Bhi01G000130 (gene) Wax gourd (B227) v1

Overview
NameBhi01G000130
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr1: 3442961 .. 3444103 (+)
RNA-Seq ExpressionBhi01G000130
SyntenyBhi01G000130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCTGTAGGAAACTCAAATCAATGGCAGCCATATGCTTACAAAGACTGTTCTTTAGAGATTTGCAGCATATATTGCCCTCAGTGGTGTTACATCATCTTTCCACCACCTCCACCTTTTGGATATGGCAGCAATGGAGATTCTGGCACTGATTTCTCTCCTCTTATCATCGCCATCATTGGTATTTTGGCTAGCGCCTTCATTTTGGTAAGTTACTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTTCCACCTCCAACGCCGCCATGGAAATGGAAGATGAAGAAAATCTTTCCCAAATCCGCCATGAGAATCATCTCCAAGCTCCTCCTCCTCCTCCAGGTTTGGATGAGGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGAGATGGGTTAGTTGAAGGCACTGATTGCTCAGTTTGTTTGAGTGAGTTTGAAGAAAATGAGAGTTTAAGATTGCTCCCAAAGTGTAGCCATGCCTTTCATCTTCCTTGCATTGATACATGGCTTAAATCTCACTCAACTTGCCCTCTTTGCCGCTCCAACATTTCACCAACAAATCTTTTCCCACCCCCAACTCAAGAAATTCAAACAACACAACATTTTGTTTCATCTGATTTTCTGTATCAGCATCAGCACAGAACCAATGACACAGTAGTTGTTGTAGTGGTTCAAGATTTGCAGGGTTCAACAGTAGTGAGGCAAGAAATGGCTGATTCTAGGCTTGACAATGATTATGCTTCTTCCAAGAATCAAAGGGAAGATTTTGCATCAGATAGTCAAAATTCAGAGGCAAGAGACAGAATGAATCAAGTTAGACAGGAATATGATGTTCTGGATGGTGTTGTTCATCCATTTAGAAGGTCGGTTTCCTTGAATTCTTTATCATGGGAAGGGCAGGTTTCTGTTGCTGATATATTGAGAGTCAGTCAAGATAGTGAGGAAGAGGTAGAGGAAGATGAATTACAGCAAATGGGGATTGGATCTTCAGAAGTATTTGTTCAAGAGCATTCCAATTACAATCACAAACCTGGGGTTTCCAACTCGGGAATGAATAGATCTATTTCAACAGGGAAATTGGGTTTTACAAACTATGGGAAAGGAAAGAGTTGCATCAATCCCAGTTGA

mRNA sequence

ATGGGTTCTGTAGGAAACTCAAATCAATGGCAGCCATATGCTTACAAAGACTGTTCTTTAGAGATTTGCAGCATATATTGCCCTCAGTGGTGTTACATCATCTTTCCACCACCTCCACCTTTTGGATATGGCAGCAATGGAGATTCTGGCACTGATTTCTCTCCTCTTATCATCGCCATCATTGGTATTTTGGCTAGCGCCTTCATTTTGGTAAGTTACTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTTCCACCTCCAACGCCGCCATGGAAATGGAAGATGAAGAAAATCTTTCCCAAATCCGCCATGAGAATCATCTCCAAGCTCCTCCTCCTCCTCCAGGTTTGGATGAGGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGAGATGGGTTAGTTGAAGGCACTGATTGCTCAGTTTGTTTGAGTGAGTTTGAAGAAAATGAGAGTTTAAGATTGCTCCCAAAGTGTAGCCATGCCTTTCATCTTCCTTGCATTGATACATGGCTTAAATCTCACTCAACTTGCCCTCTTTGCCGCTCCAACATTTCACCAACAAATCTTTTCCCACCCCCAACTCAAGAAATTCAAACAACACAACATTTTGTTTCATCTGATTTTCTGTATCAGCATCAGCACAGAACCAATGACACAGTAGTTGTTGTAGTGGTTCAAGATTTGCAGGGTTCAACAGTAGTGAGGCAAGAAATGGCTGATTCTAGGCTTGACAATGATTATGCTTCTTCCAAGAATCAAAGGGAAGATTTTGCATCAGATAGTCAAAATTCAGAGGCAAGAGACAGAATGAATCAAGTTAGACAGGAATATGATGTTCTGGATGGTGTTGTTCATCCATTTAGAAGGTCGGTTTCCTTGAATTCTTTATCATGGGAAGGGCAGGTTTCTGTTGCTGATATATTGAGAGTCAGTCAAGATAGTGAGGAAGAGGTAGAGGAAGATGAATTACAGCAAATGGGGATTGGATCTTCAGAAGTATTTGTTCAAGAGCATTCCAATTACAATCACAAACCTGGGGTTTCCAACTCGGGAATGAATAGATCTATTTCAACAGGGAAATTGGGTTTTACAAACTATGGGAAAGGAAAGAGTTGCATCAATCCCAGTTGA

Coding sequence (CDS)

ATGGGTTCTGTAGGAAACTCAAATCAATGGCAGCCATATGCTTACAAAGACTGTTCTTTAGAGATTTGCAGCATATATTGCCCTCAGTGGTGTTACATCATCTTTCCACCACCTCCACCTTTTGGATATGGCAGCAATGGAGATTCTGGCACTGATTTCTCTCCTCTTATCATCGCCATCATTGGTATTTTGGCTAGCGCCTTCATTTTGGTAAGTTACTACACCATCATCTCCAAGTACTGCAGGAACAGAGCTTCCACCTCCAACGCCGCCATGGAAATGGAAGATGAAGAAAATCTTTCCCAAATCCGCCATGAGAATCATCTCCAAGCTCCTCCTCCTCCTCCAGGTTTGGATGAGGCTCTCATCAAGTCCATCACAGTCTGCAAGTACAAAAGAGGAGATGGGTTAGTTGAAGGCACTGATTGCTCAGTTTGTTTGAGTGAGTTTGAAGAAAATGAGAGTTTAAGATTGCTCCCAAAGTGTAGCCATGCCTTTCATCTTCCTTGCATTGATACATGGCTTAAATCTCACTCAACTTGCCCTCTTTGCCGCTCCAACATTTCACCAACAAATCTTTTCCCACCCCCAACTCAAGAAATTCAAACAACACAACATTTTGTTTCATCTGATTTTCTGTATCAGCATCAGCACAGAACCAATGACACAGTAGTTGTTGTAGTGGTTCAAGATTTGCAGGGTTCAACAGTAGTGAGGCAAGAAATGGCTGATTCTAGGCTTGACAATGATTATGCTTCTTCCAAGAATCAAAGGGAAGATTTTGCATCAGATAGTCAAAATTCAGAGGCAAGAGACAGAATGAATCAAGTTAGACAGGAATATGATGTTCTGGATGGTGTTGTTCATCCATTTAGAAGGTCGGTTTCCTTGAATTCTTTATCATGGGAAGGGCAGGTTTCTGTTGCTGATATATTGAGAGTCAGTCAAGATAGTGAGGAAGAGGTAGAGGAAGATGAATTACAGCAAATGGGGATTGGATCTTCAGAAGTATTTGTTCAAGAGCATTCCAATTACAATCACAAACCTGGGGTTTCCAACTCGGGAATGAATAGATCTATTTCAACAGGGAAATTGGGTTTTACAAACTATGGGAAAGGAAAGAGTTGCATCAATCCCAGTTGA

Protein sequence

MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAIIGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHSTCPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNSLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRSISTGKLGFTNYGKGKSCINPS
Homology
BLAST of Bhi01G000130 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 255.8 bits (652), Expect = 5.6e-68
Identity = 166/391 (42.46%), Postives = 221/391 (56.52%), Query Frame = 0

Query: 6   NSNQWQPY-AYKDCSLEICSIYCPQWCYIIFPPPPP--FGYGSNGDSGTDFSPLIIAIIG 65
           N N W PY +Y DCS  IC+IYCPQWCY+IFPPPPP  F    +  S + FSPL+IA+IG
Sbjct: 4   NPNPWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIG 63

Query: 66  ILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDEAL 125
           IL SA ILVSYYT+ISKYC     TS++  E  +  +  +    +  +      GL+E++
Sbjct: 64  ILTSALILVSYYTLISKYCHRHHQTSSS--ETLNLNHNGEGFFSSTQRISTNGDGLNESM 123

Query: 126 IKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHSTCP 185
           IKSITV KYK GDG V+G+DCSVCLSEFEENESLRLLPKC+HAFHLPCIDTWLKSHS CP
Sbjct: 124 IKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCP 183

Query: 186 LCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQEM 245
           LCR+ ++  N    PT  +                      V VVV      S    Q  
Sbjct: 184 LCRAFVTGVN---NPTASV-------------------GQNVSVVVANQ---SNSAHQTG 243

Query: 246 ADSRLDNDYASSKNQREDFAS---------DSQNSEARDRMNQVRQEYDVLDGVVHPFRR 305
           + S ++ + A  ++Q  DF S          S+NS+AR  +    +  +  D    P RR
Sbjct: 244 SVSEINLNLAGYESQTGDFDSVVVIEDLEIGSRNSDARSELQLPEERRETKDEDSLPIRR 303

Query: 306 SVSLNSLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEH---------SN 365
           SVSLNS      VS+AD+LR       E+E++E +  G+G+S+   +           + 
Sbjct: 304 SVSLNS---GVVVSIADVLR-------EIEDEEGESGGVGTSQRREEGEDGDGKTIPPTE 357

Query: 366 YNHKPGVSNSGMNRSISTGKLGFTNYGKGKS 376
            N + G  +    RS+STG+  F+ Y +G++
Sbjct: 364 ANQRSGGVSGFFVRSLSTGRFIFSRYDRGRN 357

BLAST of Bhi01G000130 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 251.9 bits (642), Expect = 8.1e-67
Identity = 170/380 (44.74%), Postives = 210/380 (55.26%), Query Frame = 0

Query: 1   MGSVGNSNQWQPY--AYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGD-SGTDFSPLI 60
           MGS GN N W     +Y+DCS  +CS+YCPQWCY+IFPPPP F      D S +DFSPL+
Sbjct: 1   MGSTGNPNPWGTTYDSYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLL 60

Query: 61  IAIIGILASAFILVSYYTIISKYC----RNRASTSNAAMEMEDEENLSQIRHENHLQAPP 120
           IA+IGILASAFILVSYYT+ISKYC     N +STS AA+     +   Q  + N+     
Sbjct: 61  IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGAT 120

Query: 121 PP---------PGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSH 180
            P          GLDE+LIKSITV KY++ DG VE +DCSVCLSEF+ENESLRLLPKC+H
Sbjct: 121 NPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNH 180

Query: 181 AFHLPCIDTWLKSHSTCPLCRSNI---SPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTN 240
           AFH+PCIDTWLKSHS CPLCR+ I   S   +     Q+I T  + +S          T 
Sbjct: 181 AFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSIS----------TG 240

Query: 241 DTVVVVVVQDLQGSTVVRQEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEY 300
           D  VVVV  DL+ S                  S+N+  +  S  +  E +D         
Sbjct: 241 DDSVVVVNLDLENS-----------------RSRNETVNEGSTPKPPEMQDSR------- 300

Query: 301 DVLDGVVHPFRRSVSLNSLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQE 360
              DG     RRS SLN  S  G VS+ADILR       E+E+DE +  G+G+S    + 
Sbjct: 301 ---DG---EERRSASLN--SGGGVVSIADILR-------EIEDDE-ESAGVGTSRWVEEG 330

Query: 361 HSNYNHKPGVSNSGMNRSIS 362
                  P  S +     IS
Sbjct: 361 EGEKTPPPSGSAANQTNGIS 330

BLAST of Bhi01G000130 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.8e-42
Identity = 96/224 (42.86%), Postives = 123/224 (54.91%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAY-KDCSLEICSIYCPQWCYIIFPPPPPFGY----GSNGDSGTDFSP 60
           M S  N + W  Y   +DC+  +CS +CPQWC  I   PPP  Y         S  + SP
Sbjct: 1   MESDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSP 60

Query: 61  LIIAIIGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPP- 120
           L+IAI GI A+AF+L +YYT++SKYC N  +T+ AA E    + +  +          P 
Sbjct: 61  LVIAIFGIFATAFLLAAYYTLVSKYCAN-DTTNEAASESGRSDIILDVNSPERGDQDDPF 120

Query: 121 -----PPGLDEALIKSITVCKYKRGDG--LVEGTDCSVCLSEFEENESLRLLPKCSHAFH 180
                  GLD+ LIK I   K K+      + GTDCS+CL EF E+ESLRLLPKC+H FH
Sbjct: 121 ALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFH 180

Query: 181 LPCIDTWLKSHSTCPLCRSN-ISPTNLFPPPTQEIQTTQHFVSS 211
           + CID WLKSHS CPLCR+  I PT   P     +     F S+
Sbjct: 181 VVCIDRWLKSHSNCPLCRAKIIVPTTQQPEHHVVVMNLDRFTSN 223

BLAST of Bhi01G000130 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-30
Identity = 122/400 (30.50%), Postives = 183/400 (45.75%), Query Frame = 0

Query: 17  DCSLEICSIYCPQWC------YIIFPPPPPFG----------------YGSNGDSGTDFS 76
           DCS  +C   CP  C      Y I P  PP+                 Y  + DS +   
Sbjct: 22  DCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 81

Query: 77  PLII-----AIIGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHL 136
            + I     A++ IL + F LV+ +   S    NR +      + ED + + +   ++  
Sbjct: 82  AISIITITGAVLAILLTGFFLVAKFFSDSV---NRVNQGTYQSDNEDNDTVMEEEFQDRE 141

Query: 137 QAPPP-----PPGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSH 196
           Q   P       GL +++I SIT+C YKRGDGL+E TDC VCL+EFEE+ESLRLLPKC+H
Sbjct: 142 QVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNH 201

Query: 197 AFHLPCIDTWLKSHSTCPLCRSNISPTNLFPPP-TQEIQTTQHFVSSDFLYQHQHRTNDT 256
           AFH+ CIDTWL SH+ CPLCR+ I+  ++  P  +  ++ T     S       H  ND 
Sbjct: 202 AFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGS-------HLENDG 261

Query: 257 VVVVVVQDLQGSTVVRQEMADSRLDNDYASSKNQ-------REDFASDSQNSEARDRMNQ 316
               V ++  G         ++R+D+D+  S +        R D   D   +E ++R+  
Sbjct: 262 ----VDEEDHGE-------IENRVDSDFKESDDSDIRIEIYRFDSDGDGSETETKERVRV 321

Query: 317 VRQEYDVLDGVVHPFRRSVSLNSLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSE 376
           +++  D   G         S+NSLS   +  V  +    +  E + EE        G  E
Sbjct: 322 LKECMDPNGG--------DSVNSLS-HTKTHVESVDFPGKSCENQSEEFTRHN---GEDE 381

BLAST of Bhi01G000130 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 2.3e-29
Identity = 76/179 (42.46%), Postives = 106/179 (59.22%), Query Frame = 0

Query: 29  QWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAIIG-ILASAFILVSYYTIISKYCRNRAS- 88
           ++C+  +PPPPP        S     P  I ++G I+   F++   Y  I++  R  A+ 
Sbjct: 19  RFCFRQYPPPPPPPPPPRELS--LLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAAS 78

Query: 89  -TSNAAMEMEDEENLSQIRHEN-HLQAPPPPPGLDEALIKSITVCKYKRGDGLVEGTDCS 148
            T     + EDE+   +    + H     P  GL  + I SITV  +K+G+G+++GT+CS
Sbjct: 79  VTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIIDGTECS 138

Query: 149 VCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHSTCPLCRSNISPTNLFPPPTQEIQT 204
           VCL+EFEE+ESLRLLPKCSHAFHL CIDTWL SH  CPLCR+ +    +  PP QE +T
Sbjct: 139 VCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPV--LLITEPPHQETET 193

BLAST of Bhi01G000130 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 7.9e-67
Identity = 166/391 (42.46%), Postives = 221/391 (56.52%), Query Frame = 0

Query: 6   NSNQWQPY-AYKDCSLEICSIYCPQWCYIIFPPPPP--FGYGSNGDSGTDFSPLIIAIIG 65
           N N W PY +Y DCS  IC+IYCPQWCY+IFPPPPP  F    +  S + FSPL+IA+IG
Sbjct: 4   NPNPWSPYDSYNDCSQGICNIYCPQWCYLIFPPPPPSFFLDDDSSSSSSSFSPLLIALIG 63

Query: 66  ILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDEAL 125
           IL SA ILVSYYT+ISKYC     TS++  E  +  +  +    +  +      GL+E++
Sbjct: 64  ILTSALILVSYYTLISKYCHRHHQTSSS--ETLNLNHNGEGFFSSTQRISTNGDGLNESM 123

Query: 126 IKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHSTCP 185
           IKSITV KYK GDG V+G+DCSVCLSEFEENESLRLLPKC+HAFHLPCIDTWLKSHS CP
Sbjct: 124 IKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCP 183

Query: 186 LCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQEM 245
           LCR+ ++  N    PT  +                      V VVV      S    Q  
Sbjct: 184 LCRAFVTGVN---NPTASV-------------------GQNVSVVVANQ---SNSAHQTG 243

Query: 246 ADSRLDNDYASSKNQREDFAS---------DSQNSEARDRMNQVRQEYDVLDGVVHPFRR 305
           + S ++ + A  ++Q  DF S          S+NS+AR  +    +  +  D    P RR
Sbjct: 244 SVSEINLNLAGYESQTGDFDSVVVIEDLEIGSRNSDARSELQLPEERRETKDEDSLPIRR 303

Query: 306 SVSLNSLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEH---------SN 365
           SVSLNS      VS+AD+LR       E+E++E +  G+G+S+   +           + 
Sbjct: 304 SVSLNS---GVVVSIADVLR-------EIEDEEGESGGVGTSQRREEGEDGDGKTIPPTE 357

Query: 366 YNHKPGVSNSGMNRSISTGKLGFTNYGKGKS 376
            N + G  +    RS+STG+  F+ Y +G++
Sbjct: 364 ANQRSGGVSGFFVRSLSTGRFIFSRYDRGRN 357

BLAST of Bhi01G000130 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 251.9 bits (642), Expect = 1.1e-65
Identity = 170/380 (44.74%), Postives = 210/380 (55.26%), Query Frame = 0

Query: 1   MGSVGNSNQWQPY--AYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGD-SGTDFSPLI 60
           MGS GN N W     +Y+DCS  +CS+YCPQWCY+IFPPPP F      D S +DFSPL+
Sbjct: 1   MGSTGNPNPWGTTYDSYRDCSQGVCSVYCPQWCYVIFPPPPSFYLDDEDDSSSSDFSPLL 60

Query: 61  IAIIGILASAFILVSYYTIISKYC----RNRASTSNAAMEMEDEENLSQIRHENHLQAPP 120
           IA+IGILASAFILVSYYT+ISKYC     N +STS AA+     +   Q  + N+     
Sbjct: 61  IALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGAT 120

Query: 121 PP---------PGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSH 180
            P          GLDE+LIKSITV KY++ DG VE +DCSVCLSEF+ENESLRLLPKC+H
Sbjct: 121 NPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNH 180

Query: 181 AFHLPCIDTWLKSHSTCPLCRSNI---SPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTN 240
           AFH+PCIDTWLKSHS CPLCR+ I   S   +     Q+I T  + +S          T 
Sbjct: 181 AFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSIS----------TG 240

Query: 241 DTVVVVVVQDLQGSTVVRQEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEY 300
           D  VVVV  DL+ S                  S+N+  +  S  +  E +D         
Sbjct: 241 DDSVVVVNLDLENS-----------------RSRNETVNEGSTPKPPEMQDSR------- 300

Query: 301 DVLDGVVHPFRRSVSLNSLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQE 360
              DG     RRS SLN  S  G VS+ADILR       E+E+DE +  G+G+S    + 
Sbjct: 301 ---DG---EERRSASLN--SGGGVVSIADILR-------EIEDDE-ESAGVGTSRWVEEG 330

Query: 361 HSNYNHKPGVSNSGMNRSIS 362
                  P  S +     IS
Sbjct: 361 EGEKTPPPSGSAANQTNGIS 330

BLAST of Bhi01G000130 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 221.9 bits (564), Expect = 1.3e-56
Identity = 148/344 (43.02%), Postives = 186/344 (54.07%), Query Frame = 0

Query: 10  WQPY-AYKDCSLEICSIYCPQWCYIIFPPPPPF---GYGSNGDSGTDFSPLIIAIIGILA 69
           W PY   +DCS  +CS+YCPQWCY IFPPPPPF   G  ++  SG  FSPL+IAIIG+LA
Sbjct: 8   WVPYEPTRDCSQGLCSMYCPQWCYFIFPPPPPFDVAGTSADDSSGPVFSPLVIAIIGVLA 67

Query: 70  SAFILVSYYTIISKYC------RNRASTSNAAMEMEDEENLSQIRHENHLQA-------P 129
           SAF+LVSYYT ISKYC      R R   S++          S  RH  H Q+        
Sbjct: 68  SAFLLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRH-GHGQSRSHESWNV 127

Query: 130 PPPPGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCID 189
            PP GLDE LI  ITVCKY+RGDG V  TDCSVCL EF + ESLRLLP+CSHAFH  CID
Sbjct: 128 SPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQCID 187

Query: 190 TWLKSHSTCPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDL 249
           TWLKSHS CPLCR+NI+   +     +           D          +T  VVVV D 
Sbjct: 188 TWLKSHSNCPLCRANITFVTVGLASPEPEGCAPGETGGD----------NTHEVVVVMDG 247

Query: 250 QGSTVVRQEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFR 309
             +    Q+ A SR     AS+ +   D    ++  E  +   ++R+E         P +
Sbjct: 248 LENLCEEQQEAVSR-----ASTADDDHDAKDVAEGMEEANGAAEIREEGS-------PPK 307

Query: 310 RSVSLNSLSWEGQVSVADILRVSQDSE-EEVEEDELQQMGIGSS 336
           R  S   L  + ++ +AD+L+ S + E     E  L   G G+S
Sbjct: 308 RGASSFDLHRDNRMCIADVLQESMEDELTAARESGLLAGGAGTS 328

BLAST of Bhi01G000130 vs. ExPASy Swiss-Prot
Match: P0C041 (Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 PE=3 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 2.6e-41
Identity = 96/224 (42.86%), Postives = 123/224 (54.91%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAY-KDCSLEICSIYCPQWCYIIFPPPPPFGY----GSNGDSGTDFSP 60
           M S  N + W  Y   +DC+  +CS +CPQWC  I   PPP  Y         S  + SP
Sbjct: 1   MESDPNPSAWNQYINPRDCTQGLCSTFCPQWCTYINFSPPPISYEQFLNDGVASNPNLSP 60

Query: 61  LIIAIIGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPP- 120
           L+IAI GI A+AF+L +YYT++SKYC N  +T+ AA E    + +  +          P 
Sbjct: 61  LVIAIFGIFATAFLLAAYYTLVSKYCAN-DTTNEAASESGRSDIILDVNSPERGDQDDPF 120

Query: 121 -----PPGLDEALIKSITVCKYKRGDG--LVEGTDCSVCLSEFEENESLRLLPKCSHAFH 180
                  GLD+ LIK I   K K+      + GTDCS+CL EF E+ESLRLLPKC+H FH
Sbjct: 121 ALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSICLGEFNEDESLRLLPKCNHTFH 180

Query: 181 LPCIDTWLKSHSTCPLCRSN-ISPTNLFPPPTQEIQTTQHFVSS 211
           + CID WLKSHS CPLCR+  I PT   P     +     F S+
Sbjct: 181 VVCIDRWLKSHSNCPLCRAKIIVPTTQQPEHHVVVMNLDRFTSN 223

BLAST of Bhi01G000130 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 1.7e-29
Identity = 122/400 (30.50%), Postives = 183/400 (45.75%), Query Frame = 0

Query: 17  DCSLEICSIYCPQWC------YIIFPPPPPFG----------------YGSNGDSGTDFS 76
           DCS  +C   CP  C      Y I P  PP+                 Y  + DS +   
Sbjct: 22  DCSNGVCDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLD 81

Query: 77  PLII-----AIIGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHL 136
            + I     A++ IL + F LV+ +   S    NR +      + ED + + +   ++  
Sbjct: 82  AISIITITGAVLAILLTGFFLVAKFFSDSV---NRVNQGTYQSDNEDNDTVMEEEFQDRE 141

Query: 137 QAPPP-----PPGLDEALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSH 196
           Q   P       GL +++I SIT+C YKRGDGL+E TDC VCL+EFEE+ESLRLLPKC+H
Sbjct: 142 QVDHPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNH 201

Query: 197 AFHLPCIDTWLKSHSTCPLCRSNISPTNLFPPP-TQEIQTTQHFVSSDFLYQHQHRTNDT 256
           AFH+ CIDTWL SH+ CPLCR+ I+  ++  P  +  ++ T     S       H  ND 
Sbjct: 202 AFHISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGS-------HLENDG 261

Query: 257 VVVVVVQDLQGSTVVRQEMADSRLDNDYASSKNQ-------REDFASDSQNSEARDRMNQ 316
               V ++  G         ++R+D+D+  S +        R D   D   +E ++R+  
Sbjct: 262 ----VDEEDHGE-------IENRVDSDFKESDDSDIRIEIYRFDSDGDGSETETKERVRV 321

Query: 317 VRQEYDVLDGVVHPFRRSVSLNSLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSE 376
           +++  D   G         S+NSLS   +  V  +    +  E + EE        G  E
Sbjct: 322 LKECMDPNGG--------DSVNSLS-HTKTHVESVDFPGKSCENQSEEFTRHN---GEDE 381

BLAST of Bhi01G000130 vs. NCBI nr
Match: XP_038901021.1 (RING-H2 finger protein ATL52-like [Benincasa hispida])

HSP 1 Score: 768.5 bits (1983), Expect = 2.8e-218
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDE 120
           IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQ 240
           CPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQ
Sbjct: 181 CPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQ 240

Query: 241 EMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNSL 300
           EMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNSL
Sbjct: 241 EMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNSL 300

Query: 301 SWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRSI 360
           SWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRSI
Sbjct: 301 SWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRSI 360

Query: 361 STGKLGFTNYGKGKSCINPS 381
           STGKLGFTNYGKGKSCINPS
Sbjct: 361 STGKLGFTNYGKGKSCINPS 380

BLAST of Bhi01G000130 vs. NCBI nr
Match: XP_008438300.1 (PREDICTED: RING-H2 finger protein ATL52 [Cucumis melo] >KAA0049120.1 RING-H2 finger protein ATL52 [Cucumis melo var. makuwa] >TYK17444.1 RING-H2 finger protein ATL52 [Cucumis melo var. makuwa])

HSP 1 Score: 675.2 bits (1741), Expect = 3.2e-190
Identity = 344/381 (90.29%), Postives = 354/381 (92.91%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPP-PPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSN AMEMEDEENLSQIRHEN LQAPP PPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENLSQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVR 240
           TCPLCRSNISPTNLF   TQEIQ TQHFVSS F YQHQHRTNDTV+VVVVQDL   TVVR
Sbjct: 181 TCPLCRSNISPTNLFSTATQEIQ-TQHFVSSAFQYQHQHRTNDTVLVVVVQDLDDLTVVR 240

Query: 241 QEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNS 300
           QE   SRL+ND ASSKNQRE +AS+SQNS  R+RMNQVRQEYDV+DGVV PFRRSVSLNS
Sbjct: 241 QETVVSRLENDDASSKNQREGYASESQNSGERERMNQVRQEYDVVDGVVQPFRRSVSLNS 300

Query: 301 LSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRS 360
           LSW+G VSVADILRVSQDSEEEVEEDELQQMGIGSS+VFVQE S+ NH+ GVSN GMNRS
Sbjct: 301 LSWQGPVSVADILRVSQDSEEEVEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNRS 360

Query: 361 ISTGKLGFTNYGKGKSCINPS 381
           ISTGKLGFTNYGKGKSCI PS
Sbjct: 361 ISTGKLGFTNYGKGKSCIIPS 380

BLAST of Bhi01G000130 vs. NCBI nr
Match: XP_004134368.1 (RING-H2 finger protein ATL51 [Cucumis sativus] >KGN56695.1 hypothetical protein Csa_010103 [Cucumis sativus])

HSP 1 Score: 675.2 bits (1741), Expect = 3.2e-190
Identity = 342/379 (90.24%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPP-PPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSN AMEMEDEEN+SQIRHEN LQAPP PPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVR 240
           TCPLCRSNISPTNLF  PTQEIQTTQHFVSS F YQHQHRTNDT+VVVVVQDL   TVVR
Sbjct: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240

Query: 241 QEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYD-VLDGVVHPFRRSVSLN 300
           QE  DSRL+ND ASSKNQRE  A++SQ S AR+RMNQVRQEYD V+DGVV PFRRSVSLN
Sbjct: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300

Query: 301 SLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNR 360
           SLSW+GQVSVADILRVSQDSEEE EEDELQQMGIGSS+VFVQE S+ NH+ GVSN GMNR
Sbjct: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCI 378
           SISTGKLGFTNYGKGKSCI
Sbjct: 361 SISTGKLGFTNYGKGKSCI 379

BLAST of Bhi01G000130 vs. NCBI nr
Match: XP_022147036.1 (RING-H2 finger protein ATL52-like [Momordica charantia])

HSP 1 Score: 608.6 bits (1568), Expect = 3.7e-170
Identity = 315/380 (82.89%), Postives = 341/380 (89.74%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYG+NGDSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDE 120
           IGILASAFILVSYYTIISKYCRNRA+T++AAMEM+DEENL+QIRHEN LQA  PP GLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRAATTSAAMEMDDEENLAQIRHENQLQA--PPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALIKSITVCKY RGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALIKSITVCKYIRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQ 240
           CPLCRSNISPTNLFPPPTQEIQ T+H  ++    Q+QHR+NDTVVVVVVQDLQ  T V Q
Sbjct: 181 CPLCRSNISPTNLFPPPTQEIQRTEHINAA--ALQYQHRSNDTVVVVVVQDLQDLT-VSQ 240

Query: 241 EMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNSL 300
           + A   ++ND ASSKNQR+ FAS SQ+SE RDRM QVR++ DV+  VV PFRRSVSL+S 
Sbjct: 241 QTAVLGIENDGASSKNQRQSFASVSQDSEPRDRMIQVRED-DVVGDVVQPFRRSVSLDSF 300

Query: 301 SWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRSI 360
           SW+GQVSVADILR SQDSEEEVEEDE+ QMGIGSS+VFVQEH   NHK GVSN GMNRS+
Sbjct: 301 SWQGQVSVADILRESQDSEEEVEEDEM-QMGIGSSKVFVQEHFQSNHKNGVSNLGMNRSV 360

Query: 361 STGKLGFTNYGKGKSCINPS 381
           STG+LGFTNY KGKSCI PS
Sbjct: 361 STGRLGFTNYEKGKSCIIPS 373

BLAST of Bhi01G000130 vs. NCBI nr
Match: XP_023540121.1 (RING-H2 finger protein ATL51-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594.0 bits (1530), Expect = 9.3e-166
Identity = 313/383 (81.72%), Postives = 332/383 (86.68%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG+N DSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGTNSDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDE 120
           IGILASAFILVSYYTIISKYCRNRA+TSN+ MEM+DEENLSQIRHEN LQAPPPP GLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRATTSNSNMEMDDEENLSQIRHENQLQAPPPPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQ 240
           CPLCRSNISP NLFPPPTQEIQ T+H  S +F  Q+QHR+NDTVVVVVVQDL   TV  Q
Sbjct: 181 CPLCRSNISPINLFPPPTQEIQRTEHLNSYNF--QYQHRSNDTVVVVVVQDLPDLTVSSQ 240

Query: 241 EMADSRLDNDYASSKNQREDFASDSQNSEARDRM-NQVRQEYDVLDGVVHPFRRSVSLNS 300
           E A   L+ND  SSKNQR+ F SD+Q+SE RD M +Q+RQ     D  V PFRRSVSLNS
Sbjct: 241 ETAVLGLENDGVSSKNQRQSFVSDNQDSEPRDIMIHQIRQ-----DDAVQPFRRSVSLNS 300

Query: 301 LSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRS 360
           LSW+GQVSVADILR SQDSEEEVEEDEL QMGIGSS+ FVQE  +      VSN  M+RS
Sbjct: 301 LSWQGQVSVADILRASQDSEEEVEEDEL-QMGIGSSKAFVQEDCHSR----VSNLEMHRS 360

Query: 361 ISTGKLGFTNYG--KGKSCINPS 381
           ISTGKLGFTNYG  KGKSCI PS
Sbjct: 361 ISTGKLGFTNYGNWKGKSCIIPS 371

BLAST of Bhi01G000130 vs. ExPASy TrEMBL
Match: A0A5D3D2D1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003080 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.5e-190
Identity = 344/381 (90.29%), Postives = 354/381 (92.91%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPP-PPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSN AMEMEDEENLSQIRHEN LQAPP PPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENLSQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVR 240
           TCPLCRSNISPTNLF   TQEIQ TQHFVSS F YQHQHRTNDTV+VVVVQDL   TVVR
Sbjct: 181 TCPLCRSNISPTNLFSTATQEIQ-TQHFVSSAFQYQHQHRTNDTVLVVVVQDLDDLTVVR 240

Query: 241 QEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNS 300
           QE   SRL+ND ASSKNQRE +AS+SQNS  R+RMNQVRQEYDV+DGVV PFRRSVSLNS
Sbjct: 241 QETVVSRLENDDASSKNQREGYASESQNSGERERMNQVRQEYDVVDGVVQPFRRSVSLNS 300

Query: 301 LSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRS 360
           LSW+G VSVADILRVSQDSEEEVEEDELQQMGIGSS+VFVQE S+ NH+ GVSN GMNRS
Sbjct: 301 LSWQGPVSVADILRVSQDSEEEVEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNRS 360

Query: 361 ISTGKLGFTNYGKGKSCINPS 381
           ISTGKLGFTNYGKGKSCI PS
Sbjct: 361 ISTGKLGFTNYGKGKSCIIPS 380

BLAST of Bhi01G000130 vs. ExPASy TrEMBL
Match: A0A0A0L405 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G129520 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.5e-190
Identity = 342/379 (90.24%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPP-PPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSN AMEMEDEEN+SQIRHEN LQAPP PPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENISQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVR 240
           TCPLCRSNISPTNLF  PTQEIQTTQHFVSS F YQHQHRTNDT+VVVVVQDL   TVVR
Sbjct: 181 TCPLCRSNISPTNLFSTPTQEIQTTQHFVSSAFQYQHQHRTNDTIVVVVVQDLDDLTVVR 240

Query: 241 QEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYD-VLDGVVHPFRRSVSLN 300
           QE  DSRL+ND ASSKNQRE  A++SQ S AR+RMNQVRQEYD V+DGVV PFRRSVSLN
Sbjct: 241 QETVDSRLENDDASSKNQREGCAAESQISGARERMNQVRQEYDVVVDGVVEPFRRSVSLN 300

Query: 301 SLSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNR 360
           SLSW+GQVSVADILRVSQDSEEE EEDELQQMGIGSS+VFVQE S+ NH+ GVSN GMNR
Sbjct: 301 SLSWQGQVSVADILRVSQDSEEEAEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNR 360

Query: 361 SISTGKLGFTNYGKGKSCI 378
           SISTGKLGFTNYGKGKSCI
Sbjct: 361 SISTGKLGFTNYGKGKSCI 379

BLAST of Bhi01G000130 vs. ExPASy TrEMBL
Match: A0A1S3AW58 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483448 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.5e-190
Identity = 344/381 (90.29%), Postives = 354/381 (92.91%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDS TDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSATDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPP-PPPGLD 120
           IGILASAFILVSYYTIISKYCRNRASTSN AMEMEDEENLSQIRHEN LQAPP PPPGLD
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRASTSNDAMEMEDEENLSQIRHENQLQAPPLPPPGLD 120

Query: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180
           EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS
Sbjct: 121 EALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHS 180

Query: 181 TCPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVR 240
           TCPLCRSNISPTNLF   TQEIQ TQHFVSS F YQHQHRTNDTV+VVVVQDL   TVVR
Sbjct: 181 TCPLCRSNISPTNLFSTATQEIQ-TQHFVSSAFQYQHQHRTNDTVLVVVVQDLDDLTVVR 240

Query: 241 QEMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNS 300
           QE   SRL+ND ASSKNQRE +AS+SQNS  R+RMNQVRQEYDV+DGVV PFRRSVSLNS
Sbjct: 241 QETVVSRLENDDASSKNQREGYASESQNSGERERMNQVRQEYDVVDGVVQPFRRSVSLNS 300

Query: 301 LSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRS 360
           LSW+G VSVADILRVSQDSEEEVEEDELQQMGIGSS+VFVQE S+ NH+ GVSN GMNRS
Sbjct: 301 LSWQGPVSVADILRVSQDSEEEVEEDELQQMGIGSSKVFVQEQSHSNHRTGVSNLGMNRS 360

Query: 361 ISTGKLGFTNYGKGKSCINPS 381
           ISTGKLGFTNYGKGKSCI PS
Sbjct: 361 ISTGKLGFTNYGKGKSCIIPS 380

BLAST of Bhi01G000130 vs. ExPASy TrEMBL
Match: A0A6J1D151 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016068 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.8e-170
Identity = 315/380 (82.89%), Postives = 341/380 (89.74%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYG+NGDSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGNNGDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDE 120
           IGILASAFILVSYYTIISKYCRNRA+T++AAMEM+DEENL+QIRHEN LQA  PP GLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRAATTSAAMEMDDEENLAQIRHENQLQA--PPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALIKSITVCKY RGDGLVEGTDCSVCLSEF+ENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALIKSITVCKYIRGDGLVEGTDCSVCLSEFQENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQ 240
           CPLCRSNISPTNLFPPPTQEIQ T+H  ++    Q+QHR+NDTVVVVVVQDLQ  T V Q
Sbjct: 181 CPLCRSNISPTNLFPPPTQEIQRTEHINAA--ALQYQHRSNDTVVVVVVQDLQDLT-VSQ 240

Query: 241 EMADSRLDNDYASSKNQREDFASDSQNSEARDRMNQVRQEYDVLDGVVHPFRRSVSLNSL 300
           + A   ++ND ASSKNQR+ FAS SQ+SE RDRM QVR++ DV+  VV PFRRSVSL+S 
Sbjct: 241 QTAVLGIENDGASSKNQRQSFASVSQDSEPRDRMIQVRED-DVVGDVVQPFRRSVSLDSF 300

Query: 301 SWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRSI 360
           SW+GQVSVADILR SQDSEEEVEEDE+ QMGIGSS+VFVQEH   NHK GVSN GMNRS+
Sbjct: 301 SWQGQVSVADILRESQDSEEEVEEDEM-QMGIGSSKVFVQEHFQSNHKNGVSNLGMNRSV 360

Query: 361 STGKLGFTNYGKGKSCINPS 381
           STG+LGFTNY KGKSCI PS
Sbjct: 361 STGRLGFTNYEKGKSCIIPS 373

BLAST of Bhi01G000130 vs. ExPASy TrEMBL
Match: A0A6J1G2W9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111450329 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.1e-164
Identity = 311/382 (81.41%), Postives = 329/382 (86.13%), Query Frame = 0

Query: 1   MGSVGNSNQWQPYAYKDCSLEICSIYCPQWCYIIFPPPPPFGYGSNGDSGTDFSPLIIAI 60
           MGSVGNSNQWQPYAY+DCSLEICSIYCPQWCYIIFPPPPPFGYG+N DSGTDFSPLIIAI
Sbjct: 1   MGSVGNSNQWQPYAYRDCSLEICSIYCPQWCYIIFPPPPPFGYGANSDSGTDFSPLIIAI 60

Query: 61  IGILASAFILVSYYTIISKYCRNRASTSNAAMEMEDEENLSQIRHENHLQAPPPPPGLDE 120
           IGILASAFILVSYYTIISKYCRNRA+TSN+ MEM+DEENLSQIRHEN LQAPPPP GLDE
Sbjct: 61  IGILASAFILVSYYTIISKYCRNRATTSNSNMEMDDEENLSQIRHENQLQAPPPPAGLDE 120

Query: 121 ALIKSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180
           ALI SITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST
Sbjct: 121 ALINSITVCKYKRGDGLVEGTDCSVCLSEFEENESLRLLPKCSHAFHLPCIDTWLKSHST 180

Query: 181 CPLCRSNISPTNLFPPPTQEIQTTQHFVSSDFLYQHQHRTNDTVVVVVVQDLQGSTVVRQ 240
           CPLCRSNISP NLFPPPTQEIQ T+H  S +F  Q+QHR+NDTVVVVVVQDL   TV  Q
Sbjct: 181 CPLCRSNISPINLFPPPTQEIQRTEHLNSYNF--QYQHRSNDTVVVVVVQDLPDLTVSSQ 240

Query: 241 EMADSRLDNDYASSKNQREDFASDSQNSEARDRM-NQVRQEYDVLDGVVHPFRRSVSLNS 300
           E A   L+ND  SSKNQR+ F SD+Q+SE RD M +Q RQ     D  V PFRRSVSLNS
Sbjct: 241 ETAVLGLENDGVSSKNQRQSFVSDNQDSEPRDIMIHQTRQ-----DDAVQPFRRSVSLNS 300

Query: 301 LSWEGQVSVADILRVSQDSEEEVEEDELQQMGIGSSEVFVQEHSNYNHKPGVSNSGMNRS 360
           LSW+GQVSVADILR SQDSEEEVEEDEL QMGIGSS+ FVQE  +      VSN  M+RS
Sbjct: 301 LSWQGQVSVADILRASQDSEEEVEEDEL-QMGIGSSKAFVQEDCHSR----VSNLEMHRS 360

Query: 361 ISTGKLGFTNYG--KGKSCINP 380
           ISTGKLGFTNYG  KGKSCI P
Sbjct: 361 ISTGKLGFTNYGNWKGKSCIIP 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G17600.15.6e-6842.46RING/U-box superfamily protein [more]
AT3G03550.18.1e-6744.74RING/U-box superfamily protein [more]
AT4G17905.11.8e-4242.86RING/U-box superfamily protein [more]
AT1G72220.11.2e-3030.50RING/U-box superfamily protein [more]
AT5G10380.12.3e-2942.46RING/U-box superfamily protein [more]
Match NameE-valueIdentityDescription
Q9LF647.9e-6742.46RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q9SRQ81.1e-6544.74RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Q7XLY81.3e-5643.02E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994... [more]
P0C0412.6e-4142.86Putative RING-H2 finger protein ATL53 OS=Arabidopsis thaliana OX=3702 GN=ATL53 P... [more]
Q8LFY81.7e-2930.50RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_038901021.12.8e-218100.00RING-H2 finger protein ATL52-like [Benincasa hispida][more]
XP_008438300.13.2e-19090.29PREDICTED: RING-H2 finger protein ATL52 [Cucumis melo] >KAA0049120.1 RING-H2 fin... [more]
XP_004134368.13.2e-19090.24RING-H2 finger protein ATL51 [Cucumis sativus] >KGN56695.1 hypothetical protein ... [more]
XP_022147036.13.7e-17082.89RING-H2 finger protein ATL52-like [Momordica charantia][more]
XP_023540121.19.3e-16681.72RING-H2 finger protein ATL51-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A5D3D2D11.5e-19090.29RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0L4051.5e-19090.24RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G129520 PE... [more]
A0A1S3AW581.5e-19090.29RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483448 PE=3 ... [more]
A0A6J1D1511.8e-17082.89RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101606... [more]
A0A6J1G2W91.1e-16481.41RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111450329... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 143..184
e-value: 2.0E-7
score: 40.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 142..185
e-value: 9.3E-14
score: 51.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 143..185
score: 12.99088
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 118..192
e-value: 6.9E-22
score: 78.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 243..273
NoneNo IPR availablePANTHERPTHR45676:SF95RING-H2 FINGER PROTEIN ATL53-RELATEDcoord: 3..370
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 142..185
e-value: 5.15193E-19
score: 77.7273
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 137..194
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 3..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000130Bhi01M000130mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane