Simple sequence repeat (SSR)
>UN08660 AAACAAAGTCACTGCTAAATAACCGGTTCTTTTTAGGATAACGGACTCGTCGTGCGTCATTTTAACCGCGTGGATGTATCCGAAAGACGAGTCG TGGCCGAGCATTTCGATGTAGACGAGGCGCAGAATGTATTCTTTCATTTTGCGTTTGGGGATGTCGGGTGAGTGGATTAGCTTTTTTAAGATCT CGGTTTCGCGTAAGACGATGCGGTCTTCTTCGGATTTGGATCGGGCTTCGCCGATGGATTTGATTAGGTCGAGAAACTCTTTGGATTGGGAGAA GGTGGAGGTGACTGTGTTGGTGCCGAGAGCGAGTTCTCGGCCTATTTTTTGGAGGTGTTCCATTGGATTTGGGATTGGGGGTGTTTGGATATGG GTGTTACAGTGTGTGTGTGTGTTTTTGTGTGTGTGAATTTGGGGTTTAGGTGGATCGAGATCAAATTGGGG |
Primers for UN08660 SSR
Primer pair | Forward primer (5'-3') | Reverse primer (5'-3') | Product | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence | Temperature | Size | Sequence | Temperature | Size | Size | Start | End | |
1 | TCTTCTTCGGATTTGGATCG | 60.148 | 20 | CCACCTAAACCCCAAATTCA | 59.657 | 20 | 218 | 212 | 429 |
2 | TTAAGATCTCGGTTTCGCGT | 59.845 | 20 | CCACCTAAACCCCAAATTCA | 59.657 | 20 | 250 | 180 | 429 |
3 | TCGGGTGAGTGGATTAGCTT | 59.694 | 20 | CCACCTAAACCCCAAATTCA | 59.657 | 20 | 272 | 158 | 429 |