: 11  AFLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETS 70

Query: 74  AQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEIET 133
           AQNLRYL PS PKP FIF PL+++HVQ+AV+C+K+LK+HLR+RSGGHDYEGLSYVSE+ET
Sbjct: 71  AQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMET 130

Query: 134 PFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHI 193
            F+I+DL+KLR + VDI+ NSAWV AGA+IGEVYYRI EKSK+HGFPAGLCTSLG+GGHI
Sbjct: 131 AFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHI 190

Query: 194 TGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILWWK 253
            GGAYGSMMRK+GLGADNV+DARIVDA+G+ILNR AMGED+FWAIRGGGGGSFG+IL WK
Sbjct: 191 IGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWK 250

Query: 254 LNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKT 313
           + LVPVP  VTVFTV +TLEQ  TK+LYKWQQVADKLDEDLFIRVII   +   K  ++T
Sbjct: 251 IKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERT 310

Query: 314 VSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQ 373
           +ST+Y   FLGDA+RLL+VM  SFP+LGLT+KDC+ETSWIKSV+YIAG+PS  P E LL 
Sbjct: 311 ISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD 370

Query: 374 GKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPFP 433
           GKS FKNYFKAKSD+V++PIP  GLEGLW++L E++SPL IWNPYGGMM KI ETE PFP
Sbjct: 371 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFP 430

Query: 434 HRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDLGINK 493
           HR G LFKIQ+LT WQ G+ ++ KH+ W++++Y+YM  YVS+ PR  YVNYRDLDLG+N 
Sbjct: 431 HRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNG 490

Query: 494 NSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIP 533
             +    ++  WG RYFK NF RL+ +K K DP+NFFRHEQSIP
Sbjct: 491 KGS----DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Bhi03G000162 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 701.0 bits (1808), Expect = 7.3e-202
Identity = 338/531 (63.65%), Postives = 427/531 (80.41%), Query Frame = 0

Query: 10  SFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPY-SSFCAP--NNATFNALLQ 69
           S +   F ++ ++ +S    ASL++ F+ C+  N+    P   +F AP  N + F+ +L+
Sbjct: 11  SILVLYFSLYTITPTS--SLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLE 70

Query: 70  STAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEI 129
           STAQNLR+L+ S+PKP FIF+P+ +SHVQ+++ICSK+L++HLRVRSGGHDYEGLSYVS+I
Sbjct: 71  STAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQI 130

Query: 130 ETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 189
           + PFI++DL+K+R V ++I  NSAWVQ+GAT+GE+YYRIAEKSKVHGFPAGLC+SLG+GG
Sbjct: 131 DKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGG 190

Query: 190 HITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILW 249
           HITGGAYGSMMRKYGLGADNV+DA+IVDANG++L+R AMGED FWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILA 250

Query: 250 WKLNLVPVPSTVTVFTVAKTLEQG-ATKILYKWQQVADKLDEDLFIRVIINVAANADKKG 309
           WK+ LVPVP TVTVFTV KTL+Q    KI+ KWQ+VADKL E+LFIRV+ NVA      G
Sbjct: 251 WKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTG---G 310

Query: 310 QKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEV 369
            KTV+T+YNALFLG    L+ VM +SFPELGLT KDCIE SW++S+ YI+G+P+ TP  V
Sbjct: 311 NKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNV 370

Query: 370 LLQGKSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETE 429
           LLQGKS F K  FKAKSDFVK PIPE+GL+G++K+L +++ PLMIWNPYGGMM KI E++
Sbjct: 371 LLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQ 430

Query: 430 IPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDL 489
           IPFPHR+GVLFK+QY+T+W   ++   +H+ WI+ LY+YMTPYVS  PRE YVNYRDLDL
Sbjct: 431 IPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDL 490

Query: 490 GIN-KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPI 535
           G N K+  T   ++  WG  YFK NF RL+ +K KVDP+NFFRHEQSIPP+
Sbjct: 491 GRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Bhi03G000162 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 659.4 bits (1700), Expect = 2.4e-189
Identity = 321/534 (60.11%), Postives = 409/534 (76.59%), Query Frame = 0

