Bhi01G001514 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G001514
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	EF hand calcium-binding protein family
Location	chr1: 44262244 .. 44263766 (-)
RNA-Seq Expression	Bhi01G001514
Synteny	Bhi01G001514

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATAAAAAATTAAAGTTTCTCTCGGTTTTCAAATTTCAAAATCCCATTGATCTCTTTTGATCCTGAATCTTCACTCACGCCCATGGCCAAGAAGAACAACTCCCTTTCAATTTCAAATCCTGGATCCTCCTTGGGCTCCATGGACGACGTCATCAAGGTCTTCAACAAGTTCGACAAGAACGGCGACGGCAAGATTTCCGTTAACGAGCTCGGCGCTGCCCTCAGCGAACTTGGCGGCAAGATCTCCACCGACGAGATCCGCCGCATTATGTCTGAGATCGACAAGGACGGCGACGGATTCATCGACCTCGATGAGTTCGCCGAGTTTCATCAGGACGCCTCGTCTTCCAGCGGATGTAACAAAGATCTGCAGGACGCTTTCGATCTGTACGATATCGATAAGAACGGCTTAATCTCTGCTAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATTAGTTCCGTCGATGTCGACGGTGATGGACATGTAAATTTTGAGGAATTCAAGAAGATGATGACTCGCTCTTAGTCGTCTCCGGCAGTAGAGAGGTAGTTTCAGAAATTTAATCAAGTTAGGCTTAGGATTTCGAAACGAAATCAAGTATTCTCAGTTGATTGTTAGTGTGTACAGTCTATGATTCTGTTTTTAACCGATTTAGGGCACTAAAACATCTCTAGAATGAATTTCTCTCCTCTGATTTTAGATCTTAATCGAAGATCCGTAAAGCAAAACAAACAAATTGTTAGTTCATATCTCTCTCTAATCGATTCTGCTTCTCTCTTTCTGAAACGGATTGATGAATCCGAAATTCACGGAGTAAATTTTGTGTGCTGAATTTGTGGAATTTGGAAATATTTGATGTAGTTATTGACGCAATTAAATACTGATTTTACAAGATATTCATATTCTTTCCATCTTTTAGATTATTCATAATTTGGTAATTTCTTTTGGATTTTTCTATTTTATCCTTCATTGATTCAATCTTCTCAATTACGTCTTTAACTTTTTTTAGAAATTTAAATTAATTAAGAGAAAGCTTATGTCTCGGATCGGTAAATTAATATTTTAATAACATATTTGGATTCCATAACAGATTTCATAATTTGTTAATGAAATACTAATCGCTTTTCATAACAGATTTTTTTTACTAAGGGCTCATCGGAAATTTTTAAAATCTTAATTAAATATAGAATTGAATATTTTAAATGTTTAACGACTAAATGGAGAATGTTTTTCTTTTAATTTTTTTATCAGGTTAAATTATTAAAAAAAAATATAACCAAATTCTTAATCGTTTGTGTTGTGTTTCAAGTTTTGTTTAGGGTTATGAGAACAATTCTAACACTTATATGGAGAAAAATGTAAATATGCGAACTTGTCGTCCATGAATTTAACAACAAAACTTTTCAAGGGTTGTTTTGAAAAAAATTTCAAACACTCACTTGAAATCCAAAAGACGTTTATAGATTATATAAATTTGGAGGCCAG

mRNA sequence

AAATAAAAAATTAAAGTTTCTCTCGGTTTTCAAATTTCAAAATCCCATTGATCTCTTTTGATCCTGAATCTTCACTCACGCCCATGGCCAAGAAGAACAACTCCCTTTCAATTTCAAATCCTGGATCCTCCTTGGGCTCCATGGACGACGTCATCAAGGTCTTCAACAAGTTCGACAAGAACGGCGACGGCAAGATTTCCGTTAACGAGCTCGGCGCTGCCCTCAGCGAACTTGGCGGCAAGATCTCCACCGACGAGATCCGCCGCATTATGTCTGAGATCGACAAGGACGGCGACGGATTCATCGACCTCGATGAGTTCGCCGAGTTTCATCAGGACGCCTCGTCTTCCAGCGGATGTAACAAAGATCTGCAGGACGCTTTCGATCTGTACGATATCGATAAGAACGGCTTAATCTCTGCTAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATTAGTTCCGTCGATGTCGACGGTGATGGACATGTAAATTTTGAGGAATTCAAGAAGATGATGACTCGCTCTTAGTCGTCTCCGGCAGTAGAGAGGTAGTTTCAGAAATTTAATCAAGTTAGGCTTAGGATTTCGAAACGAAATCAAGTATTCTCAGTTGATTGTTAGTGTGTACAGTCTATGATTCTGTTTTTAACCGATTTAGGGCACTAAAACATCTCTAGAATGAATTTCTCTCCTCTGATTTTAGATCTTAATCGAAGATCCGTAAAGCAAAACAAACAAATTGTTAGTTCATATCTCTCTCTAATCGATTCTGCTTCTCTCTTTCTGAAACGGATTGATGAATCCGAAATTCACGGAGTAAATTTTGTGTGCTGAATTTGTGGAATTTGGAAATATTTGATGTAGTTATTGACGCAATTAAATACTGATTTTACAAGATATTCATATTCTTTCCATCTTTTAGATTATTCATAATTTGGTAATTTCTTTTGGATTTTTCTATTTTATCCTTCATTGATTCAATCTTCTCAATTACGTCTTTAACTTTTTTTAGAAATTTAAATTAATTAAGAGAAAGCTTATGTCTCGGATCGGTAAATTAATATTTTAATAACATATTTGGATTCCATAACAGATTTCATAATTTGTTAATGAAATACTAATCGCTTTTCATAACAGATTTTTTTTACTAAGGGCTCATCGGAAATTTTTAAAATCTTAATTAAATATAGAATTGAATATTTTAAATGTTTAACGACTAAATGGAGAATGTTTTTCTTTTAATTTTTTTATCAGGTTAAATTATTAAAAAAAAATATAACCAAATTCTTAATCGTTTGTGTTGTGTTTCAAGTTTTGTTTAGGGTTATGAGAACAATTCTAACACTTATATGGAGAAAAATGTAAATATGCGAACTTGTCGTCCATGAATTTAACAACAAAACTTTTCAAGGGTTGTTTTGAAAAAAATTTCAAACACTCACTTGAAATCCAAAAGACGTTTATAGATTATATAAATTTGGAGGCCAG

Coding sequence (CDS)

ATGGCCAAGAAGAACAACTCCCTTTCAATTTCAAATCCTGGATCCTCCTTGGGCTCCATGGACGACGTCATCAAGGTCTTCAACAAGTTCGACAAGAACGGCGACGGCAAGATTTCCGTTAACGAGCTCGGCGCTGCCCTCAGCGAACTTGGCGGCAAGATCTCCACCGACGAGATCCGCCGCATTATGTCTGAGATCGACAAGGACGGCGACGGATTCATCGACCTCGATGAGTTCGCCGAGTTTCATCAGGACGCCTCGTCTTCCAGCGGATGTAACAAAGATCTGCAGGACGCTTTCGATCTGTACGATATCGATAAGAACGGCTTAATCTCTGCTAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATTAGTTCCGTCGATGTCGACGGTGATGGACATGTAAATTTTGAGGAATTCAAGAAGATGATGACTCGCTCTTAG

Protein sequence

MAKKNNSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS

Homology

[Close]

BLAST of Bhi01G001514 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 170.2 bits (430), Expect = 1.3e-42
Identity = 85/142 (59.86%), Postives = 107/142 (75.35%), Query Frame = 0


Query: 17  LGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMSEIDKDGDGFIDL 76
           LGSM+D+ KVF +FDKN DGKIS++EL   +  L    S +E + +M E D DG+GFIDL
Sbjct: 10  LGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDL 69

Query: 77  DEFAEFHQ--DASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCR 136
           DEF    Q  D SS++   +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC R
Sbjct: 70  DEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQR 129

Query: 137 MISSVDVDGDGHVNFEEFKKMM 157
           MI+ VD DGDG V+FEEFKKMM
Sbjct: 130 MINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Bhi01G001514 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 169.5 bits (428), Expect = 2.2e-42
Identity = 87/147 (59.18%), Postives = 107/147 (72.79%), Query Frame = 0


Query: 15  SSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMSEIDKDGDGFI 74
           S LGSMDD+ KVF +FDKNGDGKISV+EL   +  L    S +E   +M + D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 75  DLDEFAEFHQDASSSSGCNK----DLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLK 134
           DLDEF    Q      G N+    DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 135 DCCRMISSVDVDGDGHVNFEEFKKMMT 158
           DC +MIS VD+DGDG VNF+EFKKMM+
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of Bhi01G001514 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 159.5 bits (402), Expect = 2.3e-39
Identity = 82/160 (51.25%), Postives = 111/160 (69.38%), Query Frame = 0


Query: 1   MAKKNNSLSISNPGS-SLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MA  N   +   P +  + + +++ KVF++FD NGDGKISV ELG     +G   +  E+
Sbjct: 1   MASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETEL 60

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
            R++ E+D D DG+I+LDEF+   + +SS++    +++DAFDLYD DKNGLISA ELH V
Sbjct: 61  NRVLEEVDTDRDGYINLDEFSTLCRSSSSAA----EIRDAFDLYDQDKNGLISASELHQV 120

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           L RLG  CS++DC RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 121 LNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Bhi01G001514 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 159.5 bits (402), Expect = 2.3e-39
Identity = 82/160 (51.25%), Postives = 111/160 (69.38%), Query Frame = 0


Query: 1   MAKKNNSLSISNPGS-SLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MA  N   +   P +  + + +++ KVF++FD NGDGKISV ELG     +G   +  E+
Sbjct: 1   MASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETEL 60

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
            R++ E+D D DG+I+LDEF+   + +SS++    +++DAFDLYD DKNGLISA ELH V
Sbjct: 61  NRVLEEVDTDRDGYINLDEFSTLCRSSSSAA----EIRDAFDLYDQDKNGLISASELHQV 120

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           L RLG  CS++DC RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 121 LNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Bhi01G001514 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 151.4 bits (381), Expect = 6.2e-37
Identity = 77/153 (50.33%), Postives = 109/153 (71.24%), Query Frame = 0


Query: 5   NNSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMS 64
           N +L  +N  ++  +  ++ KVF+KFD NGDGKISV+ELG     +G   + +E+ R++ 
Sbjct: 3   NTNLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLD 62

Query: 65  EIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKRLG 124
           EID D DGFI+ +EFA   + +SS+     ++++AFDLYD +KNGLIS+ E+H VL RLG
Sbjct: 63  EIDIDCDGFINQEEFATICRSSSSA----VEIREAFDLYDQNKNGLISSSEIHKVLNRLG 122

Query: 125 EKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMT 158
             CS++DC RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 123 MTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151

[Close]

BLAST of Bhi01G001514 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.8e-41
Identity = 85/142 (59.86%), Postives = 107/142 (75.35%), Query Frame = 0


Query: 17  LGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMSEIDKDGDGFIDL 76
           LGSM+D+ KVF +FDKN DGKIS++EL   +  L    S +E + +M E D DG+GFIDL
Sbjct: 10  LGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDL 69

Query: 77  DEFAEFHQ--DASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCR 136
           DEF    Q  D SS++   +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC R
Sbjct: 70  DEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQR 129

Query: 137 MISSVDVDGDGHVNFEEFKKMM 157
           MI+ VD DGDG V+FEEFKKMM
Sbjct: 130 MINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of Bhi01G001514 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 169.5 bits (428), Expect = 3.1e-41
Identity = 87/147 (59.18%), Postives = 107/147 (72.79%), Query Frame = 0


Query: 15  SSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMSEIDKDGDGFI 74
           S LGSMDD+ KVF +FDKNGDGKISV+EL   +  L    S +E   +M + D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 75  DLDEFAEFHQDASSSSGCNK----DLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLK 134
           DLDEF    Q      G N+    DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 135 DCCRMISSVDVDGDGHVNFEEFKKMMT 158
           DC +MIS VD+DGDG VNF+EFKKMM+
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of Bhi01G001514 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 3.2e-38
Identity = 82/160 (51.25%), Postives = 111/160 (69.38%), Query Frame = 0


Query: 1   MAKKNNSLSISNPGS-SLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MA  N   +   P +  + + +++ KVF++FD NGDGKISV ELG     +G   +  E+
Sbjct: 1   MASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETEL 60

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
            R++ E+D D DG+I+LDEF+   + +SS++    +++DAFDLYD DKNGLISA ELH V
Sbjct: 61  NRVLEEVDTDRDGYINLDEFSTLCRSSSSAA----EIRDAFDLYDQDKNGLISASELHQV 120

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           L RLG  CS++DC RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 121 LNRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of Bhi01G001514 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 1.8e-36
Identity = 84/150 (56.00%), Postives = 101/150 (67.33%), Query Frame = 0


Query: 11  SNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMSEIDKDG 70
           S P   L   ++V  VFN+FD NGDGKIS +EL   L  LG   S +EI RIM EID D 
Sbjct: 9   SKPSVYLQEPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDK 68

Query: 71  DGFIDLDEFAEF---HQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKC 130
           DGFI++ EFA F     D   SSG   +L++AF+LYD D NGLIS+ ELH +L RLGE+ 
Sbjct: 69  DGFINVQEFAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERY 128

Query: 131 SLKDCCRMISSVDVDGDGHVNFEEFKKMMT 158
           +  DC  MI SVD DGDG+V+FEEFKKMMT
Sbjct: 129 AEHDCVEMIKSVDSDGDGYVSFEEFKKMMT 158

[Close]

BLAST of Bhi01G001514 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 8.8e-36
Identity = 77/153 (50.33%), Postives = 109/153 (71.24%), Query Frame = 0


Query: 5   NNSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRIMS 64
           N +L  +N  ++  +  ++ KVF+KFD NGDGKISV+ELG     +G   + +E+ R++ 
Sbjct: 3   NTNLESTNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLD 62

Query: 65  EIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKRLG 124
           EID D DGFI+ +EFA   + +SS+     ++++AFDLYD +KNGLIS+ E+H VL RLG
Sbjct: 63  EIDIDCDGFINQEEFATICRSSSSA----VEIREAFDLYDQNKNGLISSSEIHKVLNRLG 122

Query: 125 EKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMT 158
             CS++DC RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 123 MTCSVEDCVRMIGHVDTDGDGNVNFEEFQKMMS 151

[Close]

BLAST of Bhi01G001514 vs. NCBI nr
Match: XP_038879983.1 (probable calcium-binding protein CML23 [Benincasa hispida])

HSP 1 Score: 315.5 bits (807), Expect = 2.7e-82
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MAKKNNSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIR 60
           MAKKNNSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIR
Sbjct: 1   MAKKNNSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIR 60

Query: 61  RIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVL 120
           RIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVL
Sbjct: 61  RIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVL 120

Query: 121 KRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           KRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 KRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 159

[Close]

BLAST of Bhi01G001514 vs. NCBI nr
Match: XP_022143269.1 (probable calcium-binding protein CML23 [Momordica charantia])

HSP 1 Score: 271.9 bits (694), Expect = 3.4e-69
Identity = 137/160 (85.62%), Postives = 148/160 (92.50%), Query Frame = 0


Query: 1   MAKKN-NSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MAKK+ +  S +N G +LGSMD+V KVFNKFDKNGDGKISVNELG AL ELGGK+S+DE+
Sbjct: 3   MAKKSVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEV 62

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
           RRIM+EIDKDGDGFIDLDEFAEFHQD S++ G NKDLQDAFDLYDIDKNGLISAKELHSV
Sbjct: 63  RRIMAEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of Bhi01G001514 vs. NCBI nr
Match: XP_004145834.1 (probable calcium-binding protein CML23 [Cucumis sativus])

HSP 1 Score: 265.4 bits (677), Expect = 3.2e-67
Identity = 138/157 (87.90%), Postives = 143/157 (91.08%), Query Frame = 0


Query: 4   KNNSLSISNPGSS-LGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRI 63
           KNNSLSIS PGSS LGSMD++I+VFNKFDKNGDGKISV EL AAL EL G ISTDEI RI
Sbjct: 3   KNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRI 62

Query: 64  MSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKR 123
           MSEIDKDGDGFIDLDEF +F    SSS+G NKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 63  MSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 122

Query: 124 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 123 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

[Close]

BLAST of Bhi01G001514 vs. NCBI nr
Match: XP_022948508.1 (probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 probable calcium-binding protein CML23 [Cucurbita pepo subsp. pepo] >KAG6607012.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036709.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 264.2 bits (674), Expect = 7.1e-67
Identity = 134/160 (83.75%), Postives = 146/160 (91.25%), Query Frame = 0


Query: 1   MAKKN-NSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MAK + ++ S  NPG +LGSMD+VI VF KFDKNGDGKIS+ EL AAL ELGGKIS DEI
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
           RRIMSEIDKDG+GFIDLDEFAEFHQ++SS+ G +KDLQDAFDLYD+DKNGLISAKELHSV
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of Bhi01G001514 vs. NCBI nr
Match: XP_008457051.1 (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0053141.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK01546.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 264.2 bits (674), Expect = 7.1e-67
Identity = 139/160 (86.88%), Postives = 144/160 (90.00%), Query Frame = 0


Query: 1   MAKKNNSLSISNPG-SSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MA  N SLSIS PG SSLGSMD+VI+VFNKFDKNGDGKISV+ELGAAL EL G IST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
            RIMSEIDKDGDGFIDLDEF +F    SSS+G NKDLQDAFDLYDIDKNGLISAKELHSV
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSV 120

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

[Close]

BLAST of Bhi01G001514 vs. ExPASy TrEMBL
Match: A0A6J1CNU4 (probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC111013180 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.6e-69
Identity = 137/160 (85.62%), Postives = 148/160 (92.50%), Query Frame = 0


Query: 1   MAKKN-NSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MAKK+ +  S +N G +LGSMD+V KVFNKFDKNGDGKISVNELG AL ELGGK+S+DE+
Sbjct: 3   MAKKSVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEV 62

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
           RRIM+EIDKDGDGFIDLDEFAEFHQD S++ G NKDLQDAFDLYDIDKNGLISAKELHSV
Sbjct: 63  RRIMAEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of Bhi01G001514 vs. ExPASy TrEMBL
Match: A0A0A0LCM1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.5e-67
Identity = 138/157 (87.90%), Postives = 143/157 (91.08%), Query Frame = 0


Query: 4   KNNSLSISNPGSS-LGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRI 63
           KNNSLSIS PGSS LGSMD++I+VFNKFDKNGDGKISV EL AAL EL G ISTDEI RI
Sbjct: 3   KNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRI 62

Query: 64  MSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKR 123
           MSEIDKDGDGFIDLDEF +F    SSS+G NKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 63  MSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 122

Query: 124 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 123 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

[Close]

BLAST of Bhi01G001514 vs. ExPASy TrEMBL
Match: A0A5A7UHX3 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001160 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.4e-67
Identity = 139/160 (86.88%), Postives = 144/160 (90.00%), Query Frame = 0


Query: 1   MAKKNNSLSISNPG-SSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MA  N SLSIS PG SSLGSMD+VI+VFNKFDKNGDGKISV+ELGAAL EL G IST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
            RIMSEIDKDGDGFIDLDEF +F    SSS+G NKDLQDAFDLYDIDKNGLISAKELHSV
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSV 120

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

[Close]

BLAST of Bhi01G001514 vs. ExPASy TrEMBL
Match: A0A6J1G9H5 (probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC111452166 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.4e-67
Identity = 134/160 (83.75%), Postives = 146/160 (91.25%), Query Frame = 0


Query: 1   MAKKN-NSLSISNPGSSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MAK + ++ S  NPG +LGSMD+VI VF KFDKNGDGKIS+ EL AAL ELGGKIS DEI
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
           RRIMSEIDKDG+GFIDLDEFAEFHQ++SS+ G +KDLQDAFDLYD+DKNGLISAKELHSV
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of Bhi01G001514 vs. ExPASy TrEMBL
Match: A0A1S3C5W9 (probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.4e-67
Identity = 139/160 (86.88%), Postives = 144/160 (90.00%), Query Frame = 0


Query: 1   MAKKNNSLSISNPG-SSLGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEI 60
           MA  N SLSIS PG SSLGSMD+VI+VFNKFDKNGDGKISV+ELGAAL EL G IST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  RRIMSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSV 120
            RIMSEIDKDGDGFIDLDEF +F    SSS+G NKDLQDAFDLYDIDKNGLISAKELHSV
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSV 120

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 160
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

The following BLAST results are available for this feature:

BLAST of Bhi01G001514 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G66400.1	1.3e-42	59.86	calmodulin like 23	[more]
AT5G37770.1	2.2e-42	59.18	EF hand calcium-binding protein family	[more]
AT1G18210.1	2.3e-39	51.25	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	2.3e-39	51.25	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	6.2e-37	50.33	EF hand calcium-binding protein family	[more]

BLAST of Bhi01G001514 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C8Y1	1.8e-41	59.86	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	3.1e-41	59.18	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q9LE22	3.2e-38	51.25	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q9M7R0	1.8e-36	56.00	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q9C9U8	8.8e-36	50.33	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ...	[more]

BLAST of Bhi01G001514 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879983.1	2.7e-82	100.00	probable calcium-binding protein CML23 [Benincasa hispida]	[more]
XP_022143269.1	3.4e-69	85.63	probable calcium-binding protein CML23 [Momordica charantia]	[more]
XP_004145834.1	3.2e-67	87.90	probable calcium-binding protein CML23 [Cucumis sativus]	[more]
XP_022948508.1	7.1e-67	83.75	probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 prob...	[more]
XP_008457051.1	7.1e-67	86.88	PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0053141.1 p...	[more]

BLAST of Bhi01G001514 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNU4	1.6e-69	85.63	probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0LCM1	1.5e-67	87.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1	[more]
A0A5A7UHX3	3.4e-67	86.88	Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1G9H5	3.4e-67	83.75	probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A1S3C5W9	3.4e-67	86.88	probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 58..86 e-value: 8.1E-5 score: 32.0 coord: 22..50 e-value: 6.0E-6 score: 35.8 coord: 95..123 e-value: 3.2E-5 score: 33.4 coord: 131..159 e-value: 2.1E-4 score: 30.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 25..82 e-value: 7.8E-15 score: 55.1 coord: 94..156 e-value: 1.0E-11 score: 45.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 54..89 score: 14.06708
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..159 score: 13.174425
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 18..53 score: 16.103443
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 91..126 score: 14.094975
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 22..84 e-value: 1.65755E-18 score: 72.5805
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 95..157 e-value: 1.25176E-18 score: 72.9657
None	No IPR available	GENE3D	1.10.238.10		coord: 89..159 e-value: 8.6E-23 score: 82.4
None	No IPR available	GENE3D	1.10.238.10		coord: 2..88 e-value: 5.2E-22 score: 80.7
None	No IPR available	PANTHER	PTHR10891:SF794	CALCIUM-BINDING PROTEIN CML23-RELATED	coord: 15..158
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..158
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 67..79
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 31..43
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 104..116
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 20..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M001514	Bhi01M001514	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding