Bhi02G001421 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G001421
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	L-ascorbate oxidase
Location	chr2: 46265246 .. 46272338 (-)
RNA-Seq Expression	Bhi02G001421
Synteny	Bhi02G001421

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAATTTTCATTATAAAAAGGAGCCAATTGTAAATTGCAGTGGCATCTTTCACAAACTCCCCTTCTTCCCTCCTTACTCATTGCTTTGCTTATTGTTCTTCCCTGCCTGGAGAATAATAATATGGCAAAAGCTGCAAATCAGCCATTGTTTCCTCTGCCTTTTCTAGTCATCTTCTTGATTTTCTCAATGATTTCTGGGTTTGGAATAACTCTCTCTGAGGCAGTTAACAATACCAGATACTTCAAATGGGAGGTTGAGTACATGTTTTGGTCCCCGGACTGTGTCGAAAACCTCGTCATGGGAATCAATGGCCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGCGACACCATCATTGTAGAGCTGGTTAACAAGCTCTTCACTGAAGGTGTTGTTATTCATTGGCATGGAATCTTGCAGAGGGAAACTCCCTGGGCCGATGGCACTGCTTCCATCTCGCAATGTGCCATTAATCCAGGTGAAACCTTCATTTATCAGTTCAAAGCAGATAAGGTGAGAAAGTTTTAAAATGTCAATGTCGATGAAAGTGCAATTACGTTTCCGAAAAAAATTATTCATTTGTTGAATTTTTTTTTGCAGCCTGGGACATATTTCTATCATGGCCATTTGGGAATGCAGAGATCGGCAGGGTTGTATGGATCCTTAATAATAGATCCAGAAGAAGGAAAGAAAGAGCCTTTCCATTATGACGAAGAAATCAACTTATTGCTTAGTGATTGGTGGCATAAAAGTGCTCACAAGCAAGAAGTTGGCCTCAGCTCCAGCCCAATGCGTTGGATTGGTGAACCCGAGGTAGTTTAATTTATATATTATCTATTAAATTAATGTTTGGATTAATAATCCAATAGCATATTTCGTTAAATTATACAAAATATTTCTGAATTGTGAGATAGATTCAAATATTGTACTCTAACTCTGAAATTTTTCAATTTTATTCATGTATTTATTTTGAAATTTTATTTTAATATATATACCTCACTGAGAGTGTTTTTATTTTTTGTTTTTACTATTAAACAAAAAGATGGAGATGGCATGATGGTTAAGGTTTAAAAATTTAAAGGCCTACTTTAACCTCAAAAAAAAATTATGGAGTAATTTCAATATTTTAGGATAATTTTTTGAAACACAGTTTTAAAATTTATGCATAAAAATAAACACATCTCAAATCAAAAGAGATAAAATTGAAACATATGTGTGGTTTCTTAGTTAAATTCTTAAAATAAAATCAATTTATTTTTATTTTTTTTTAAAATTTTGACTTGTTTTTTCAAAACATTGCTTGGATGTAGATAATAAAAACCAATAAATTTATTGGTAAAAATAGTATTATAATTTTAATTTTAAGCAAAAAGCAAAAGTCCCGGTTAATAACCATTTGGTATTTTATTATTATTTTTTATAATTAAGTTGATTTCTTTTCAGTTTAATATTTCAAAAACAAAAATAGATTTTTGAAAATATAATACAAAGCAATAAATTTGGAAATAGGAGAGGTAGCCATTTCATTTTTGATTTTTGATTTTTGAAAACTAAAAAAAAAGCTCCATTTGGTAACCCTTTCATTTTTTTTTTTTTGTCTTTTTGATTTTTGAAAATTAATCTTATTTCTTCAAATTATATCCATTTTTAGATGTGGAAAATAAGTTTTCAGCCAAATTTTAAAAATAAAAACAAATTTTTAAAAATTGGCTTGGTTACGTTTTTGAAAATATATGTAAAAAAGGATTTTAAAAAACACAAAAAGATTAGAGGGTTATTGGCACATATAATTATTATATGTATTAAAATAAGTATATAATTCAAGGGTAAAATTTACACGAGTGTCAAAGAGAACTTGACTTAATGATATTGACATGATTTTCATTTCTAAAGATTGGAGGTTTAATCTCTCATCCTACATATTCAATACTAAAAAAGAAAATTACACAAGGATGAAACAATAAGAATCAAAACACATGGTTTTTTTTTTAAAGAAAATAACAAAAATAAAAATACACATATCTTTTTGAGACATGTATATAACTTAAGGTGTAATTTCCAACAAAAGAAAACATATTCAGAAAATATTCTCCCTCTTCTAAATTCCTTCCTTGCTTGAAATATGGTTTTTGTTATACATATAATAATTACTTTAATCAATGAAATTGTTGTACACCCTATGATTAGGGTCCTTGTGTACCAATGGATATCCATAGATTTCATTCCATAAATGAAATTGTTTCTTATAAAAAAAAATATATATATAATAATTACTTTTTATATTAGACAAATTATTTCGTTGACTTTTTTGGAAAGTTCAAATTAAGATTTTGTAATTTTTTTTTTTTTTAAACATTCTATAATTTTTCATAAAGTCATGAGATTAACTTATCTTTTATAAACATGTCAAAACTATTCTTAGAATAGAAATTCTGAAATATTTGATAAATAATAGAACTTAAATTGTGACCGGATATTGCCACACTTATCTAAGTTACCAATATACAAATGGAGTTAATATAGTCTCAATTTCCATCTAAAAATCTAAATGATTTCTATTTTAAAAATAGTTTTGAAATATTTATTAACGTAAAAATAATACTATACCTTTGAAAAGGATATAAGAGTATGTTTGAAATGAATGTTAATTTTTTTTTTTTTTCAATTTAGCCATATAATACATAAACAAAATTTGGATTATATGTAACCATGTAAGAAAGTAAAAAAATTGGAAATTAGACCACCCTACATAGGGTAAAGTTTGATTTTTTTGTTTTTTTAAGGAAAAAAAGTTTGTCATTAGTGTTTTAACTTGGTTGATTTAGGTAAAGTATTGATTAGAGATTTAGTAATTGTACTTTATCTTGTTGATTAATGGTATTTGAGAGGTTTAAATATAATCTTTTTTTTGTGTCCTTGTCTTTTCTTCTTGAAGACCATACTGATAAATGGGAGAGGGCAGATCAGTTGTTCAATTGCAGCTAAATACAACAAGAATTTGGAGCAATGTAAGTTGAAAGGAGGTGAACAATGTGCACCATTTATCCTCCAAGTCAAACCCAAGAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTAGCCATTGGGGTAACTCTCTCTCTCTTACTAAAGAAAAATCCAGATTGAACTTAATAATTATTCAAAGTGAACATAACTCAACCGATATACGAACGTGTTAGCAATCAAGAGACCTATAATTTGTATCTCCGGACCTTTAAATAAATAAATAATGTAAAACAACTATAGTCACTAGATGTATTTTGTAAACTTAATCTTTGAACTTTAAGGTTTATGTTTACTTGATCCCTCCGTTTTCTAAAGTTGTGTTTAATATATCCCTGAAATTTTTTAAATTTTAAAAAATTGATTGATCTATTAGATATAAATTTGAATTTTACATTTAATGAGGCTCTAAAGTTCAATTTTGAATTCTACTTGATATGTAAATTTTTAAAACACAAAAATTTAGGTTTTTTTTTTTTAAATTTTATTAAGCCATATATAGACTAACGGTATTTTGACGGTTATTTCCGATAATTTTTGTGCTTGACCTATAAAGTTCTATAAGAGAGGTAGATGTGAAACACAAAATTTTTAGTCTTTTAGAAATTTTTTCATAGTAACACTACCATGTCAATATGTTTTTAAAAATAATAAAAAGTCCATTAAAATTAAAAACTTGTTTATGAAGAAATTTTTTAGTGTGAATGAAAAAAATATAGTATTGACATAGTATTTGAAATTTTTTTATATAACTTTTTAATTTTTAGGCAATTTTCCATCTTTAACTCATGAAGCAATATTGAGAGAGTCTACACAGATTCCCCTTATGACAAAGGATGTGATTGACAACCCCACATGTTTTCCTCCAATTTTACTTTTTACTTGAATTTCAATATTTATTCTAAAAATATTATTGGGATGTTATCTTTTATTAAAACAAGTCGGAATCGCTAGTAATTGTCGGTCAGTCATACGGCGGGCAAATAATTCTTAATTGTTGATACCAAAGATAAAGCAGCGGATTCAGTAGCCCGGGCTGCAACAAGGACTCAGTGTCATTATGTTAATAAAAAATGGCTCATATAAAAATTTACTTAAGAACTAATATATAAAAATAATAAGTAATATGACATTATTTATTAGATAATTTATAAGACCGTTTAGGGTTCCAAATTGTAAGATTAAGTTTCTCCATAATTATAAAAAATAGATTAAAAAATAAGATTGAGTTTATTGGTAAATATAAAAAGTATTAAAAAATATGAAGATAGGACTACTCTATAAATATGTCACATTTATTAGTGAAATTAATTTGTTATACCAAGTCACCAACTTAAGAAGTTATGGACAAATGACTCCATATTATAATTCGTAAAATGAAGTTTAGGGAAGTAACTAGAAAGTATTTTCTAGTTAACAAAAATTTTAATAATTTAACTTTATTTTTTCTCTCTTCTTAATTAAAAAAAATGATTTATGAGATCTACATGGCCTTTCATGTGGATCCACCTTTCTTTATTTCTTTATTTTAGAAGGATCTACCTTTATTCTTCTTTAGGAAATAAAATTTAAATTTAAGGTAAAATTACCATTTTTTTATCATTTTATTTTTATCAATCTACTATAATGACTAATTAAATTAGCATTTTTAGAAATATCATTGTCAATAAATGTACTTAAGCCTCATTTTATAGAAATATCAACAAATAAAATTATGTCGATTGATATTTATGAAAAAAATTATAAAATCAGAAAAACAAAGAAAAAATATTCAAATTAGTAAATAGATATTTAAAATACAAATTAAAATGTTTACTATTTATATTATATGTACATGATTAATATTTCTTACTTATTTTTATGTATTTCATGAATTTTTTTACTATATAATAGAAATATTGAATCAAGCTTAATGAAAATTTTGGTTAGGTGGTGCATCTTTAACATTGTCTTCTATTATAATTAAATTCAGGTATTTTCATTATTGGGTTTGAACATTCACATGACTTTGATTTGATGATAAAATTTCTCTAATTTAGTTTTGAATAGCTATGAAATTCACATACATATATATATAAAATAAAATTAATAGCTTGAAGTAGCATATTAATTCATTTTAAAGTAGTAAGTTAATCTTTCTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATCCATTTTACATGTGTATTAAAAAATTAAAATATGATTTTAGTTCATATTTTGGAGTTAGTTTAATTTTAGTTCTTATACTTTTAAATATTTGATTTTAGAATCTATACTTTTAATAAATCTTAATTTTAATCCATACTCTTAATTAGTAATGTCCAATTTTTCATTACTCTTTATTTTTATTAATATTTTTGCTATAAATTTTCACAATGAATTTTTTTTTTTGTTGTTTAATCAATTTTGATAATAATTAATTTTGAGGGACTAAAATTAAACATTTGAAAATTAAAAAAATCAAAAATAAAAAATATAAGAAAAGCTATTTACATAAAATAGGAGTTTATTAGTGATAGAAAGAATTCTATCAGGTTGATAGAAGTGTTCTTTTTGCTATTTTTATAAATTGTTGACATTTTTTTTATCTATAAAAATTTTAAAAAAAAAAAAAAAAAAAAAACCTAAAACTAAACAAATTTAAGTTTTTTCTCCTATCATCTTTAGAATTATAAAAGTCGATAGAAGTTATGAGTGAGATTTTTATTTATTTATGTAGATAGTTTGACTTTTTTTATCAAGAAATTTTTTCTAAAAGAGAACTAAAATTAAACAAATTTAACTTTTTTTTTTTCATCTTCTTTAGAATCACCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTGAGCCATTTGTAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTCATCACCACCAACCAAGACCCAGCACAGAACTACTGGGTTTCCGTCGGCGTCCGTGGGCGGCTTCCCAAAACGCCGCCTGCACTGACTCTCCTCAATTACCTCCCGAACTCCGTCGCCAAATTACCCGTTTCTCCGCCGCCAGAAACCCCCAATTGGGAGGATTACGCCCGAAGCAAAAACTTCACATACAGAATCTTAGCCGCTATGGGCAGCCCAAACCCACCGATCAAATTTAACCGCCGCATCTTCCTTCTAAACACCCAAAATCGAATGAACGGCGGCTTCACAAAATGGGCAATCAACAACGTCTCTTTATCCCTCCCCTCAACGCCGTACCTTGGCGCCATCAAAATCGGGCTAAACAACACCTTCAACCAAAATCCCCCACCGGAAACATTCTCGGAGGATTACGACATCTTCAACCCACCGCCAAACCCGAACACGATCACCGACAACGGCGTTTACATGTTCAATTTGGGGGAAACGGTGGATGTGATTCTACAAAACGCTAACACATTGAACGACAAACATAGTGAGATTCACCCTTGGCATTTACACGGGCATGATTTTTGGGTTTTGGGGTATGGAGAGGGGAAATTTTCCGCCGCCGTGGATGAGAAGAAATTGAATCTGAAAAACCCACCGTTGAGGAACACGGTGGTGATATTCCCATATGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCAGGTGTTTGGGCGTTTCATTGTCACATTGAACCACATTTACATATGGGAATGGGGGTTATTTTCGCCGAAGGAGTTCATAAGGTTGGAACAATTCCGACGAAGGCTCTGGCTTGCGGCGGCACTGCGAAAGTGCTTATTAATAACCCTACCCAAAAACCCTAGAATGGGAATCCTAGAATACCCTTGAAAAACCACAGATATGTGGTTCTTTAGGGTTTATGGAAGTTTTCTTGTCTTATGTTTATGAGCACCTTTTTTTTTCTTAATAAAATTTGGCTGGTTGTATTTCGAAGTTTCTTTTCAAGTTTGATTCATGTACTTTTAAATATTCATAAATATAATCTCTATCGCTTATTTCATCTTTTTGTAAACTTTTTATCGTTTATTAAAATTTTTACTTCTCTCTATCATATTATATATTTTCTT

mRNA sequence

CAAATTTTCATTATAAAAAGGAGCCAATTGTAAATTGCAGTGGCATCTTTCACAAACTCCCCTTCTTCCCTCCTTACTCATTGCTTTGCTTATTGTTCTTCCCTGCCTGGAGAATAATAATATGGCAAAAGCTGCAAATCAGCCATTGTTTCCTCTGCCTTTTCTAGTCATCTTCTTGATTTTCTCAATGATTTCTGGGTTTGGAATAACTCTCTCTGAGGCAGTTAACAATACCAGATACTTCAAATGGGAGGTTGAGTACATGTTTTGGTCCCCGGACTGTGTCGAAAACCTCGTCATGGGAATCAATGGCCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGCGACACCATCATTGTAGAGCTGGTTAACAAGCTCTTCACTGAAGGTGTTGTTATTCATTGGCATGGAATCTTGCAGAGGGAAACTCCCTGGGCCGATGGCACTGCTTCCATCTCGCAATGTGCCATTAATCCAGGTGAAACCTTCATTTATCAGTTCAAAGCAGATAAGCCTGGGACATATTTCTATCATGGCCATTTGGGAATGCAGAGATCGGCAGGGTTGTATGGATCCTTAATAATAGATCCAGAAGAAGGAAAGAAAGAGCCTTTCCATTATGACGAAGAAATCAACTTATTGCTTAGTGATTGGTGGCATAAAAGTGCTCACAAGCAAGAAGTTGGCCTCAGCTCCAGCCCAATGCGTTGGATTGGTGAACCCGAGACCATACTGATAAATGGGAGAGGGCAGATCAGTTGTTCAATTGCAGCTAAATACAACAAGAATTTGGAGCAATGTAAGTTGAAAGGAGGTGAACAATGTGCACCATTTATCCTCCAAGTCAAACCCAAGAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTAGCCATTGGGAATCACCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTGAGCCATTTGTAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTCATCACCACCAACCAAGACCCAGCACAGAACTACTGGGTTTCCGTCGGCGTCCGTGGGCGGCTTCCCAAAACGCCGCCTGCACTGACTCTCCTCAATTACCTCCCGAACTCCGTCGCCAAATTACCCGTTTCTCCGCCGCCAGAAACCCCCAATTGGGAGGATTACGCCCGAAGCAAAAACTTCACATACAGAATCTTAGCCGCTATGGGCAGCCCAAACCCACCGATCAAATTTAACCGCCGCATCTTCCTTCTAAACACCCAAAATCGAATGAACGGCGGCTTCACAAAATGGGCAATCAACAACGTCTCTTTATCCCTCCCCTCAACGCCGTACCTTGGCGCCATCAAAATCGGGCTAAACAACACCTTCAACCAAAATCCCCCACCGGAAACATTCTCGGAGGATTACGACATCTTCAACCCACCGCCAAACCCGAACACGATCACCGACAACGGCGTTTACATGTTCAATTTGGGGGAAACGGTGGATGTGATTCTACAAAACGCTAACACATTGAACGACAAACATAGTGAGATTCACCCTTGGCATTTACACGGGCATGATTTTTGGGTTTTGGGGTATGGAGAGGGGAAATTTTCCGCCGCCGTGGATGAGAAGAAATTGAATCTGAAAAACCCACCGTTGAGGAACACGGTGGTGATATTCCCATATGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCAGGTGTTTGGGCGTTTCATTGTCACATTGAACCACATTTACATATGGGAATGGGGGTTATTTTCGCCGAAGGAGTTCATAAGGTTGGAACAATTCCGACGAAGGCTCTGGCTTGCGGCGGCACTGCGAAAGTGCTTATTAATAACCCTACCCAAAAACCCTAGAATGGGAATCCTAGAATACCCTTGAAAAACCACAGATATGTGGTTCTTTAGGGTTTATGGAAGTTTTCTTGTCTTATGTTTATGAGCACCTTTTTTTTTCTTAATAAAATTTGGCTGGTTGTATTTCGAAGTTTCTTTTCAAGTTTGATTCATGTACTTTTAAATATTCATAAATATAATCTCTATCGCTTATTTCATCTTTTTGTAAACTTTTTATCGTTTATTAAAATTTTTACTTCTCTCTATCATATTATATATTTTCTT

Coding sequence (CDS)

ATGGCAAAAGCTGCAAATCAGCCATTGTTTCCTCTGCCTTTTCTAGTCATCTTCTTGATTTTCTCAATGATTTCTGGGTTTGGAATAACTCTCTCTGAGGCAGTTAACAATACCAGATACTTCAAATGGGAGGTTGAGTACATGTTTTGGTCCCCGGACTGTGTCGAAAACCTCGTCATGGGAATCAATGGCCAGTTTCCCGGGCCAACGATTAGAGCCAACGTTGGCGACACCATCATTGTAGAGCTGGTTAACAAGCTCTTCACTGAAGGTGTTGTTATTCATTGGCATGGAATCTTGCAGAGGGAAACTCCCTGGGCCGATGGCACTGCTTCCATCTCGCAATGTGCCATTAATCCAGGTGAAACCTTCATTTATCAGTTCAAAGCAGATAAGCCTGGGACATATTTCTATCATGGCCATTTGGGAATGCAGAGATCGGCAGGGTTGTATGGATCCTTAATAATAGATCCAGAAGAAGGAAAGAAAGAGCCTTTCCATTATGACGAAGAAATCAACTTATTGCTTAGTGATTGGTGGCATAAAAGTGCTCACAAGCAAGAAGTTGGCCTCAGCTCCAGCCCAATGCGTTGGATTGGTGAACCCGAGACCATACTGATAAATGGGAGAGGGCAGATCAGTTGTTCAATTGCAGCTAAATACAACAAGAATTTGGAGCAATGTAAGTTGAAAGGAGGTGAACAATGTGCACCATTTATCCTCCAAGTCAAACCCAAGAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTGTCTGCCCTCAACTTAGCCATTGGGAATCACCAACTGTTAGTGGTAGAAGCCGACGGTAACTACGTTGAGCCATTTGTAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCCGTCCTCATCACCACCAACCAAGACCCAGCACAGAACTACTGGGTTTCCGTCGGCGTCCGTGGGCGGCTTCCCAAAACGCCGCCTGCACTGACTCTCCTCAATTACCTCCCGAACTCCGTCGCCAAATTACCCGTTTCTCCGCCGCCAGAAACCCCCAATTGGGAGGATTACGCCCGAAGCAAAAACTTCACATACAGAATCTTAGCCGCTATGGGCAGCCCAAACCCACCGATCAAATTTAACCGCCGCATCTTCCTTCTAAACACCCAAAATCGAATGAACGGCGGCTTCACAAAATGGGCAATCAACAACGTCTCTTTATCCCTCCCCTCAACGCCGTACCTTGGCGCCATCAAAATCGGGCTAAACAACACCTTCAACCAAAATCCCCCACCGGAAACATTCTCGGAGGATTACGACATCTTCAACCCACCGCCAAACCCGAACACGATCACCGACAACGGCGTTTACATGTTCAATTTGGGGGAAACGGTGGATGTGATTCTACAAAACGCTAACACATTGAACGACAAACATAGTGAGATTCACCCTTGGCATTTACACGGGCATGATTTTTGGGTTTTGGGGTATGGAGAGGGGAAATTTTCCGCCGCCGTGGATGAGAAGAAATTGAATCTGAAAAACCCACCGTTGAGGAACACGGTGGTGATATTCCCATATGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCAGGTGTTTGGGCGTTTCATTGTCACATTGAACCACATTTACATATGGGAATGGGGGTTATTTTCGCCGAAGGAGTTCATAAGGTTGGAACAATTCCGACGAAGGCTCTGGCTTGCGGCGGCACTGCGAAAGTGCTTATTAATAACCCTACCCAAAAACCCTAG

Protein sequence

MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP

Homology

[Close]

BLAST of Bhi02G001421 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-232
Identity = 364/557 (65.35%), Postives = 442/557 (79.35%), Query Frame = 0


Query: 30  TLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFT 89
           T S AV   R + WEVEY +WSPDC E  VM +NG+FPGPTI+A  GDTI+V L NKL T
Sbjct: 32  TASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTT 91

Query: 90  EGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAG 149
           EG+VIHWHGI Q  +PWADG A ++QCAINPGETF Y F  +KPGT+FYHGH GMQRSAG
Sbjct: 92  EGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAG 151

Query: 150 LYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILING 209
           LYGSLI+D  +GK E   YD E NLLLSDWWH++   QE+GLSS PMRWIGE ++ILING
Sbjct: 152 LYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILING 211

Query: 210 RGQISCSIAAKYNKN--LEQCKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIG 269
           RGQ +CS+AA+++ N  L  C  K G+QCAP IL V+P KTYRIR++STT+L++LNLA+ 
Sbjct: 212 RGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQ 271

Query: 270 NHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPA 329
            H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TT+QDP+QNY++SVGVRGR P T  A
Sbjct: 272 GHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQA 331

Query: 330 LTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLLNT 389
           LT+LNY+    +KLP SPPP TP W+D+ RSKNF+ +I +AMGSP+PP K+ +R+ LLNT
Sbjct: 332 LTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNT 391

Query: 390 QNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIFNPPPNPN 449
           QN ++ G+TKWAINNVSL  P+TPYLG++K  L   FN+  PP ++  DYDI NPPP PN
Sbjct: 392 QNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPN 451

