Simple sequence repeat (SSR)
>UN05138 ATCAATGGACGTTACTCGCCGTGTAAAATTTCTTAAAACACAGCATTTCAGGTGTTGAATCCTCTTTGACCGAACTCTTTGATTGGCTTCACCT TGTAGTTATCTTTCGTATTCGCATCATCTCTATTATACAATTCAGTCCTAACATCCATAGTAACTAAGGACTCGGGGTTACAGTTGGCGAAAAA GAGCGAATACTCATTCGGGGACGTAACGGGGTACGACTTGTTAAAACTAGACTGGGGAGGGGGGGAAAGGTCTCTAAAAGTGAACAGTAACTTT ATAAACTGGGAATCAACTACACAAAAATTAGGGTTTTGTTGCAATTCTAACAACACTTGTATTAGTGACTCTTCTGAAAGTAGGAAAAACCCTA ACCGTGATGGATCTGGTTGCGACAAGGATGACGTCACCGAGACGGCGGAGATCGCGACGGAGACGAATCCGGTGTGTGTGAATCCGAATTTCTC GAAGAGGATCATCGGCCGGTTGTCGGATCGGATCTTGAGTGACTTGATCTCGGCTGATGATGGTGTGATGGTGAGGGCGAAGAGGAGGAGGATG ACGGTGAGGATCTTCATTTTTGGGGTGGCGCGTGAGGTGAAAAGTGGTGGCGCGTGGGGTAGGGAGGGTGTGTGTGTGTAAAATAGAGATGTGA AGACGTTTGTTACGATGTTAGTTAAGTGAGATGTGAATGGATGTGGGCCGTTGGATTGGGGTGTGATGACAGGGTGGGTTTGAGTTTGGAGTTT GGAGTTTGGAC |
Primers for UN05138 SSR
Primer pair | Forward primer (5'-3') | Reverse primer (5'-3') | Product | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence | Temperature | Size | Sequence | Temperature | Size | Size | Start | End | |
1 | TGACTTGATCTCGGCTGATG | 59.942 | 20 | CAAACTCAAACCCACCCTGT | 59.861 | 20 | 237 | 510 | 746 |
2 | AAGAGGAGGAGGATGACGGT | 60.073 | 20 | CAAACTCAAACCCACCCTGT | 59.861 | 20 | 197 | 550 | 746 |
3 | TGACTTGATCTCGGCTGATG | 59.942 | 20 | AACTCAAACCCACCCTGTCA | 60.399 | 20 | 235 | 510 | 744 |