Bhi02G001514 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G001514
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr2: 49275504 .. 49276982 (-)
RNA-Seq Expression	Bhi02G001514
Synteny	Bhi02G001514

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGTTATAAATTCAACTATTATCTATCATACATACCACGAAACGATATGTATCCATTTTTTTTTTTTCAGTTTTCCCTACAACCAGACATGCCTTCATAATCTATTATATAACTCTATTCTTTTGGTAATGGCTCCCATTTGAAATTGGATTTTAAATCAATCACATTAGAAAAAATGGCAAAGCTTTCCCACATAATTGTCATCTTCATGGTTTTCGTGTCATCAATCGTCGGAGCATACGATGTTCTTCTCGGTGCTTACTATAGACTTGCTGGTAACAACCTGCCACCACCATGGGAGGTGGTGCAACTCTGTAAAAAATACAACATTCGTCGCGTTCGATTGTATGAACCCAACCTCACCGTTCTTGAATCATTTCGTGGCATGGGAATCGACGTCTCTTTCGGTGTGCCCAACAACTTGATTGAAAACATGGCCACCAATCACACCGCGGTGGAGGAGTGGTTTATCAACTATGTCGCACCATTCATTGGCGATTTTACCATCAACTACATCGTTGTTGGTGACAAGGTCATCCCTGGACTCGATGGCAGGATATTGCAAGTCATGCAATCCCTTCAAGGTCTCCTTAATGCTCGTAACCTCGGACAAGTGAAACTCACCACATTGGTTTCCATAACTGCATTGTCCGCTCAAAGCCCTCCATCTAGTGGCGCATTTGACCCAACCATTGCTGCAAACATGAGAGGAATCCTCCACTTCTTATGGGAACAGGGGTCACCGTTGATGGTTAGCTTATATCCGCATAAAACATATTTTTACAGTGGCAATATGAGTTTGGGTTATGCGACATTCGCTGAAGAAAACTTTTCGATTCGTGATGGGGACTTAAGTTATAACAACTTGTTTGATGAAATGATGGATGCATTTTATGCGGCGATCGATAAGGAAGATGTTGGAGATGTGGCCATTGCGGTGGGGGAAACTGGGTGGCCGACTTGTGGGCATCTTAACATTACAACAACATCGATTGCAGCTACATATAATCGAAATTTTAAAAATCATATTACCTCTGGCAAGGGGACACCAATGAAGTCTAATATTTATATTCAAGGATTCATCAAGAGTATATTCAACGAGAATGAAGAGCCTGAAGGGGAGTCGAAGTGTTATGGCATGTTCCATGTTGATTCCACACCTATTTACTCGCCTGTGTTTTAATTGGATTGTTAGAGAAAATGTCACATCAACAATCAAGGAAGAGTCAAGAAGACACATCAGCTGAAGGTTGTCATATCACTCTGGAGAGTCATGAAGAATTATTCTTTTTAATGCTCCAAATTTCTGTTAGGATTGGTGATAACAAACAAATATTATTTCTTCATTTATTGAACCATGTGTCCTCATTTTATTAATTGTTTAATATATATATTTTTAAATCTTTGAATCTCCAAAATAGGAAACAGTGTATAAATCGGGCATGTTGCCACTTTTCAATGAATATGAAGAATACGAA

mRNA sequence

GTTGTTATAAATTCAACTATTATCTATCATACATACCACGAAACGATATGTATCCATTTTTTTTTTTTCAGTTTTCCCTACAACCAGACATGCCTTCATAATCTATTATATAACTCTATTCTTTTGGTAATGGCTCCCATTTGAAATTGGATTTTAAATCAATCACATTAGAAAAAATGGCAAAGCTTTCCCACATAATTGTCATCTTCATGGTTTTCGTGTCATCAATCGTCGGAGCATACGATGTTCTTCTCGGTGCTTACTATAGACTTGCTGGTAACAACCTGCCACCACCATGGGAGGTGGTGCAACTCTGTAAAAAATACAACATTCGTCGCGTTCGATTGTATGAACCCAACCTCACCGTTCTTGAATCATTTCGTGGCATGGGAATCGACGTCTCTTTCGGTGTGCCCAACAACTTGATTGAAAACATGGCCACCAATCACACCGCGGTGGAGGAGTGGTTTATCAACTATGTCGCACCATTCATTGGCGATTTTACCATCAACTACATCGTTGTTGGTGACAAGGTCATCCCTGGACTCGATGGCAGGATATTGCAAGTCATGCAATCCCTTCAAGGTCTCCTTAATGCTCGTAACCTCGGACAAGTGAAACTCACCACATTGGTTTCCATAACTGCATTGTCCGCTCAAAGCCCTCCATCTAGTGGCGCATTTGACCCAACCATTGCTGCAAACATGAGAGGAATCCTCCACTTCTTATGGGAACAGGGGTCACCGTTGATGGTTAGCTTATATCCGCATAAAACATATTTTTACAGTGGCAATATGAGTTTGGGTTATGCGACATTCGCTGAAGAAAACTTTTCGATTCGTGATGGGGACTTAAGTTATAACAACTTGTTTGATGAAATGATGGATGCATTTTATGCGGCGATCGATAAGGAAGATGTTGGAGATGTGGCCATTGCGGTGGGGGAAACTGGGTGGCCGACTTGTGGGCATCTTAACATTACAACAACATCGATTGCAGCTACATATAATCGAAATTTTAAAAATCATATTACCTCTGGCAAGGGGACACCAATGAAGTCTAATATTTATATTCAAGGATTCATCAAGAGTATATTCAACGAGAATGAAGAGCCTGAAGGGGAGTCGAAGTGTTATGGCATGTTCCATGTTGATTCCACACCTATTTACTCGCCTGTGTTTTAATTGGATTGTTAGAGAAAATGTCACATCAACAATCAAGGAAGAGTCAAGAAGACACATCAGCTGAAGGTTGTCATATCACTCTGGAGAGTCATGAAGAATTATTCTTTTTAATGCTCCAAATTTCTGTTAGGATTGGTGATAACAAACAAATATTATTTCTTCATTTATTGAACCATGTGTCCTCATTTTATTAATTGTTTAATATATATATTTTTAAATCTTTGAATCTCCAAAATAGGAAACAGTGTATAAATCGGGCATGTTGCCACTTTTCAATGAATATGAAGAATACGAA

