Chrysanthenum transcriptome database

GenBank blast output of UN81678


BLASTX 7.6.2

Query= UN81678 /QuerySize=1341
        (1340 letters)

Database: GenBank nr;
          20,571,509 sequences; 7,061,663,739 total letters
                                                                  Score    E
Sequences producing significant alignments:                       (bits) Value

gi|316936736|gb|ADU60362.1| glutamyl-tRNA reductase binding prot...    290   5e-076
gi|225443292|ref|XP_002274572.1| PREDICTED: uncharacterized prot...    289   1e-075
gi|298204792|emb|CBI25290.3| unnamed protein product [Vitis vini...    289   1e-075
gi|316936734|gb|ADU60361.1| glutamyl-tRNA reductase binding prot...    289   1e-075
gi|224113295|ref|XP_002316448.1| predicted protein [Populus tric...    278   2e-072
gi|357467765|ref|XP_003604167.1| hypothetical protein MTR_4g0062...    263   8e-068
gi|388518743|gb|AFK47433.1| unknown [Lotus japonicus]                  260   5e-067
gi|297830820|ref|XP_002883292.1| hypothetical protein ARALYDRAFT...    245   2e-062
gi|21593452|gb|AAM65419.1| unknown [Arabidopsis thaliana]              245   2e-062
gi|35187431|gb|AAQ84307.1| fiber protein Fb4 [Gossypium barbadense]    225   2e-056

>gi|316936736|gb|ADU60362.1| glutamyl-tRNA reductase binding protein [Nicotiana
        tabacum]

          Length = 320

 Score =  290 bits (742), Expect = 5e-076
 Identities = 158/283 (55%), Positives = 192/283 (67%), Gaps = 12/283 (4%)
 Frame = -2

Query: 1174 RCCVSSSVEVAAPPQSIIISSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXCVGV 995
            RC VS    V+ P QS    + N KP  AEV+RTIMELSSVGT               GV
Sbjct:   45 RCAVS---VVSEPIQS--EKTNNGKPYPAEVSRTIMELSSVGTLSTPTLDGWPLG--FGV 97

Query:  994 RFAVDPQDGSPIVSLNPNHHFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPPSNP 815
            RFAVDP  G+P++ LN                  VQ EQ  GLRT QCTI  +L  P + 
Sbjct:   98 RFAVDPH-GTPVLFLN---DATSKLSVNCKSSIHVQFEQ-YGLRTPQCTIQGTLEKPEDT 152

Query:  814 IHLNKLQSLWEKRFREKANSNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPLRDC 635
              L KL S+W+KRF  + + + + LL V+RVLQL++F +DG+W++S DY LANPDPLRD 
Sbjct:  153 TALKKLHSVWKKRFGHEVDEDHLFLLSVERVLQLEDFAEDGIWVTSSDYKLANPDPLRDF 212

Query:  634 AQRLVDGFNTDNREDVLRFCNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEVRIP 455
            A+R+++  NT NRED+LRFCNIYVD   QVSDAKM+W+DRLGFDV   SP+NDVFE RIP
Sbjct:  213 AERMIEEINTHNREDILRFCNIYVDLDFQVSDAKMLWVDRLGFDVRFRSPQNDVFEARIP 272

Query:  454 FPREVTNEKGAKSSFNGMAQQAWEVEKNYHALEFQKATQLNRI 326
            FPREVT+EKGAKSSFN M+Q AWEVEKN+HA EF+KA QL +I
Sbjct:  273 FPREVTDEKGAKSSFNCMSQLAWEVEKNFHAAEFEKAKQLKKI 315

>gi|225443292|ref|XP_002274572.1| PREDICTED: uncharacterized protein
        LOC100260424 [Vitis vinifera]

          Length = 324

 Score =  289 bits (739), Expect = 1e-075
 Identities = 155/283 (54%), Positives = 195/283 (68%), Gaps = 11/283 (3%)
 Frame = -2

Query: 1156 SVEVAAPPQSIIISSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXCVGVRFAVDP 977
            SV VA+ P  + +    NKP  AEV+RTIMELSSV T              VGVRFAVDP
Sbjct:   52 SVSVASEPAHLEL--VRNKPFPAEVSRTIMELSSVATLSALTQDGWPLG--VGVRFAVDP 107

Query:  976 QDGSPIVSLNPNH-HFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPPSNPIHLNK 800
            Q G+PIV LN +H HF             VQLEQC GLRT QCTI  SL  P + + L K
Sbjct:  108 Q-GTPIVCLNASHSHF----SIDRRSSLHVQLEQC-GLRTPQCTIQGSLDKPEDRMALKK 161

Query:  799 LQSLWEKRFREKANSNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPLRDCAQRLV 620
            L S+WE+RF ++ + N +H++ V+RVLQ+++F + G W++S DY  ANPDPLRD A++ V
Sbjct:  162 LHSIWERRFGKEVDENLLHVVSVERVLQMEDFQEVGTWVTSSDYRTANPDPLRDSAEKFV 221

Query:  619 DGFNTDNREDVLRFCNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEVRIPFPREV 440
            D  NT+N EDV RFCNIYVD   Q  +AK++W+DRLGFD+ + SP+ DVFEVRIPFPREV
Sbjct:  222 DEINTNNMEDVNRFCNIYVDLDFQALEAKLIWVDRLGFDMRISSPQKDVFEVRIPFPREV 281

Query:  439 TNEKGAKSSFNGMAQQAWEVEKNYHALEFQKATQLNRIASKLQ 311
            T+EKGAKSSFN M+Q AWEVEKNY+A +F+K  QL +I  + Q
Sbjct:  282 TDEKGAKSSFNSMSQLAWEVEKNYNAPDFKKVKQLKQITYRGQ 324

>gi|298204792|emb|CBI25290.3| unnamed protein product [Vitis vinifera]

          Length = 318

 Score =  289 bits (739), Expect = 1e-075
 Identities = 155/283 (54%), Positives = 195/283 (68%), Gaps = 11/283 (3%)
 Frame = -2

Query: 1156 SVEVAAPPQSIIISSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXCVGVRFAVDP 977
            SV VA+ P  + +    NKP  AEV+RTIMELSSV T              VGVRFAVDP
Sbjct:   46 SVSVASEPAHLEL--VRNKPFPAEVSRTIMELSSVATLSALTQDGWPLG--VGVRFAVDP 101

Query:  976 QDGSPIVSLNPNH-HFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPPSNPIHLNK 800
            Q G+PIV LN +H HF             VQLEQC GLRT QCTI  SL  P + + L K
Sbjct:  102 Q-GTPIVCLNASHSHF----SIDRRSSLHVQLEQC-GLRTPQCTIQGSLDKPEDRMALKK 155

Query:  799 LQSLWEKRFREKANSNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPLRDCAQRLV 620
            L S+WE+RF ++ + N +H++ V+RVLQ+++F + G W++S DY  ANPDPLRD A++ V
Sbjct:  156 LHSIWERRFGKEVDENLLHVVSVERVLQMEDFQEVGTWVTSSDYRTANPDPLRDSAEKFV 215

Query:  619 DGFNTDNREDVLRFCNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEVRIPFPREV 440
            D  NT+N EDV RFCNIYVD   Q  +AK++W+DRLGFD+ + SP+ DVFEVRIPFPREV
Sbjct:  216 DEINTNNMEDVNRFCNIYVDLDFQALEAKLIWVDRLGFDMRISSPQKDVFEVRIPFPREV 275

Query:  439 TNEKGAKSSFNGMAQQAWEVEKNYHALEFQKATQLNRIASKLQ 311
            T+EKGAKSSFN M+Q AWEVEKNY+A +F+K  QL +I  + Q
Sbjct:  276 TDEKGAKSSFNSMSQLAWEVEKNYNAPDFKKVKQLKQITYRGQ 318

>gi|316936734|gb|ADU60361.1| glutamyl-tRNA reductase binding protein [Nicotiana
        tabacum]

          Length = 320

 Score =  289 bits (738), Expect = 1e-075
 Identities = 157/283 (55%), Positives = 192/283 (67%), Gaps = 12/283 (4%)
 Frame = -2

Query: 1174 RCCVSSSVEVAAPPQSIIISSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXCVGV 995
            +C VS    V+ P QS    + N KP  AEV+RTIMELSSVGT               GV
Sbjct:   45 KCAVS---VVSEPIQS--EKTNNGKPYPAEVSRTIMELSSVGTLSTPTQDGWPLG--FGV 97

Query:  994 RFAVDPQDGSPIVSLNPNHHFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPPSNP 815
            RFAVDP  G+P++ LN                  VQLEQ  GLRT QCTI  +L  P + 
Sbjct:   98 RFAVDPH-GTPVLFLN---DATSKLSVNCKSSIHVQLEQ-YGLRTPQCTIQGTLEKPEDT 152

Query:  814 IHLNKLQSLWEKRFREKANSNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPLRDC 635
              L KL S+W+KRF  + + + + LL V+RVLQL++F +DG+W++S DY LANPDPLRD 
Sbjct:  153 TALKKLHSVWKKRFGHEVDEDHLFLLSVERVLQLEDFAEDGIWVTSSDYKLANPDPLRDF 212

Query:  634 AQRLVDGFNTDNREDVLRFCNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEVRIP 455
            A+R+++  NT NRED+LRFCNIYVD   QVSDAKM+W+DRLGFDV   SP+NDVFE RIP
Sbjct:  213 AERMIEEINTHNREDILRFCNIYVDLDFQVSDAKMLWVDRLGFDVRFRSPQNDVFEARIP 272

Query:  454 FPREVTNEKGAKSSFNGMAQQAWEVEKNYHALEFQKATQLNRI 326
            FPREV +EKGAKSSFN M+Q AWEVEKN+HA EF+KA QL +I
Sbjct:  273 FPREVADEKGAKSSFNCMSQLAWEVEKNFHAAEFEKAKQLKKI 315

>gi|224113295|ref|XP_002316448.1| predicted protein [Populus trichocarpa]

          Length = 322

 Score =  278 bits (711), Expect = 2e-072
 Identities = 146/289 (50%), Positives = 192/289 (66%), Gaps = 10/289 (3%)
 Frame = -2

Query: 1183 FHRRCCVSSSVEVAAPPQSIIISSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXC 1004
            F  +C +S+   V+A P + ++ S N+KP  AE++RTIMELSSVGT              
Sbjct:   42 FSLKCSLST---VSAEPPTHVVGSSNDKPFPAEISRTIMELSSVGTLSTLTPDGWPLS-- 96

Query: 1003 VGVRFAVDPQDGSPIVSLNPNHHFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPP 824
            VGVRFAVD  DG+PI+ L+ +H               VQLEQ +G+RT QCTI  SL  P
Sbjct:   97 VGVRFAVD-DDGTPILCLSDSHR---QFSVDRRSSLHVQLEQ-SGMRTPQCTIQGSLDKP 151

Query:  823 SNPIHLNKLQSLWEKRFREKANSNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPL 644
             +   L +  S+W+KRF E+     I+++ ++RVLQ+++F +DGVW+SS DY  A+PDPL
Sbjct:  152 EDTKLLKRAHSMWKKRFGEEVKDELIYVVAMERVLQMEDFVEDGVWVSSSDYKNASPDPL 211

Query:  643 RDCAQRLVDGFNTDNREDVLRFCNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEV 464
            RD A+   +  N  N EDV RFCN+YVD   QVS+AKM+W+DRLGFD+ L+SP+   F+V
Sbjct:  212 RDFAEAFANEINNKNMEDVSRFCNVYVDLDFQVSEAKMIWVDRLGFDMRLWSPQKGTFDV 271

Query:  463 RIPFPREVTNEKGAKSSFNGMAQQAWEVEKNYHALEFQKATQLNRIASK 317
            RIPFPREVT+ KGAKSSFNGM+Q AWEVEKNY  L F+K  QL +I  K
Sbjct:  272 RIPFPREVTDVKGAKSSFNGMSQLAWEVEKNYQTLNFKKVQQLKQITHK 320

>gi|357467765|ref|XP_003604167.1| hypothetical protein MTR_4g006200 [Medicago
        truncatula]

          Length = 315

 Score =  263 bits (671), Expect = 8e-068
 Identities = 135/279 (48%), Positives = 180/279 (64%), Gaps = 3/279 (1%)
 Frame = -2

Query: 1156 SVEVAAPPQSIIISSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXCVGVRFAVDP 977
            SV V+   +     ++  KP  AEV+RTIM+L+  GT              VGVRF VD 
Sbjct:   40 SVTVSTQLEQSKTENKIKKPFPAEVSRTIMDLARTGTLSTLTQQGYPLG--VGVRFVVDS 97

Query:  976 QDGSPIVSLNPNHHFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPPSNPIHLNKL 797
            QDG+P    N N                V+  Q +GLRT QCT+  +L  P +P  + +L
Sbjct:   98 QDGNPFFYFNHNAIPTTNNNIDTPSSLHVKFLQ-SGLRTPQCTLQGTLTNPQDPALIKRL 156

Query:  796 QSLWEKRFREKANSNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPLRDCAQRLVD 617
             SLW+KRF E+ N +F++ + V RVL LD+F +DGVW +S DY  A PDPLR+ A+ LV 
Sbjct:  157 VSLWKKRFGEEVNQDFMYFIAVDRVLHLDDFQEDGVWFTSLDYKNAQPDPLREFAENLVA 216

Query:  616 GFNTDNREDVLRFCNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEVRIPFPREVT 437
              NT+N ED+ RFCN+YVD   QV++AK++W+DRLG D+ L+SP N +FEVRIPFPREVT
Sbjct:  217 EINTNNMEDITRFCNVYVDLDFQVTEAKVIWVDRLGLDMRLFSPNNGLFEVRIPFPREVT 276

Query:  436 NEKGAKSSFNGMAQQAWEVEKNYHALEFQKATQLNRIAS 320
            +EKGAKS+FN M+Q AWEVE+N+H L+F+K T+L  I S
Sbjct:  277 DEKGAKSTFNCMSQLAWEVERNFHPLDFEKVTELKHIKS 315

>gi|388518743|gb|AFK47433.1| unknown [Lotus japonicus]

          Length = 312

 Score =  260 bits (664), Expect = 5e-067
 Identities = 139/286 (48%), Positives = 177/286 (61%), Gaps = 13/286 (4%)
 Frame = -2

Query: 1153 VEVAAPPQSI--------IISSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXCVG 998
            + + AP  S+        + SS   KP  AEV+RTIMEL+ VGT              +G
Sbjct:   32 ISIGAPKCSVSSTQLEMELSSSNTTKPFPAEVSRTIMELARVGTLSTLTHEGWPLG--IG 89

Query:  997 VRFAVDPQDGSPIVSLNPNHHFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPPSN 818
            VRFAVDP++G+P      NH+              VQ  QC GLRT QCT+  +L  P +
Sbjct:   90 VRFAVDPEEGTPFFCF--NHNVISNNNDNTPSSLHVQFIQC-GLRTPQCTLQGTLTKPQD 146

Query:  817 PIHLNKLQSLWEKRFREKANSNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPLRD 638
             I   +L SLW KRF E+ + + ++++ V RVL LD+F + GVW++S DY  A PDPLR+
Sbjct:  147 QIKAKRLVSLWGKRFGEEVDEDSVYIIAVDRVLHLDDFQESGVWVTSLDYKNAQPDPLRE 206

Query:  637 CAQRLVDGFNTDNREDVLRFCNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEVRI 458
             A+ LV   NT+N EDV RFCN+YVD    VS AKMVW+DRLGFD+ L  P    FE RI
Sbjct:  207 FAEELVTEINTNNMEDVTRFCNVYVDLDFPVSQAKMVWVDRLGFDMRLSCPLQGTFEARI 266

Query:  457 PFPREVTNEKGAKSSFNGMAQQAWEVEKNYHALEFQKATQLNRIAS 320
            PFPREVT+EKGAKSSFN M+Q AWEVEKN+H  +FQK  QL ++ S
Sbjct:  267 PFPREVTDEKGAKSSFNCMSQLAWEVEKNFHPPDFQKVKQLKQVKS 312

>gi|297830820|ref|XP_002883292.1| hypothetical protein ARALYDRAFT_898561
        [Arabidopsis lyrata subsp. lyrata]

          Length = 314

 Score =  245 bits (625), Expect = 2e-062
 Identities = 126/264 (47%), Positives = 175/264 (66%), Gaps = 11/264 (4%)
 Frame = -2

Query: 1117 SSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXCVGVRFAVDPQDGSPIVSLNPNH 938
            ++  +KP  AEV+R+IMELSSVGT              VGVRFAVD +DG+P++ LN   
Sbjct:   53 TASTHKPFPAEVSRSIMELSSVGTLSTLTHDGWPLG--VGVRFAVD-KDGTPVLCLN--- 106

Query:  937 HFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPPSNPIHLNKLQSLWEKRFREKAN 758
                           VQLEQC GLRT QCTI  S+  P +   L +L + W ++F E+  
Sbjct:  107 ---RSFSPDKRSALHVQLEQC-GLRTPQCTIQGSIGRPGDDTVLKRLSATWREKFGEEVE 162

Query:  757 SNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPLRDCAQRLVDGFNTDNREDVLRF 578
             + ++++ V RVLQ+++F +DG+W++S DY  A+PDPLRD A+ +V+  NT+N ED+ RF
Sbjct:  163 EDSLYVVAVDRVLQMEDFMEDGIWVASSDYKNASPDPLRDVAEDIVNNINTNNMEDIFRF 222

Query:  577 CNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEVRIPFPREVTNEKGAKSSFNGMA 398
            CN+YVD    VS+ KM+W+DRLGFD+ ++SP   V++VRIPFP EVT+EKGAKSSFNGM+
Sbjct:  223 CNVYVDLDFVVSETKMIWMDRLGFDLRVWSPRG-VYDVRIPFPMEVTDEKGAKSSFNGMS 281

Query:  397 QQAWEVEKNYHALEFQKATQLNRI 326
            Q AWEVEK+Y   +F K     ++
Sbjct:  282 QLAWEVEKSYCPADFNKVKLFKQV 305

>gi|21593452|gb|AAM65419.1| unknown [Arabidopsis thaliana]

          Length = 317

 Score =  245 bits (624), Expect = 2e-062
 Identities = 127/264 (48%), Positives = 176/264 (66%), Gaps = 11/264 (4%)
 Frame = -2

Query: 1117 SSQNNKPSAAEVARTIMELSSVGTXXXXXXXXXXXXXCVGVRFAVDPQDGSPIVSLNPNH 938
            ++  +KP  AEV+R+IMELSSVGT              VGVRFAVD +DG+PI+ LN   
Sbjct:   53 TASTHKPFPAEVSRSIMELSSVGTLSTLTHDGWPLG--VGVRFAVD-KDGTPILCLN--- 106

Query:  937 HFXXXXXXXXXXXXXVQLEQCAGLRTTQCTIHASLHPPSNPIHLNKLQSLWEKRFREKAN 758
                           VQLEQC GLRT QCTI  S+  P +   L +L + W+++F E+  
Sbjct:  107 ---RSVSPDKRSALHVQLEQC-GLRTPQCTIQGSIGRPGDDTVLKRLSATWKEKFGEEVE 162

Query:  757 SNFIHLLHVQRVLQLDNFTQDGVWISSHDYTLANPDPLRDCAQRLVDGFNTDNREDVLRF 578
             + ++++ V RVLQ+++F +DG+W++S DY  A+PDPLRD A+ +V+  N +N ED+ RF
Sbjct:  163 EDSLYVVAVDRVLQMEDFMEDGIWVASSDYKNASPDPLRDIAEDIVNQINANNMEDIFRF 222

Query:  577 CNIYVDSHLQVSDAKMVWLDRLGFDVHLYSPENDVFEVRIPFPREVTNEKGAKSSFNGMA 398
            CN+YVD    VS+ KM+W+DRLGFD+ ++SP   V++VRIPFP EVT+EKGAKSSFNGM+
Sbjct:  223 CNVYVDLDFVVSETKMIWMDRLGFDLRVWSPRG-VYDVRIPFPMEVTDEKGAKSSFNGMS 281

Query:  397 QQAWEVEKNYHALEFQKATQLNRI 326
            Q AWEVEK+Y   +F K   L ++
Sbjct:  282 QLAWEVEKSYCPADFNKVKLLKQV 305

>gi|35187431|gb|AAQ84307.1| fiber protein Fb4 [Gossypium barbadense]

          Length = 185

 Score =  225 bits (573), Expect = 2e-056
 Identities = 104/179 (58%), Positives = 138/179 (77%)
 Frame = -2

Query: 862 TTQCTIHASLHPPSNPIHLNKLQSLWEKRFREKANSNFIHLLHVQRVLQLDNFTQDGVWI 683
           T QCTI  SL  P++   L    S+W+KRF E A+ + ++ + VQRVLQ+++  +DGVW+
Sbjct:   1 TPQCTIQGSLTKPADATILRWFDSVWKKRFGESADVDNLYTVDVQRVLQMEDLNEDGVWV 60

Query: 682 SSHDYTLANPDPLRDCAQRLVDGFNTDNREDVLRFCNIYVDSHLQVSDAKMVWLDRLGFD 503
           +S DY  ANPDPLR+ A+ +V+  NT+NREDV RFCN+YVD   QVS+AKM+W+D LGFD
Sbjct:  61 TSSDYKNANPDPLRNSAEEIVNEINTNNREDVHRFCNVYVDLDFQVSEAKMIWVDCLGFD 120

Query: 502 VHLYSPENDVFEVRIPFPREVTNEKGAKSSFNGMAQQAWEVEKNYHALEFQKATQLNRI 326
           + +YSP+  VF+VRIPFPREVT+EKGAKSSFNGM+Q AWEVEKN+HA +F+K   L +I
Sbjct: 121 LRIYSPQKGVFDVRIPFPREVTDEKGAKSSFNGMSQLAWEVEKNFHAPDFEKVKPLKKI 179

  Database: GenBank nr
    Posted date:  Thu Sep 27 19:07:00 2012
  Number of letters in database: 7,061,663,739
  Number of sequences in database:  20,571,509

Lambda     K     H
   0.267   0.041    0.140
Gapped
Lambda     K     H
   0.267   0.041    0.140
Matrix: blosum62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,204,719,736,290
Number of Sequences: 20571509
Number of Extensions: 5204719736290
Number of Successful Extensions: 1415759300
Number of sequences better than 0.0: 0