Query: 10  SFVASLFLVFLLSSSSWVDSASLEESFLQCL--NENSQFSVPYSSFCAPNNAT-FNALLQ 69
           S ++   L F   + +   S SL++ F++CL  N N +F++  + F    NA+ F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 70  STAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEI 129
           STAQN RYL  ++PKP FIF P+ +SHVQ++VICSK+L+IH RVRSGGHDYEG+SYVS+I
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 130 ETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 189
           E PF+++DL+KLR + VDI   SAWV+AGAT+GE+YYRIAEKSK HGFPAG+  SLG+GG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 190 HITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILW 249
           HITGGAYGS+MRKYGL ADNV+DA+IVDANG++L+R +MGEDLFWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 250 WKLNLVPVPSTVTVFTVAKTLEQGAT-KILYKWQQVADKLDEDLFIRVIINVAANADKKG 309
           WK+ LVPVP T+TVFTV KT EQ  + KIL KWQ++AD L ++LF+RV   V+ N   K 
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN---KA 310

Query: 310 QKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPP-- 369
            KTV+ AY   FLG+   L++VM + FPELGLT+KDCIE SWI S++Y +G+P+  PP  
Sbjct: 311 NKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPI 370

Query: 370 EVLLQGKSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISE 429
           E+LLQ KS   K YFK KSDF K PIP  GLEG++K+L E+++ L+IW PYGG M KI E
Sbjct: 371 EILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPE 430

Query: 430 TEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDL 489
           +EIPFPHR G  F IQY  +W   E+  ++  +WI++LY YMTPYVS  PR+ YVNYRDL
Sbjct: 431 SEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDL 490

Query: 490 DLGINK-NSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIP 536
           DLG NK NS +++IE+  WG  YFK+NF RL+R+K+KVDPDNFFRHEQSIP +P
Sbjct: 491 DLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLP 541

BLAST of Bhi03G000162 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 651.0 bits (1678), Expect = 8.7e-187
Identity = 307/513 (59.84%), Postives = 393/513 (76.61%), Query Frame = 0

Query: 29  SASLEESFLQCLNENSQFSVPYSSFC---APNNATFNALLQSTAQNLRYLEPSVPKPLFI 88
           SAS+++ F+ C+  N+  S P        A N + FN +L+STAQNL++L  S+PKP FI
Sbjct: 31  SASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKPGFI 90

Query: 89  FTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEIETPFIILDLAKLRSVKVDI 148
           F P+  S VQ+++ICSK+L IH RVRSGGHD+E LSYVS IE PFI+LDL+KL+ + VDI
Sbjct: 91  FRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQINVDI 150

Query: 149 DGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAYGSMMRKYGLGAD 208
           + NSAWVQ GAT+GE+YYRIAEKSK+HGFPAGLCTS+G+GG++TGG YG++MRKYGL  D
Sbjct: 151 ESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGLAGD 210

Query: 209 NVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILWWKLNLVPVPSTVTVFTVAK 268
           NV+D ++VDANG++L+R AMGEDLFWAIRGGGG SFGI+L WK+ LVPVP TVTVFTV K
Sbjct: 211 NVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFTVTK 270

Query: 269 TLEQGA-TKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTVSTAYNALFLGDASRL 328
           TLEQ A  K + KWQQ++ K+ E++ IRV++  A N    G KTV+  Y   FLG+   L
Sbjct: 271 TLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN---DGNKTVTMTYLGQFLGEKGTL 330

Query: 329 LKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQGKSTF-KNYFKAKSDF 388
           LKVM ++FPELGLT+KDC E SWI++ L+  G+P+G+P E+LLQ KS   K+YFKA SDF
Sbjct: 331 LKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKSPLGKDYFKATSDF 390

Query: 389 VKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPFPHRRGVLFKIQYLTTW 448
           VK+PIP  GL+G++KRL E  +  + W PYGGMM KI E+ IPFPHR G LFKI Y   W
Sbjct: 391 VKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFKILYYANW 450

Query: 449 QKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDLGINK-NSNTSYIESIGWGT 508
            + ++   + + WIK++YNYM PYVS  PR+ YVNYRDLD G NK N+  ++IE+  WG 
Sbjct: 451 LENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGP 510

Query: 509 RYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIP 536
           +YFK NF RL+++KTKVDP+NFFRHEQSIPP+P
Sbjct: 511 KYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMP 540

BLAST of Bhi03G000162 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.5e-212
Identity = 348/523 (66.54%), Postives = 439/523 (83.94%), Query Frame = 0

Query: 13  ASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPY-SSFCAPN-NAT-FNALLQSTA 72
           A+LFLV LL  S  + S++L++ F++CL +NS  S P  +SF +P+ NAT F   L+STA
Sbjct: 10  ATLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTA 69

Query: 73  QNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEIETP 132
           QNLRYL PS PKP+FIF PL+++HVQ+AV+C+K+L++HLR+RSGGHDYEGLS+V+E ETP
Sbjct: 70  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 129

Query: 133 FIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHIT 192
           F+I+DL+KLR V VD+D NSAW  AGATIGEVYYRI EKS+ HGFPAGLC+SLG+GGH+ 
Sbjct: 130 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 189

Query: 193 GGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILWWKL 252
           GGAYGSMMRK+GLGADNV+DARIVDANG+IL+R AMGED+FWAIRGGGGGSFG+IL WK+
Sbjct: 190 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 249

Query: 253 NLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTV 312
            LVPVP+TVTVFTV KTLEQ  TK+LYKW+Q+ADKLD+DLFIRVII+ A+   K G +T+
Sbjct: 250 KLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTI 309

Query: 313 STAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQG 372
           S +Y A FLGD++RLL+VM +SFPELGLT+KDC E SWIKSV+YIAG+P+   PE LL G
Sbjct: 310 SMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAG 369

Query: 373 KSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPFPH 432
           KS FKN+FKAKSDFVK+PIP  GLEGLW+R  E++SPL IWNPYGGMM +ISE+EIPFPH
Sbjct: 370 KSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPH 429

Query: 433 RRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDLGINKN 492
           R G LFKIQ+L+TWQ G+ ++++H++WI+++Y+YM  YVS+ PR+ YVNYRDLDLG N+ 
Sbjct: 430 RNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEG 489

Query: 493 SNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIP 533
                 ++  WG +Y+K NF RL+++K + DPDNFFRHEQS+P
Sbjct: 490 ET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Bhi03G000162 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.3e-208
Identity = 345/524 (65.84%), Postives = 425/524 (81.11%), Query Frame = 0

Query: 14  SLFLVFLLSSSSWV--DSASLEESFLQCLNENSQFSVPY-SSFCAP--NNATFNALLQST 73
           +  +  LL S S++    +++++ F+ CL +NS  S P  SSF     N ++F   L+++
Sbjct: 11  AFLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETS 70

Query: 74  AQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEIET 133
           AQNLRYL PS PKP FIF PL+++HVQ+AV+C+K+LK+HLR+RSGGHDYEGLSYVSE+ET
Sbjct: 71  AQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMET 130

Query: 134 PFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHI 193
            F+I+DL+KLR + VDI+ NSAWV AGA+IGEVYYRI EKSK+HGFPAGLCTSLG+GGHI
Sbjct: 131 AFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHI 190

Query: 194 TGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILWWK 253
            GGAYGSMMRK+GLGADNV+DARIVDA+G+ILNR AMGED+FWAIRGGGGGSFG+IL WK
Sbjct: 191 IGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWK 250

Query: 254 LNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKT 313
           + LVPVP  VTVFTV +TLEQ  TK+LYKWQQVADKLDEDLFIRVII   +   K  ++T
Sbjct: 251 IKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERT 310

Query: 314 VSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQ 373
           +ST+Y   FLGDA+RLL+VM  SFP+LGLT+KDC+ETSWIKSV+YIAG+PS  P E LL 
Sbjct: 311 ISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD 370

Query: 374 GKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPFP 433
           GKS FKNYFKAKSD+V++PIP  GLEGLW++L E++SPL IWNPYGGMM KI ETE PFP
Sbjct: 371 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFP 430

Query: 434 HRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDLGINK 493
           HR G LFKIQ+LT WQ G+ ++ KH+ W++++Y+YM  YVS+ PR  YVNYRDLDLG+N 
Sbjct: 431 HRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNG 490

Query: 494 NSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIP 533
             +    ++  WG RYFK NF RL+ +K K DP+NFFRHEQSIP
Sbjct: 491 KGS----DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Bhi03G000162 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 1.0e-200
Identity = 338/531 (63.65%), Postives = 427/531 (80.41%), Query Frame = 0

Query: 10  SFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPY-SSFCAP--NNATFNALLQ 69
           S +   F ++ ++ +S    ASL++ F+ C+  N+    P   +F AP  N + F+ +L+
Sbjct: 11  SILVLYFSLYTITPTS--SLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLE 70

Query: 70  STAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEI 129
           STAQNLR+L+ S+PKP FIF+P+ +SHVQ+++ICSK+L++HLRVRSGGHDYEGLSYVS+I
Sbjct: 71  STAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQI 130

Query: 130 ETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 189
           + PFI++DL+K+R V ++I  NSAWVQ+GAT+GE+YYRIAEKSKVHGFPAGLC+SLG+GG
Sbjct: 131 DKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGG 190

Query: 190 HITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILW 249
           HITGGAYGSMMRKYGLGADNV+DA+IVDANG++L+R AMGED FWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILA 250

Query: 250 WKLNLVPVPSTVTVFTVAKTLEQG-ATKILYKWQQVADKLDEDLFIRVIINVAANADKKG 309
           WK+ LVPVP TVTVFTV KTL+Q    KI+ KWQ+VADKL E+LFIRV+ NVA      G
Sbjct: 251 WKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTG---G 310

Query: 310 QKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEV 369
            KTV+T+YNALFLG    L+ VM +SFPELGLT KDCIE SW++S+ YI+G+P+ TP  V
Sbjct: 311 NKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNV 370

Query: 370 LLQGKSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETE 429
           LLQGKS F K  FKAKSDFVK PIPE+GL+G++K+L +++ PLMIWNPYGGMM KI E++
Sbjct: 371 LLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQ 430

Query: 430 IPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDL 489
           IPFPHR+GVLFK+QY+T+W   ++   +H+ WI+ LY+YMTPYVS  PRE YVNYRDLDL
Sbjct: 431 IPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDL 490

Query: 490 GIN-KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPI 535
           G N K+  T   ++  WG  YFK NF RL+ +K KVDP+NFFRHEQSIPP+
Sbjct: 491 GRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Bhi03G000162 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 3.4e-188
Identity = 321/534 (60.11%), Postives = 409/534 (76.59%), Query Frame = 0

Query: 10  SFVASLFLVFLLSSSSWVDSASLEESFLQCL--NENSQFSVPYSSFCAPNNAT-FNALLQ 69
           S ++   L F   + +   S SL++ F++CL  N N +F++  + F    NA+ F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 70  STAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEI 129
           STAQN RYL  ++PKP FIF P+ +SHVQ++VICSK+L+IH RVRSGGHDYEG+SYVS+I
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 130 ETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGG 189
           E PF+++DL+KLR + VDI   SAWV+AGAT+GE+YYRIAEKSK HGFPAG+  SLG+GG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 190 HITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILW 249
           HITGGAYGS+MRKYGL ADNV+DA+IVDANG++L+R +MGEDLFWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 250 WKLNLVPVPSTVTVFTVAKTLEQGAT-KILYKWQQVADKLDEDLFIRVIINVAANADKKG 309
           WK+ LVPVP T+TVFTV KT EQ  + KIL KWQ++AD L ++LF+RV   V+ N   K 
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN---KA 310

Query: 310 QKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPP-- 369
            KTV+ AY   FLG+   L++VM + FPELGLT+KDCIE SWI S++Y +G+P+  PP  
Sbjct: 311 NKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPI 370

Query: 370 EVLLQGKSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISE 429
           E+LLQ KS   K YFK KSDF K PIP  GLEG++K+L E+++ L+IW PYGG M KI E
Sbjct: 371 EILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPE 430

Query: 430 TEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDL 489
           +EIPFPHR G  F IQY  +W   E+  ++  +WI++LY YMTPYVS  PR+ YVNYRDL
Sbjct: 431 SEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDL 490

Query: 490 DLGINK-NSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIP 536
           DLG NK NS +++IE+  WG  YFK+NF RL+R+K+KVDPDNFFRHEQSIP +P
Sbjct: 491 DLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLP 541

BLAST of Bhi03G000162 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.2e-185
Identity = 307/513 (59.84%), Postives = 393/513 (76.61%), Query Frame = 0

Query: 29  SASLEESFLQCLNENSQFSVPYSSFC---APNNATFNALLQSTAQNLRYLEPSVPKPLFI 88
           SAS+++ F+ C+  N+  S P        A N + FN +L+STAQNL++L  S+PKP FI
Sbjct: 31  SASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKPGFI 90

Query: 89  FTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGLSYVSEIETPFIILDLAKLRSVKVDI 148
           F P+  S VQ+++ICSK+L IH RVRSGGHD+E LSYVS IE PFI+LDL+KL+ + VDI
Sbjct: 91  FRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQINVDI 150

Query: 149 DGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAYGSMMRKYGLGAD 208
           + NSAWVQ GAT+GE+YYRIAEKSK+HGFPAGLCTS+G+GG++TGG YG++MRKYGL  D
Sbjct: 151 ESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGLAGD 210

Query: 209 NVVDARIVDANGRILNREAMGEDLFWAIRGGGGGSFGIILWWKLNLVPVPSTVTVFTVAK 268
           NV+D ++VDANG++L+R AMGEDLFWAIRGGGG SFGI+L WK+ LVPVP TVTVFTV K
Sbjct: 211 NVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFTVTK 270