Coding sequence (CDS)

ATGGCAAAGCTTTCCCACATAATTGTCATCTTCATGGTTTTCGTGTCATCAATCGTCGGAGCATACGATGTTCTTCTCGGTGCTTACTATAGACTTGCTGGTAACAACCTGCCACCACCATGGGAGGTGGTGCAACTCTGTAAAAAATACAACATTCGTCGCGTTCGATTGTATGAACCCAACCTCACCGTTCTTGAATCATTTCGTGGCATGGGAATCGACGTCTCTTTCGGTGTGCCCAACAACTTGATTGAAAACATGGCCACCAATCACACCGCGGTGGAGGAGTGGTTTATCAACTATGTCGCACCATTCATTGGCGATTTTACCATCAACTACATCGTTGTTGGTGACAAGGTCATCCCTGGACTCGATGGCAGGATATTGCAAGTCATGCAATCCCTTCAAGGTCTCCTTAATGCTCGTAACCTCGGACAAGTGAAACTCACCACATTGGTTTCCATAACTGCATTGTCCGCTCAAAGCCCTCCATCTAGTGGCGCATTTGACCCAACCATTGCTGCAAACATGAGAGGAATCCTCCACTTCTTATGGGAACAGGGGTCACCGTTGATGGTTAGCTTATATCCGCATAAAACATATTTTTACAGTGGCAATATGAGTTTGGGTTATGCGACATTCGCTGAAGAAAACTTTTCGATTCGTGATGGGGACTTAAGTTATAACAACTTGTTTGATGAAATGATGGATGCATTTTATGCGGCGATCGATAAGGAAGATGTTGGAGATGTGGCCATTGCGGTGGGGGAAACTGGGTGGCCGACTTGTGGGCATCTTAACATTACAACAACATCGATTGCAGCTACATATAATCGAAATTTTAAAAATCATATTACCTCTGGCAAGGGGACACCAATGAAGTCTAATATTTATATTCAAGGATTCATCAAGAGTATATTCAACGAGAATGAAGAGCCTGAAGGGGAGTCGAAGTGTTATGGCATGTTCCATGTTGATTCCACACCTATTTACTCGCCTGTGTTTTAA

Protein sequence

MAKLSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF

Homology

[Close]

BLAST of Bhi02G001514 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 239.2 bits (609), Expect = 4.8e-63
Identity = 127/311 (40.84%), Postives = 194/311 (62.38%), Query Frame = 0


Query: 26  LGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLESFRG-MGIDVSFGVPNNLI 85
           +G  Y L G+NLP P +V+ L K  +I ++R+++PN  VL + RG   I V+ GV +  +
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 86  ENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLDG-RILQVMQSLQGLLNARN 145
             ++ +  AV+ WF   + P++ D  I +I VG++VIPG  G ++L VMQSL  L+ +RN
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 146 LGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLWEQGSPLMVSLYPHKTYFY 205
           L  + ++T+V++  L    PPS+G F       +  +L  L +  SP++V +YP+ +Y  
Sbjct: 163 L-PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYAS 222

Query: 206 S-GNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDKEDVGDVAIAVGETGWPT 265
              ++ L YATF  E   ++DG L Y+N+FD + DAF  A++KE V D+ + V ETGWP+
Sbjct: 223 DPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPS 282

Query: 266 CGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKSIFNENEEPEGESKCYGM 325
            G+ NITT  IA TYNRNF  HI SGKGTP + N  I GF+ + FNEN++P G  + +G+
Sbjct: 283 AGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFGL 342

Query: 326 FHV-DSTPIYS 333
           ++  D  PIY+
Sbjct: 343 YNPNDMKPIYN 352

[Close]

BLAST of Bhi02G001514 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 236.1 bits (601), Expect = 4.1e-62
Identity = 123/310 (39.68%), Postives = 194/310 (62.58%), Query Frame = 0


Query: 26  LGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLESFRG-MGIDVSFGVPNNLI 85
           +G  Y L G+NLP P  V+ L K   I R+R+++PN  VL + RG   I+V+ GV +  +
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 86  ENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLDG-RILQVMQSLQGLLNARN 145
             +A +  AV+ WF   +  ++ D  I +I VG++VIPG  G ++L VMQSL  L+ +RN
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 146 LGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLWEQGSPLMVSLYPHKTYFY 205
           L  + ++T+V+++ L    PPS+G F       +  +L  L +  +P++V++YP+  Y  
Sbjct: 154 L-PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYAS 213

Query: 206 S-GNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDKEDVGDVAIAVGETGWPT 265
              N+ L YA+F  ++  ++DG L Y+N+FD + DAF  A++KE V ++ + V ETGWP+
Sbjct: 214 DPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWPS 273

Query: 266 CGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKSIFNENEEPEGESKCYGM 325
            G+ N TT +IA+TYNRNF  HI SGKGTP + N  + GF+ + FNEN++P G  + +G+
Sbjct: 274 AGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFGL 333

