Simple sequence repeat (SSR)
>UN65481 CTACTAAAGGTTAGAGTACATGTCAATTGACAATAACATGATTTGATGCTTCGCGATAAAATTCAACGGTCGAGCGAAACCTTGTAAAAGCATC CGTAATGCTAACTTAAGAAAAGACTTTACTATTCTAATAACACGTGAATGATAAATAATTGATGCATACTGTATATAATCTTTATACAATTTAT ATATGCAAGCACCTCCACACCACTACGTACACCACTATACAACTAATAATACAAGCGTAATCATCAATCATTCATACTTTGATGAAGACGTGAG ATGAAATGGCGTGCTAACTACAGGAAGTTCATGAACAACCTAGGCCAACCTACACTGACTTTCGTCACACGTTCTACTTCATTCGAGCATGTAG ACGATGTTGTTGTTGTTGTCGTTGCACCATGCTCGAAACTAACCCGACCATCGTCATCACCATTTCTAGCAGCCGTTGCAGTTTGTTTAGGACA AAGGAAGAAATTTCGTGAACCTAAGTCAATCAATTTGTCATCTGGCTCCAAACTTTGCAAGCTAAATTGCGAGTAATGAAGACTAAATTCAGAA GGATCAAGAGACGATAAAATCGGTCTTCTGCCTTCTTTCGAGTATTGTCGTAAAGCATCTGCCACAAGATCTCTAACACTCGAATTCGGTGATA TTATAACTTGCACTGGACCTAAGCTTCTTTGAACCGTAACTTTAAGCAATAACTTTGTTAATTTCGGACGCAACATATCCATCGACGAAACATT AGAAATTAAATCTGGAACAGTCTTGGGTCTCCTTAGTTTTCCTACCATGTCTTCAGCAGCATGACCATTAAACGACATAGATTTGTCTGACAAT CTCGCGTTCTTGCTCTTGTTCGTGTTTTTGTCTTGGTCTTTTCGGCTACTAATCTTCTTAGTCGACATTGATATGTCGAAAATCGTACTCTGTG GACTATAGTTTGAGATACATAACAAGCTAGGAATAATGTTGAGAGATCGAACAAGGGTTTAGAGATGAAATGATTTTAAGAAACAAGGTGGATA CGTGATTGTATATATAGTAGATGATTGGTTTACGTTCATTGGTTACGGGTTATTGGCGGTAGAGAATGTTTTTTACCCTTTTTTGTTGTGG |
Primers for UN65481 SSR
Primer pair | Forward primer (5'-3') | Reverse primer (5'-3') | Product | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence | Temperature | Size | Sequence | Temperature | Size | Size | Start | End | |
1 | CAAGCACCTCCACACCACTA | 59.745 | 20 | TAAACAAACTGCAACGGCTG | 59.911 | 20 | 272 | 194 | 465 |
2 | TGAGATGAAATGGCGTGCTA | 60.366 | 20 | TAAACAAACTGCAACGGCTG | 59.911 | 20 | 187 | 279 | 465 |
3 | CAAGCACCTCCACACCACTA | 59.745 | 20 | CGGCTGCTAGAAATGGTGAT | 60.235 | 20 | 258 | 194 | 451 |