Query: 269 TLEQGA-TKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTVSTAYNALFLGDASRL 328
           TLEQ A  K + KWQQ++ K+ E++ IRV++  A N    G KTV+  Y   FLG+   L
Sbjct: 271 TLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN---DGNKTVTMTYLGQFLGEKGTL 330

Query: 329 LKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQGKSTF-KNYFKAKSDF 388
           LKVM ++FPELGLT+KDC E SWI++ L+  G+P+G+P E+LLQ KS   K+YFKA SDF
Sbjct: 331 LKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKSPLGKDYFKATSDF 390

Query: 389 VKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKISETEIPFPHRRGVLFKIQYLTTW 448
           VK+PIP  GL+G++KRL E  +  + W PYGGMM KI E+ IPFPHR G LFKI Y   W
Sbjct: 391 VKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFKILYYANW 450

Query: 449 QKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYRDLDLGINK-NSNTSYIESIGWGT 508
            + ++   + + WIK++YNYM PYVS  PR+ YVNYRDLD G NK N+  ++IE+  WG 
Sbjct: 451 LENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGP 510

Query: 509 RYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIP 536
           +YFK NF RL+++KTKVDP+NFFRHEQSIPP+P
Sbjct: 511 KYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMP 540

BLAST of Bhi03G000162 vs. NCBI nr
Match: XP_038882764.1 (berberine bridge enzyme-like 15 [Benincasa hispida])

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 551/551 (100.00%), Postives = 551/551 (100.00%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT
Sbjct: 2   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 61

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 121

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 122 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 181

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS
Sbjct: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 241

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
           ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 302 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 361

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI
Sbjct: 362 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 421

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR
Sbjct: 422 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 481

Query: 481 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL 540
           DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL
Sbjct: 482 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL 541

Query: 541 RSFGSSKRGFD 552
           RSFGSSKRGFD
Sbjct: 542 RSFGSSKRGFD 552

BLAST of Bhi03G000162 vs. NCBI nr
Match: KAA0052471.1 (flavin-dependent oxidoreductase FOX2-like [Cucumis melo var. makuwa] >TYK13351.1 flavin-dependent oxidoreductase FOX2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 534/551 (96.91%), Postives = 541/551 (98.19%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           MV TNPKYKSFVA LFLVFLLSSSSWVDS+SLEESFLQCLNENSQFSVPYSSFCAPNN+T
Sbjct: 2   MVSTNPKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNST 61

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQL+IHLRVRSGGHDYEGL
Sbjct: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGL 121

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SY SE+ETPFIILDLAKLRSVKVDID NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 122 SYASEMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 181

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS
Sbjct: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 241

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
               GQKTVSTAYNALFLGDASRLLKVMGE+FPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 302 G---GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSG 361

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKNYFKAKSDFVK PIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI
Sbjct: 362 TPPEVLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 421

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRE YVNYR
Sbjct: 422 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYR 481

Query: 481 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL 540
           DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQS+PPIPTSE+L
Sbjct: 482 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSVPPIPTSEAL 541

Query: 541 RSFGSSKRGFD 552
           RSFGSSKRGFD
Sbjct: 542 RSFGSSKRGFD 549

BLAST of Bhi03G000162 vs. NCBI nr
Match: XP_008439530.1 (PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo])

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 535/551 (97.10%), Postives = 540/551 (98.00%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           MV TNPKYKSFVA LFLVFLLSSSSWVDS SLEESFLQCLNENSQFSVPYSSFCAPNN+T
Sbjct: 2   MVSTNPKYKSFVAPLFLVFLLSSSSWVDSFSLEESFLQCLNENSQFSVPYSSFCAPNNST 61

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQL+IHLRVRSGGHDYEGL
Sbjct: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGL 121

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SY SE+ETPFIILDLAKLRSVKVDID NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 122 SYASEMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 181

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS
Sbjct: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 241

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
               GQKTVSTAYNALFLGDASRLLKVMGE+FPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 302 G---GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSG 361

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKNYFKAKSDFVK PIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI
Sbjct: 362 TPPEVLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 421

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRE YVNYR
Sbjct: 422 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYR 481

Query: 481 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL 540
           DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSE+L
Sbjct: 482 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEAL 541

Query: 541 RSFGSSKRGFD 552
           RSFGSSKRGFD
Sbjct: 542 RSFGSSKRGFD 549

BLAST of Bhi03G000162 vs. NCBI nr
Match: XP_004134554.1 (berberine bridge enzyme-like 15 [Cucumis sativus] >KAE8647767.1 hypothetical protein Csa_003323 [Cucumis sativus])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 528/551 (95.83%), Postives = 536/551 (97.28%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           MV TNPKYKSFVA LFLVFLLSSSSWVDS+SLEESFLQCLNENSQFSVPYSSFCAPNNAT
Sbjct: 2   MVSTNPKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNAT 61

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FNALLQSTAQNLRYLEPSVPKPLFIFTPL DSHVQSAVICSKQL+IHLRVRSGGHDYEGL
Sbjct: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLVDSHVQSAVICSKQLRIHLRVRSGGHDYEGL 121

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SY SE+ETPFIILDLAKLRS+KVDI+ NSAW QAGATIGEVYYRIAEKSKVHGFPAGLC 
Sbjct: 122 SYASEMETPFIILDLAKLRSIKVDIESNSAWAQAGATIGEVYYRIAEKSKVHGFPAGLCP 181

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS
Sbjct: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 241

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLD+DLFIRVIINVAAN
Sbjct: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDDDLFIRVIINVAAN 301

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
               GQKTVSTAYNALFLGDASRLLKVMGESFPEL LTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 302 G---GQKTVSTAYNALFLGDASRLLKVMGESFPELSLTRKDCIETSWIKSVLYIAGYPSG 361

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKNYFKAKSDFVK+PIPETGLEGLWKRLFEDE PLMIWNPYGGMMGKI
Sbjct: 362 TPPEVLLQGKSTFKNYFKAKSDFVKNPIPETGLEGLWKRLFEDEGPLMIWNPYGGMMGKI 421

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SETEIPFPHRRGVLFKIQYLTTWQK EENQDKHLQWIKKLYNYMTPYVSQLPRE YVNYR
Sbjct: 422 SETEIPFPHRRGVLFKIQYLTTWQKVEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYR 481

Query: 481 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL 540
           DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSE+L
Sbjct: 482 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEAL 541

Query: 541 RSFGSSKRGFD 552
           RSFGSSKRGFD
Sbjct: 542 RSFGSSKRGFD 549

BLAST of Bhi03G000162 vs. NCBI nr
Match: KAG6581484.1 (Berberine bridge enzyme-like 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034774.1 Berberine bridge enzyme-like 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.2e-300
Identity = 508/552 (92.03%), Postives = 535/552 (96.92%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           ++F NPKYKSFV+SLFLVFLLSSS+WV SASL++SFLQCLNENSQFSVPYS+FCAPNNAT
Sbjct: 3   VLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNNAT 62

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FN LLQSTAQNLRYLE SVPKPLFIFTPL DS+VQ+AVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 63  FNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGL 122

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SYVS+IE+PFIILDLAKLRSVKVDID NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 123 SYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 182

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRIL+REAMGEDLFWAIRGGGGGS
Sbjct: 183 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGS 242

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 243 FGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAAN 302

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
            DK+GQKTVSTAYNA FLG+A+RLLK+MGESFPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 303 TDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 362

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKN+FKAKSDFV+DPIPETGLEGLWKRL+E+ESPLMIWNPYGGMM KI
Sbjct: 363 TPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKI 422

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SET+IPFPHRRGVL+KIQYLTTWQK E N+D+HLQWIKKLYNYM PYVSQLPRE YVNYR
Sbjct: 423 SETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYR 482

Query: 481 DLDLGIN-KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 540
           DLDLGIN KNSNTSYIE+IGWGTRYFK+NFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES
Sbjct: 483 DLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 542

Query: 541 LRSFGSSKRGFD 552
           LR FGSS+RGFD
Sbjct: 543 LR-FGSSERGFD 553

BLAST of Bhi03G000162 vs. ExPASy TrEMBL
Match: A0A5A7UG10 (Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009760 PE=3 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 534/551 (96.91%), Postives = 541/551 (98.19%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           MV TNPKYKSFVA LFLVFLLSSSSWVDS+SLEESFLQCLNENSQFSVPYSSFCAPNN+T
Sbjct: 2   MVSTNPKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNST 61

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQL+IHLRVRSGGHDYEGL
Sbjct: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGL 121

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SY SE+ETPFIILDLAKLRSVKVDID NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 122 SYASEMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 181

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS
Sbjct: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 241

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
               GQKTVSTAYNALFLGDASRLLKVMGE+FPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 302 G---GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSG 361

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKNYFKAKSDFVK PIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI
Sbjct: 362 TPPEVLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 421

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRE YVNYR
Sbjct: 422 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYR 481

Query: 481 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL 540
           DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQS+PPIPTSE+L
Sbjct: 482 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSVPPIPTSEAL 541

Query: 541 RSFGSSKRGFD 552
           RSFGSSKRGFD
Sbjct: 542 RSFGSSKRGFD 549

BLAST of Bhi03G000162 vs. ExPASy TrEMBL
Match: A0A1S3AYY2 (flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC103484298 PE=3 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 535/551 (97.10%), Postives = 540/551 (98.00%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           MV TNPKYKSFVA LFLVFLLSSSSWVDS SLEESFLQCLNENSQFSVPYSSFCAPNN+T
Sbjct: 2   MVSTNPKYKSFVAPLFLVFLLSSSSWVDSFSLEESFLQCLNENSQFSVPYSSFCAPNNST 61

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQL+IHLRVRSGGHDYEGL
Sbjct: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEGL 121

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SY SE+ETPFIILDLAKLRSVKVDID NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 122 SYASEMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 181

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS
Sbjct: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 241

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
               GQKTVSTAYNALFLGDASRLLKVMGE+FPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 302 G---GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPSG 361

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKNYFKAKSDFVK PIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI
Sbjct: 362 TPPEVLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 421

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRE YVNYR
Sbjct: 422 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNYR 481

Query: 481 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL 540
           DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSE+L
Sbjct: 482 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEAL 541

Query: 541 RSFGSSKRGFD 552
           RSFGSSKRGFD
Sbjct: 542 RSFGSSKRGFD 549

BLAST of Bhi03G000162 vs. ExPASy TrEMBL
Match: A0A6J1IQ34 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111477806 PE=3 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.6e-300
Identity = 508/552 (92.03%), Postives = 535/552 (96.92%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           ++F NPKYKSFV+SLFLVFLLSSS+WV SASL+ESFLQCLNENSQFSVPYS+FCAPNNAT
Sbjct: 3   VLFINPKYKSFVSSLFLVFLLSSSTWVASASLQESFLQCLNENSQFSVPYSTFCAPNNAT 62

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FN LLQS+AQNLRYLE SVPKPLFIFTPL DS+VQ+AVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 63  FNTLLQSSAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGL 122

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SYVS+IE+PFIILDLAKLRSVKVDID NSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 123 SYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 182

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRIL+REAMGEDLFWAIRGGGGGS
Sbjct: 183 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGS 242

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 243 FGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAAN 302

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
            DKKGQKTVSTAYNA FLG+A+RLLK+MGESFPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 303 TDKKGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 362

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKN+FKAKSDFV+DPIPETGLEGLWKRL+E+ESPLMIWNPYGGMM KI
Sbjct: 363 TPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKI 422

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SET+IPFPHRRGVL+KIQYLTTWQK E N+D+HLQWIKKLYNYM PYVSQLPRE YVNYR
Sbjct: 423 SETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYR 482

Query: 481 DLDLGIN-KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 540
           DLDLGIN KNSNTSYIE+IGWGTRYF++NFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES
Sbjct: 483 DLDLGINEKNSNTSYIEAIGWGTRYFRQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 542

Query: 541 LRSFGSSKRGFD 552
           LR FGSS+RGFD
Sbjct: 543 LR-FGSSERGFD 553

BLAST of Bhi03G000162 vs. ExPASy TrEMBL
Match: A0A6J1ECW5 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111433029 PE=3 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 4.5e-300
Identity = 507/552 (91.85%), Postives = 534/552 (96.74%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           ++F NPKYKSFV+SLFLVFLLSSS+WV SASL++SFLQCLNENSQFSVPYS+FCAPNNAT
Sbjct: 3   VLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNNAT 62

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FN LLQSTAQNLRYLE SVPKPLFIFTPL DS+VQ+AVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 63  FNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGL 122

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SYVS+IE+PFIILDLAKLRSVKVDID NSAWVQAGATIGEVYYRI EKSKVHGFPAGLCT
Sbjct: 123 SYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRITEKSKVHGFPAGLCT 182

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRIL+REAMGEDLFWAIRGGGGGS
Sbjct: 183 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGS 242

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 243 FGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAAN 302

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
            DK+GQKTVSTAYNA FLG+A+RLLK+MGESFPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 303 TDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 362

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKN+FKAKSDFV+DPIPETGLEGLWKRL+E+ESPLMIWNPYGGMM KI
Sbjct: 363 TPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKI 422

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SET+IPFPHRRGVL+KIQYLTTWQK E N+D+HLQWIKKLYNYM PYVSQLPRE YVNYR
Sbjct: 423 SETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYR 482

Query: 481 DLDLGIN-KNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 540
           DLDLGIN KNSNTSYIE+IGWGTRYFK+NFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES
Sbjct: 483 DLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 542

