Simple sequence repeat (SSR)
>UN00493 CAATAACCGCTAATCTCGGGTACTTTCTCTAAATAATTCATCGCAAGTCAGCACGGGGGATCATGCAGCTTGAGCTTCAGGTTCGGGTTGTGGT TGAGATGTTATTTGTTCGGCTTTATAAGAATTAGGAATGTACTTCCAACAACGCTTGTGGACACCGCTAATTTTCTTTGGAGCATCAGCAGCAG GTACGCCGTCTTTGTGAACAATCCCCCAAGCCTTCCCGTGCTTACCATCCTTGTAAAGATTAATCTTGGTGATCTGATAAGCAACACCAGACCA ATGAGTTTTAGCCATCTGATAACCAATACCCCAGTTTGGTAAAAACTGAGCTACCTCGAATAAATTCTTCTTCTTCTTTCTTTTTGGTTTTGTT TTTGTTGTTGTTGTTGAGGATGATTCGATGGGTTCTTCTTTTACTTTCGGTTCTGTCATTTTTTACG |
Primers for UN00493 SSR
Primer pair | Forward primer (5'-3') | Reverse primer (5'-3') | Product | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence | Temperature | Size | Sequence | Temperature | Size | Size | Start | End | |
1 | CGTCTTTGTGAACAATCCCC | 60.353 | 20 | AAAAGAAGAACCCATCGAATCA | 59.945 | 22 | 224 | 195 | 418 |
2 | CCGTCTTTGTGAACAATCCC | 60.353 | 20 | AAAAGAAGAACCCATCGAATCA | 59.945 | 22 | 225 | 194 | 418 |
3 | TTTTCTTTGGAGCATCAGCA | 59.542 | 20 | AAAAGAAGAACCCATCGAATCA | 59.945 | 22 | 254 | 165 | 418 |