Query: 450 TITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEK 509
           T T NG+Y+F    TVDVI+QNAN L    SEIHPWHLHGHDFWVLGYG+GKF   +DEK
Sbjct: 452 TTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEK 511

Query: 510 KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGT 569
             NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGV+FAEG++++G 
Sbjct: 512 TYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 571

Query: 570 IPTKALACGGTAKVLIN 585
           +P +AL CG T + L+N
Sbjct: 572 VPDEALGCGLTKQFLMN 584

[Close]

BLAST of Bhi02G001421 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 737.3 bits (1902), Expect = 9.9e-213
Identity = 349/575 (60.70%), Postives = 430/575 (74.78%), Query Frame = 0


Query: 15  LVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRAN 74
           +V +L+  ++  F    +  V +T    WEVEY +W PDC E +VM INGQFPGPTI A 
Sbjct: 4   IVWWLLTVVVVAFHSASAAVVEST----WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAV 63

Query: 75  VGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADKPG 134
            GDT+I+ +VNKL TEGVVIHWHGI Q+ TPWADG A ++QC INPGETF Y+F  DK G
Sbjct: 64  AGDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAG 123

Query: 135 TYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSS 194
           T+FYHGH GMQRS+GLYG LI+      KE   YD E NLLLSDWWH+S H QE+ LSS 
Sbjct: 124 THFYHGHYGMQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSR 183

Query: 195 PMRWIGEPETILINGRGQISCSIAAKYNKNLEQ--CKLKGGEQCAPFILQVKPKKTYRIR 254
           PMRWIGEP+++LINGRGQ +CS AA +NK  E+  C  K  +QCAP  L+V+P + YR+R
Sbjct: 184 PMRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLR 243

Query: 255 IASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYW 314
           IASTT+L++LNLA+  HQL+VVEADGNYV PF  +DID+YSGE+YSVL+ TN  P++ YW
Sbjct: 244 IASTTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYW 303

Query: 315 VSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSP 374
           +SVGVRGR PKTP ALT++NY+ ++    P  PPP TP W D  RSK+F+ +I AA G P
Sbjct: 304 ISVGVRGREPKTPQALTVINYV-DATESRPSHPPPVTPIWNDTDRSKSFSKKIFAAKGYP 363

Query: 375 NPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFN-QNPPPE 434
            PP K + ++ LLNTQN +   +TKW+INNVSLS+P TPYLG+I+ GL + ++ ++P  +
Sbjct: 364 KPPEKSHDQLILLNTQN-LYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKK 423

Query: 435 TFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFW 494
              ++YDI  PPPNPNT   +G+Y F  G  VDVILQNAN L    SEIHPWH+HGHDFW
Sbjct: 424 LIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFW 483

Query: 495 VLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLH 554
           VLGYGEGKF   +DEK  NLKNPPLRNTVV++P+GWTAIRFV DNPGVW FHCHIEPHLH
Sbjct: 484 VLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLH 543

Query: 555 MGMGVIFAEGVHKVG--TIPTKALACGGTAKVLIN 585
           MGMGV+F EGV ++G   IP +AL CG T K L+N
Sbjct: 544 MGMGVVFVEGVDRIGKMEIPDEALGCGLTRKWLMN 569

[Close]

BLAST of Bhi02G001421 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 731.9 bits (1888), Expect = 4.2e-211
Identity = 334/511 (65.36%), Postives = 403/511 (78.86%), Query Frame = 0


Query: 30  TLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFT 89
           T S AV   R + WEVEY +WSPDC E  VM +NG+FPGPTI+A  GDTI+V L NKL T
Sbjct: 32  TASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTT 91

Query: 90  EGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAG 149
           EG+VIHWHGI Q  +PWADG A ++QCAINPGETF Y F  +KPGT+FYHGH GMQRSAG
Sbjct: 92  EGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAG 151

Query: 150 LYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILING 209
           LYGSLI+D  +GK E   YD E NLLLSDWWH++   QE+GLSS PMRWIGE ++ILING
Sbjct: 152 LYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILING 211

Query: 210 RGQISCSIAAKYNKN--LEQCKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIG 269
           RGQ +CS+AA+++ N  L  C  K G+QCAP IL V+P KTYRIR++STT+L++LNLA+ 
Sbjct: 212 RGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQ 271

Query: 270 NHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPA 329
            H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TT+QDP+QNY++SVGVRGR P T  A
Sbjct: 272 GHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQA 331

Query: 330 LTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLLNT 389
           LT+LNY+    +KLP SPPP TP W+D+ RSKNF+ +I +AMGSP+PP K+ +R+ LLNT
Sbjct: 332 LTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRKRLILLNT 391

Query: 390 QNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIFNPPPNPN 449
           QN ++ G+TKWAINNVSL  P+TPYLG++K  L   FN+  PP ++  DYDI NPPP PN
Sbjct: 392 QNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPN 451

Query: 450 TITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEK 509
           T T NG+Y+F    TVDVI+QNAN L    SEIHPWHLHGHDFWVLGYG+GKF   +DEK
Sbjct: 452 TTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEK 511

Query: 510 KLNLKNPPLRNTVVIFPYGWTAIRFVADNPG 539
             NLKNPPLRNT +++PYGWTAIRFV DNPG
Sbjct: 512 TYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of Bhi02G001421 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.5e-165
Identity = 277/549 (50.46%), Postives = 361/549 (65.76%), Query Frame = 0


Query: 39  RYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHG 98
           R FKWEV+Y F SPDC E LV+ ING+FPGPTI+A  GDTI+VEL N   TE V +HWHG
Sbjct: 36  RRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHWHG 95

Query: 99  ILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDP 158
           I Q  TPW DG   ++QC I PGE FIYQF  D+PGTY YH H GMQR +GL G + + P
Sbjct: 96  IRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQVSP 155

Query: 159 EEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIA 218
              + EPF YD + N LL+DW+HKS  ++  GL+S P +W+GEP++++I GRG+ +CS  
Sbjct: 156 PATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASIPFKWVGEPQSLMIQGRGRFNCSNN 215

Query: 219 AKYNKNLEQ--CKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEA 278
                +L    C +   + C+ FIL V P KTYR+RI S T+LSAL+  I  H L VVEA
Sbjct: 216 LTTPPSLVSGVCNVSNAD-CSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVVEA 275

Query: 279 DGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPN 338
           DG+YVEPF   ++ +YSGE+YSVL+  +Q+P +NYW++  +  R   TPPA  +LNY PN
Sbjct: 276 DGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYYPN 335

Query: 339 SVAKLPVSPPPE--TPNWEDYARSKNFTYRILAAMGSPNP-PIKFNRRIFLLNTQNRMNG 398
              + P +       P W D       +  I A  G  +  P   ++ I LLNTQN +N 
Sbjct: 336 HPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQNEVN- 395

Query: 399 GFTKWAINNVSLSLPSTPYLGAIKIGLNNTFN-QNPPPETF-SEDYDIFNPPPNPNTITD 458
           G+ +W++NNVS   P TPYL A+K  L N F+ +   PE + S +YDIF  P N N  T 
Sbjct: 396 GYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNANATTS 455

Query: 459 NGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNL 518
           +G+Y      TVDVILQNANT+N  +SE HPWHLHGHDFWVLGYGEGKF+ + D K+ N 
Sbjct: 456 DGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPKRYNR 515

Query: 519 KNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTK 578
            +P  +NTV + P+GWTA+RF ADNPGVW+FHCHIE H  MGMG++F  G+ KV ++P+ 
Sbjct: 516 VDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSSLPSS 575

Query: 579 ALACGGTAK 581
            + CG T +
Sbjct: 576 IMGCGQTKR 582

[Close]

BLAST of Bhi02G001421 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 547.4 bits (1409), Expect = 1.5e-155
Identity = 248/384 (64.58%), Postives = 309/384 (80.47%), Query Frame = 0


Query: 203 ETILINGRGQISCSIAAKYNKN--LEQCKLKGGEQCAPFILQVKPKKTYRIRIASTTSLS 262
           ++ILINGRGQ +CS+AA+++ N  L  C  K G+QCAP IL V+P KTYRIR++STT+L+
Sbjct: 11  KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70

Query: 263 ALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGR 322
           +LNLA+  H+L+VVEADGNY+ PF T DIDIYSGESYSVL+TT+QDP+QNY++SVGVRGR
Sbjct: 71  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130

Query: 323 LPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNR 382
            P T  ALT+LNY+    +KLP SPPP TP W+D+ RSKNF+ +I +AMGSP+PP K+ +
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMGSPSPPKKYRK 190

Query: 383 RIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIF 442
           R+ LLNTQN ++ G+TKWAINNVSL  P+TPYLG++K  L   FN+  PP ++  DYDI 
Sbjct: 191 RLILLNTQNLID-GYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIM 250

Query: 443 NPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKF 502
           NPPP PNT T NG+Y+F    TVDVI+QNAN L    SEIHPWHLHGHDFWVLGYG+GKF
Sbjct: 251 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 310

Query: 503 SAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAE 562
              +DEK  NLKNPPLRNT +++PYGWTAIRFV DNPGVW FHCHIEPHLHMGMGV+FAE
Sbjct: 311 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 370

Query: 563 GVHKVGTIPTKALACGGTAKVLIN 585
           G++++G +P +AL CG T + L+N
Sbjct: 371 GLNRIGKVPDEALGCGLTKQFLMN 393

[Close]

BLAST of Bhi02G001421 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 1.5e-293
Identity = 468/582 (80.41%), Postives = 517/582 (88.83%), Query Frame = 0


Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFL  FL+ S+I GFGITLSEA     +++KW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGING+FPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP EG+ EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILING+GQ  CSIAAKYN+ L+QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V+PKKTYRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PFVTSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P +NYWVS+GVR RLPKTPP LTLLNYLPNS +KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K+ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           LN  FNQNPPPETF  +YDI NPPPNP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKV 582
           WAFHCHIEPHLHMGMGV+FAEGVH VG IP KALACG TA V
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALV 580

[Close]

BLAST of Bhi02G001421 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 961.8 bits (2485), Expect = 3.5e-279
Identity = 448/574 (78.05%), Postives = 492/574 (85.71%), Query Frame = 0


Query: 13  PFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIR 72
           P  +I   F +I  FGI  S   +  R++KWEVEYMFW+PDC EN+VMGINGQFPGPTIR
Sbjct: 10  PNFLILFFFGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIR 69

Query: 73  ANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADK 132
           AN GDT++VEL+NKL TEGVVIHWHGILQR TPWADGTASISQCAINPGETF Y F  D 
Sbjct: 70  ANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDN 129

Query: 133 PGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLS 192
           PGT+FYHGHLGMQRSAGLYGSLI+DP +GKKEPFHYD EINLLLSDWWH+S HKQEVGLS
Sbjct: 130 PGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS 189

Query: 193 SSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQVKPKKTYRIR 252
           S P+RWIGEP+TIL+NGRGQ  CSIAAKY+ NLE CKLKG E CAP+I  V PKKTYRIR
Sbjct: 190 SKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIR 249

Query: 253 IASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYW 312
           IASTT+L+ALN AIGNH LLVVEADGNYV+PF TSDIDIYSGESYSVLITT+Q+P++NYW
Sbjct: 250 IASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYW 309

Query: 313 VSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSP 372
           VSVG RGR P TPP LTLLNYLPNSV+KLP SPPPETP W+D+ RSKNFTYRI AAMGSP
Sbjct: 310 VSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMGSP 369

Query: 373 NPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPET 432
            PP+K NRRIFLLNTQN +N G+ KWAIN+VSL+LP TPYLGA+K  L + F+QNPPPE 
Sbjct: 370 KPPVKSNRRIFLLNTQNVIN-GYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEV 429

Query: 433 FSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWV 492
           F EDYDI  PP N  T   NGVY F +GE VDVILQNAN + +  SEIHPWHLHGHDFWV
Sbjct: 430 FPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWV 489

Query: 493 LGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM 552
           LGYG+GKF+A  +E  LNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM
Sbjct: 490 LGYGDGKFTAE-EESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHM 549

Query: 553 GMGVIFAEGVHKVGTIPTKALACGGTAKVLINNP 587
           GMGV+FAEGV KVG IPTKALACGGTAK LINNP
Sbjct: 550 GMGVVFAEGVEKVGRIPTKALACGGTAKSLINNP 579

[Close]

BLAST of Bhi02G001421 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 2.8e-276
Identity = 438/548 (79.93%), Postives = 480/548 (87.59%), Query Frame = 0


Query: 39  RYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHG 98
           R++KWEVEYMFW+P+C EN+VMGINGQFPGPTIRAN GD+++VEL NKL TEGVVIHWHG
Sbjct: 4   RHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWHG 63

Query: 99  ILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDP 158
           ILQR TPWADGTASISQCAINPGETF Y F  D PGT+FYHGHLGMQRSAGLYGSLI+DP
Sbjct: 64  ILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDP 123

Query: 159 EEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIA 218
            +GKKEPFHYD EINLLLSDWWH+S HKQEVGLSS P+RWIGEP+TIL+NGRGQ  CSIA
Sbjct: 124 PQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIA 183

Query: 219 AKYNKNLEQCKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADG 278
           AKY+ NLE CKLKG E CAP+I  V PKKTYRIRIASTT+L+ALN AIGNHQLLVVEADG
Sbjct: 184 AKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADG 243

Query: 279 NYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSV 338
           NYV+PF TSDIDIYSGESYSVLITT+Q+P++NYWVSVG R R P TPP LTLLNYLPNSV
Sbjct: 244 NYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSV 303

Query: 339 AKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKW 398
           +KLP SPPP+TP W+D+ RSKNFTYRI AAMGSP PP+KFNRRIFLLNTQN +N G+ KW
Sbjct: 304 SKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVKFNRRIFLLNTQNVIN-GYVKW 363

Query: 399 AINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFN 458
           AIN+VSL+LP TPYLGA+K  L + F+QNPPPE F EDYDI  PP N  T   NGVY F 
Sbjct: 364 AINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFK 423

Query: 459 LGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRN 518
           +GE VDVILQNAN + +  SE HPWHLHGHDFWVLGYG+GKFSA  +E  LNLKNPPLRN
Sbjct: 424 IGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAE-EESSLNLKNPPLRN 483

Query: 519 TVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGT 578
           TVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGV+FAEGV KVG IPTKALACGGT
Sbjct: 484 TVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGT 543

Query: 579 AKVLINNP 587
           AK LINNP
Sbjct: 544 AKSLINNP 549

[Close]

BLAST of Bhi02G001421 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 2.7e-239
Identity = 387/575 (67.30%), Postives = 459/575 (79.83%), Query Frame = 0


Query: 12  LPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTI 71
           L FL  FL+  ++     + S     TR+FKW+VEY+ WSPD  E++VMGINGQFPGPTI
Sbjct: 4   LGFLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTI 63

Query: 72  RANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKAD 131
           RA  GDT+ V L NKL TEGVVIHWHGI Q  TPWADGTA+ISQCAINPGETF+Y+FK D
Sbjct: 64  RAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVD 123

Query: 132 KPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGL 191
           K GTYFYHGH GMQRSAGLYGSLI++  EG+KEPFHYD E NLLLSDWWHK +H+QEV L
Sbjct: 124 KAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDL 183

Query: 192 SSSPMRWIGEPETILINGRGQISCSIAAKYNK-NLEQCKLKGGEQCAPFILQVKPKKTYR 251
           SS+P+RWIGEP+T+L+NGRGQ +CS+AA+++K  L QCKL+GGEQ AP IL+V+P K YR
Sbjct: 184 SSNPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYR 243

Query: 252 IRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQN 311
           +R+ASTT+L +L+LAIG H+++VVEADGNYV+PF   D+DIYSGESYSVL  T+QDP +N
Sbjct: 244 LRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKN 303

Query: 312 YWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMG 371
           YW+S+ VRGR PKTP  LTLLNYLPNS +K P  PPP  P W DY  SK+F+ +I A MG
Sbjct: 304 YWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMG 363

Query: 372 SPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPP 431
           SP PP + +RRI LLNTQN+++ G+TKWAINNVSL LP+  YLG+I+ G+ N F+  PPP
Sbjct: 364 SPKPPPQNHRRIILLNTQNKID-GYTKWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPP 423

Query: 432 ETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDF 491
           + F +DYD+    PN N+   NGVYM     T+D+ILQNAN L    SEIHPWHLHGHDF
Sbjct: 424 DNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDF 483

Query: 492 WVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHL 551
           WVLGYGEGKFS   D KK NLKNPPLRNT VIFP+GWTA+RFV DNPGVWAFHCHIEPHL
Sbjct: 484 WVLGYGEGKFSEK-DVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHL 543

Query: 552 HMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINN 586
           HMGMGVIFAEGVH V  IP +ALACG T K+L++N
Sbjct: 544 HMGMGVIFAEGVHLVKKIPKEALACGLTGKMLMSN 575

[Close]

BLAST of Bhi02G001421 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 5.3e-227
Identity = 363/556 (65.29%), Postives = 434/556 (78.06%), Query Frame = 0


Query: 30  TLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGINGQFPGPTIRANVGDTIIVELVNKLFT 89
           T S AV   R + WEVEY F  PDC E +VM +NGQFPGPTI A  GDTI+V L NKL T
Sbjct: 15  TASAAV---REYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLAT 74

Query: 90  EGVVIHWHGILQRETPWADGTASISQCAINPGETFIYQFKADKPGTYFYHGHLGMQRSAG 149
           EG+VIHWHGI Q  +PWADG A ++QCAI+PGETF Y F  DKPGT+FYHGH GMQRSAG
Sbjct: 75  EGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAG 134

Query: 150 LYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHKSAHKQEVGLSSSPMRWIGEPETILING 209
           LYGSLIID  +GKKEP  YD E NLLLSDWWH+    QE+GLSS PMRWIGE ++ILING
Sbjct: 135 LYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQSILING 194

Query: 210 RGQISCSIAAKYNK-NLEQCKLKGGEQCAPFILQVKPKKTYRIRIASTTSLSALNLAIGN 269
           RGQ +CS+AA+++  +L  C  K G+QCAP  L V+P KTYRIR+AS+T+L++LN A+  
Sbjct: 195 RGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQG 254

Query: 270 HQLLVVEADGNYVEPFVTSDIDIYSGESYSVLITTNQDPAQNYWVSVGVRGRLPKTPPAL 329
           H+L+VVEADGNY+ PF T DIDIYSGE+YSVL+TT+QDP+QNY+++ GVRGR P TPPAL
Sbjct: 255 HKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPAL 314

Query: 330 TLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFTYRILAAMGSPNPPIKFNRRIFLLNTQ 389
           T+LNY+    ++LP SPPPETP W D+ RSKNF+ +I AAMGSP+PP  F+ R+ LLNTQ
Sbjct: 315 TVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMGSPSPPETFDERLILLNTQ 374

Query: 390 NRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNNTFNQNPPPETFSEDYDIFNPPPNPNT 449
           N +  GFTKWAINNVSL++P TPYLG++K  L   FN++ PP+ +  DYDI  PP N N 
Sbjct: 375 NLIE-GFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMTPPRNRNA 434

Query: 450 ITDNGVYMFNLGETVDVILQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDEKK 509
              N   +F    TVDVILQNAN LN   SEIHPWHLHGHDFWVLGYGEGKF   VDEK 
Sbjct: 435 KQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDEKT 494

Query: 510 LNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVIFAEGVHKVGTI 569
            NLKNPPLRNTV ++PYGWTA+RFV DNPGVW FHCHIEPHLHMGMGV+FAEG++++G +
Sbjct: 495 YNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKV 554

Query: 570 PTKALACGGTAKVLIN 585
           P +AL CG T + L+N
Sbjct: 555 PDEALGCGLTKQFLMN 566

[Close]

BLAST of Bhi02G001421 vs. NCBI nr
Match: XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])

HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0


Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60
           MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM
Sbjct: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60

Query: 61  GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120
           GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP
Sbjct: 61  GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120

Query: 121 GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW 180
           GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW
Sbjct: 121 GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW 180

Query: 181 HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI 240
           HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI
Sbjct: 181 HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI 240

Query: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 300
           LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 300

Query: 301 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 360
           ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 360

Query: 361 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 420
           FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL
Sbjct: 361 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 420

Query: 421 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 480
           NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI
Sbjct: 421 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
           HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540

Query: 541 AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 590

[Close]

BLAST of Bhi02G001421 vs. NCBI nr
Match: NP_001284440.1 (L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucumis melo])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.8e-307
Identity = 492/588 (83.67%), Postives = 535/588 (90.99%), Query Frame = 0


Query: 3   KAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGI 62
           K ANQPLFP+PF  IF IFS+  GFGITLS+A + TR+FKWEVEYMF SPDC ENLVMGI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGE 122
           N QFPGPTIRANVGDT++VEL+NKL TE VVIHWHGILQ+ TPWADGTASISQCAINPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHK 182
           +F YQF  DKPGTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPFHYDEEINLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQ 242
           S  KQEVGLSSSPMRWIGEP++ILINGRGQ +CSI AKY KN+EQCKL G E+CAPFIL+
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLIT 302
           VKP KTYRIRI STTSLSALN AIGNH+LLVVEADGNYV+PF+TSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFT 362
           T+Q+P+QNYWVS+GVRGR PKTPP LTLLNYLPNS +KLP+SPPPETP W DY RSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNN 422
           YRILAAMGSP PP+K  RRIFLLNTQN+MNGG+TKWAINNVSL+LPSTPYLGAIKIG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHP 482
           TF+QNPPPET+ E+YDI  PPPN NTIT NGVY F LGE VDV+LQNANTLNDKHSEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVWAF
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           HCHIEPHLHMGMGV+FAEGV KVG IPT A+ACGGTAK LI NPTQKP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

[Close]

BLAST of Bhi02G001421 vs. NCBI nr
Match: TYK09892.1 (L-ascorbate oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.4e-306
Identity = 493/588 (83.84%), Postives = 534/588 (90.82%), Query Frame = 0


Query: 3   KAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGI 62
           K ANQPLFP+PF  IF IFS+  GFGITLS+A + TR+FKWEVEYMF SPDC ENLVMGI
Sbjct: 4   KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGE 122
           N QFPGPTIRANVGDTI+VEL+NKL TE VVIHWHGILQ+ TPWADGTASISQCAINPGE
Sbjct: 64  NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHK 182
           +F YQF  DKPGTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPFHYDEEINLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQ 242
           S  KQEVGLSSSPMRWIGEP++ILINGRGQ +CSI AKY KN+EQCKL G E CAPFIL+
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243

Query: 243 VKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLIT 302
           VKP KTYRIRI STTSLSALN AIGNH+LLVVEADGNYV+PF+TSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFT 362
           T+Q+P+QNYWVS+GVRGR PKTPP LTLLNYLPNS +KLP+SPPPETP W DY RSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNN 422
           YRILAAMGSP PP+K  RRIFLLNTQN+MNGG+TKWAINNVSL+LPSTPYLGAIKIG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHP 482
           TF+QNPPPET+ E+YDI  PPPN NTIT NGVY F LGE VDV+LQNANTLNDKHSEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVWAF
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           HCHIEPHLHMGMGV+FAEGV KVG IPT A+ACGGTAK LI NPTQKP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

[Close]

BLAST of Bhi02G001421 vs. NCBI nr
Match: XP_038878978.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1042.3 bits (2694), Expect = 1.5e-300
Identity = 489/592 (82.60%), Postives = 531/592 (89.70%), Query Frame = 0


Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++ LFP PFL IF I S+I GFGITLSEA     R++KWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKSLFPKPFL-IFFILSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGI+GQFPGPTIRANVGDT++VEL NKL TEGVVIHWHGI+QR TPWADGTASISQCAIN
Sbjct: 61  MGIDGQFPGPTIRANVGDTVVVELTNKLHTEGVVIHWHGIMQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DPEEGK EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPEEGKNEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS P+RWIGEP+TILINGRGQ  CSIAAKYN NL+QC+LKGGEQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPIRWIGEPQTILINGRGQFDCSIAAKYNNNLKQCELKGGEQCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V P KTYRIRIASTT+L++LN AIGNH+LLVVEADGNY++PF+TSDIDIYSGESYSV
Sbjct: 241 ILHVLPNKTYRIRIASTTALASLNFAIGNHRLLVVEADGNYIQPFLTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q  ++NYWVS+GVR RLPKTPP LT+LNYLPNSV+KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQPSSENYWVSIGVRARLPKTPPGLTILNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRRIFLLNTQNRMN GF KWAINNVSL+LP TPYL A+K  
Sbjct: 361 NFTFRIFAAMGSPKPPMRYNRRIFLLNTQNRMN-GFVKWAINNVSLALPPTPYLAAMKFK 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           LN  F+QNPPPETF  DYDI NPP N NTIT NGVY FN+GE VDVILQNAN LND  SE
Sbjct: 421 LNTAFDQNPPPETFPIDYDINNPPQNANTITGNGVYRFNMGEIVDVILQNANMLNDNTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLI-NNPTQKP 591
           WAFHCHIEPHLHMGMGV+FAEGVHKVG IPTKALACGG AK LI NNP Q P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHKVGRIPTKALACGGAAKALINNNPPQDP 590

[Close]

BLAST of Bhi02G001421 vs. NCBI nr
Match: XP_038880028.1 (L-ascorbate oxidase-like [Benincasa hispida])

HSP 1 Score: 1031.2 bits (2665), Expect = 3.6e-297
Identity = 486/590 (82.37%), Postives = 517/590 (87.63%), Query Frame = 0


Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60
           MA+ AN+PLFP+PF VIFL+ S+ISGFGIT S+A +  R   WE++YMFWSPDCVEN++M
Sbjct: 1   MAEVANRPLFPMPFPVIFLVLSIISGFGITPSQAASQIRNHTWEIDYMFWSPDCVENIIM 60

Query: 61  GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120
           GINGQFPGPTIRANV DTIIV + NKL TEGVVIHWHGILQR TPWADGTASISQCAINP
Sbjct: 61  GINGQFPGPTIRANVSDTIIVHVNNKLSTEGVVIHWHGILQRGTPWADGTASISQCAINP 120

Query: 121 GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW 180
           GE F YQF  DK GTYFYHGHLGMQRSAGLYGSLI+D EEGKKEPF YDEEINLLLSDWW
Sbjct: 121 GENFTYQFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDLEEGKKEPFDYDEEINLLLSDWW 180

Query: 181 HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI 240
           HKS HKQEVGL+S+P RWIGEPE+ILINGRGQ +CSIAAKYN NLEQC+LK  E+CAPFI
Sbjct: 181 HKSVHKQEVGLNSNPFRWIGEPESILINGRGQFNCSIAAKYNNNLEQCELKENEECAPFI 240

Query: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 300
           LQVKPKKTYRIRIASTTSLSALNLAIGNH LLVVEADGNYV PFVT DIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHSLLVVEADGNYVAPFVTYDIDIYSGESYSVL 300

Query: 301 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 360
           ITTNQDPAQNYWVSVGVRGRLP+T PALTLLNYLPNSV KLP+SPPPETP W+D+ARSK 
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPETAPALTLLNYLPNSVDKLPISPPPETPKWDDFARSKR 360

Query: 361 FTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGL 420
           FT RILA    P PP+ FNRRIFLLNTQN M GG  KWAINNVSL LPSTPYLGAIKIGL
Sbjct: 361 FTGRILATEDIPKPPVNFNRRIFLLNTQNLMKGGSIKWAINNVSLVLPSTPYLGAIKIGL 420

Query: 421 NNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEI 480
             TF+QNPPPETF EDYDI  PPPNPNT T NGVY F LGE VDVILQNAN L +  S+I
Sbjct: 421 KTTFDQNPPPETFPEDYDINKPPPNPNTTTGNGVYKFELGEVVDVILQNANMLKENTSDI 480

Query: 481 HPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 540
           HPWHLHGHDFWVLGYGEGKFSA VDE  LNL+NPPL+NTVVIFP GWTAIRFVADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSADVDENNLNLENPPLKNTVVIFPRGWTAIRFVADNPGVW 540

Query: 541 AFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           AFHCHIEPHLHMGMGVIFAE VH+VGTIPT ALACGG A+ LI  P  KP
Sbjct: 541 AFHCHIEPHLHMGMGVIFAEEVHRVGTIPTHALACGGIARTLIYRPATKP 590

[Close]

BLAST of Bhi02G001421 vs. ExPASy TrEMBL
Match: Q9M5B5 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 1.8e-307
Identity = 492/588 (83.67%), Postives = 535/588 (90.99%), Query Frame = 0


Query: 3   KAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGI 62
           K ANQPLFP+PF  IF IFS+  GFGITLS+A + TR+FKWEVEYMF SPDC ENLVMGI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGE 122
           N QFPGPTIRANVGDT++VEL+NKL TE VVIHWHGILQ+ TPWADGTASISQCAINPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHK 182
           +F YQF  DKPGTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPFHYDEEINLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQ 242
           S  KQEVGLSSSPMRWIGEP++ILINGRGQ +CSI AKY KN+EQCKL G E+CAPFIL+
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLIT 302
           VKP KTYRIRI STTSLSALN AIGNH+LLVVEADGNYV+PF+TSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFT 362
           T+Q+P+QNYWVS+GVRGR PKTPP LTLLNYLPNS +KLP+SPPPETP W DY RSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNN 422
           YRILAAMGSP PP+K  RRIFLLNTQN+MNGG+TKWAINNVSL+LPSTPYLGAIKIG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHP 482
           TF+QNPPPET+ E+YDI  PPPN NTIT NGVY F LGE VDV+LQNANTLNDKHSEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVWAF
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           HCHIEPHLHMGMGV+FAEGV KVG IPT A+ACGGTAK LI NPTQKP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

[Close]

BLAST of Bhi02G001421 vs. ExPASy TrEMBL
Match: A0A5D3CF11 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00020 PE=3 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 7.0e-307
Identity = 493/588 (83.84%), Postives = 534/588 (90.82%), Query Frame = 0


Query: 3   KAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVMGI 62
           K ANQPLFP+PF  IF IFS+  GFGITLS+A + TR+FKWEVEYMF SPDC ENLVMGI
Sbjct: 4   KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINPGE 122
           N QFPGPTIRANVGDTI+VEL+NKL TE VVIHWHGILQ+ TPWADGTASISQCAINPGE
Sbjct: 64  NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 TFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWWHK 182
           +F YQF  DKPGTYFYHGHLGMQR+AGLYGSLI+D EEGKKEPFHYDEEINLLLSDWWH+
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFILQ 242
           S  KQEVGLSSSPMRWIGEP++ILINGRGQ +CSI AKY KN+EQCKL G E CAPFIL+
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243

Query: 243 VKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVLIT 302
           VKP KTYRIRI STTSLSALN AIGNH+LLVVEADGNYV+PF+TSDIDIYSGESYSVLI 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKNFT 362
           T+Q+P+QNYWVS+GVRGR PKTPP LTLLNYLPNS +KLP+SPPPETP W DY RSKNFT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 YRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIGLNN 422
           YRILAAMGSP PP+K  RRIFLLNTQN+MNGG+TKWAINNVSL+LPSTPYLGAIKIG+N+
Sbjct: 364 YRILAAMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIND 423

Query: 423 TFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSEIHP 482
           TF+QNPPPET+ E+YDI  PPPN NTIT NGVY F LGE VDV+LQNANTLNDKHSEIHP
Sbjct: 424 TFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIHP 483

