Simple sequence repeat (SSR)
>UN16161 CTTGTGTCTTGTTCAACTAGTGACTGCTCCACCCTCTCTCTCTCTTGGACAACATCAGGTTTCTCCAGCTGCACATATACTTTTGAACCCTGTC TTTGTAAACTGGGCTTCCTGAACGATGACATTGTAGACTTCTTTTTAAACTTTTCCAAGAAAAAAAATGGACTTTTTCGGACCCCAGTTCCTGA TGTTACCACTTGGTCAATATAGTATGCAATAAAGAAAACCATGAACCACTCCACAACCATGATTATTAATACATGACGCATCTCATTATTGCTG TCACTCAAATCTCCCCATCGCATTCCATCAGTTCCCATATAGTTTCCCGTAAAGGCATACTGTGAAAACTCATATAATCCAGCATATAGAGAAA ACCCTGGATATAACTCCATC |
Primers for UN16161 SSR
Primer pair | Forward primer (5'-3') | Reverse primer (5'-3') | Product | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence | Temperature | Size | Sequence | Temperature | Size | Size | Start | End | |
1 | TTGTTCAACTAGTGACTGCTCCA | 59.966 | 23 | ATGTCATCGTTCAGGAAGCC | 60.081 | 20 | 117 | 9 | 125 |
2 | TTGTTCAACTAGTGACTGCTCCA | 59.966 | 23 | CGTTCAGGAAGCCCAGTTTA | 60.241 | 20 | 110 | 9 | 118 |
3 | TTGTTCAACTAGTGACTGCTCCA | 59.966 | 23 | GAACTGGGGTCCGAAAAAGT | 60.34 | 20 | 176 | 9 | 184 |