Query: 326 FH-VDSTPIY 332
           ++  D  PIY
Sbjct: 334 YNPSDMKPIY 342

[Close]

BLAST of Bhi02G001514 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.7e-60
Identity = 127/334 (38.02%), Postives = 198/334 (59.28%), Query Frame = 0


Query: 4   LSHIIVIFMVFVSSIVGAYDVL--LGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPN 63
           L  + V F++  S+ VG  D    +G  Y L G+NLPPP EV+ L K  ++  +R+++  
Sbjct: 10  LKFLCVAFLLNYSN-VGFVDAATNIGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTT 69

Query: 64  LTVLESFRG-MGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKV 123
             VL +FRG   I V   V N  +E ++ +  AV  WF+  + P++ D  I +I VG++V
Sbjct: 70  TDVLNAFRGNRNIGVMVDVKNQDLEALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEV 129

Query: 124 IPG-LDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRG 183
           IPG +   +L VM+SL  ++ +R+L  + ++T V++T L    PPS+G F P     +  
Sbjct: 130 IPGEIGSYVLPVMKSLTNIVKSRSL-PILISTTVAMTNLGQSYPPSAGDFMPQAREQLTP 189

Query: 184 ILHFLWEQGSPLMVSLYPHKTYFYSG-NMSLGYATFAEENFSIRDGDLSYNNLFDEMMDA 243
           +L FL +  +P++V++YP+  Y     N+ L YA F      ++DG L Y N+FD + DA
Sbjct: 190 VLKFLSQTNTPILVNIYPYFAYAADPINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDA 249

Query: 244 FYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIY 303
           F  A++KE V D+ + V ETGWP+ G+ N+TT  IA+ YN NF  H+ SGKGTP +    
Sbjct: 250 FVWAMEKEGVKDLPMVVTETGWPSAGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSG 309

Query: 304 IQGFIKSIFNENEEPEGESKCYGMFH-VDSTPIY 332
           I GF+ + FNEN++P G  + +G+++  D  PIY
Sbjct: 310 ISGFLFATFNENQKPAGTEQNFGLYNPTDMKPIY 341

[Close]

BLAST of Bhi02G001514 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 6.1e-58
Identity = 116/305 (38.03%), Postives = 183/305 (60.00%), Query Frame = 0


Query: 25  LLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLESFRG-MGIDVSFGVPNNL 84
           ++G  Y L G+NLP P  V++  K  N+ ++R++EPN  VL + RG   I V+ G+ N  
Sbjct: 33  VVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNED 92

Query: 85  IENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPG--LDGRILQVMQSLQGLLNA 144
           +E +A N  AV+ WF   + P+I D  I +I VG++ IPG      +L V+QSL  L+ +
Sbjct: 93  LEALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKS 152

Query: 145 RNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLWEQGSPLMVSLYPHKTY 204
           RNL Q+ ++T V+ T+L+   PPS+G   P     +  +L  L +  +P+ V++YP+  Y
Sbjct: 153 RNL-QISISTTVTTTSLARLKPPSAGVLTPQARQQLVPVLRLLSQTSTPIFVNIYPY--Y 212

Query: 205 FYSG---NMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDKEDVGDVAIAVGET 264
           F++    N+ L YA F  +   ++DG L Y+NLFD + DAF  A++KE V  + + V ET
Sbjct: 213 FHASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSET 272

Query: 265 GWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKSIFNENEEPEGESK 324
           GWP+ G+  +TT ++  TY  NF  H+ SGKGTP + N  I  +I   +NEN++P G  +
Sbjct: 273 GWPSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKRPNSRIDAYIFETYNENQKPVGIYQ 332

[Close]

BLAST of Bhi02G001514 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.0e-57
Identity = 128/331 (38.67%), Postives = 194/331 (58.61%), Query Frame = 0


Query: 4   LSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLT 63
           LS I++ F    S  V A +  +G  Y L G+N P P  V+ L K   I ++R+++P   
Sbjct: 13  LSCIMLTFNYNTSGFVAAAN-SIGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTE 72

Query: 64  VLESFRG-MGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIP 123
           VL + RG   I+V+ GV +  +  ++ N  AV+ WF   + P++ D  I +I VG++VIP
Sbjct: 73  VLNALRGHRNIEVTVGVRDQDLGALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIP 132

Query: 124 GLDG-RILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGIL 183
           G  G ++L VMQSL  L+ + NL  + ++T+V+++ L    PPS+G F       +  +L
Sbjct: 133 GPIGPQVLPVMQSLTILVKSMNL-PISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVL 192

Query: 184 HFLWEQGSPLMVSLYPHKTYFYS-GNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFY 243
             L +  +P++V++YP+  Y     N+ L YATF  +   ++DG L Y+N+FD + DAF 
Sbjct: 193 KLLSQTSTPILVNIYPYFPYASDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFV 252

Query: 244 AAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQ 303
            A++KE V D+ + V ETGWP+ G+ N+TT  IA TYNRNF  HI SGKGTP + N  + 
Sbjct: 253 WAMEKEGVKDLPMVVSETGWPSAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMD 312

Query: 304 GFIKSIFNENEEPEGESKCYGMFHVDSTPIY 332
           GF+ + FNEN++P G          D  PIY
Sbjct: 313 GFLFATFNENQKPAG---------TDMKPIY 332

[Close]

BLAST of Bhi02G001514 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.8e-62
Identity = 127/311 (40.84%), Postives = 194/311 (62.38%), Query Frame = 0


Query: 26  LGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLESFRG-MGIDVSFGVPNNLI 85
           +G  Y L G+NLP P +V+ L K  +I ++R+++PN  VL + RG   I V+ GV +  +
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 86  ENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLDG-RILQVMQSLQGLLNARN 145
             ++ +  AV+ WF   + P++ D  I +I VG++VIPG  G ++L VMQSL  L+ +RN
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 146 LGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLWEQGSPLMVSLYPHKTYFY 205
           L  + ++T+V++  L    PPS+G F       +  +L  L +  SP++V +YP+ +Y  
Sbjct: 163 L-PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYAS 222

Query: 206 S-GNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDKEDVGDVAIAVGETGWPT 265
              ++ L YATF  E   ++DG L Y+N+FD + DAF  A++KE V D+ + V ETGWP+
Sbjct: 223 DPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPS 282

Query: 266 CGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKSIFNENEEPEGESKCYGM 325
            G+ NITT  IA TYNRNF  HI SGKGTP + N  I GF+ + FNEN++P G  + +G+
Sbjct: 283 AGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFGL 342

Query: 326 FHV-DSTPIYS 333
           ++  D  PIY+
Sbjct: 343 YNPNDMKPIYN 352

[Close]

BLAST of Bhi02G001514 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.7e-61
Identity = 123/310 (39.68%), Postives = 194/310 (62.58%), Query Frame = 0


Query: 26  LGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLESFRG-MGIDVSFGVPNNLI 85
           +G  Y L G+NLP P  V+ L K   I R+R+++PN  VL + RG   I+V+ GV +  +
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 86  ENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLDG-RILQVMQSLQGLLNARN 145
             +A +  AV+ WF   +  ++ D  I +I VG++VIPG  G ++L VMQSL  L+ +RN
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 146 LGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLWEQGSPLMVSLYPHKTYFY 205
           L  + ++T+V+++ L    PPS+G F       +  +L  L +  +P++V++YP+  Y  
Sbjct: 154 L-PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYAS 213

Query: 206 S-GNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDKEDVGDVAIAVGETGWPT 265
              N+ L YA+F  ++  ++DG L Y+N+FD + DAF  A++KE V ++ + V ETGWP+
Sbjct: 214 DPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWPS 273

Query: 266 CGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKSIFNENEEPEGESKCYGM 325
            G+ N TT +IA+TYNRNF  HI SGKGTP + N  + GF+ + FNEN++P G  + +G+
Sbjct: 274 AGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFGL 333

Query: 326 FH-VDSTPIY 332
           ++  D  PIY
Sbjct: 334 YNPSDMKPIY 342

[Close]

BLAST of Bhi02G001514 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 9.4e-56
Identity = 118/314 (37.58%), Postives = 183/314 (58.28%), Query Frame = 0


Query: 26  LGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLESFRGMGIDVSFGVPNNLIE 85
           +G  Y + GNNLPPP EVV L K  NI R+RLY+PN   L++ R   I V   VP + ++
Sbjct: 29  IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQ 88

Query: 86  NMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLD--GRILQVMQSLQGLLNARN 145
           ++A+N +A  +W    V  +    +  YI VG+++IPG D    IL  M+++   L++  
Sbjct: 89  SLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAG 148

Query: 146 L-GQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLWEQGSPLMVSLYPHKTYF 205
           L  Q+K++T V    L    PPS+GAF     A +  I+ FL   G+PL+V++YP+ +Y 
Sbjct: 149 LQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSYT 208

Query: 206 YS-GNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDKEDVGDVAIAVGETGWP 265
            + G +SL YA F      ++DG  SY NLFD ++DA +AA+++    +VA+ V E+GWP
Sbjct: 209 GNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGWP 268

Query: 266 TCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKSIFNENEEPEGESKCYG 325
           + G     +TS A TYN+N   H+  G GTP +    I+ +I  +FNEN++  G  + +G
Sbjct: 269 SAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQNFG 328

Query: 326 MFHVDSTPIYSPVF 336
           +F+ +  P+Y   F
Sbjct: 329 LFYPNKQPVYQISF 340

[Close]

BLAST of Bhi02G001514 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 202.2 bits (513), Expect = 9.1e-51
Identity = 121/334 (36.23%), Postives = 189/334 (56.59%), Query Frame = 0


Query: 7   IIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLE 66
           I++  +V  + IVGA  V  G  Y + GNNLPP  +VVQL K  NIRR+RLY+PN   L+
Sbjct: 16  ILLGLLVSSTEIVGAQSV--GVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQ 75

Query: 67  SFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIP---- 126
           + RG  I+V  GVPN+ ++N+A N +    W    V  F       YI VG++V P    
Sbjct: 76  ALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGT 135

Query: 127 -GLDGRILQVMQSLQGLLNARNL-GQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGI 186
             L   +L  M++++  +++  L   +K+++ V +T +    PPS G+F   + + +  I
Sbjct: 136 SSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDPI 195

Query: 187 LHFLWEQGSPLMVSLYPHKTYFYSGN---MSLGYATFAEENFSIRDGDLSYNNLFDEMMD 246
           + F+    SPL+V++YP+  + Y+GN   +SL YA F   N  ++DG L Y NLFD M D
Sbjct: 196 IGFVRRINSPLLVNIYPY--FSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSD 255

Query: 247 AFYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNI 306
           A YAA+ +   G + I V E+GWP+ G    TT + AATY +N   H+   +G+P + N 
Sbjct: 256 AVYAALSRAGGGSIEIVVSESGWPSAGAFAATTNN-AATYYKNLIQHVK--RGSPRRPNK 315

Query: 307 YIQGFIKSIFNENEEPEGESKCYGMFHVDSTPIY 332
            I+ ++ ++F+EN +     K +G+F  +  P Y
Sbjct: 316 VIETYLFAMFDENNKNPELEKHFGLFSPNKQPKY 342

[Close]

BLAST of Bhi02G001514 vs. ExPASy Swiss-Prot
Match: P12257 (Lichenase-2 (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 9.5e-48
Identity = 107/307 (34.85%), Postives = 170/307 (55.37%), Query Frame = 0


Query: 26  LGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVLESFRGMGIDVSFGVPNNLIE 85
           +G  Y ++ NNLP    VV + K   I+ +RLY PN   L++  G GI+V  G PN+++ 
Sbjct: 7   IGVCYGMSANNLPAASTVVSMFKFNGIKSMRLYAPNQAALQAVGGTGINVVVGAPNDVLS 66

Query: 86  NMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLDGRILQVMQSLQGLLNARNLG 145
           N+A +  A   W  + +  +    +  Y+ VG++V  G    ++  M+++ G L A  LG
Sbjct: 67  NLAASPAAAASWVKSNIQAY-PKVSFRYVCVGNEVAGGATRNLVPAMKNVHGALVAAGLG 126

Query: 146 QVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLWEQGSPLMVSLYPHKTYFYSG 205
            +K+TT VS   L   SPPS+G+F    AA M  ++ FL    +PLM ++YP+  + Y+ 
Sbjct: 127 HIKVTTSVSQAILGVFSPPSAGSFTGEAAAFMGPVVQFLARTNAPLMANIYPYLAWAYNP 186

Query: 206 N-MSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFYAAIDKEDVGDVAIAVGETGWPTCG 265
           + M +GYA F      +RDG   Y NLFD  +DAFY A+ K     V + V E+GWP+ G
Sbjct: 187 SAMDMGYALFNASGTVVRDGAYGYQNLFDTTVDAFYTAMGKHGGSSVKLVVSESGWPSGG 246

Query: 266 HLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQGFIKSIFNENEEPEGESKCYGMFH 325
                T + A  YN++  NH+  G+GTP      I+ +I ++FNEN++  G  + +G+F+
Sbjct: 247 G-TAATPANARFYNQHLINHV--GRGTPRHPGA-IETYIFAMFNENQKDSGVEQNWGLFY 306

Query: 326 VDSTPIY 332
            +   +Y
Sbjct: 307 PNMQHVY 308

[Close]

BLAST of Bhi02G001514 vs. NCBI nr
Match: XP_038880685.1 (probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida])

HSP 1 Score: 677.9 bits (1748), Expect = 4.3e-191
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0


Query: 1   MAKLSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEP 60
           MAKLSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEP
Sbjct: 1   MAKLSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEP 60

Query: 61  NLTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKV 120
           NLTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKV
Sbjct: 61  NLTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKV 120

Query: 121 IPGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGI 180
           IPGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGI
Sbjct: 121 IPGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGI 180

Query: 181 LHFLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFY 240
           LHFLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFY
Sbjct: 181 LHFLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFY 240

Query: 241 AAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQ 300
           AAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQ
Sbjct: 241 AAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQ 300

Query: 301 GFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
           GFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF
Sbjct: 301 GFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 335

[Close]

BLAST of Bhi02G001514 vs. NCBI nr
Match: TYK10032.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 471.9 bits (1213), Expect = 4.7e-129
Identity = 240/337 (71.22%), Postives = 281/337 (83.38%), Query Frame = 0


Query: 5   SHII-VIFMVFVSSI-VGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNL 64
           SH+I ++FMV +SSI VGAY++LLGAYY L  NNLP PW+V+QLC+KYNIRR+R+ EPNL
Sbjct: 6   SHVIALLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNL 65

Query: 65  TVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIP 124
            V+ESFRG  I+VSFGV N LI NMATNHTAVEEWF NY+APFI D TINYIVVGDK +P
Sbjct: 66  DVIESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVP 125

Query: 125 GLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILH 184
           GLD  IL VM+SLQ LLN R LGQVK+TTLV ++AL  ++PPSSGAFDP +  NMRGIL 
Sbjct: 126 GLDNSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENMRGILQ 185

Query: 185 FLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFA---EENFSIR-DGDLSYNNLFDEMMDA 244
           FLW QGSPLM+SL P++ Y Y+ N+SLGYATF    E+N  IR DG+LSYNNLFDE++DA
Sbjct: 186 FLWGQGSPLMLSLNPYEAYAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDA 245

Query: 245 FYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIY 304
           FYAAIDK  VG VAIA+GETGWPT G  +  +  +AATYN NFKNHI+SGKGTPMK NIY
Sbjct: 246 FYAAIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIY 305

Query: 305 IQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
           I+GFIKS+FNENE+PEGES+ YGMF+VDSTPIYSPVF
Sbjct: 306 IEGFIKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of Bhi02G001514 vs. NCBI nr
Match: KAA0055781.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 471.5 bits (1212), Expect = 6.2e-129
Identity = 241/337 (71.51%), Postives = 281/337 (83.38%), Query Frame = 0


Query: 5   SHII-VIFMVFVSSIV-GAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNL 64
           SH+I ++FMV +SSIV GAY+VLLGAYY L  NNLP PW+V+QLC+KYNIRR+R+ EPNL
Sbjct: 6   SHVIALLFMVDMSSIVLGAYNVLLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNL 65

Query: 65  TVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIP 124
            V+ESFRG  I+VSFGV N LI NMATNHTAVEEWF NY+APFI D TINYIVVGDK +P
Sbjct: 66  DVIESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVP 125

Query: 125 GLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILH 184
           GLD  IL VM+SLQ LLN R LGQVK+TTLV ++AL  ++PPSSGAFDP +  NMRGIL 
Sbjct: 126 GLDDSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENMRGILQ 185