Query: 483 WHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAF 542
           WHLHGHDFWVLGYGEGKFSAAVDE KLNLK+PPLRNTVVIFP+GWT +RFVADNPGVWAF
Sbjct: 484 WHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWAF 543

Query: 543 HCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           HCHIEPHLHMGMGV+FAEGV KVG IPT A+ACGGTAK LI NPTQKP
Sbjct: 544 HCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTAKALIQNPTQKP 591

[Close]

BLAST of Bhi02G001421 vs. ExPASy TrEMBL
Match: A0A5A7UMY3 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G00010 PE=3 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 3.1e-291
Identity = 473/591 (80.03%), Postives = 519/591 (87.82%), Query Frame = 0


Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFLV F + S+I GFGITLSEA     R++KWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGINGQFPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP+EG  EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILINGRGQ  CSIAAKY   L+QC+L G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V PKKTYRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKTYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P++NYWVS+GVR RLPKTPP LT+LNYLPNSV+KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           L+  FNQNPPPETF  DYDI NPP NP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           WAFHCHIEPHLHMGMGVIFAEGVHKVG IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

[Close]

BLAST of Bhi02G001421 vs. ExPASy TrEMBL
Match: A0A0A0LXB9 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 5.4e-291
Identity = 468/582 (80.41%), Postives = 517/582 (88.83%), Query Frame = 0


Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFL  FL+ S+I GFGITLSEA     +++KW+VEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFL-SFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGING+FPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP EG+ EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILING+GQ  CSIAAKYN+ L+QC+L G E+CAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V+PKKTYRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PFVTSDIDIYSGESYSV
Sbjct: 241 ILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P +NYWVS+GVR RLPKTPP LTLLNYLPNS +KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K+ 
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           LN  FNQNPPPETF  +YDI NPPPNP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKF A  DEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKV 582
           WAFHCHIEPHLHMGMGV+FAEGVH VG IP KALACG TA V
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALV 580

[Close]

BLAST of Bhi02G001421 vs. ExPASy TrEMBL
Match: Q9M5B4 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 1.6e-290
Identity = 472/591 (79.86%), Postives = 518/591 (87.65%), Query Frame = 0


Query: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEA-VNNTRYFKWEVEYMFWSPDCVENLV 60
           MAK A++P FP PFLV F + S+I GFGITLSEA     R++KWEVEYMFWSPDCVEN+V
Sbjct: 1   MAKVADKPFFPKPFLV-FFVLSIIFGFGITLSEAGFPKIRHYKWEVEYMFWSPDCVENIV 60

Query: 61  MGINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAIN 120
           MGINGQFPGPTIRAN GD ++VEL NKL TEGVVIHWHGILQR TPWADGTASISQCAIN
Sbjct: 61  MGINGQFPGPTIRANAGDMVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAIN 120

Query: 121 PGETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDW 180
           PGETF Y+F  DK GTYFYHGHLGMQRSAGLYGSLI+DP+EG  EPFHYDEEINLLLSDW
Sbjct: 121 PGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPQEGTSEPFHYDEEINLLLSDW 180

Query: 181 WHKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPF 240
           WH+S HKQEVGLSS PMRWIGEP++ILINGRGQ  CSIAAKY   L+QC+L G EQCAPF
Sbjct: 181 WHQSVHKQEVGLSSKPMRWIGEPQSILINGRGQFDCSIAAKYKNGLKQCELSGKEQCAPF 240

Query: 241 ILQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSV 300
           IL V PKK YRIRIASTT+L++LN AIGNH+LLVVEADGNYV+PF TSDIDIYSGESYSV
Sbjct: 241 ILHVHPKKIYRIRIASTTALASLNFAIGNHKLLVVEADGNYVQPFSTSDIDIYSGESYSV 300

Query: 301 LITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSK 360
           LITT+Q+P++NYWVS+GVR RLPKTPP LT+LNYLPNSV+KLP+SPPPETP+WED+ RSK
Sbjct: 301 LITTDQNPSENYWVSIGVRARLPKTPPGLTVLNYLPNSVSKLPISPPPETPDWEDFDRSK 360

Query: 361 NFTYRILAAMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420
           NFT+RI AAMGSP PP+++NRR+FLLNTQNR+N GF KWAINNVSL+LP TPYL A+K  
Sbjct: 361 NFTFRIFAAMGSPKPPVRYNRRLFLLNTQNRIN-GFMKWAINNVSLALPPTPYLAAMKFR 420

Query: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480
           L+  FNQNPPPETF  DYDI NPP NP T T NGVY FN+GETVDVILQNAN LN   SE
Sbjct: 421 LSTAFNQNPPPETFPIDYDINNPPRNPETATGNGVYKFNMGETVDVILQNANMLNANTSE 480

Query: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLGYGEGKFSAA D +KLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAEDGRKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTAKVLINNPTQKP 591
           WAFHCHIEPHLHMGMGVIFAEGVHKVG IP  A+ACG TA  +I N  +KP
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGMIPPNAMACGSTA--MIKNYPRKP 587

The following BLAST results are available for this feature:

BLAST of Bhi02G001421 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G21105.1	1.1e-232	65.35	Plant L-ascorbate oxidase	[more]
AT5G21100.1	9.9e-213	60.70	Plant L-ascorbate oxidase	[more]
AT5G21105.3	4.2e-211	65.36	Plant L-ascorbate oxidase	[more]
AT4G39830.1	1.5e-165	50.46	Cupredoxin superfamily protein	[more]
AT5G21105.2	1.5e-155	64.58	Plant L-ascorbate oxidase	[more]

BLAST of Bhi02G001421 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14133	1.5e-293	80.41	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P24792	3.5e-279	78.05	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	2.8e-276	79.93	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
Q40588	2.7e-239	67.30	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]
M4DUF2	5.3e-227	65.29	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]

BLAST of Bhi02G001421 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880052.1	0.0e+00	100.00	L-ascorbate oxidase-like [Benincasa hispida]	[more]
NP_001284440.1	3.8e-307	83.67	L-ascorbate oxidase-like [Cucumis melo] >AAF35910.1 ascorbate oxidase AO1 [Cucum...	[more]
TYK09892.1	1.4e-306	83.84	L-ascorbate oxidase-like [Cucumis melo var. makuwa]	[more]
XP_038878978.1	1.5e-300	82.60	L-ascorbate oxidase [Benincasa hispida]	[more]
XP_038880028.1	3.6e-297	82.37	L-ascorbate oxidase-like [Benincasa hispida]	[more]

BLAST of Bhi02G001421 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q9M5B5	1.8e-307	83.67	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1	[more]
A0A5D3CF11	7.0e-307	83.84	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G0...	[more]
A0A5A7UMY3	3.1e-291	80.03	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G...	[more]
A0A0A0LXB9	5.4e-291	80.41	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G604040 PE=3 SV=1	[more]
Q9M5B4	1.6e-290	79.86	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492479 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017760	L-ascorbate oxidase, plants	TIGRFAM	TIGR03388	TIGR03388	coord: 39..578 e-value: 2.8E-297 score: 984.6
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 172..334 e-value: 5.0E-46 score: 156.7
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 440..561 e-value: 9.6E-36 score: 122.5
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 29..158 e-value: 3.3E-47 score: 162.2
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 166..371 e-value: 7.0E-65 score: 220.9
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 372..588 e-value: 7.5E-72 score: 243.4
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 165..370
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 374..577
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 38..183
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 45..160 e-value: 2.0E-40 score: 137.5
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 32..579
None	No IPR available	PANTHER	PTHR11709:SF394	PLANT L-ASCORBATE OXIDASE	coord: 32..579
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 538..558
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 543..554
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 378..569 e-value: 3.06096E-81 score: 250.03
IPR034259	Ascorbate oxidase, first cupredoxin domain	CDD	cd13845	CuRO_1_AAO	coord: 39..158 e-value: 4.25392E-81 score: 248.515

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M001421	Bhi02M001421	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity
molecular_function	GO:0016491	oxidoreductase activity