Simple sequence repeat (SSR)
>UN53803 AATGTAACGCACAGATTTAGAAAAAAGATTGGATGACAAGTCCATTTTGAAGCCCCACCTTTAACACCTCTTCAAAGATGTACACAACCTTTAT TGCATAGGGCCAATCTTCACCACCAACATGAGCCCCACAAAGCAAAATTTGTGGGTCCCACAAGCAAAATTTATGACCCCACAAATCAAATTGT CACAGTTAGGATCTATTTACAACCACATTGAACTCTTGTGAATAACACAATGTATAGGATCAGAGGAAACTTATTGATCATCACATCAATGTTG TGCATAGGTTGGGGCCGGGCGGATGGCTGATGTGGCATACATGTCAGCATTAACGAGAGCTGCTGATTCTAGGAGGAACCGTGGAAATGCCTTG TCTGGAGAGCTCAGAAATGACGCTCTCCGTTGATGGAGGTTTGAGGCCTAAAGGCAAGGGTAGCCATGGCTTATTTATTGCTTCCTTTACAACC TTGTCAATGACTTCATCTCCCGGATAATTGTCGAGTGAGTTCTTTGGCATGGTCTGGCCACTCTGCATTGAATTCCTACTATGATATTGAGAAG CATTTTGAGGAAAAGATGGATGTGCCTGTAAAGTCGGGCAGCCCCATGCTTCGGCTTGCACCCCTGGATAAGGATTCCACATCCAATTTTCGGA AGAATGCCATGCTTGATAATACGGTGGGCTCCACATCTGAGGTGGTGGAGCATAATTGGTCGGAGGTGGCCAAGCAGGATAGACTTGGTTGGCT GGAAGGGTGTGATTAGAATGATATGGAGGGTAAGCCATTATGGGCCTCGGCGAATAGTAGTTTCTTGGTGTTGAATGTCTGGTTTGTGGCCATT TTCGCGCACTTTCTTTTGGCAATATATGTCTTCGTTGCAATCTATATTTCTGCAGATGACTTGCTACATTATGCCGAGTCAAACCCTCTACATT CATAAGTTCAAGTATACGAGAAGGTATTGCTTGATCAACACCGAGTTGCTCTACAGCTTGTACGAATTTCTTGTGTAGTTCAGAAGTCCAATCT ACCTTGATCTTTCTTCGACCTGATTTAGTAGCACGTGAACTAGGATTCTCAGATACTTCATTTGGCTTCTTACCATCATCTTTAGGATCAGGGC TGCATTCACTAGTAAACGGCTTGAGAATACCCTCCTCCAAACTCTTGGATGAAGCAGTCTCGGTAATAACAGTAGTATAGACAACTAAATCTTT ACAAATAGTTTCGACAAATTTGGATGGATCATCATGATCTACACACTCTTTCTCGGTCGAGAGATTAGTTTGAACTTGATCATGATCATGCTCA TGATCTTGACAGTCACCATTATCAACTAGCCTAATTCCATGCTTCAATTGGGGGGTTGATGGAGCCGGATATTTATCACCTTCAACAGATACCT CTACATTATGTTCTTGATTTTGACTAACCTCTACCTTTATATCTGTTTTCAAATGAACCTCTGGAGCCGATGCATTCTTAGATTTCAATGCTTC AGCTTGATCCTTTGGTCCCGCATTAAACGCCTTGTGAGCCACATGTTGCCATATGTTTCTAAGTTTATCTTCTGAAAGTGGTTTCTCGAGGAAT TCGACCGCACCGAGCGCTATGCACTTCATTATGGTACTAATGCAATTGACATTTGAAGTCATGATTGTAGGCAACTCTTTTGCACTTTCAAGAA ACTTAAACCTTCCATCTTCGTTGTCTGCCCTCACCTCTACAATTGCTACATGGAAGCTTCTAGATTTATCTGAAACGGCTGACAATGCTTCATT CGCAGTATAGAACATCGTGACAACATAATCCATTTCCTCAAGTTTTGATTTTATTTCATTTGCAGAATTGATATCTTCATCAAGTAGCAAGACT CTAAGACCCTTTGGAAAGTCTTTCCACTCCAATAAGTCGTTCGCAGTGCAAACCATAGCTGGTTCGGCATCCTTTATTTCATCAGATCTCTCTA TACAACATCACTATCTAATCTAAAATTCTTTACTAAAAGGATTAAAGAAAAGCTATGGGTGCCACTATGATGCTCTGCTTTTCCTTGTGATGGT GAGAGAGAGAGAGAGAGAGGTTCGGGATTTATGGAGCAGGATGAGAGCACAGAGTCTATGTAAA |
Primers for UN53803 SSR
Primer pair | Forward primer (5'-3') | Reverse primer (5'-3') | Product | ||||||
---|---|---|---|---|---|---|---|---|---|
Sequence | Temperature | Size | Sequence | Temperature | Size | Size | Start | End | |
1 | AAGAAAAGCTATGGGTGCCA | 59.708 | 20 | GTGCTCTCATCCTGCTCCAT | 60.381 | 20 | 100 | 2019 | 2118 |
2 | TGGTTCGGCATCCTTTATTT | 59.411 | 20 | GTGCTCTCATCCTGCTCCAT | 60.381 | 20 | 179 | 1940 | 2118 |
3 | CCAATAAGTCGTTCGCAGTG | 59.342 | 20 | GTGCTCTCATCCTGCTCCAT | 60.381 | 20 | 210 | 1909 | 2118 |