Query: 185 FLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFA---EENFSIR-DGDLSYNNLFDEMMDA 244
           FLW QGSPLM+SL P++ Y Y+ N+SLGYATF    E+N  IR DG+LSYNNLFDE++DA
Sbjct: 186 FLWGQGSPLMLSLNPYEAYAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDA 245

Query: 245 FYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIY 304
           FYAAIDK  VG VAIA+GETGWPT G  +  +  +AATYN NFKNHI+SGKGTPMK NIY
Sbjct: 246 FYAAIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIY 305

Query: 305 IQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
           I+GFIKS+FNENE+PEGES+ YGMF+VDSTPIYSPVF
Sbjct: 306 IEGFIKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of Bhi02G001514 vs. NCBI nr
Match: XP_031742611.1 (glucan endo-1,3-beta-glucosidase-like [Cucumis sativus] >XP_031742618.1 glucan endo-1,3-beta-glucosidase-like [Cucumis sativus] >KAE8653447.1 hypothetical protein Csa_007612 [Cucumis sativus] >KAE8653450.1 hypothetical protein Csa_007638 [Cucumis sativus])

HSP 1 Score: 468.4 bits (1204), Expect = 5.2e-128
Identity = 238/337 (70.62%), Postives = 274/337 (81.31%), Query Frame = 0


Query: 7   IIVIFMVFVSSI-VGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNLTVL 66
           I ++FMV +SSI V   ++L+GAYY   GNNLPPPW+VVQLC+KYNIRR+R  EPNL +L
Sbjct: 9   IALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDIL 68

Query: 67  ESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIPGLD 126
           E FRG  I+VSF VP  LI N ATNHTAVEEWF+ YV PFIG+FTINYIVVGDKVIPGLD
Sbjct: 69  EQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLD 128

Query: 127 GRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILHFLW 186
             IL VM+SLQ LLN+R LGQVK+TT+V +TAL  QSPPSSGAFDP +  NM+GIL FLW
Sbjct: 129 DNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLW 188

Query: 187 EQGSPLMVSLYPHKTYFYSG---NMSLGYATF---AEENFSIR-DGDLSYNNLFDEMMDA 246
            QGSPLM+SLYP+  Y Y+G   N+SLGYATF    E+N  IR DGDLSYNN+FDEM+DA
Sbjct: 189 GQGSPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRTDGDLSYNNIFDEMVDA 248

Query: 247 FYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIY 306
           F+AAIDK  VGDVAIA+GETGWPT G+      S+A TYN NFKNHITSGKGTPMK NIY
Sbjct: 249 FHAAIDKACVGDVAIAIGETGWPTNGNYG-AGPSLALTYNLNFKNHITSGKGTPMKPNIY 308

Query: 307 IQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
           I+GFI+S+FNENE+PEGES+ YGMFHVDSTPIY  VF
Sbjct: 309 IEGFIRSLFNENEKPEGESQFYGMFHVDSTPIYPSVF 344

[Close]

BLAST of Bhi02G001514 vs. NCBI nr
Match: XP_022968751.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima])

HSP 1 Score: 468.0 bits (1203), Expect = 6.8e-128
Identity = 229/335 (68.36%), Postives = 269/335 (80.30%), Query Frame = 0


Query: 1   MAKLSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEP 60
           M K+SH+I +FM  +  IVGAYDVLLGAYY L G+NL  P  VVQLC+KYNI RVRL   
Sbjct: 1   MVKISHMIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEVS 60

Query: 61  NLTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKV 120
           N+ V+E+FRG  ID+S GVPN+++ NMATN T VEEWF  YV P++ D+ INYIVVGDK 
Sbjct: 61  NIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVGDKA 120

Query: 121 IPGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGI 180
           IPGLD  IL VM+SLQ LLNA   G+ KLTTLV   AL+  +PPSSGAF+P I  NMRGI
Sbjct: 121 IPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFNALAVTNPPSSGAFNPAIVENMRGI 180

Query: 181 LHFLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFY 240
           L FL  +GSPLMVS+Y +  Y Y GN SLGYAT +E+N  +RDG LSYNNLFDEM+DAFY
Sbjct: 181 LEFLRGEGSPLMVSVYTYDQYVYGGNRSLGYATLSEQNSLVRDGMLSYNNLFDEMVDAFY 240

Query: 241 AAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQ 300
           AAIDKE+VGDVAI +GETGWPTCG+ N TT  +AA YNRNFK+HI+SGKGTP K NIYI+
Sbjct: 241 AAIDKENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKENIYIE 300

Query: 301 GFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
           GFI+SIFNEN++PEGES+CYGMF+VDSTPIYSPVF
Sbjct: 301 GFIRSIFNENQKPEGESRCYGMFNVDSTPIYSPVF 335

[Close]

BLAST of Bhi02G001514 vs. ExPASy TrEMBL
Match: A0A5D3CFE1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001650 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.3e-129
Identity = 240/337 (71.22%), Postives = 281/337 (83.38%), Query Frame = 0


Query: 5   SHII-VIFMVFVSSI-VGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNL 64
           SH+I ++FMV +SSI VGAY++LLGAYY L  NNLP PW+V+QLC+KYNIRR+R+ EPNL
Sbjct: 6   SHVIALLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNL 65

Query: 65  TVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIP 124
            V+ESFRG  I+VSFGV N LI NMATNHTAVEEWF NY+APFI D TINYIVVGDK +P
Sbjct: 66  DVIESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVP 125

Query: 125 GLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILH 184
           GLD  IL VM+SLQ LLN R LGQVK+TTLV ++AL  ++PPSSGAFDP +  NMRGIL 
Sbjct: 126 GLDNSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENMRGILQ 185