Query: 541 LRSFGSSKRGFD 552
           LR FGSS+RGFD
Sbjct: 543 LR-FGSSERGFD 553

BLAST of Bhi03G000162 vs. ExPASy TrEMBL
Match: A0A6J1CYZ1 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111015758 PE=3 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 6.5e-299
Identity = 508/551 (92.20%), Postives = 526/551 (95.46%), Query Frame = 0

Query: 1   MVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 60
           MVF  PKYKS V SLFLVFLLSSS WVDSASLEESFLQCLNENSQFSVP+SSFCAPNNAT
Sbjct: 1   MVF--PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNAT 60

Query: 61  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEGL 120
           FNALLQSTAQNLRYLE SVPKPLFIFTP +DSHVQ+AVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 61  FNALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGL 120

Query: 121 SYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180
           SY SE ET F+ILDLAKLRS+KVDI+GNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT
Sbjct: 121 SYASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180

Query: 181 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGGS 240
           SLGVGGHITGGAYG MMRKYGLGADNVVDARIVDANGRIL+REAMGED+FWAIRGGGGGS
Sbjct: 181 SLGVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGS 240

Query: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300

Query: 301 ADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 360
           ADKK  KTVSTAYNALFLGDA+RLLKVMGESFPELGLTRKDCIE SWIKSVLYIAG+PSG
Sbjct: 301 ADKK--KTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSG 360

Query: 361 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGKI 420
           TPPEVLLQGKSTFKNYFKAKSDFVK+PI ETGLEGLWKRLFE+ESPL IWNPYGGMMGKI
Sbjct: 361 TPPEVLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKI 420

Query: 421 SETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNYR 480
           SETEIPFPHRRG+LFKIQYLT WQKG+ NQ+KHL+WIKKLYNYMTPYVSQLPRE YVNYR
Sbjct: 421 SETEIPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYR 480

Query: 481 DLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSESL 540
           DLDLGINK  NTSYI++IGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTS S 
Sbjct: 481 DLDLGINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASF 540

Query: 541 RSFGSSKRGFD 552
           RSFGSS R FD
Sbjct: 541 RSFGSSSREFD 547

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G34790.11.1e-21366.54FAD-binding Berberine family protein [more]
AT1G30760.11.6e-20965.84FAD-binding Berberine family protein [more]
AT5G44400.17.3e-20263.65FAD-binding Berberine family protein [more]
AT5G44390.12.4e-18960.11FAD-binding Berberine family protein [more]
AT5G44380.18.7e-18759.84FAD-binding Berberine family protein [more]
Match NameE-valueIdentityDescription
O647431.5e-21266.54Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV... [more]
Q93ZA32.3e-20865.84Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=... [more]
Q9FKU81.0e-20063.65Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... [more]
Q9FKU93.4e-18860.11Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=... [more]
Q9FKV01.2e-18559.84Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=... [more]
Match NameE-valueIdentityDescription
XP_038882764.10.0e+00100.00berberine bridge enzyme-like 15 [Benincasa hispida][more]
KAA0052471.10.0e+0096.91flavin-dependent oxidoreductase FOX2-like [Cucumis melo var. makuwa] >TYK13351.1... [more]
XP_008439530.10.0e+0097.10PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo][more]
XP_004134554.10.0e+0095.83berberine bridge enzyme-like 15 [Cucumis sativus] >KAE8647767.1 hypothetical pro... [more]
KAG6581484.13.2e-30092.03Berberine bridge enzyme-like 15, partial [Cucurbita argyrosperma subsp. sororia]... [more]
Match NameE-valueIdentityDescription
A0A5A7UG100.0e+0096.91Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3AYY20.0e+0097.10flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC10348429... [more]
A0A6J1IQ341.6e-30092.03berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111477806 PE=3... [more]
A0A6J1ECW54.5e-30091.85berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111433029 PE... [more]
A0A6J1CYZ16.5e-29992.20berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111015758 P... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 476..532
e-value: 8.5E-22
score: 77.0
NoneNo IPR availableGENE3D3.40.462.20coord: 257..471
e-value: 8.6E-171
score: 570.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 530..544
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 527..551
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 24..537
NoneNo IPR availablePANTHERPTHR32448:SF129TETRAHYDROCANNABINOLIC ACID SYNTHASE-LIKEcoord: 24..537
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 85..220
e-value: 1.2E-25
score: 89.9
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 142..533
e-value: 8.6E-171
score: 570.5
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 25..138
e-value: 1.8E-42
score: 145.5
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 78..254
score: 16.717447
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 55..255

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000162Bhi03M000162mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding