Simple sequence repeat (SSR)
>UN00308 ACCATTACCAACTACTTTTGTGACCATACATGCTAAAACATACCGTTATTCCGATGACATTTTTTTCGTGTCTTTTTCTTGCTGCGCTGGCCTG TTTCACGTTGAATGATTTTGGGAATAATGTCGTTTCAGCTTCTCTTTTAGCTTCATCAATGGAGAGGTGAAAACCTCGTATCGGAGGTGGTTTG TTGGTAGAGAGACTAAGTCGATGTAAAGCTGGTTGTATGCACTTCCGCTACAACATTGAGACACTTTTTATACTGAATCCTCATGATGGGATTA ATGCCTTTTATACTAACAAACCCTCTCAACTGATTATCCCCGTATCTCAGGTAAAAATATTAGTTGCAGATGCAGGAGTCCGGCGGCTAGACCA ACGCGTCTCAACTTGCTCCATATGTTTTTTGGCGCTTGTTCCTCTTTCTTTGACTCTTCTTGTTTCAATGTAACTGTTGCATAGAAACCATGAA TTGCCGCGTTGCGACAGGGCTTTTTAGTGTAACATAGTGATATTATTTCGTGACGACAGTTGATGTTATTGGGTAATGTTGATGGGTCACGGAT CAGTGGCGCACGGGTCAGTGAGTTGGATCTTGTTTGACCGAGCCTGTTATTGGGTATTTGTTGTGAGCCGGATTCTTGACACTGCATGTGCGGA GATTAACTTTGGAGCTCTTCTTCTAGGAACTGTTTTCACTTCTTTGTCATAACCAAAAAACGGTTGGTAATGCTGACAAAGTTGGTCACAGTTT CGCATGTACATATATTTTACTGGAGGGCCCGACACTGGTAGCTCATAGCTGCATAGTAACGGGTATGGTTAAGTTAGTATTAGATATCCAACAC ACCGGCGATCCTTTGAAGGAAGAAATGAGTTATCGAATAACCTTGTTGACTGCCATGTATCTGACTATTATCTGCGGATGAACCGGACTAATTT ACCTGTGCATGATTGGTAACACCTGCAATTAATGGTTATATGTATTCACTACGACAACTGTCAGCTGTGACATGTGTTTGTCTATGGTATACTT ACACCTTGAGTAACACATTCCACGTTTCTATAGCCAGAATCATTGAAAGATAAGGTCTTTTCGACATGTGCATATTCATTCGAGCATGGATAGT AGTTGTATCAGCCAAAGGAAACTTCGGCGCTGAAATTGTATGAGTGATACATCTGTCGCTGTTATACAAATACCATATTTATTTTAGTCTACCC GTGGTTCCCGTCGATAGCTTCTGATATTATGCATACTGCTCGCAATTGGTCAGGCAAATATACTTTGAAGCAGGCCCTCGAGTACTGAAGGTGC ACGCCAATCATTTTCCTGTAGAGTGGTCAAAACGGTTGCTGTTTGTGTAGGCATACGTCTGCTGACCAAGCGCGTTCGTCTATTATTTTCATGT AATGTGGTGGGACGTGCTTCCCCGTGCGACGTTGCATGTAAAATGACGAACAACTACGGCCAAAGGCGTTGCACGACAATTATGGGCATGCAAA GCGCGACAAACACTTTCCAAATCTTTGACCCCGTGCGAACTGCTGCATATATTTTGCTTTGGTTCATTTATGCCTGATGTGCCACATCGTGAGA TAATTCAGTCATTTTTATACTCATGAATGTCATTTCAGCTCGTGCCTTAACATCTCTGAGCTGAGCTTCCTTGCTTCCATAATTTGAAGGAGGA ATAATTTATAGCTACCTCTTGAGTTAGTCCTGGCATGACTTCATCCTCTTACTTGCCTCTTCAACCAATAAGATCGTCGCGGGAAATAAATTTA ACAGACATGGAAAGACTTCACATCATAGATTTCTTAGATTCACTTTTGCGTCTAATAGGGTAAGCTGCAAAAATCATCAGCAAGGCAGCCTTTT GGTGATCTCTTGTTTTATGAAGAAGATTTGATGAGATATCGTATGTAAAACTGGTTCTTGAATCAGTGAAGTTACTCATCACATATGGGGTGTA CCGGCCTTCTTGACCCCACCGGAATCCTACCTACAACGTCGTTGCCAACGAAAAAATACGACGGAATTTGTTCGGTGTGTCGGAGTGTGTGTGT GTTTTTTTTGAGTATTTTGTTGGGAAGAAGATTCAAGAAGAAAATGGAAGGGGTGGATTTATTAGAGATGAGAGAAGCTTCAAGAGAGGAAGCA AAAGGCAACTGTTTAGTACAAATGAGTAAAAAAAAAATTGAAGAATCACATACCTCTCAGCAAGTGGGGTGTGT |
Primers for UN00308 SSR
Primer pair | Forward primer (5'-3') | Reverse primer (5'-3') | Product | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence | Temperature | Size | Sequence | Temperature | Size | Size | Start | End | |
1 | GCTGCAAAAATCATCAGCAA | 59.96 | 20 | AATCCACCCCTTCCATTTTC | 59.996 | 20 | 278 | 1850 | 2127 |
2 | ATACGACGGAATTTGTTCGG | 59.823 | 20 | CAGTTGCCTTTTGCTTCCTC | 59.993 | 20 | 144 | 2030 | 2173 |
3 | CAAAAATCATCAGCAAGGCA | 59.809 | 20 | AATCCACCCCTTCCATTTTC | 59.996 | 20 | 274 | 1854 | 2127 |