Simple sequence repeat (SSR)
>UN81325 TGATTTTACGCCTCGAATGATTAAGATAATACGATACAACTACAACAACATATATCATACAAGGTACAAGAATGAACCTAAATTTAGCCGAAAA GTAGAGTAGAGGAATATGAGACCATGGACAAGCTTAATGTAGTCGAGTAACGCTATACAGTAAGCTATAGTTACAACAACAATGGAAGTTTATT GTTCCATTTATATACACCGATACAGGTGCATAGTCTTATTTTGAAAATTTCTAATCACCACCCGAAAATAATTCAACCTTCACTTTCTTCATGT ACATAAGTGATACATACCTATGCTTAAACTCGTGTTATAGCTTCTCTTTATGTGGACATGTACATTTTGCGTGATGTTCTGAAAGAGACTCTGC TTCTTTCTGAAGCTCTTTTATATAGTCGACCGCTAAATCCAACATGTCTGCTGTGTTTGTTTGCTTGTCCATATTTGGGACAAGCTCTTGTAGC TTCCTCATTCTTTCACTTATTCTGGTCCGTCTTACCCTCTCAGCGATGCTTCTTGGGTGAGTTGCACAACCCCTTTTTGCTCTGCTTCTTAAAG GCACAGAATCTTGAAATTGCAGCAGTTTATCCAACTCGGCTGTACTTGATGGCAAGCTCATGTGATGCACAAGCGTACTGGGAGCACGTATTCT CCCAACATCATTCTGATTTTCAGATGGTTTTAAACTCGATAACTTGCTTTGATCAATTTCTCCAAAGTCTTTCAAGAAACTATCAGATAAGATA TCCGAATCATCCCATGATTGTGAACCAAATCCTCCATCATCACGGCCACTTTTCATATCCATAACTTTGCTCTCATTTTCAGGGATACGCGGCA AAATACCAGAAGACGTTAGTGAACTAGATGTAAATCCCATTTCATTTTTCATTCTCTTTGTCGAGGAGAGCATTGAGTTCCCCATACCCCTACC ACCAACTTTGAAATCATCCAACGATCTCATCGCTGGAAAACCATTATCCATATCGAGCTGATCGAAAAACCCGGCTGGTGAGCTACTTTGCCTT AGCAAATTATTATTCCTTATATTATTCGAATCATTAGTATTAGTCTGATGATCCATTTGTGACTGAAACTGATTCTGTTGTTGTTGTTGTTGCT GCTGCATTTGGGATTGCATTGCAGGATTGATATTGTTCTGTTGGAGCTTCTGTTTAGCCATTTGATGTAACTGCTGCTGATAGAGATAATGTGA GTTCGGATTTTGTTGTTGTTGTTGTTGTTGTCGTCGTCGTCGTCTGACATTAAATCTTCAACCGATTGAGATGGAATGTCCAAGCTATCATTTT TCTCTCCTGGTTTTGAGACTAGAGCTTCTTCTGGTTGAGCTAGAATTCCAGGATCCACTCTCTTTTTAATTCCTGGAATGCCCAATATTTCTTT GCCACGTCGAGTATGCTTTGGTCCATCAGAATAGGTCAACTGTTTCTTGAAACTTGGAGAATGCTTTCCCGACCACTTTCTCTTGCCAGTTGGA GAATGTTTTTTTGTGGACGAAGTTATTGCCTTGTCACCGATGTGATTCATTCTGAGCAGGTAAGA |
Primers for UN81325 SSR
Primer pair | Forward primer (5'-3') | Reverse primer (5'-3') | Product | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence | Temperature | Size | Sequence | Temperature | Size | Size | Start | End | |
1 | GCTGGTGAGCTACTTTGCCT | 59.644 | 20 | ACGACGACGACAACAACAAC | 59.648 | 20 | 249 | 1014 | 1262 |
2 | ATCGAGCTGATCGAAAAACC | 59.273 | 20 | ACGACGACGACAACAACAAC | 59.648 | 20 | 271 | 992 | 1262 |
3 | GCTGGTGAGCTACTTTGCCT | 59.644 | 20 | GACGACGACGACAACAACAA | 60.767 | 20 | 250 | 1014 | 1263 |