Bhi02G000216 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000216
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionProtein of unknown function (DUF962)
Locationchr2: 5861011 .. 5864196 (+)
RNA-Seq ExpressionBhi02G000216
SyntenyBhi02G000216
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAATTTGTCAATATTTAATGTTTCCTACCGAATTTCTCCACTTCAGACTGCTTACCTTCCCGAATTATAAAGTGATTAATTTAGCTGACGATCTTTAACCAAACCCATTAATATATCCTTCTCTGATCGTTATTTATTTTCTAACCCTCATAATCTCTCCGATCATCTTACCTTACACGCCTCCCCTCTTCTGAGAATTTTCCGGCAATCGTTTCGTTTCGTATTTGGTTTGTCTGTTTATTTTCCCAGAAGATTTTATTATGGGGAAGTCTGGATTGTTTGATCTGGAGAGGCACTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATATTCTATTTGTGTGGCCGATTTTCTTCACCAGTCTTATGTATTTGTATTTCACACCTTCATTCTACACTATTCCCAAATCCCCTTGTGGGTTTGACCATGGCTTGGTATTGAACTTCGGATTTCTTTTCACTCTAATCTATGCTGCATCTTATGTGGTTTTCGATAAGAAAGCAGGTTCCATGGCCGCTTTGCTTTGTTTCGTTTGTTGGGTTGGAGCAAGCTTCATCGCCAACAGACTTGGTTATTCCCAGACTTGGAAGGTAATATAATTTCTGGGTCCTTGGTTTTTGAAGTAAATTAGTTTGTTTCCTTACCCCATTTGGATCGCTAGTCCTTTTGTTATTAATCTTTCTAATGGATCATTTGTTGAGTGGATTGAAATTTCCTTGATGGGTTTGCTTGTAAATGGGATTAGTGGCAAGTGTTGGAAACAGCTCAATCTGATTTATCTGGTTGGGAATTTGGTAGATATGTTAAAGTTTGACATCTCAAATGAGAGTTTGTTTGTTTAATATTTATTGTATATCAGGTAGTACTGGCGGCTCAGTTGTTCTGTTGGACCAATCAGTTTATAGGACATGGAGTGTTTGAGGTAGACAGGTTACTTCATCTCAGTGAATTGATTCCATTTCTTTTTTATTCTTAGGCCTCTTGATTTAATTTCCTCTTTTTGATAGAAACGAGCACCAGCCTTGTTAGACAATCTTGCACAAGCTTTTCTAATGGCTCCATTCTTTGTAGTTTTGGAGGTATTCTTTCTTTGGTTCTCCCCTGTTATGAGAGTTATTTGTTAGTGTGGATAATGAAGCAACTCTGTTGTTTCTGATGTTTGGTTGCAGGTTCTTCAAAGTTTATTCAAATATGAACCATATCCAGGATTTAGTGCGAGTGTGCAAGCAAAGATAAAAGCAGATATAGAAGAATGGAAAGAAAAGAAGAAAAAACTGTCATAGGTTGAATTGCCTAGACTTCAGAACTACTCCTGTGTTGGATTTCCCCTTGTAAAAATAATGTTGAAACTAACAACTTCAGTTGAAAAATCTTTCTGTGATATTGTCGTACATGTTCTGCTTTTCAACCTTGATTTACCATTCTAATATGATGTGTAACAGAATGTATTAAAAGAATTAAGAGGACACAGGTGTGAGGACTTACATGTTAAATAAGAATTCAAACGGATGCACATTGAAAACTCCGATATGACTCTCGTTCCTTTCTGCATCATTACAATTGATTAATTTCCAAATAACTATTACCAGGCTCTGGTTTCTGGACTCCTTTTCTTTGCATTGATTTTCTGGTCTTTATCATTTCATTCCATTGACCAGAGCTTGCATACATATTTGCAAGAAGAACATAATCACTACTATGATCTGGCTCTATCTCCAGAACATGGCTCCTTACTCTCTCCCCGAGTTTAACATTTCCATGCATCCGACAGGCTGCAAGTAACGTTCTCCATATAACAGCATTGCACTCCATTGGCATGCTCCTTATCAGCTGATAAGCTTCCTCTACAAATCCAGCTCGTCCCAGAATGTCCACCATGGATCCATAATGCTTAACTGTGGGTTGGATATTGAAGTGTTTTGTCATTAGATGAAAATACTCTCTCCCTTCCTCCACTTTTCCTCCATGGTTACAAGCACACAATACTCCCAAGAAAGTTACACCATCAGGGGTCTCCACCTTCTTTGCTAACATGTTTGAGAATAGTGTCAGTGCATCCTCTGCATCACCATGTGTTGCTAATCCCATGATCATTGTGTTCCATGTTACTACGTTCTTGTCACTCATCGCATTAAACATCTCGCGAGCATCTTCAACCGCTCCACACTTGGCATACATGTCGATCAATGAATTGAGAACAGCTATAGTCTTTCCTTTATCGTTACGCTTCACATGGGAATGAACCCACCTCCCAAAGTCCAGTGCACCTAATGCAGAACATGCTGAGACCGTCACAACCAATGTGGCTTCATCAGGCTCTACGCCACTCTGCAACATTTGAAGAAACAATCCAAGTGCTTCGCTGTACATCCCACAAGAGACATGACAGTCAATTACAGCATTCCAAGCCACTAAATCTGGTTTGGGCATTTCATCAAACAGGTTGCGTGCTATTTTGACATCTTTTAACCTGCCATACATATGTATAAGCGTGTTCCTCACATACACATGGGAATCAAGGCCAACTTTCAGGATATTAACATGTAATTGCTTTCCCAACATAATTGAACCCGACTGCCCAGAAATCTTCAGCAAGAAAGAAAAAGTGAAATTGTCTGCTGCTATTCCCTTCTCTAGCATTCTCTTGTAGAACTCAAACGCCTTTAACAGTTTACTAATTCTTCCAAATCCCCTGATCATTGTATTCCAAAGAAACCCATCTGGGTTTTCGATTCCGTTGAAAACAACAACAGCGTAATTCATGTCGCCATAGTTCGAAACTGCACAAAACTCAATGAGTTTGCCAATAACGAAGAGATTCTGATCGAAACCTAACTGGATAATACGAGCATGGAGTTGATTCAAGTCTTTCACGGTGGAACATTGCTTGGAAAGAGACAGAAATCTTTGCTCCTTGGCAAAATAGCTGCCATTGGGTATTGTATCAGTGGCTGTTTTTCGATCTGAACTCCAAACGAACTTGGGGACGAGCAATTGAAAATGTGGAAATTTGAACTTTACTTGGGAGAACAGAGAGAGTGCCAAGTTCGCTTTTCTGCTGGTAATAACGATCATAACAATTTTGTTCAATCAAACGCTAGCAAATTCCGGCGCTTAAGTAAAATTCTTCCGTTCTGTCAGCACAGAAGTTGAGGGAAAATTATC

mRNA sequence

CCAAAATTTGTCAATATTTAATGTTTCCTACCGAATTTCTCCACTTCAGACTGCTTACCTTCCCGAATTATAAAGTGATTAATTTAGCTGACGATCTTTAACCAAACCCATTAATATATCCTTCTCTGATCGTTATTTATTTTCTAACCCTCATAATCTCTCCGATCATCTTACCTTACACGCCTCCCCTCTTCTGAGAATTTTCCGGCAATCGTTTCGTTTCGTATTTGGTTTGTCTGTTTATTTTCCCAGAAGATTTTATTATGGGGAAGTCTGGATTGTTTGATCTGGAGAGGCACTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATATTCTATTTGTGTGGCCGATTTTCTTCACCAGTCTTATGTATTTGTATTTCACACCTTCATTCTACACTATTCCCAAATCCCCTTGTGGGTTTGACCATGGCTTGGTATTGAACTTCGGATTTCTTTTCACTCTAATCTATGCTGCATCTTATGTGGTTTTCGATAAGAAAGCAGGTTCCATGGCCGCTTTGCTTTGTTTCGTTTGTTGGGTTGGAGCAAGCTTCATCGCCAACAGACTTGGTTATTCCCAGACTTGGAAGGTAGTACTGGCGGCTCAGTTGTTCTGTTGGACCAATCAGTTTATAGGACATGGAGTGTTTGAGAAACGAGCACCAGCCTTGTTAGACAATCTTGCACAAGCTTTTCTAATGGCTCCATTCTTTGTAGTTTTGGAGGTTCTTCAAAGTTTATTCAAATATGAACCATATCCAGGATTTAGTGCGAGTGTGCAAGCAAAGATAAAAGCAGATATAGAAGAATGGAAAGAAAAGAAGAAAAAACTGTCATAGGTTGAATTGCCTAGACTTCAGAACTACTCCTGTGTTGGATTTCCCCTTGTAAAAATAATGTTGAAACTAACAACTTCAGTTGAAAAATCTTTCTGTGATATTGTCGTACATGTTCTGCTTTTCAACCTTGATTTACCATTCTAATATGATGTGTAACAGAATGTATTAAAAGAATTAAGAGGACACAGGTGTGAGGACTTACATGTTAAATAAGAATTCAAACGGATGCACATTGAAAACTCCGATATGACTCTCGTTCCTTTCTGCATCATTACAATTGATTAATTTCCAAATAACTATTACCAGGCTCTGGTTTCTGGACTCCTTTTCTTTGCATTGATTTTCTGGTCTTTATCATTTCATTCCATTGACCAGAGCTTGCATACATATTTGCAAGAAGAACATAATCACTACTATGATCTGGCTCTATCTCCAGAACATGGCTCCTTACTCTCTCCCCGAGTTTAACATTTCCATGCATCCGACAGGCTGCAAGTAACGTTCTCCATATAACAGCATTGCACTCCATTGGCATGCTCCTTATCAGCTGATAAGCTTCCTCTACAAATCCAGCTCGTCCCAGAATGTCCACCATGGATCCATAATGCTTAACTGTGGGTTGGATATTGAAGTGTTTTGTCATTAGATGAAAATACTCTCTCCCTTCCTCCACTTTTCCTCCATGGTTACAAGCACACAATACTCCCAAGAAAGTTACACCATCAGGGGTCTCCACCTTCTTTGCTAACATGTTTGAGAATAGTGTCAGTGCATCCTCTGCATCACCATGTGTTGCTAATCCCATGATCATTGTGTTCCATGTTACTACGTTCTTGTCACTCATCGCATTAAACATCTCGCGAGCATCTTCAACCGCTCCACACTTGGCATACATGTCGATCAATGAATTGAGAACAGCTATAGTCTTTCCTTTATCGTTACGCTTCACATGGGAATGAACCCACCTCCCAAAGTCCAGTGCACCTAATGCAGAACATGCTGAGACCGTCACAACCAATGTGGCTTCATCAGGCTCTACGCCACTCTGCAACATTTGAAGAAACAATCCAAGTGCTTCGCTGTACATCCCACAAGAGACATGACAGTCAATTACAGCATTCCAAGCCACTAAATCTGGTTTGGGCATTTCATCAAACAGGTTGCGTGCTATTTTGACATCTTTTAACCTGCCATACATATGTATAAGCGTGTTCCTCACATACACATGGGAATCAAGGCCAACTTTCAGGATATTAACATGTAATTGCTTTCCCAACATAATTGAACCCGACTGCCCAGAAATCTTCAGCAAGAAAGAAAAAGTGAAATTGTCTGCTGCTATTCCCTTCTCTAGCATTCTCTTGTAGAACTCAAACGCCTTTAACAGTTTACTAATTCTTCCAAATCCCCTGATCATTGTATTCCAAAGAAACCCATCTGGGTTTTCGATTCCGTTGAAAACAACAACAGCGTAATTCATGTCGCCATAGTTCGAAACTGCACAAAACTCAATGAGTTTGCCAATAACGAAGAGATTCTGATCGAAACCTAACTGGATAATACGAGCATGGAGTTGATTCAAGTCTTTCACGGTGGAACATTGCTTGGAAAGAGACAGAAATCTTTGCTCCTTGGCAAAATAGCTGCCATTGGGTATTGTATCAGTGGCTGTTTTTCGATCTGAACTCCAAACGAACTTGGGGACGAGCAATTGAAAATGTGGAAATTTGAACTTTACTTGGGAGAACAGAGAGAGTGCCAAGTTCGCTTTTCTGCTGGTAATAACGATCATAACAATTTTGTTCAATCAAACGCTAGCAAATTCCGGCGCTTAAGTAAAATTCTTCCGTTCTGTCAGCACAGAAGTTGAGGGAAAATTATC

Coding sequence (CDS)

ATGGGGAAGTCTGGATTGTTTGATCTGGAGAGGCACTTCGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATATTCTATTTGTGTGGCCGATTTTCTTCACCAGTCTTATGTATTTGTATTTCACACCTTCATTCTACACTATTCCCAAATCCCCTTGTGGGTTTGACCATGGCTTGGTATTGAACTTCGGATTTCTTTTCACTCTAATCTATGCTGCATCTTATGTGGTTTTCGATAAGAAAGCAGGTTCCATGGCCGCTTTGCTTTGTTTCGTTTGTTGGGTTGGAGCAAGCTTCATCGCCAACAGACTTGGTTATTCCCAGACTTGGAAGGTAGTACTGGCGGCTCAGTTGTTCTGTTGGACCAATCAGTTTATAGGACATGGAGTGTTTGAGAAACGAGCACCAGCCTTGTTAGACAATCTTGCACAAGCTTTTCTAATGGCTCCATTCTTTGTAGTTTTGGAGGTTCTTCAAAGTTTATTCAAATATGAACCATATCCAGGATTTAGTGCGAGTGTGCAAGCAAAGATAAAAGCAGATATAGAAGAATGGAAAGAAAAGAAGAAAAAACTGTCATAG

Protein sequence

MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGFDHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLAAQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQAKIKADIEEWKEKKKKLS
Homology
BLAST of Bhi02G000216 vs. TAIR 10
Match: AT1G18720.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 253.1 bits (645), Expect = 1.9e-67
Identity = 130/199 (65.33%), Postives = 154/199 (77.39%), Query Frame = 0

Query: 5   GLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLY-FTPSFYTIPKSPCGFDHG 64
           GLFDLE+HFAFYGAYHSNP+NI IHI+FVWPIFF+ L+ L+  TP F     S  GF   
Sbjct: 6   GLFDLEKHFAFYGAYHSNPINILIHIIFVWPIFFSVLLLLHSSTPIF---DPSQLGFSQS 65

Query: 65  LVL------NFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKV 124
           L L      N GF+F LIYA  Y+  DKK+G +AAL+CF CWVG+SF+A RLG S   KV
Sbjct: 66  LTLDGVLRFNVGFIFALIYALFYIGLDKKSGFVAALMCFSCWVGSSFLAVRLGSSLALKV 125

Query: 125 VLAAQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSA 184
            LA+QL CWT QF+GHGVFEKRAPALLDNL QAFLMAPFFV+LEVLQS+F YEPYPGF A
Sbjct: 126 GLASQLLCWTGQFVGHGVFEKRAPALLDNLLQAFLMAPFFVLLEVLQSVFGYEPYPGFQA 185

Query: 185 SVQAKIKADIEEWKEKKKK 197
            V AK+++DI+E++ KK+K
Sbjct: 186 RVNAKVESDIKEYRAKKQK 201

BLAST of Bhi02G000216 vs. TAIR 10
Match: AT1G74440.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 249.2 bits (635), Expect = 2.7e-66
Identity = 124/198 (62.63%), Postives = 151/198 (76.26%), Query Frame = 0

Query: 5   GLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCG----- 64
           GL DLE+HFAFYGAYHSNP+NI IH LFVWP  F +L++LY TP    +  S  G     
Sbjct: 6   GLLDLEKHFAFYGAYHSNPINIIIHTLFVWPNVFATLLFLYSTPPI--LDHSQLGFLKSL 65

Query: 65  -FDHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVV 124
            FD  L L+ GF  T+ YA  Y+  DKK+G +AALLCF CW+G+SF+A RLG+S T KV 
Sbjct: 66  TFDGVLRLDIGFTLTVTYAVFYICLDKKSGVLAALLCFSCWIGSSFLAARLGHSLTLKVG 125

Query: 125 LAAQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSAS 184
           +A+QL CWT QF+GHG+FEKRAPALLDNL QAFLM PFFV+LEVLQS+F YEPYPGF A 
Sbjct: 126 VASQLLCWTGQFLGHGLFEKRAPALLDNLLQAFLMGPFFVLLEVLQSVFGYEPYPGFKAR 185

Query: 185 VQAKIKADIEEWKEKKKK 197
           V +KI++ I+EW+EKKK+
Sbjct: 186 VDSKIESGIKEWREKKKQ 201

BLAST of Bhi02G000216 vs. ExPASy Swiss-Prot
Match: O13737 (2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mpo1 PE=3 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 2.7e-18
Identity = 66/181 (36.46%), Postives = 92/181 (50.83%), Query Frame = 0

Query: 9   LERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGFDHGLVLNF 68
           L R ++FY AYHSNPVNI IH + +  +  T+L+ L+      T+  S       L +N 
Sbjct: 4   LSRSYSFYAAYHSNPVNIKIHQVCIPLLLLTALVLLH--NFVITLINSK------LQINV 63

Query: 69  GFLFTLIYAASYVVFDKKAGSM-AALLCFVCWVGASFIANRLGYSQTWKVVLAAQLFCWT 128
             L  L Y   YV  D   G + + +L    ++  S +      S   +      + CW 
Sbjct: 64  AHLVGLAYQIFYVTLDPLDGLLYSPVLYLFSYILPSKLFTIFSRSLVNRSAAVVHVICWI 123

Query: 129 NQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQAKIKADI 188
            QFIGHGVFEKR PALLDNL Q+  +AP F  LE         P+ G+  SV +KI+A+I
Sbjct: 124 LQFIGHGVFEKRKPALLDNLIQSLFIAPLFAFLET-------GPFVGYYPSVVSKIRANI 169

BLAST of Bhi02G000216 vs. ExPASy Swiss-Prot
Match: P25338 (2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MPO1 PE=1 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 5.1e-17
Identity = 62/184 (33.70%), Postives = 89/184 (48.37%), Query Frame = 0

Query: 5   GLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGFDHGL 64
           GL DL     FY  YH NP N+ IH +FV  I F+    L+    + +I          L
Sbjct: 4   GLLDLRSQLGFYKFYHHNPKNVLIHSIFVPTILFSGSCMLHRVKIYQSI---------SL 63

Query: 65  VLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLAAQLF 124
                 LF++ Y   Y+     AG +  LL        + I +R+    T+K  L     
Sbjct: 64  TAVLSVLFSIFYCLLYLPTGLLAGVLLLLL------NLALIDHRV--DLTFKQELGLFTI 123

Query: 125 CWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQAKIK 184
            W  QF+GHGVFEKR PAL+DNL Q+ ++AP+F++ E L  L       GF   ++A ++
Sbjct: 124 GWIFQFVGHGVFEKRRPALIDNLVQSLVLAPYFIMFEFLFKL-------GFMPRLKATLE 163

Query: 185 ADIE 189
            D+E
Sbjct: 184 HDLE 163

BLAST of Bhi02G000216 vs. NCBI nr
Match: XP_038880038.1 (2-hydroxy-palmitic acid dioxygenase MPO1 [Benincasa hispida])

HSP 1 Score: 404.4 bits (1038), Expect = 5.5e-109
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKIKADIEEWKEKKKKLS
Sbjct: 181 AKIKADIEEWKEKKKKLS 198

BLAST of Bhi02G000216 vs. NCBI nr
Match: XP_022933403.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita moschata] >KAG7021523.1 SPAC16E8.02 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 387.5 bits (994), Expect = 6.9e-104
Identity = 185/198 (93.43%), Postives = 195/198 (98.48%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+  FDLERHFAFYGAYHSNPVNIFIH+LFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTLIYAASYV+FDK+AGSMAALLCFVCWVGASFI+NRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKI+ADI+EWKEKK+KLS
Sbjct: 181 AKIEADIKEWKEKKEKLS 198

BLAST of Bhi02G000216 vs. NCBI nr
Match: XP_004138174.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis sativus] >KGN63680.1 hypothetical protein Csa_014278 [Cucumis sativus])

HSP 1 Score: 386.0 bits (990), Expect = 2.0e-103
Identity = 186/198 (93.94%), Postives = 194/198 (97.98%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+GLFDLE+ FAFYGAYHSNP+NIFIH+LFVWPIFFTSLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTL+YAA YVVFDK+AGSMAALLCFVCWVGASFIAN+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASFIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKIKADI EWKE K+KLS
Sbjct: 181 AKIKADIREWKETKEKLS 198

BLAST of Bhi02G000216 vs. NCBI nr
Match: XP_008453216.1 (PREDICTED: uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis melo] >KAA0057952.1 putative endoplasmic reticulum membrane protein [Cucumis melo var. makuwa])

HSP 1 Score: 385.6 bits (989), Expect = 2.6e-103
Identity = 185/198 (93.43%), Postives = 194/198 (97.98%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+GLFDLE+ FAFYGAYHSNP+NIFIH+LFVWPIFFTSLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGF FTL+YAA YVVFDK+AGSMAALLCFVCWVGAS+IAN+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKIKADI EWKEKK+KLS
Sbjct: 181 AKIKADIREWKEKKEKLS 198

BLAST of Bhi02G000216 vs. NCBI nr
Match: XP_023531593.1 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 385.2 bits (988), Expect = 3.4e-103
Identity = 183/195 (93.85%), Postives = 192/195 (98.46%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+  FDLERHFAFYGAYHSNP+NIFIH+LFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPINIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTLIYAASYV+FDKKAGSMAALLCFVCWVGASFI+NRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKK 196
           AKI+ADI+EWKEKKK
Sbjct: 181 AKIEADIKEWKEKKK 195

BLAST of Bhi02G000216 vs. ExPASy TrEMBL
Match: A0A6J1F4N0 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita moschata OX=3662 GN=LOC111440831 PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 3.4e-104
Identity = 185/198 (93.43%), Postives = 195/198 (98.48%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+  FDLERHFAFYGAYHSNPVNIFIH+LFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHFAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTLIYAASYV+FDK+AGSMAALLCFVCWVGASFI+NRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLIYAASYVIFDKRAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKI+ADI+EWKEKK+KLS
Sbjct: 181 AKIEADIKEWKEKKEKLS 198

BLAST of Bhi02G000216 vs. ExPASy TrEMBL
Match: A0A0A0LP54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009840 PE=4 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 9.8e-104
Identity = 186/198 (93.94%), Postives = 194/198 (97.98%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+GLFDLE+ FAFYGAYHSNP+NIFIH+LFVWPIFFTSLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTL+YAA YVVFDK+AGSMAALLCFVCWVGASFIAN+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASFIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKIKADI EWKE K+KLS
Sbjct: 181 AKIKADIREWKETKEKLS 198

BLAST of Bhi02G000216 vs. ExPASy TrEMBL
Match: A0A5A7UQ13 (Putative endoplasmic reticulum membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002550 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.3e-103
Identity = 185/198 (93.43%), Postives = 194/198 (97.98%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+GLFDLE+ FAFYGAYHSNP+NIFIH+LFVWPIFFTSLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGF FTL+YAA YVVFDK+AGSMAALLCFVCWVGAS+IAN+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKIKADI EWKEKK+KLS
Sbjct: 181 AKIKADIREWKEKKEKLS 198

BLAST of Bhi02G000216 vs. ExPASy TrEMBL
Match: A0A1S3BV50 (uncharacterized endoplasmic reticulum membrane protein YGL010W OS=Cucumis melo OX=3656 GN=LOC103494003 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.3e-103
Identity = 185/198 (93.43%), Postives = 194/198 (97.98%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+GLFDLE+ FAFYGAYHSNP+NIFIH+LFVWPIFFTSLMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTGLFDLEKQFAFYGAYHSNPMNIFIHVLFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGF FTL+YAA YVVFDK+AGSMAALLCFVCWVGAS+IAN+LGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFFFTLMYAAYYVVFDKRAGSMAALLCFVCWVGASYIANKLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ
Sbjct: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKIKADI EWKEKK+KLS
Sbjct: 181 AKIKADIREWKEKKEKLS 198

BLAST of Bhi02G000216 vs. ExPASy TrEMBL
Match: A0A6J1IBP8 (uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita maxima OX=3661 GN=LOC111471498 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.0e-102
Identity = 181/198 (91.41%), Postives = 192/198 (96.97%), Query Frame = 0

Query: 1   MGKSGLFDLERHFAFYGAYHSNPVNIFIHILFVWPIFFTSLMYLYFTPSFYTIPKSPCGF 60
           MGK+  FDLERH+AFYGAYHSNPVNIFIH+LFVWPIFFT+LMYLYFTPSFYTIPKSPCGF
Sbjct: 1   MGKTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGF 60

Query: 61  DHGLVLNFGFLFTLIYAASYVVFDKKAGSMAALLCFVCWVGASFIANRLGYSQTWKVVLA 120
           DHGLVLNFGFLFTL YAASYV+FDKKAGSMAALLCFVCWVGASFI+NRLGYSQTWKVVLA
Sbjct: 61  DHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLA 120

Query: 121 AQLFCWTNQFIGHGVFEKRAPALLDNLAQAFLMAPFFVVLEVLQSLFKYEPYPGFSASVQ 180
           AQLFCWTNQ IGHGVFEKRAPALLDNLAQAFLMAPFFV LEVLQ+LFKYEPYPGFS+SVQ
Sbjct: 121 AQLFCWTNQIIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSSSVQ 180

Query: 181 AKIKADIEEWKEKKKKLS 199
           AKI+ADI+EWKE K+KLS
Sbjct: 181 AKIEADIKEWKEMKEKLS 198

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G18720.11.9e-6765.33Protein of unknown function (DUF962) [more]
AT1G74440.12.7e-6662.63Protein of unknown function (DUF962) [more]
Match NameE-valueIdentityDescription
O137372.7e-1836.462-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 97... [more]
P253385.1e-1733.702-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
XP_038880038.15.5e-109100.002-hydroxy-palmitic acid dioxygenase MPO1 [Benincasa hispida][more]
XP_022933403.16.9e-10493.43uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita mosch... [more]
XP_004138174.12.0e-10393.94uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucumis sativus]... [more]
XP_008453216.12.6e-10393.43PREDICTED: uncharacterized endoplasmic reticulum membrane protein YGL010W [Cucum... [more]
XP_023531593.13.4e-10393.85uncharacterized endoplasmic reticulum membrane protein C16E8.02 [Cucurbita pepo ... [more]
Match NameE-valueIdentityDescription
A0A6J1F4N03.4e-10493.43uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita mos... [more]
A0A0A0LP549.8e-10493.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009840 PE=4 SV=1[more]
A0A5A7UQ131.3e-10393.43Putative endoplasmic reticulum membrane protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BV501.3e-10393.43uncharacterized endoplasmic reticulum membrane protein YGL010W OS=Cucumis melo O... [more]
A0A6J1IBP87.0e-10291.41uncharacterized endoplasmic reticulum membrane protein C16E8.02 OS=Cucurbita max... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 180..198
NoneNo IPR availablePANTHERPTHR28026:SF8YGL010W-LIKE PROTEINcoord: 1..197
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePFAMPF06127DUF962coord: 8..165
e-value: 3.3E-29
score: 100.8
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePANTHERPTHR28026DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)coord: 1..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000216Bhi02M000216mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046521 sphingoid catabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane