Bhi02G000585 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G000585
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	nicotianamine synthase-like
Location	chr2: 15630789 .. 15631871 (+)
RNA-Seq Expression	Bhi02G000585
Synteny	Bhi02G000585

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAAAAAACCAAGAGAAAAGAATACAAAAATTATCTTAACACTTCCCTAGCTTCCATTCATTTCCAATTATGGCAGCCCTCCAAATCCATGGCACCAAAACTGAAATCTCCACGGAGTTTCTAGTAGATCGAATCATCGATCTTCACGAAAGCATCTCGAAACTGGAAACCTTGAAACCATGCAAGAAAGTGAACAACCTCTTTACAAACCTAGTGAACTTGTGCATTTTGCCTTGTTCAATAGATGTCTCAACATTGCCACCAAATTTACAAGCCATTCGTGAGAGCCTCATAGTTCTCTGTGGCCAAGCTGAGGGCTTACTCGAATTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAACCTCTAAACAATCTCACTCTATTTCCATATTACCAAAACTACATCAAATTAGCCAACCTTGAGAACAAGATTTTGAATGACAATGGAATTTTCAATCCAAAGAAGGTAGCTTTTGTTGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAGCACATGAAGGGTACGTATTTCGATAACTACGACATCGACAGCGTGGCAAATGACGTGGCAGGAAAGATCGTGGGGTCGGACACTGAGCTGGAAGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAAAAGAAGAGCTTGGGGGATATGATTGTGTGTTTTTGGCGGCTCTTGTGGGGATGAAGAAGGAAGATAAAGTGAAGATTATAAGGCATTTAAGAAAGTATATGAAAGAAGGTGGGATTTTGGTAGTGCGAAGTGCTAAAGGAGGTAGAGCATTTTTGTATCCTGTGGTTGAAGTTGGAGATTTGGTTGGCTTTGAGATTCTCTCTGTTTTTCATCCCACTGATGATGTTGTTAATTCCGTTATCTTAGCTCGGAAGCCTATGATTATGTGAGTGGATTATGTTTTAATTTATTATGTTGTGTTTTAATTAATTATGTTTCAAAGGGATTGCTAATTATGTTGTCGTATATGCTTGATGTCGTTTTTAAACTTCTTTATTTACAGAAGCAATGTTATTGAGTTTAATTTAATATATCAATTTAAG

mRNA sequence

GAAAAAAAAAACCAAGAGAAAAGAATACAAAAATTATCTTAACACTTCCCTAGCTTCCATTCATTTCCAATTATGGCAGCCCTCCAAATCCATGGCACCAAAACTGAAATCTCCACGGAGTTTCTAGTAGATCGAATCATCGATCTTCACGAAAGCATCTCGAAACTGGAAACCTTGAAACCATGCAAGAAAGTGAACAACCTCTTTACAAACCTAGTGAACTTGTGCATTTTGCCTTGTTCAATAGATGTCTCAACATTGCCACCAAATTTACAAGCCATTCGTGAGAGCCTCATAGTTCTCTGTGGCCAAGCTGAGGGCTTACTCGAATTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAACCTCTAAACAATCTCACTCTATTTCCATATTACCAAAACTACATCAAATTAGCCAACCTTGAGAACAAGATTTTGAATGACAATGGAATTTTCAATCCAAAGAAGGTAGCTTTTGTTGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAGCACATGAAGGGTACGTATTTCGATAACTACGACATCGACAGCGTGGCAAATGACGTGGCAGGAAAGATCGTGGGGTCGGACACTGAGCTGGAAGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAAAAGAAGAGCTTGGGGGATATGATTGTGTGTTTTTGGCGGCTCTTGTGGGGATGAAGAAGGAAGATAAAGTGAAGATTATAAGGCATTTAAGAAAGTATATGAAAGAAGGTGGGATTTTGGTAGTGCGAAGTGCTAAAGGAGGTAGAGCATTTTTGTATCCTGTGGTTGAAGTTGGAGATTTGGTTGGCTTTGAGATTCTCTCTGTTTTTCATCCCACTGATGATGTTGTTAATTCCGTTATCTTAGCTCGGAAGCCTATGATTATGTGAGTGGATTATGTTTTAATTTATTATGTTGTGTTTTAATTAATTATGTTTCAAAGGGATTGCTAATTATGTTGTCGTATATGCTTGATGTCGTTTTTAAACTTCTTTATTTACAGAAGCAATGTTATTGAGTTTAATTTAATATATCAATTTAAG

Coding sequence (CDS)

ATGGCAGCCCTCCAAATCCATGGCACCAAAACTGAAATCTCCACGGAGTTTCTAGTAGATCGAATCATCGATCTTCACGAAAGCATCTCGAAACTGGAAACCTTGAAACCATGCAAGAAAGTGAACAACCTCTTTACAAACCTAGTGAACTTGTGCATTTTGCCTTGTTCAATAGATGTCTCAACATTGCCACCAAATTTACAAGCCATTCGTGAGAGCCTCATAGTTCTCTGTGGCCAAGCTGAGGGCTTACTCGAATTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAACCTCTAAACAATCTCACTCTATTTCCATATTACCAAAACTACATCAAATTAGCCAACCTTGAGAACAAGATTTTGAATGACAATGGAATTTTCAATCCAAAGAAGGTAGCTTTTGTTGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAGCACATGAAGGGTACGTATTTCGATAACTACGACATCGACAGCGTGGCAAATGACGTGGCAGGAAAGATCGTGGGGTCGGACACTGAGCTGGAAGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAAAAGAAGAGCTTGGGGGATATGATTGTGTGTTTTTGGCGGCTCTTGTGGGGATGAAGAAGGAAGATAAAGTGAAGATTATAAGGCATTTAAGAAAGTATATGAAAGAAGGTGGGATTTTGGTAGTGCGAAGTGCTAAAGGAGGTAGAGCATTTTTGTATCCTGTGGTTGAAGTTGGAGATTTGGTTGGCTTTGAGATTCTCTCTGTTTTTCATCCCACTGATGATGTTGTTAATTCCGTTATCTTAGCTCGGAAGCCTATGATTATGTGA

Protein sequence

MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDVSTLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLENKILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARKPMIM

Homology

[Close]

BLAST of Bhi02G000585 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 292.0 bits (746), Expect = 5.3e-79
Identity = 147/267 (55.06%), Postives = 200/267 (74.91%), Query Frame = 0


Query: 18  LVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRESLIV 77
           LV++I DL+E ISKLETLKPC+ V+ LF  LV+ CI P  +IDV+ +  N+Q +R +LI 
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 78  LCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKILNDN-GIFNPKKV 137
           +CG+AEG LE  FS++L+     PL++L LFPYY NY+KL+ LE  +L  N   F P+ V
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFVPRTV 128

Query: 138 AFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRDIV 197
           AF+GSGPLPLTS+++A  H+K + F N+DID  AN VA ++V SD +L  RM F T DI+
Sbjct: 129 AFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIM 188

Query: 198 EVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEV 257
           +V E L G+D VFLAALVGM K++KVK++ HL K+M  G +L++RSA G RAFLYP+VE 
Sbjct: 189 DVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP 248

Query: 258 GDLVGFEILSVFHPTDDVVNSVILARK 282
            DL GFE+LSV+HPTD+V+NS++++RK
Sbjct: 249 CDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Bhi02G000585 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 280.0 bits (715), Expect = 2.1e-75
Identity = 148/269 (55.02%), Postives = 194/269 (72.12%), Query Frame = 0


Query: 16  EFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRESL 75
           E LV  I DL+E ISKLE+LKP + VN LF  LV+ CI P  +IDV+ +   +Q IR +L
Sbjct: 6   EQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNL 65

Query: 76  IVLCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKILNDN-GIFNPK 135
           I +CG AEG LE  FS++L+     PL++L +FPYY NY+KL  LE  +L  N   F PK
Sbjct: 66  IKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFVPK 125

Query: 136 KVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRD 195
            VAF+GSGPLPLTSI++A  H+K T F N+DID  AN +A  +V SD ++  RM F T D
Sbjct: 126 SVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVD 185