Query: 185 FLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFA---EENFSIR-DGDLSYNNLFDEMMDA 244
           FLW QGSPLM+SL P++ Y Y+ N+SLGYATF    E+N  IR DG+LSYNNLFDE++DA
Sbjct: 186 FLWGQGSPLMLSLNPYEAYAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDA 245

Query: 245 FYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIY 304
           FYAAIDK  VG VAIA+GETGWPT G  +  +  +AATYN NFKNHI+SGKGTPMK NIY
Sbjct: 246 FYAAIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIY 305

Query: 305 IQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
           I+GFIKS+FNENE+PEGES+ YGMF+VDSTPIYSPVF
Sbjct: 306 IEGFIKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of Bhi02G001514 vs. ExPASy TrEMBL
Match: A0A5A7UQE4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001680 PE=3 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 3.0e-129
Identity = 241/337 (71.51%), Postives = 281/337 (83.38%), Query Frame = 0


Query: 5   SHII-VIFMVFVSSIV-GAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPNL 64
           SH+I ++FMV +SSIV GAY+VLLGAYY L  NNLP PW+V+QLC+KYNIRR+R+ EPNL
Sbjct: 6   SHVIALLFMVDMSSIVLGAYNVLLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNL 65

Query: 65  TVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVIP 124
            V+ESFRG  I+VSFGV N LI NMATNHTAVEEWF NY+APFI D TINYIVVGDK +P
Sbjct: 66  DVIESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVP 125

Query: 125 GLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGILH 184
           GLD  IL VM+SLQ LLN R LGQVK+TTLV ++AL  ++PPSSGAFDP +  NMRGIL 
Sbjct: 126 GLDDSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENMRGILQ 185

Query: 185 FLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFA---EENFSIR-DGDLSYNNLFDEMMDA 244
           FLW QGSPLM+SL P++ Y Y+ N+SLGYATF    E+N  IR DG+LSYNNLFDE++DA
Sbjct: 186 FLWGQGSPLMLSLNPYEAYAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDA 245

Query: 245 FYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIY 304
           FYAAIDK  VG VAIA+GETGWPT G  +  +  +AATYN NFKNHI+SGKGTPMK NIY
Sbjct: 246 FYAAIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIY 305

Query: 305 IQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
           I+GFIKS+FNENE+PEGES+ YGMF+VDSTPIYSPVF
Sbjct: 306 IEGFIKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of Bhi02G001514 vs. ExPASy TrEMBL
Match: A0A6J1HVR6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 3.3e-128
Identity = 229/335 (68.36%), Postives = 269/335 (80.30%), Query Frame = 0


Query: 1   MAKLSHIIVIFMVFVSSIVGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEP 60
           M K+SH+I +FM  +  IVGAYDVLLGAYY L G+NL  P  VVQLC+KYNI RVRL   
Sbjct: 1   MVKISHMIGLFMADMFLIVGAYDVLLGAYYGLLGDNLSSPASVVQLCRKYNISRVRLEVS 60

Query: 61  NLTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKV 120
           N+ V+E+FRG  ID+S GVPN+++ NMATN T VEEWF  YV P++ D+ INYIVVGDK 
Sbjct: 61  NIDVIEAFRGTEIDLSLGVPNDMLPNMATNKTLVEEWFNTYVEPYVNDYIINYIVVGDKA 120

Query: 121 IPGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGI 180
           IPGLD  IL VM+SLQ LLNA   G+ KLTTLV   AL+  +PPSSGAF+P I  NMRGI
Sbjct: 121 IPGLDDNILPVMKSLQDLLNAHYFGEAKLTTLVGFNALAVTNPPSSGAFNPAIVENMRGI 180

Query: 181 LHFLWEQGSPLMVSLYPHKTYFYSGNMSLGYATFAEENFSIRDGDLSYNNLFDEMMDAFY 240
           L FL  +GSPLMVS+Y +  Y Y GN SLGYAT +E+N  +RDG LSYNNLFDEM+DAFY
Sbjct: 181 LEFLRGEGSPLMVSVYTYDQYVYGGNRSLGYATLSEQNSLVRDGMLSYNNLFDEMVDAFY 240

Query: 241 AAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMKSNIYIQ 300
           AAIDKE+VGDVAI +GETGWPTCG+ N TT  +AA YNRNFK+HI+SGKGTP K NIYI+
Sbjct: 241 AAIDKENVGDVAIVIGETGWPTCGNYNATTPLLAAEYNRNFKSHISSGKGTPRKENIYIE 300

Query: 301 GFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
           GFI+SIFNEN++PEGES+CYGMF+VDSTPIYSPVF
Sbjct: 301 GFIRSIFNENQKPEGESRCYGMFNVDSTPIYSPVF 335

[Close]

BLAST of Bhi02G001514 vs. ExPASy TrEMBL
Match: A0A5A7UIV1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001690 PE=3 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.4e-126
Identity = 238/341 (69.79%), Postives = 276/341 (80.94%), Query Frame = 0


Query: 5   SHIIVI--FMVFVSSI-VGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPN 64
           SH+I +  FMV +SSI V AYDVLLGAYY   GNNLPPP +VVQLC+KYNIRR+R  EPN
Sbjct: 6   SHVIALLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPN 65

Query: 65  LTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVI 124
             ++++FRG  I++SF V   LI NMATNHTAVEEWF+NYVAPFIGDFTINYI+VGDK I
Sbjct: 66  FDIIDAFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAI 125

Query: 125 PGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGIL 184
           PGLD  IL VM+SLQ LLN R LGQVK+TTLV + AL  Q+PPSSG FDP +  NMRGIL
Sbjct: 126 PGLDDNILPVMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFDPNVLENMRGIL 185

Query: 185 HFLWEQGSPLMVSLYPHKTYFYSG---NMSLGYATF---AEENFSIRD-GDLSYNNLFDE 244
            FL  QGSPLM+SLYP++ Y Y+G   N+SLGYA F    E+N  IR  GDLSYNNLFDE
Sbjct: 186 QFLHGQGSPLMLSLYPYQEYAYTGNSNNISLGYANFNSQLEKNPPIRTYGDLSYNNLFDE 245

Query: 245 MMDAFYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMK 304
           M+D FYAAIDK +VGDV I +GETGWPT G+    + S+AATYNRNFKNHI+SGKGTPMK
Sbjct: 246 MVDTFYAAIDKANVGDVPIVIGETGWPTNGNYG-GSPSLAATYNRNFKNHISSGKGTPMK 305

Query: 305 SNIYIQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
            NIYI+GFI+S+FNENE+PEGES+ YGMF+VDSTPIYSPVF
Sbjct: 306 PNIYIEGFIRSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 345

[Close]

BLAST of Bhi02G001514 vs. ExPASy TrEMBL
Match: A0A5D3CDU1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001660 PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.5e-125
Identity = 237/341 (69.50%), Postives = 274/341 (80.35%), Query Frame = 0


Query: 5   SHIIVI--FMVFVSSI-VGAYDVLLGAYYRLAGNNLPPPWEVVQLCKKYNIRRVRLYEPN 64
           SH+I +  FMV +SSI V AYDVLLGAYY   GNNLPPP +VVQLC+KYNIRR+R  EPN
Sbjct: 6   SHVIALLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPN 65

Query: 65  LTVLESFRGMGIDVSFGVPNNLIENMATNHTAVEEWFINYVAPFIGDFTINYIVVGDKVI 124
             ++++FRG  I++SF V   LI NMATNHTAVEEWF+NYVAPFIGDFTINYI+VGDK I
Sbjct: 66  FDIIDAFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAI 125

Query: 125 PGLDGRILQVMQSLQGLLNARNLGQVKLTTLVSITALSAQSPPSSGAFDPTIAANMRGIL 184
           PGLD  IL VM+SLQ LLN R LGQVK+TTLV + AL  Q+PPSSG FDP +  NMRGIL
Sbjct: 126 PGLDDNILPVMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFDPNVLENMRGIL 185

Query: 185 HFLWEQGSPLMVSLYPHKTYFYSG---NMSLGYATF---AEENFSIRD-GDLSYNNLFDE 244
            FL  QGSPLM+SLYP++ Y Y+G   N+SLGYA F    E+N  IR  GDLSYNNLFDE
Sbjct: 186 QFLHGQGSPLMLSLYPYQEYAYTGNSNNISLGYANFNSQLEKNPPIRTYGDLSYNNLFDE 245

Query: 245 MMDAFYAAIDKEDVGDVAIAVGETGWPTCGHLNITTTSIAATYNRNFKNHITSGKGTPMK 304
           M+D FYAAI K +VGDV I +GETGWPT G+    + S+AATYNRNFKNHI+SGKGTPMK
Sbjct: 246 MVDTFYAAIGKANVGDVPIVIGETGWPTNGNYG-GSPSLAATYNRNFKNHISSGKGTPMK 305

Query: 305 SNIYIQGFIKSIFNENEEPEGESKCYGMFHVDSTPIYSPVF 336
            NIYI+GFI+S+FNENE+PEGES+ YGMF VDSTPIYSPVF
Sbjct: 306 PNIYIEGFIRSLFNENEKPEGESRFYGMFDVDSTPIYSPVF 345

The following BLAST results are available for this feature:

BLAST of Bhi02G001514 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20340.1	4.8e-63	40.84	beta-1,3-glucanase 5	[more]
AT5G20330.1	4.1e-62	39.68	beta-1,3-glucanase 4	[more]
AT5G20390.1	1.7e-60	38.02	Glycosyl hydrolase superfamily protein	[more]
AT5G20560.1	6.1e-58	38.03	Glycosyl hydrolase superfamily protein	[more]
AT1G33220.1	1.0e-57	38.67	Glycosyl hydrolase superfamily protein	[more]

BLAST of Bhi02G001514 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49353	6.8e-62	40.84	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49352	5.7e-61	39.68	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O22317	9.4e-56	37.58	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
P07979	9.1e-51	36.23	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
P12257	9.5e-48	34.85	Lichenase-2 (Fragment) OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]

BLAST of Bhi02G001514 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880685.1	4.3e-191	100.00	probable glucan endo-1,3-beta-glucosidase BG5 [Benincasa hispida]	[more]
TYK10032.1	4.7e-129	71.22	lichenase-2-like [Cucumis melo var. makuwa]	[more]
KAA0055781.1	6.2e-129	71.51	lichenase-2-like [Cucumis melo var. makuwa]	[more]
XP_031742611.1	5.2e-128	70.62	glucan endo-1,3-beta-glucosidase-like [Cucumis sativus] >XP_031742618.1 glucan e...	[more]
XP_022968751.1	6.8e-128	68.36	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita maxima]	[more]

BLAST of Bhi02G001514 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CFE1	2.3e-129	71.22	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7UQE4	3.0e-129	71.51	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1HVR6	3.3e-128	68.36	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111467895 PE=...	[more]
A0A5A7UIV1	1.4e-126	69.79	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3CDU1	1.5e-125	69.50	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 27..332 e-value: 3.7E-62 score: 210.7
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 251..264
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 25..335 e-value: 4.4E-89 score: 300.6
None	No IPR available	PANTHER	PTHR32227:SF305	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 19..332
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 19..332
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 27..332

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M001514	Bhi02M001514	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds