Bhi01G000068 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G000068
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	EF-hand domain-containing protein
Location	chr1: 1850518 .. 1854853 (+)
RNA-Seq Expression	Bhi01G000068
Synteny	Bhi01G000068

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTCTTGGGCACAAAACCAATTGTGTAACTTGCATTGGAGGAAAGCAAAGGTCGCTTTATTCCAAGTTTCCTTTCGGACAGGAAAAGAAAATGTGTGGAGAAGCAGAATAGTTTCTGAAATTTATCCTTTTCAAATACAAAACCTCTGGAAATGATAAATTTCGCTATCGTTGCTGCTACGACGGATTCCTCAATCCTCCTTTGCCCGTAGCTCCGATTTCAATTACAGTCGGAGGCTGCTTTGCGAGCTCTGTGATCTTCAGTCACCGTCGGCGCCGTCTAGCATGGGACAGTCATTGGGGAGATTCGGAGGTAAATTGACCTAACTTGATTTTCTTATACTTCGTTTCGAATTCGTTTTCAGTTTTTTGTTTGGATCCGTCGATAAATCGATCAACGGATCGAGCGAAATAGCAAAATGGAGAATTCGTAGTCATAGAGGAGGTCATAGTGGTTTCTCCTTTGAAAGGAGATCGATCTTATTGATGAAATTCGATTATCCGTATTCATTAACTGATATCTTCACGGACTTGATAAATGAATTTATCGTTTTTGTTTCTTGTTTTGTTTGTTTGGTGTGTTAAATTAGCTGATATGCGCTGTCTCTGTGGATTTGTATCGTGTCTGCTTGCAATTGAGGGAGACATACACGTACAGGATGCTTAATAAGATTAGAAATCTTTTTGGAAATCGAGGGATGAGAAATTGATACATTTTTAATCTTAATATACTTGCAATTGCAGGCAAAGAATGGAGGGATGAGCAATTGAGGAAGATATCAAATAAAATATTTGATAAGTTTAAAAATCAATCACATAGGGATAAGCTGTCGTTTGAAGATCTGTACATTGCTACGCTACTTGTATACAAGTAAGAAGCTTAGTTATTTATTTATTTTTCCTTGCTACACTTTTCTAGCTTATTCATTCATTTATAAGATAGCCTGGTGGGACATCTTTCTTTATAATATGCACATGACCTACCCACTGATTTGTGGGGTTTGTGACTGTGAGAGTTCTTGCAAATTCTTGGCTAACTTAGGACGTAAGAACTGAAAGTTAGCTTTGCTAATATTTCCATTTCTTATTTCTCAATTTCCTTTTTCTTTTAAATTAAAAAACTAAAAACTAAAAAATAGATGTGTTTCTGGTTTTCTTTATTAAAAAAAAAATAGACGAGGGTTTGGTACCCTTTTTTAGTTTCTTTTTCCAAAATTTTAAAATTTTAAGAATTTTAAATTTTAAATTATTACATATAAATGATTTGTTTTAATTAAAGATTCAAATTTTAATGTTATGTAATAGTTGTATTATAAATTTTATAAGTAAAAAGATGAAATGATGCTTTTATAATTTCTTGAAAAAATTGAAAACATGAAGGAAAAAATTTGATTTAATTGTTTAATATTCCTAAAAGATTAGAAATAAGAAAGCGTTTATTTTACTCAAAAATTGGAAATAGGAAACACGAAACGAGAAATGTTATCAAACTGCTCTTAAATTTTAAAACTGGGCTTCAACTTTGATCCTTGGATATATCTGTTTAAAATCTCAAGCTCTTGGTGATTTGGCATTTATGACATTGGTTTAATACCGAACAAATACATGTAATGCAGTGATATTAATAAGTATATGCCCGGACCGCATCTTGATCCTCCCACGAAGGAACGGGTCAAAGAACTCATACAGGTACTTCGTTTTTTTAATTTTTTTTTTTTTATTGTTTTGAGAAAATAGAGGTACTTTGTTGATACAATAATATTGCAAAGGTTTAACAATGTAAGAAAGTGGCATCTGTTGTTGATTTGGAAACCTAAACTTGCTGGAAATGCATTCCATGGGCTCACCGTTCCTACTTATTTATTTGTTTATTTAATTGTGTCAAAGTAATCACCTACTTAACGATCCAATAAGTATCAAAGTAATAGATTATAATAAAAAATTACATCTCAATATTAACATATTGTTCAAACTTACATCTCAATAATCACCAATCTGCTAGCCCTTTTGTAAATATGTCCTGTTTGTTTTGTATCACAATGTAAACAACATATGGTAAACGATACTGGAAGAAGCGCAACATGTACTGATCCTCTCTTTCCTCGTCTGCTTCCTGTTTGGTTTCGGTAGAACTCAACGGAAGTCTAAAGAAATAACTGTTTTTTCTCTTCTTTTTGGAAACTAATATACTTTGACCTTCTAGATTTGAGTTTATTTGAGATGGGTGTCAAGGTTTCATAAAATGATTCTAAATTGTTATTAAGCTCATTTGAATATTAGTTGACCTGATAGCTAACGTAGTAGTTAAAATGTTATTGGACTTTGATGTTTAGGAATTTAAAATAATGGCAAAAGAAGTATCAACTTTATAGTATTCTCTTGTAGAATGCAAAATAATAATATAAATTTGGTACTTTTTTTGCCACTAAATGAAAAAAAAATGTGTATATAGATATATGTATGTATGTATTATTCTTTTGCTGACTGGATTGCTGACACGGCCATTCCTTCCCCTCTCTCATTACTCCCTTCTAATTTTCCAACCACCATTGTCGTTGCTGCCAACTGCCGTTCAGGATTGCATACTGCGAGTTCCAAATTTGGAACTTGTGTTGGTGAAATTGAGTGTAAAACCAGTAGGGGTGAGGTGAGCAGAAACGACAAATGGGTGGTGGGTGAATACCAAATGGCAAAACTAGTGGTGTTACTTTTAAAACTTTAGAAGCCAAAGAAACACCAAAATAATTATTAGAAAATATCGGATTCTTATCATTTTTACATTTATTCCATTGCGACCTCCCACCTCTTTCAGGCACTTATCTATTATCCTGAACGTGGAGTCGTCAATTAAATGACAATTATTAGGATAGTTCTGAAAATGAACTTTGCTTTGGTAAGTTAATGACGGATATTAATGCCTAGACCTTGTTCTCTACATATGAGGTCTAAATACTTGGCAATCTTGTTCTCCGCTTGGTGTTAATGAACTTGGCTGAAGCATGGCTATCAACTTGCCTGTGGTGCTTGTTGTTTTGTTTTCCTAATTCTCTTTGAGAGGCATTAGTAGTTTACTTTTGTGGCTTTTTTGAAATGTAGGCTAATCTTGCTCTTCCTGTTGCCATGCTTTCAAACACTTCCAACTAAAGTTTAACAATGCATCTTTTTCCAGTTATATATGCAAAGATTTTTTGTATGGATGACCCATTATCAGCACCCTAAATGATTAACCACCCCAAAATCAAATGAAAACTAACCGTTTGCTTATATCAAAGTGCACTTACGGTATGTTATATGGTATCACATTAGGGATGTCTCTTCTATTGCGAAATGGTCATGCCCATGTCTATCTCGATTTTCATTTTTCTCACATTTGTGTCATGGAATATTGTGGTGGAGTATAGAAAAATGAAGATTGCGATAGACATGGCTATCAGGATCGCATTAGGGATGTCTTTTATAGTGCAAAATGGTCAAGGCCATGTCATTTTTCTAACGCTTTTGATATCGAATATTGCGGTGGAGTGGAGAAAAATAAAGATCGTAGTAGACTAGGCCATCGGATTAGGGCTATGATGAAAGAGATGTCTCGAATGCTATATTATTTAAAAACACGGTAGTGTGCTGTGACATAAACATAAAACTAGTTTTTATTTGTTTTTAAGAGATGTGTTATTAATCATTTAGAAGGCTTGGAATGAGTCGTCCGTACAAAATCTCTATACTTGCAAACAACTACTTTATGGAAAACATGTAAAATCTGTGTGCTCTTGTTCCTACTCAACTTTGCATGTTGATGGTTCGTTTGTTGCATTACAGGACAGCGATAGGGACGAGGACCGAGAAATTGACCGTGAAGAATTTTTGGATTTCATACTGAGCTTGACATCTGACACGTTCGTATCAGTGAGCCAGAGATTGATCCTTACTTTGGTGGTAGCACCAACCGTCGCAGTTGTCACAAAGAAAAGTACTGAAGGCGTTCCAGGCCTTGGAAAATTTGTGCAAAAACTTCCCGCTTCAGCATATGCCTTCCTAGTCACCCTTGCTGCTCTCATGTTTCAAAACTCTCAGCAGCAGCTTCTCAAAGGATAGGTTCTGTCTCTGTTTCCTTCGTTTCATCTATATATTCCTTTGTTATTTGCATATATACGTGATTGTGAACTAATAACAATCCAACTTTGAATTTACCATTTGAAGCCTTGTTTATATTTTGTTTCTATGACTCTTCATCCCCCCCCCCCTCGTTCATGGAGGTCACACTCAAATTTGATTAGATCATGGCCGATAAATTTGCTTAAATTACATCTTTATTAATATGTCATTGGTATTTAAAA

mRNA sequence

ATGTGTCTTGGGCACAAAACCAATTGTGTAACTTGCATTGGAGGAAAGCAAAGGTCGCTTTATTCCAAGTTTCCTTTCGGACAGGAAAAGAAAATGTGTGGAGAAGCAGAATAGTTTCTGAAATTTATCCTTTTCAAATACAAAACCTCTGGAAATGATAAATTTCGCTATCGTTGCTGCTACGACGGATTCCTCAATCCTCCTTTGCCCGTAGCTCCGATTTCAATTACAGTCGGAGGCTGCTTTGCGAGCTCTGTGATCTTCAGTCACCGTCGGCGCCGTCTAGCATGGGACAGTCATTGGGGAGATTCGGAGGCAAAGAATGGAGGGATGAGCAATTGAGGAAGATATCAAATAAAATATTTGATAAGTTTAAAAATCAATCACATAGGGATAAGCTGTCGTTTGAAGATCTGTACATTGCTACGCTACTTGTATACAATGATATTAATAAGTATATGCCCGGACCGCATCTTGATCCTCCCACGAAGGAACGGGTCAAAGAACTCATACAGGACAGCGATAGGGACGAGGACCGAGAAATTGACCGTGAAGAATTTTTGGATTTCATACTGAGCTTGACATCTGACACGTTCGTATCAGTGAGCCAGAGATTGATCCTTACTTTGGTGGTAGCACCAACCGTCGCAGTTGTCACAAAGAAAAGTACTGAAGGCGTTCCAGGCCTTGGAAAATTTGTGCAAAAACTTCCCGCTTCAGCATATGCCTTCCTAGTCACCCTTGCTGCTCTCATGTTTCAAAACTCTCAGCAGCAGCTTCTCAAAGGATAGGTTCTGTCTCTGTTTCCTTCGTTTCATCTATATATTCCTTTGTTATTTGCATATATACGTGATTGTGAACTAATAACAATCCAACTTTGAATTTACCATTTGAAGCCTTGTTTATATTTTGTTTCTATGACTCTTCATCCCCCCCCCCCTCGTTCATGGAGGTCACACTCAAATTTGATTAGATCATGGCCGATAAATTTGCTTAAATTACATCTTTATTAATATGTCATTGGTATTTAAAA

Coding sequence (CDS)

ATGGGACAGTCATTGGGGAGATTCGGAGGCAAAGAATGGAGGGATGAGCAATTGAGGAAGATATCAAATAAAATATTTGATAAGTTTAAAAATCAATCACATAGGGATAAGCTGTCGTTTGAAGATCTGTACATTGCTACGCTACTTGTATACAATGATATTAATAAGTATATGCCCGGACCGCATCTTGATCCTCCCACGAAGGAACGGGTCAAAGAACTCATACAGGACAGCGATAGGGACGAGGACCGAGAAATTGACCGTGAAGAATTTTTGGATTTCATACTGAGCTTGACATCTGACACGTTCGTATCAGTGAGCCAGAGATTGATCCTTACTTTGGTGGTAGCACCAACCGTCGCAGTTGTCACAAAGAAAAGTACTGAAGGCGTTCCAGGCCTTGGAAAATTTGTGCAAAAACTTCCCGCTTCAGCATATGCCTTCCTAGTCACCCTTGCTGCTCTCATGTTTCAAAACTCTCAGCAGCAGCTTCTCAAAGGATAG

Protein sequence

MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPGPHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTVAVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLKG

Homology

[Close]

BLAST of Bhi01G000068 vs. TAIR 10
Match: AT1G64850.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 180.3 bits (456), Expect = 1.3e-45
Identity = 94/160 (58.75%), Postives = 123/160 (76.88%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ   +  GKEWR +Q++ I +++FD+FK Q+ R  L+FE+LYIA LLVYNDINK +PG
Sbjct: 1   MGQVFNKLRGKEWRHKQVQAICDRVFDRFKMQTGRANLTFEELYIAVLLVYNDINKRLPG 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
           PH DPP+K+ V  ++ D D + D E+DREEF+ FI  +T++TF  VSQ LI+ L+VAPTV
Sbjct: 61  PHFDPPSKDLVNSIMTDCDINLDGELDREEFVKFIEQITAETFNVVSQGLIIALIVAPTV 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNS 161
           A+ TKK+TEGVPG+GK VQKLP S YA LVTL A+M+ NS
Sbjct: 121 AIATKKATEGVPGVGKVVQKLPTSIYASLVTL-AVMWVNS 159

[Close]

BLAST of Bhi01G000068 vs. TAIR 10
Match: AT4G37445.1 (BEST Arabidopsis thaliana protein match is: Calcium-binding EF hand family protein (TAIR:AT1G64850.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 54.3 bits (129), Expect = 1.1e-07
Identity = 37/156 (23.72%), Postives = 79/156 (50.64%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MG S+G  G      + +  +   ++ +F  ++     +F+D ++A L ++N+ N  +PG
Sbjct: 1   MGSSMGFLGKGVPPTQMMNMVMGSLYKQFTQKAIN---NFDDFHVAVLDIFNNFNSALPG 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
            H D PT +++K         +D E  ++ F++F+      +   +    ++T +V+P  
Sbjct: 61  RHFDFPTPDQIKVCFARWKEAKDEEEKKKLFIEFMSKSVKPS--KLDDVTMITGIVSPPA 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALM 157
           A+  K++ E VP L K ++ +P   +   VT+ A++
Sbjct: 121 AMAAKRAGENVPQL-KLIKLIPDVIFVPTVTILAIV 150

[Close]

BLAST of Bhi01G000068 vs. NCBI nr
Match: XP_038876686.1 (uncharacterized protein LOC120069050 [Benincasa hispida])

HSP 1 Score: 320.1 bits (819), Expect = 1.1e-83
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG
Sbjct: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
           PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV
Sbjct: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLKG 168
           AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLKG
Sbjct: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLKG 167

[Close]

BLAST of Bhi01G000068 vs. NCBI nr
Match: XP_038892374.1 (uncharacterized protein LOC120081499 [Benincasa hispida])

HSP 1 Score: 237.7 bits (605), Expect = 7.5e-59
Identity = 131/171 (76.61%), Postives = 147/171 (85.96%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ+L  FGGKEWRD+QLRKI+++IFDKF+ Q+H DKL  EDLYIATLLVYN+INK +PG
Sbjct: 1   MGQALWTFGGKEWRDKQLRKITDRIFDKFQKQTHSDKLPAEDLYIATLLVYNEINKLIPG 60

Query: 61  PHLDPPT----KERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRL-ILTLV 120
           PH DPPT    KERVKELIQ SD + D+ IDR EFL FILSLTS+TF SVSQRL ILTLV
Sbjct: 61  PHRDPPTTERVKERVKELIQKSDMNGDQYIDRGEFLHFILSLTSETFKSVSQRLSILTLV 120

Query: 121 VAPTVAVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLK 167
           VAPTVAVVTKKSTE +PG+GK VQKLP SAYAFLVTLAAL+FQ SQQQLL+
Sbjct: 121 VAPTVAVVTKKSTEAIPGVGKLVQKLPVSAYAFLVTLAALIFQKSQQQLLE 171

[Close]

BLAST of Bhi01G000068 vs. NCBI nr
Match: KAA0049066.1 (uncharacterized protein E6C27_scaffold171G002950 [Cucumis melo var. makuwa])

HSP 1 Score: 233.8 bits (595), Expect = 1.1e-57
Identity = 119/166 (71.69%), Postives = 146/166 (87.95%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ+L RFGG++W+++QL  ISNKIFD+F    H++KLSFEDLYIATLLV+NDINKY+PG
Sbjct: 1   MGQALRRFGGQDWKNKQLEMISNKIFDRF----HKEKLSFEDLYIATLLVFNDINKYLPG 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
           PH+DPPTKERV EL++ SD +E++ IDR+EFL FI  LT ++  SVSQRLI+TLVVAPT+
Sbjct: 61  PHIDPPTKERVAELVEKSDENENQAIDRDEFLKFIKLLTFESIASVSQRLIITLVVAPTL 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLK 167
           AVVTK+STEG+PGLGK VQK+P+SAYA LVTLAAL+FQNS QQLLK
Sbjct: 121 AVVTKRSTEGIPGLGKMVQKVPSSAYALLVTLAALLFQNSNQQLLK 162

[Close]

BLAST of Bhi01G000068 vs. NCBI nr
Match: XP_008438214.1 (PREDICTED: uncharacterized protein LOC103483388 [Cucumis melo])

HSP 1 Score: 231.5 bits (589), Expect = 5.4e-57
Identity = 118/166 (71.08%), Postives = 146/166 (87.95%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ+L RF G++W+++QL  ISNKIFD+F    H++KLSFEDLYIATLLV+NDINKY+PG
Sbjct: 1   MGQALRRFRGQDWKNKQLEMISNKIFDRF----HKEKLSFEDLYIATLLVFNDINKYLPG 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
           PH+DPPTKERV EL++ SD++E++ IDR+EFL FI  LT ++  SVSQRLI+TLVVAPT+
Sbjct: 61  PHIDPPTKERVAELVEKSDKNENQAIDRDEFLKFIKLLTFESIASVSQRLIITLVVAPTL 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLK 167
           AVVTK+STEG+PGLGK VQK+P+SAYA LVTLAAL+FQNS QQLLK
Sbjct: 121 AVVTKRSTEGIPGLGKMVQKVPSSAYALLVTLAALLFQNSNQQLLK 162

[Close]

BLAST of Bhi01G000068 vs. NCBI nr
Match: XP_022146952.1 (uncharacterized protein LOC111016024 [Momordica charantia])

HSP 1 Score: 229.9 bits (585), Expect = 1.6e-56
Identity = 119/162 (73.46%), Postives = 140/162 (86.42%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ L + GGK+WRD Q+RKIS+KIF+K KN S +  LSFEDLYIATLLVYN+INK +PG
Sbjct: 54  MGQILDKIGGKQWRDAQMRKISDKIFNKIKNHSDKVNLSFEDLYIATLLVYNEINKRLPG 113

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
           PH DPP+KERV+ELI++SD + D+EIDREEF+ FIL LTSDT ++VSQ L+LTLVVAPTV
Sbjct: 114 PHHDPPSKERVRELIKESDLNMDQEIDREEFMKFILLLTSDTVITVSQGLVLTLVVAPTV 173

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQ 163
           AVVTKKSTEGVPG+GK VQKLPAS YA L+TLAAL FQN+ Q
Sbjct: 174 AVVTKKSTEGVPGVGKLVQKLPASVYASLITLAALSFQNAHQ 215

[Close]

BLAST of Bhi01G000068 vs. ExPASy TrEMBL
Match: A0A5A7U485 (EF-hand domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002950 PE=4 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 5.2e-58
Identity = 119/166 (71.69%), Postives = 146/166 (87.95%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ+L RFGG++W+++QL  ISNKIFD+F    H++KLSFEDLYIATLLV+NDINKY+PG
Sbjct: 1   MGQALRRFGGQDWKNKQLEMISNKIFDRF----HKEKLSFEDLYIATLLVFNDINKYLPG 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
           PH+DPPTKERV EL++ SD +E++ IDR+EFL FI  LT ++  SVSQRLI+TLVVAPT+
Sbjct: 61  PHIDPPTKERVAELVEKSDENENQAIDRDEFLKFIKLLTFESIASVSQRLIITLVVAPTL 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLK 167
           AVVTK+STEG+PGLGK VQK+P+SAYA LVTLAAL+FQNS QQLLK
Sbjct: 121 AVVTKRSTEGIPGLGKMVQKVPSSAYALLVTLAALLFQNSNQQLLK 162

[Close]

BLAST of Bhi01G000068 vs. ExPASy TrEMBL
Match: A0A1S3AVI1 (uncharacterized protein LOC103483388 OS=Cucumis melo OX=3656 GN=LOC103483388 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 2.6e-57
Identity = 118/166 (71.08%), Postives = 146/166 (87.95%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ+L RF G++W+++QL  ISNKIFD+F    H++KLSFEDLYIATLLV+NDINKY+PG
Sbjct: 1   MGQALRRFRGQDWKNKQLEMISNKIFDRF----HKEKLSFEDLYIATLLVFNDINKYLPG 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
           PH+DPPTKERV EL++ SD++E++ IDR+EFL FI  LT ++  SVSQRLI+TLVVAPT+
Sbjct: 61  PHIDPPTKERVAELVEKSDKNENQAIDRDEFLKFIKLLTFESIASVSQRLIITLVVAPTL 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLK 167
           AVVTK+STEG+PGLGK VQK+P+SAYA LVTLAAL+FQNS QQLLK
Sbjct: 121 AVVTKRSTEGIPGLGKMVQKVPSSAYALLVTLAALLFQNSNQQLLK 162

[Close]

BLAST of Bhi01G000068 vs. ExPASy TrEMBL
Match: A0A6J1D109 (uncharacterized protein LOC111016024 OS=Momordica charantia OX=3673 GN=LOC111016024 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 7.5e-57
Identity = 119/162 (73.46%), Postives = 140/162 (86.42%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ L + GGK+WRD Q+RKIS+KIF+K KN S +  LSFEDLYIATLLVYN+INK +PG
Sbjct: 54  MGQILDKIGGKQWRDAQMRKISDKIFNKIKNHSDKVNLSFEDLYIATLLVYNEINKRLPG 113

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
           PH DPP+KERV+ELI++SD + D+EIDREEF+ FIL LTSDT ++VSQ L+LTLVVAPTV
Sbjct: 114 PHHDPPSKERVRELIKESDLNMDQEIDREEFMKFILLLTSDTVITVSQGLVLTLVVAPTV 173

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQ 163
           AVVTKKSTEGVPG+GK VQKLPAS YA L+TLAAL FQN+ Q
Sbjct: 174 AVVTKKSTEGVPGVGKLVQKLPASVYASLITLAALSFQNAHQ 215

[Close]

BLAST of Bhi01G000068 vs. ExPASy TrEMBL
Match: A0A0A0L6I0 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G126970 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.9e-56
Identity = 117/166 (70.48%), Postives = 145/166 (87.35%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           MGQ+  R GGKEWR+ QL  ISNKIFD+F     +++LSFEDLYIATLLV+NDINKY+PG
Sbjct: 1   MGQAFRRLGGKEWRNTQLENISNKIFDRF----GKERLSFEDLYIATLLVFNDINKYLPG 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
            H+DPP K+RVKE+++  D++++  IDREEFL+F+  LTS++F+SVSQRLI+TLVVAPT+
Sbjct: 61  QHVDPPKKDRVKEVMEMCDKNQNYAIDREEFLEFVKQLTSESFISVSQRLIITLVVAPTL 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLK 167
           AVVTKKSTEGVPGLGK VQK+P+SAYA LVTLAAL+FQNS+QQLLK
Sbjct: 121 AVVTKKSTEGVPGLGKMVQKVPSSAYALLVTLAALLFQNSRQQLLK 162

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BLAST of Bhi01G000068 vs. ExPASy TrEMBL
Match: A0A6J1EC16 (uncharacterized protein LOC111431834 OS=Cucurbita moschata OX=3662 GN=LOC111431834 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 3.2e-55
Identity = 117/166 (70.48%), Postives = 145/166 (87.35%), Query Frame = 0


Query: 1   MGQSLGRFGGKEWRDEQLRKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYMPG 60
           M ++LGRFGGKEWRDEQLR IS++IFD   ++  +DKLSFEDLYIATLLVYNDINK +P 
Sbjct: 1   MRKALGRFGGKEWRDEQLRNISDRIFDMIGDK--KDKLSFEDLYIATLLVYNDINKRLPI 60

Query: 61  PHLDPPTKERVKELIQDSDRDEDREIDREEFLDFILSLTSDTFVSVSQRLILTLVVAPTV 120
            HLDPPT+ER +ELI+++D ++D++I R+EFL FILSLTSD F+S+S RLILTLV+APTV
Sbjct: 61  SHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTV 120

Query: 121 AVVTKKSTEGVPGLGKFVQKLPASAYAFLVTLAALMFQNSQQQLLK 167
           AVVTK+STEG+P +G  VQKLP+SAYAFLVTLAA +FQNSQ+ +LK
Sbjct: 121 AVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK 164

The following BLAST results are available for this feature:

BLAST of Bhi01G000068 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64850.1	1.3e-45	58.75	Calcium-binding EF hand family protein	[more]
AT4G37445.1	1.1e-07	23.72	BEST Arabidopsis thaliana protein match is: Calcium-binding EF hand family prote...	[more]

BLAST of Bhi01G000068 vs. TAIR 10


Match Name	E-value	Identity	Description

BLAST of Bhi01G000068 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876686.1	1.1e-83	100.00	uncharacterized protein LOC120069050 [Benincasa hispida]	[more]
XP_038892374.1	7.5e-59	76.61	uncharacterized protein LOC120081499 [Benincasa hispida]	[more]
KAA0049066.1	1.1e-57	71.69	uncharacterized protein E6C27_scaffold171G002950 [Cucumis melo var. makuwa]	[more]
XP_008438214.1	5.4e-57	71.08	PREDICTED: uncharacterized protein LOC103483388 [Cucumis melo]	[more]
XP_022146952.1	1.6e-56	73.46	uncharacterized protein LOC111016024 [Momordica charantia]	[more]

BLAST of Bhi01G000068 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U485	5.2e-58	71.69	EF-hand domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A1S3AVI1	2.6e-57	71.08	uncharacterized protein LOC103483388 OS=Cucumis melo OX=3656 GN=LOC103483388 PE=...	[more]
A0A6J1D109	7.5e-57	73.46	uncharacterized protein LOC111016024 OS=Momordica charantia OX=3673 GN=LOC111016...	[more]
A0A0A0L6I0	2.9e-56	70.48	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G126970 PE=...	[more]
A0A6J1EC16	3.2e-55	70.48	uncharacterized protein LOC111431834 OS=Cucurbita moschata OX=3662 GN=LOC1114318...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 11..110 e-value: 4.3E-7 score: 31.9
None	No IPR available	PANTHER	PTHR11639	S100 CALCIUM-BINDING PROTEIN	coord: 5..163
None	No IPR available	PANTHER	PTHR11639:SF133	CALCIUM-BINDING EF HAND PROTEIN	coord: 5..163
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 79..91
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 66..101 score: 12.198088
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 23..100

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M000068	Bhi01M000068	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0048306	calcium-dependent protein binding
molecular_function	GO:0005509	calcium ion binding