Query: 196 IVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVV 255
           I++V E L  +D VFLAALVGM KE+KVK+I HL+K+M  G +L++RSA G RAFLYP+V
Sbjct: 186 IMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIV 245

Query: 256 EVGDLVGFEILSVFHPTDDVVNSVILARK 282
           E  DL GFE+LS++HPTDDV+NSV++++K
Sbjct: 246 EPCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of Bhi02G000585 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 269.6 bits (688), Expect = 2.8e-72
Identity = 138/268 (51.49%), Postives = 189/268 (70.52%), Query Frame = 0


Query: 18  LVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCI-LPCSIDVSTLPPNLQAIRESLIV 77
           +V +IIDL++ ISKL++LKP K V+ LF  LV+ C+    +IDV+ +   ++ +R +LI 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGQAEGLLELEFSTLLSKIPK---PLNNLTLFPYYQNYIKLANLENKILNDNGIFNPKK 137
           LCG+AEG LE  FST+L  + +   PL++L +FPYY NY+KL  LE  +L+ +    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRDI 197
           +AFVGSGP+PLTSI++A  H+  T F N+DIDS AN +A  +V  D +L  RM F T D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVE 257
           +   E L  YD VFLAALVGM KE KVK I HL K+M  G +L++RSA   RAFLYP+V+
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 VGDLVGFEILSVFHPTDDVVNSVILARK 282
             DL GF++L+++HPTDDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of Bhi02G000585 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 265.4 bits (677), Expect = 5.3e-71
Identity = 138/267 (51.69%), Postives = 186/267 (69.66%), Query Frame = 0


Query: 18  LVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCI-LPCSIDVSTL-PPNLQAIRESLI 77
           +V +I+DL+  IS LE+LKP K V+ LF  LV+ C+    +IDV+ +    ++ +R  LI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 78  VLCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKILNDNGIFNPKKV 137
            LCG+AEG LE  FS +L      PLN+L +FPYY NY+KL  LE  +L+ +    P KV
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 138 AFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRDIV 197
           AF+GSGP+PLTSI++A  H+  T F N+DIDS AN +A  +V  D++L  RM F T D++
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 198 EVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEV 257
             KE L  YD VFLAALVGM KE KVK I HL K+M  G ++++RSA G RAFLYP+V+ 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 258 GDLVGFEILSVFHPTDDVVNSVILARK 282
            DL GFE+L+++HP+DDVVNSV++ARK
Sbjct: 248 CDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Bhi02G000585 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 55.5 bits (132), Expect = 8.4e-08
Identity = 28/54 (51.85%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 230 MKEGGILVVRSAKGGRAFLYPVVEVGDLVGFEILSVFHP--TDDVVNSVILARK 282
           M  G +L++RSA+G R+FLY  V+  DL GFE+L ++HP  +D  VNSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARK 54

[Close]

BLAST of Bhi02G000585 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.2e-79
Identity = 150/264 (56.82%), Postives = 192/264 (72.73%), Query Frame = 0


Query: 18  LVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDVSTLPPNLQAIRESLIVL 77
           +V+++ +L+E IS+LE L P K VN LFT+LV+ C+ P  IDVS L   +Q IR  LI L
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNPIDVSKLCQKIQEIRSHLIKL 68

Query: 78  CGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLENKILNDNGIFNPKKVAFV 137
           CGQAEGLLE  FS +LS    PL +L +FPY+ NYIKL+ LE  IL  N    PKK+AF+
Sbjct: 69  CGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIAFI 128

Query: 138 GSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRDIVEVK 197
           GSGPLPLTS+++A +H+K T F NYDID  AN +A  +V +D ++  RM F T D+++V 
Sbjct: 129 GSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVT 188

Query: 198 EELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEVGDL 257
             L  YD VFLAALVGM KEDKVK++ HL KYM  G  L++RSA G RAFLYPV++  DL
Sbjct: 189 CALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPRDL 248

Query: 258 VGFEILSVFHPTDDVVNSVILARK 282
            GFE+LSV+HPTD+V+NSVI+ARK
Sbjct: 249 RGFEVLSVYHPTDEVINSVIIARK 272

[Close]

BLAST of Bhi02G000585 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 7.5e-78
Identity = 147/267 (55.06%), Postives = 200/267 (74.91%), Query Frame = 0


Query: 18  LVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRESLIV 77
           LV++I DL+E ISKLETLKPC+ V+ LF  LV+ CI P  +IDV+ +  N+Q +R +LI 
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 78  LCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKILNDN-GIFNPKKV 137
           +CG+AEG LE  FS++L+     PL++L LFPYY NY+KL+ LE  +L  N   F P+ V
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFVPRTV 128

Query: 138 AFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRDIV 197
           AF+GSGPLPLTS+++A  H+K + F N+DID  AN VA ++V SD +L  RM F T DI+
Sbjct: 129 AFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIM 188

Query: 198 EVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEV 257
           +V E L G+D VFLAALVGM K++KVK++ HL K+M  G +L++RSA G RAFLYP+VE 
Sbjct: 189 DVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEP 248

Query: 258 GDLVGFEILSVFHPTDDVVNSVILARK 282
            DL GFE+LSV+HPTD+V+NS++++RK
Sbjct: 249 CDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Bhi02G000585 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 2.9e-74
Identity = 148/269 (55.02%), Postives = 194/269 (72.12%), Query Frame = 0


Query: 16  EFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRESL 75
           E LV  I DL+E ISKLE+LKP + VN LF  LV+ CI P  +IDV+ +   +Q IR +L
Sbjct: 6   EQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNL 65

Query: 76  IVLCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKILNDN-GIFNPK 135
           I +CG AEG LE  FS++L+     PL++L +FPYY NY+KL  LE  +L  N   F PK
Sbjct: 66  IKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFVPK 125

Query: 136 KVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRD 195
            VAF+GSGPLPLTSI++A  H+K T F N+DID  AN +A  +V SD ++  RM F T D
Sbjct: 126 SVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVD 185

Query: 196 IVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVV 255
           I++V E L  +D VFLAALVGM KE+KVK+I HL+K+M  G +L++RSA G RAFLYP+V
Sbjct: 186 IMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIV 245

Query: 256 EVGDLVGFEILSVFHPTDDVVNSVILARK 282
           E  DL GFE+LS++HPTDDV+NSV++++K
Sbjct: 246 EPCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of Bhi02G000585 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.0e-71
Identity = 138/268 (51.49%), Postives = 189/268 (70.52%), Query Frame = 0


Query: 18  LVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCI-LPCSIDVSTLPPNLQAIRESLIV 77
           +V +IIDL++ ISKL++LKP K V+ LF  LV+ C+    +IDV+ +   ++ +R +LI 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGQAEGLLELEFSTLLSKIPK---PLNNLTLFPYYQNYIKLANLENKILNDNGIFNPKK 137
           LCG+AEG LE  FST+L  + +   PL++L +FPYY NY+KL  LE  +L+ +    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRDI 197
           +AFVGSGP+PLTSI++A  H+  T F N+DIDS AN +A  +V  D +L  RM F T D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVE 257
           +   E L  YD VFLAALVGM KE KVK I HL K+M  G +L++RSA   RAFLYP+V+
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 VGDLVGFEILSVFHPTDDVVNSVILARK 282
             DL GF++L+++HPTDDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of Bhi02G000585 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.5e-70
Identity = 138/267 (51.69%), Postives = 186/267 (69.66%), Query Frame = 0


Query: 18  LVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCI-LPCSIDVSTL-PPNLQAIRESLI 77
           +V +I+DL+  IS LE+LKP K V+ LF  LV+ C+    +IDV+ +    ++ +R  LI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 78  VLCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKILNDNGIFNPKKV 137
            LCG+AEG LE  FS +L      PLN+L +FPYY NY+KL  LE  +L+ +    P KV
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 138 AFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDTELEGRMKFWTRDIV 197
           AF+GSGP+PLTSI++A  H+  T F N+DIDS AN +A  +V  D++L  RM F T D++
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 198 EVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEV 257
             KE L  YD VFLAALVGM KE KVK I HL K+M  G ++++RSA G RAFLYP+V+ 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 258 GDLVGFEILSVFHPTDDVVNSVILARK 282
            DL GFE+L+++HP+DDVVNSV++ARK
Sbjct: 248 CDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Bhi02G000585 vs. NCBI nr
Match: XP_038880344.1 (nicotianamine synthase-like [Benincasa hispida])

HSP 1 Score: 565.1 bits (1455), Expect = 3.5e-157
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN
Sbjct: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT
Sbjct: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARKPMIM 286
           AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARKPMIM
Sbjct: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARKPMIM 285

[Close]

BLAST of Bhi02G000585 vs. NCBI nr
Match: XP_008453616.1 (PREDICTED: nicotianamine synthase-like [Cucumis melo] >TYK11847.1 nicotianamine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 516.5 bits (1329), Expect = 1.4e-142
Identity = 257/281 (91.46%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ HG + EIS  FLVDRIIDLH+SI KLETL+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRESLI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KILNDNGI NPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYD+DSVANDVA KIVGSD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARK 282
           A+GGRAFLYPVVEVGDLVGFEILS+FHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Bhi02G000585 vs. NCBI nr
Match: KAA0058291.1 (nicotianamine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 515.0 bits (1325), Expect = 4.1e-142
Identity = 256/281 (91.10%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ HG + EIS  FLVDRIIDLH+SI KLETL+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRESLI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KILNDNGI NPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYD+DSVANDVA KIVGSD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARK 282
           A+GGRAFLYPVV+VGDLVGFEILS+FHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Bhi02G000585 vs. NCBI nr
Match: XP_004146360.2 (probable nicotianamine synthase 4 [Cucumis sativus] >KGN65443.1 hypothetical protein Csa_020027 [Cucumis sativus])

HSP 1 Score: 513.8 bits (1322), Expect = 9.2e-142
Identity = 257/281 (91.46%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQIHGT+ EIS  FLVDRIIDLHESISKLETL+PCKKVNNLFTNLV LCILPCSIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQ IRESLI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KILNDNGI NPKKVAFVGSGPLPLTSIIMAMQHMKGT+FDNYD+DSVANDVA KIVGSD+
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           +LEGRMKF + DIV+VKEELGGYDCVFLAALVGM KE+KVKIIRHLRKYMKEGGIL+VRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARK 282
           AKGGRAFLYPVVEV DLVGFEILS+FHPTDDVVNSVIL RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of Bhi02G000585 vs. NCBI nr
Match: KAG6589715.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 481.9 bits (1239), Expect = 3.9e-132
Identity = 236/285 (82.81%), Postives = 265/285 (92.98%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQI GT+T+IS EFL+ R+IDLH SIS+LETLKPCK+VN LFTNLV LCILPCSIDV
Sbjct: 1   MAALQILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STL P+LQAIRESLI+LCG+AEGLLELEFST+L KIPKPLNNLTLFPYY NYIKLA+LE 
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KIL DNG+ NPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DS+AN+VAGKIV SD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEV EEL GYDCVFLAALVG+ KEDKVKI+ HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARKPMIM 286
           A+GGRAFLYPVVEV D+VGF+ILS+FHP+DDVVNSV+LARKP+++
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Bhi02G000585 vs. ExPASy TrEMBL
Match: A0A5D3CIT2 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00680 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 6.9e-143
Identity = 257/281 (91.46%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ HG + EIS  FLVDRIIDLH+SI KLETL+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRESLI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KILNDNGI NPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYD+DSVANDVA KIVGSD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARK 282
           A+GGRAFLYPVVEVGDLVGFEILS+FHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Bhi02G000585 vs. ExPASy TrEMBL
Match: A0A1S3BXH0 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 6.9e-143
Identity = 257/281 (91.46%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ HG + EIS  FLVDRIIDLH+SI KLETL+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRESLI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KILNDNGI NPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYD+DSVANDVA KIVGSD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARK 282
           A+GGRAFLYPVVEVGDLVGFEILS+FHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Bhi02G000585 vs. ExPASy TrEMBL
Match: A0A5A7UXS8 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006240 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.0e-142
Identity = 256/281 (91.10%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ HG + EIS  FLVDRIIDLH+SI KLETL+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRESLI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KILNDNGI NPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYD+DSVANDVA KIVGSD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARK 282
           A+GGRAFLYPVV+VGDLVGFEILS+FHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Bhi02G000585 vs. ExPASy TrEMBL
Match: A0A0A0LUJ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.5e-142
Identity = 257/281 (91.46%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQIHGT+ EIS  FLVDRIIDLHESISKLETL+PCKKVNNLFTNLV LCILPCSIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQ IRESLI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KILNDNGI NPKKVAFVGSGPLPLTSIIMAMQHMKGT+FDNYD+DSVANDVA KIVGSD+
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           +LEGRMKF + DIV+VKEELGGYDCVFLAALVGM KE+KVKIIRHLRKYMKEGGIL+VRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARK 282
           AKGGRAFLYPVVEV DLVGFEILS+FHPTDDVVNSVIL RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of Bhi02G000585 vs. ExPASy TrEMBL
Match: A0A6J1JBQ8 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 5.5e-132
Identity = 236/285 (82.81%), Postives = 263/285 (92.28%), Query Frame = 0


Query: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60
           M ALQI GT+T+IS E L+ R+IDLH SIS+LETLKPCK+VN LFTNLV  CILPCSIDV
Sbjct: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPP+LQAIRESLI+ CG+AEGLLELEFST+L KIPKPLNNLTLFPYY NYIKLA+LE 
Sbjct: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180
           KIL DNG+ NPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDIDS+AN+VAGKIV SD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEV EEL GYDCVFLAALVG+KKEDKVKI+ HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARKPMIM 286
           AKGGRAFLYPVVEV D+VGF+ILS+FHP+DDVVNSV+LARKP+++
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 285

The following BLAST results are available for this feature:

BLAST of Bhi02G000585 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	5.3e-79	55.06	nicotianamine synthase 4	[more]
AT1G09240.1	2.1e-75	55.02	nicotianamine synthase 3	[more]
AT5G04950.1	2.8e-72	51.49	nicotianamine synthase 1	[more]
AT5G56080.1	5.3e-71	51.69	nicotianamine synthase 2	[more]
AT4G26483.1	8.4e-08	51.85	nicotianamine synthases	[more]

BLAST of Bhi02G000585 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	5.2e-79	56.82	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	7.5e-78	55.06	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	2.9e-74	55.02	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	4.0e-71	51.49	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	7.5e-70	51.69	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Bhi02G000585 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880344.1	3.5e-157	100.00	nicotianamine synthase-like [Benincasa hispida]	[more]
XP_008453616.1	1.4e-142	91.46	PREDICTED: nicotianamine synthase-like [Cucumis melo] >TYK11847.1 nicotianamine ...	[more]
KAA0058291.1	4.1e-142	91.10	nicotianamine synthase-like [Cucumis melo var. makuwa]	[more]
XP_004146360.2	9.2e-142	91.46	probable nicotianamine synthase 4 [Cucumis sativus] >KGN65443.1 hypothetical pro...	[more]
KAG6589715.1	3.9e-132	82.81	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Bhi02G000585 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CIT2	6.9e-143	91.46	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BXH0	6.9e-143	91.46	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1	[more]
A0A5A7UXS8	2.0e-142	91.10	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A0A0LUJ7	4.5e-142	91.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1	[more]
A0A6J1JBQ8	5.5e-132	82.81	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 14..282 e-value: 9.5E-60 score: 204.0
None	No IPR available	PANTHER	PTHR32266:SF11	NICOTIANAMINE SYNTHASE	coord: 1..283
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 163..238 e-value: 0.00814416 score: 33.5575
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 15..283 e-value: 8.5E-107 score: 356.6
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..283
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 10..285 score: 48.977352
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 112..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M000585	Bhi02M000585	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity